Factors affecting early identification of pregnant women by community health workers in Morogoro, Tanzania.

BACKGROUND: It is recommended that Antenatal Care (ANC) be initiated within the first trimester of pregnancy for essential interventions, such folic acid supplementation, to be effective. In Tanzania, only 24% of mothers attend their first ANC appointment during their first trimester. Studies have shown that women who have had contact with a health worker are more likely to attend their first antenatal care appointment earlier in pregnancy. Community health workers (CHWs) are in an opportune position to be this contact. This study explored CHW experiences with identifying women early in gestation to refer them to facility-based antenatal care services in Morogoro, Tanzania. METHODS: This qualitative study employed 10 semi-structured focus group discussions, 5 with 34 CHWs and 5 with 34 recently delivered women in three districts in Morogoro, Tanzania. A thematic analytical approach was used to identify emerging themes among the CHW and RDW responses. RESULTS: Study findings show CHWs play a major role in identifying pregnant women in their communities and linking them with health facilities. Lack of trust and other factors, however, affect early pregnancy identification by the CHWs. They utilize several methods to identify pregnant women, including: asking direct questions to households when collecting information on the national census, conducting frequent household visits and getting information about pregnant women from health facilities. CONCLUSIONS: We present a framework for the interaction of factors that affect CHWs' ability to identify pregnant women early in gestation. Further studies need to be conducted investigating optimal workload for CHWs, as well as reasons pregnant women might conceal their pregnancies.

Comparison of costs of home and facility-based basic obstetric care in rural Bangladesh.

This study compared the costs of providing antenatal, delivery and postnatal care in the home and in a basic obstetric facility in rural Bangladesh. The average costs were estimated by interviewing midwives and from institutional records. The main determinants of cost in each setting were also assessed. The cost of basic obstetric care in the home and in a facility was very similar, although care in the home was cheaper. Deliveries in the home took more time but this was offset by the capital costs associated with facility-based care. As use-rates increase, deliveries in a facility will become cheaper. Antenatal and postnatal care was much cheaper to provide in the facility than in the home. Facility-based delivery care is likely to be a cheaper and more feasible method for the care provider as demand rises. In settings where skilled attendance rates are very low, home-based care will be cheaper.

Perceived delay in healthcare-seeking for episodes of serious illness and its implications for safe motherhood interventions in rural Bangladesh.

Delay in accessing emergency obstetric-care facilities during life-threatening obstetric complications is a significant determinant of high maternal mortality in developing countries. To examine the factors associated with delays in seeking care for episodes of serious illness and their possible implications for safe motherhood interventions in rural Bangladesh, a cross-sectional study was initiated in Matlab sub-district on the perceptions of household heads regarding delays in seeking care for episodes of serious illness among household members. Of 2,177 households in the study, 881 (40.5%) reported at least one household member who experienced an illness perceived to be serious enough to warrant care-seeking either from health facilities or from providers. Of these, 775 (88.0%) actually visited some providers for treatment, of whom 79.1% used transport. Overall, 69.3% perceived a delay in deciding to seek care, while 12.1% and 24.6% perceived a delay in accessing transport and in reaching the provider respectively. The median time required to make a decision to seek care was 72 minutes, while the same was 10 minutes to get transport and 80 minutes to reach a facility or a provider. Time to decide to seek care was shortest for pregnancy-related conditions and longest for illnesses classified as chronic, while time to reach a facility was longest for pregnancy-related illnesses and shortest for illnesses classified as acute. However, the perceived delay in seeking care did not differ significantly across socioeconomic levels or gender categories but differed significantly between those seeking care from informal providers compared to formal providers. Reasons for the delay included waiting time for results of informal treatment, inability to judge the graveness of disease, and lack of money. For pregnancy-related morbidities, 45% reported 'inability to judge the graveness of the situation' as a reason for delay in making decision. After controlling for possible confounders in multivariate analysis, type of illness and facility visited were the strongest determinants of delay in making decision to seek care. To reduce delays in making decision to seek care in rural Bangladesh, safe motherhood interventions should intensify behaviour change-communication efforts to educate communities to recognize pregnancy-danger signs for which a prompt action must be taken to save life. This strategy should be combined with efforts to train community-based skilled birth attendants, upgrading public facilities to provide emergency obstetric care, introduce voucher schemes to improve access by the poorest of the poor, and improve the quality of care at all levels.

HIV and infant feeding practices: epidemiological implications for sub-Saharan African countries.

In industrialized countries HIV-1-seropositive mothers who are nursing infants are advised to use artificial feeds, whilst HIV-infected women in the developing world are recommended to breast-feed. Current evidence is insufficient even to estimate the attributable risk associated with breast-feeding. There is a possibility that the policy promoted in industrialized societies will eventually become established in urban and peri-urban areas of sub-Saharan Africa. This may be defensible for some elite urban mothers providing safe artificial feeding. However, calculations of the consequence of any population-level change to bottle-feeding indicate that it would result in more deaths from infectious causes, substantially adding to the child deaths directly attributable to HIV-1 infection. These data demonstrate that there is a clear need for policy-makers and health care workers to undertake further promotion of breast-feeding despite the AIDS epidemic.

PIP: The 3 retrospective studies conducted to date have involved a total of 12 children exposed to human immunodeficiency virus (HIV) via breastfeeding have yielded findings of 8 cases in which breastfeeding did appear to have led to HIV transmission and 4 cases where the children did not become infected. These findings, as well as the detection of HIV-1 in the breast milk of 3 women, have led to a policy in most developed countries that HIV-positive mothers are advised to use artificial milk. Of concern, however, is the possibility that this policy may become adopted in sub-Saharan African countries where child mortality from infectious diseases associated with bottle-feeding greatly exceed the risks of HIV transmission through breastfeeding. Compared to exclusively breastfed infants, artificial feeding in sub- Saharan countries is associated with a 1.8-2.6 times greater risk of post perinatal mortality. To examine the impact of changes in infant feeding practices in this regions, a model sub-Saharan country with an infant mortality rate of 90/1000 live births (ignoring the effects of HIV) was constructed and a 10% rate of maternal HIV infection, a 30% vertical transmission rate of HIV, and a 20 % HIV-associated infant mortality rate were assumed. To allow for competing causes of death, the number of infants dying from HIV infection was reduced by 10%. Calculations suggest that a reduction from 90% to 75% in the prevalence of breastfeeding would result in an increase in infant wastage of 1780 at best and 3580 at worst. The maximum (assuming a transmission rate of 100%) saving in infant wastage if breastfeeding were entirely eliminated would be only 630. Although more research is needed on the true level of risk from breastfeeding by HIV-infected mothers, it i s recommended that breastfeeding should be continued in developing countries where artificial feeding does not present a safe alternative, irrespective of the prevalence of HIV-1.

The impact of HIV-1 infection on mortality in children under 5 years of age in sub-Saharan Africa: a demographic and epidemiologic analysis.

OBJECTIVE: To estimate the effects of the HIV-1 epidemic on mortality in children under 5 years of age in urban and rural populations in eastern and central, and southern Africa. METHODS: A lifetable method that allows for the effects of competing causes (i.e., mortality due to HIV and other causes) was used to estimate mortality. Our calculations used published and unpublished data on HIV-1 infection in African adults and children (incidence and prevalence, vertical transmissions, transmission by blood transfusion and natural history), and typical baseline fertility and child mortality data. The results were applied to model rural and urban populations to explore the effects of parameters such as mortality in HIV-1-infected children, fertility in infected mothers and overall population growth. RESULTS: We estimate that child mortality will rise substantially because of the prevalence of HIV-1 in urban areas. There will be little difference in the absolute levels of increase in mortality between areas in eastern and central, and southern Africa with similar levels of HIV infection; however, in relative terms the effect will be more noticeable in southern Africa because of the lower baseline mortality. Towns with severe epidemics (30% adult seroprevalence) might experience a rise in child mortality of one-third in eastern and central Africa and three-quarters in southern Africa. This will cancel or reverse existing advantages in urban over rural levels of child mortality and this effect will be more pronounced in southern Africa. The exact impact of HIV-1 will vary according to mortality among HIV-1-infected children and to fertility among infected women. However, changes in age structure and population growth have relatively little impact on mortality. CONCLUSIONS: There are likely to be substantial increases in child mortality in sub-Saharan Africa as a result of HIV-1 infection. The main determinant of childhood infection is the scale of the epidemic among adults. Increases in mortality will depend on local adult seroprevalence but are hard to predict precisely because of possible variation in death rates among HIV-1-infected children. In rural areas with low seroprevalence other diseases will remain the main cause of mortality. However, in urban areas families and health services will have to face considerably increased demands from ill and dying children.

PIP: The objective was to estimate the effects of the HIV-1 epidemic on mortality in children under 5 years of age in urban and rural populations in eastern, central, and southern Africa. Epidemiologic parameters were applied to three populations with different characteristics: a rural population with an HIV-1 prevalence of 3%, an urban population (Town A) with 10% seroprevalence, and a second urban population (Town B) with 30% seroprevalence among women of childbearing age, respectively. The output measures were the absolute increases in and the level of the under-5-years' mortality rate, which represents the probability of a child dying before his or her fifth birthday per 1000 births. It was estimated that child mortality will rise substantially because of the prevalence of HIV-1 in urban areas. There will be little difference in the absolute levels of increase in mortality between areas in eastern, central, and southern Africa with similar levels of HIV infection; however, in relative terms the effect will be more noticeable in southern Africa because of the lower baseline mortality. Towns with severe epidemics (30% adult seroprevalence) might experience a rise in child mortality of 1/3 in eastern and central Africa and 3/4 in southern Africa. This will cancel or reverse existing advantages in urban over rural levels of child mortality with more pronounced effect in southern Africa. The exact impact of HIV-1 will vary according to mortality among HIV-1-infected children and according to fertility among infected women. The main determinant of childhood infection is the scale of the epidemic among adults. The educated urban elite can be expected to suffer from more young adult AIDS-related mortality. In rural areas with low seroprevalence, other diseases will remain the main cause of mortality. In urban areas, health services will have to face considerably increased demands from ill and dying children.

Prevalence of HIV-1 infection in the Kagera region of Tanzania: a population-based study.

A population-based survey was carried out in the Kagera region of the United Republic of Tanzania in 1987 to determine the magnitude of HIV-1 infection and to study associated risk factors. The region was divided into one urban and three rural zones. A multistage cluster sampling technique was adopted. Antibodies to HIV-1 were determined by enzyme-linked immunosorbent assay and confirmed by Western blot analysis. A total of 2,475 adults (aged 15-54 years) and 1,961 children (aged 0-14 years) was studied. The overall prevalence of HIV-1 infection among adults was 9.6%, with a higher prevalence in the urban zone (24.2%) than in the three rural zones (10.0, 4.5 and 0.4%, respectively). The corresponding figures for children were 1.3% overall: 3.9% in the urban area and for the rural areas 1.2, 0.8 and 0.0%, respectively. The age-specific seroprevalence for adults was highest in the age group 25-34 years. The age-standardized sex-specific prevalence was higher among women than men in the urban zone, while it was the same in the rural zones. Change of sexual partners among adults was associated with an increased risk of HIV-1 seropositivity. Travelling outside the region but within the country was also found to be associated with increased risk of HIV-1 infection but only in the rural population.

A community trial of the impact of improved sexually transmitted disease treatment on the HIV epidemic in rural Tanzania: 1. Design.

OBJECTIVE: To describe the rationale and design of a randomized trial of the impact of improved services for the treatment of sexually transmitted diseases (STD) on the incidence of HIV infection in Mwanza Region, Tanzania. METHODS: The likely impact of improved STD treatment services on HIV incidence, and the need for empirical information on the effectiveness of this intervention strategy, are discussed. The rationale and design of such an intervention programme in Mwanza Region, and of a community-randomized trial to measure the impact of the programme on HIV and other STD, are presented. Problems in the design and interpretation of the trial are reviewed. RESULTS: Results of the baseline survey of the cohort of over 12,000 adults in 12 communities are presented in a companion paper. CONCLUSION: There is an urgent need for effective preventive measures against the HIV epidemic in sub-Saharan Africa and other developing regions. Improved STD treatment has been promoted as a potentially effective strategy, but there is little empirical information on its impact. The trial in Mwanza Region is the first randomized study of this intervention and should provide valuable data for health policy makers.

PIP: Given the likelihood that other sexually transmitted diseases (STDs) act as co-factors in the sexual transmission of human immunodeficiency virus (HIV) in Africa, programs to improve the diagnosis and treatment of STDs may be an important component of acquired immunodeficiency syndrome (AIDS) control. To evaluate the impact of such a strategy, a randomized trial involving the integration of improved STD treatment into the existing primary health care system was initiated in Tanzania's Mwanza Region in late 1991. Program components include health personnel training, development of syndromic treatment algorithms, regular drug deliveries, supervisory visits to health facilities, and establishment of an STD reference clinic. The region's rural population was targeted due to its low yet increasing HIV prevalence, high prevalence of STDs, and amenability to a community-randomized study design. Twelve communities, defined as the population served by a health center and its satellite dispensaries, were selected for the trial and formed into six matched pairs on the basis of geographic area, HIV prevalence, and pre-existing levels of STD attendance. One set of communities was randomly selected to receive the intervention during the first year of the trial; the others will receive services at the end of the two-year follow-up period. Program outcome--defined as the incidence of HIV infection in intervention and control communities during the two-year follow-up--will be measured in a cohort of 12,000 randomly selected adults (1000 per community). This sample size offers a high power of detecting a halving of the annual HIV incidence rate from 1% to 0.5%.

The epidemiology of HIV-1 infection in urban areas, roadside settlements and rural villages in Mwanza Region, Tanzania.

OBJECTIVE: To determine the prevalence of HIV-1 infection and to identify the most important risk factors for infection. DESIGN: A cross-sectional population survey carried out in 1990 and 1991 in Mwanza Region, Tanzania. METHODS: Adults aged 15-54 years were selected from the region (population, 2 million) by stratified random cluster sampling: 2434 from 20 rural villages, 1157 from 20 roadside settlements and 1554 from 20 urban wards. Risk factor information was obtained from interviews. All sera were tested for HIV-1 antibodies using enzyme-linked immunosorbent assay (ELISA); sera non-negative on ELISA were also tested by Western blot. RESULTS: The response rate was 81%. HIV-1 infection was 1.5 times more common in women than in men; 2.5% of the adult population in rural villages, 7.3% in roadside settlements and 11.8% in town were infected. HIV-1 infection occurred mostly in women aged 15-34 years and men aged 25-44 years. It was associated with being separated or widowed, multiple sex partners, presence of syphilis antibodies, history of genital discharge or genital ulcer, travel to Mwanza town, and receiving injections during the previous 12 months, but not with male circumcision. CONCLUSION: This study confirms that HIV-1 infection in this region in East Africa is more common in women than in men. The results are consistent with the spread of HIV-1 infection along the main roads. There is no evidence that lack of circumcision is a risk factor in this population.

PIP: Between August 1990 and February 1991, health workers took blood samples from, and trained interviewers spoke to, 5145 15-to-54-year-old adults living in either an urban area (1554), in a rural village (2434), or in a roadside settlement (1157) in the Mwanza Region of Tanzania to determine the prevalence of HIV-1 infection and its most significant risk factors. The prevalence rate of HIV-1 infection was highest in urban areas, lower in roadside settlements, and lowest in rural areas (11.8%, 7.3%, and 2.5%, respectively), suggesting that HIV-1 had spread along main roads. Yet, there were as many people infected with HIV-1 in rural areas of the Mwanza Region as there were in Mwanza town. Women were 1.2 (rural) to 1.7 (urban) times more likely to be infected with HIV-1 than were men, indicating greater efficiency of HIV-1 transmission from men to women than from women to men. HIV-1 infection peaked in the 15-to-34-year-old group in women and in the 25-to-44-year-old group in men. Separated, divorced, or widowed men and women were at increased risk of being HIV-1 infected, even when controlled for numerous factors (odds ratio (OR) = 3.4 and 1.6, respectively). This may have been an indication of multiple partners, since the question concerning multiple partners was vague. Other important risk factors for women and men were syphilis antibodies (OR = 1.7 and 1.85), history of genital discharge or chancroid (OR = 2, 1.6 and 2.7, 1.6), travel to Mwanza town (OR = 2.1 and 1.7), and receiving injections during the previous 12 months (OR = 1.5 and 1.9). There was no link between male circumcision and HIV-1 infection. In fact, there seemed to be a moderate protective effect (OR = 0.8). This effect may be even more likely since urban men, who were at greatest risk of HIV-1 infection (8.7% vs. 5.4% [roadside] and 2.4% [rural]), had the highest rate of circumcision (61% vs. 29% and 17%, respectively).

The accuracy of an alternative confirmatory strategy for detection of antibodies to HIV-1: experience from a regional laboratory in Kagera, Tanzania.

BACKGROUND: Constant improvement of HIV tests often results in withdrawal of poorer quality tests by the manufacturing companies. It is thus often necessary to evaluate new HIV testing kits and modify the existing testing strategies. OBJECTIVES: To evaluate an alternative HIV antibody testing strategy which involves consecutive testing of sera by two enzyme-linked immunosorbent assays (ELISA), which both are recombinant antigen-based but utilise different test principles, followed by re-testing of sera giving discordant results. STUDY DESIGN: Sera (n = 1558) from a cross-sectional study of the HIV-1 seroprevalence in the Kagera region of Tanzania were tested using two ELISAs in parallel: Enzygnost anti-HIV-1/2 plus and Wellcozyme HIV-1 recombinant. Western blot analysis was done on all concordantly reactive and repeatedly discordant reactive samples as well as on 10% of concordantly ELISA negative sera. RESULTS: Two hundred and four sera (13.1%) were confirmed HIV-1-antibody positive. Both ELISAs had a sensitivity of 100%. The specificities of the ELISAs at initial and repeated testing were 99.8 and 99.9%, respectively, for Enzygnost and 97.7 and 99.5%, respectively, for Wellcozyme. None of the sera was concordantly false positive in both ELISAs. The mean ratio of the optical density of a sample to the cut off value of the test run (OD/CO ratio) was lower for samples giving false positive reactions than for confirmed HIV-1-antibody-positive samples. It is therefore important to interpret with caution HIV antibody ELISA test results on samples giving low OD/CO ratios. None of 10% of randomly selected concordantly ELISA negative sera gave a positive Western blot reaction. CONCLUSIONS: This field evaluation of an HIV antibody testing strategy involving the use of a recombinant antigen-based sandwich ELISA (Enzygnost) followed by a recombinant antigen-based competitive ELISA (Wellcozyme) showed that it had a sensitivity and specificity of 100%.

The protective effect of measles vaccine under routine vaccination conditions in Dar es Salaam, Tanzania: a case-control study.

A case-control study was conducted to determine the protective effect of measles vaccine under routine vaccination conditions in Dar es Salaam, Tanzania. A total of 172 cases of measles were obtained over two six-week periods from hospitals likely to admit such cases in the city and their mothers interviewed using a questionnaire that had been developed for the purpose. In addition, each case was matched to four controls for age, sex and place of residence and their mothers were also interviewed using the same questionnaire as for the cases. Vaccination status of the cases and the controls was ascertained by asking the mothers as well as obtaining vaccination information from the Mother-and-Child or Road-to-Health (MCH) clinic cards normally issued to mothers at first vaccination of their children. The overall protective effect of measles vaccine in all age groups studied was found to be 54% (95% confidence limits (CL)36%-67%) when mothers' recall for vaccination status was used. This level of protection was considered to be low compared with the efficacy of the vaccine under experimental conditions in controlled trials previously reported. However when MCH Clinic cards were used to ascertain vaccination status the protective effect rose to as high as 96% (95% CL 83%-99%). Potential biases in the study design and their role in masking or exaggerating the vaccine efficacy are discussed. It was therefore concluded that the protective effect of measles vaccine under routine vaccination conditions prevailing in Dar es Salaam region at the time of this study was as high as observed under experimental conditions when ascertainment of vaccination status was record-based. The study therefore recommended that, whenever in doubt about vaccine efficacy, simple study designs like the case-control method should be used to quickly evaluate vaccine efficacy by utilizing record-based vaccination status ascertainment methods.

Incidence of HIV-1 infection among adults in the Kagera region of Tanzania.

The results of a population-based follow-up study are presented. The baseline study which started in August 1987, was carried out to determine the prevalence of human immunodeficiency virus (HIV-1) infection in the Kagera region of Tanzania. A multistage cluster sampling technique was adopted in the selection of the study population. In the follow-up survey which started in June 1988, members of the same study population were revisited and studied in order to determine the incidence of HIV-1 infection among those who were HIV seronegative in the initial survey. HIV serology was conducted by using enzyme-linked immunosorbent assay and all positive sera were confirmed by the Western blot technique. A total population of 1316 adults aged 15-54 years was studied, constituting an average follow-up response rate of 69% in the rural areas and of 59% in the urban area. The overall incidence of HIV-1 infection among the adult population sample was 13.7 per 1000 person-years at risk with the highest incidence in the urban zone (47.5 per 1000 person-years at risk) and the lowest incidence in one of the three rural zones (4.9 per 1000 person-years at risk). The age-specific annual incidence was highest in the age group 25-34 years for males and in the age group 15-24 for females. From these results it was estimated that the number of newly infected adults in the region each year is about 8200 with a range between 5400 and 11,000 corresponding to the 95% confidence limits on the overall incidence.(ABSTRACT TRUNCATED AT 250 WORDS)

Socio-geographical patterns of HIV-1 transmission in Kagera Region, Tanzania.

A population-based study on the prevalence of HIV-1 infection in the Kagera region was performed in 1987. The study was based on a multistage cluster sampling technique covering the age-group 15-54 years. The study showed considerable variation of prevalence of HIV-1 infection in the areas studied. A total of 2475 adults were studied and the overall prevalence of HIV-1 infection in the region was 9.6% with a higher prevalence in the urban area (24.2%) compared to that in the rural areas (4.9%). In the present analysis, information on individuals collected during the prevalence study has been used to characterize smaller geographical areas of several villages (wards) and to form the basis for an aggregated analysis of geographical and social patterns relevant to HIV-1 transmission in the studied areas of the region. Kagera region is a heterogeneous society with respect to, for example, religion, ethnic groups and family patterns. There was a moderately strong positive correlation between HIV-1 prevalence and frequent change of sexual partners on the ward level. In Bukoba town the prevalence varied remarkably among the wards, with the lowest prevalence in the ward having the highest social status. In the rural areas, however, the prevalence in the wards decreased considerably with increasing distance from Bukoba town. This aggregated analysis of socio-geographical patterns of HIV-1 transmission characteristics has provided us with information for prioritizing areas of HIV/AIDS intervention in the Kagera community.

Communicating with the people about HIV infection risk as a basis for planning interventions: lessons from the Kagera Region of Tanzania.

In order to deepen the understanding of risk factors associated with HIV infection in the Kagera region of Tanzania and to investigate the potentials of communicating with the people in planning for interventions, two studies were performed in the districts of Bukoba Urban, Bukoba Rural and Muleba in 1989. The HIV prevalence of these areas ranged between 4.5% and 24.2% according to the prevalence study performed earlier in 1987. The studies involved the community in ward meetings on the one hand, and previously studied individuals on the other hand. The studies aimed both at conveying to the people the results of a previously performed study and at collecting new data using a combination of quantitative and qualitative methods in order to better understand the associated risk factors, perceived or real, and what suggestions the community could offer for reducing HIV transmission in the region. From the initial study, awareness about AIDS was found to be universal. Change of sexual partners and infection with syphilis were found to be the major risk factors for HIV-I infection. From the ward meetings people suggested a variety of solutions for interventions which we have categorized as either "hard" or "soft". The "hard" solutions involved suggestions such as isolation, imprisonment, castration and killing of AIDS victims, while the "soft" solutions involved sympathetic handling of the sick and educating the people about the modes of transmission and how best to prevent infection. There was a greater tendency for the low HIV prevalence rural communities to suggest the "hard" solutions than the high HIV prevalence urban ones which tended to suggest the "soft" solutions. However, with the changing dynamics of HIV infection in the region towards higher HIV prevalence in rural areas, it is likely that the "soft" solutions will gain acceptance and become adopted for interventions throughout the region. The information obtained from these studies has provided lessons that can be used for rational counselling as well as for guiding the implementation of IEC activities geared at interventions. It is also suggested that there should be further research into new strategies or their combinations which could be crucial in prevention such as those of community participation, empowerment of women and solidarity in AIDS intervention work.

Prevalence and incidence of syphilis and its association with HIV-1 infection in a population-based study in the Kagera region of Tanzania.

Population-based prevalence and incidence studies on HIV-1 infection were started in the Kagera region of Tanzania in 1987. The prevalence and incidence of infection with Treponema pallidum was studied to enable development of better strategies for STD control. Serological diagnosis of a past or treated infection with Troponema pallidum was made by seropositivity only to TPHA testing while active syphilis was diagnosed by seropositivity to both VDRL and TPHA tests. Seroconversion was measured in 1989 by finding TPHA serologically positive individuals during the follow-up period among the initially seronegative study population of 1987. The overall prevalence of active syphilis in the total sample of adults in the region was found to be 5.9% while that of past syphilis was 13.5%. The association between the prevalence of HIV-1 infection and syphilis of both types was found to be highly significant. However, the association between one pre-existing infection and seroconversion in the other was present but not statistically significant. The overall incidence of syphilis based on seroconversion in a cohort of adults in the region was found to be 11.6 per 1000 person-years at risk. In view of these findings, syphilis is a significant health problem in the region with a high level of transmission and efforts should be made to control it. Intervention studies should use these base-line data and monitor changes in syphilis incidence which may indicate changes in sexual behaviour. Such indicators could also be useful for evaluating the impact of interventions directed at reducing the transmission of HIV, syphilis and other STDs in the region.

PIP: Population-based prevalence and incidence studies on HIV-1 infection were started in the Kagera region of Tanzania in 1987. In 1989, a follow-up population-based serosurvey was conducted in the same population with the aim of determining the incidence of HIV-1 infection in the region. In 1987 multi-stage cluster sampling technique was employed to visit all the households selected and to choose at random 1 adult between 15 and 54 years old and 1 child under 15 years old. Syphilis serological examination was done on 2307 adults; 135 had active infection (both VDRL and TPHA positive), 1837 were negative, and 24 had false positive serology (VDRL positive and TPHA negative). The overall prevalence of active syphilis in the regional study sample was 5.9%, while that of past syphilis infection was 13.5%. The prevalence of past infection with syphilis in the rural areas (12.1%) was statistically significantly different from that in the urban area (17.8%, p 0.001). The prevalence of both types of infection increased with age in both rural and urban areas (p 0.001), except for active infection in the urban area (p 0.05). Analysis determining the association between HIV-1 infection and treponemal disease of both types found that there was a highly significant association between the 2 infections after controlling for the number of sexual partners in 3 categories of 0-1, 2-4, and 5 or more sexual partners an individual had had during the 8 years before 1987 (Mantel Haenszel weighted odds ratio 2.38). Between 1987 and 1989 the mean risk period for syphilis seroconversion of the 1133 individuals tested, was 1.82 years with a range between 1.20 and 2.49 years. The overall incidence was 11.6 per 1000 person-years at risk. The highest incidence was in the urban zone (15.4 per 1000 person-years at risk) and the lowest in the Karagwe rural zone (6.5 per 1000 person-years at risk). There was but a weak association between 1 pre-existing infection and seroconversion in the other as indicated by the risk ratios, which were more than unity.

Sentinel surveillance and cross sectional survey on HIV infection prevalence: a comparative study.

Despite widespread use of sentinel surveillance systems in monitoring the magnitude of HIV-1 infection in populations, little is known of how the trends they produce compare with those of the larger populations which they support to represent. The objective of this study, therefore, was to assess how useful sentinel surveillance data on HIV-1 infection might be in estimating the magnitude of HIV-1 infection in the general population. To achieve this, results from a population based study on HIV-1 infection in Bukoba town were compared with those from antenatal mothers and blood donors, all from the same town. The studies were done during the period of 1987-90. The overall HIV-1 prevalence was highest in the general population sample at 24.2% (95% CI 20.6-27.8) followed by that in antenatal mothers at 22.4% (95% CI 20.6-25.2) and lowest in blood donors at 11.9% (95% CI 9.1-15.3). Seroprevalence among antenatal clinic attenders was significantly lower than that of females from the general population sample (p = 0.016). Prevalence among female blood donors did not differ significantly from that of females from the general population sample (p = 0.06). Blood donor males had a lower HIV-1 seroprevalence when compared to that from the general population males (p = 0.038). The age group 25-34 years had the highest prevalence of HIV-1 infection in all the three populations indicating that this group is at the highest risk of HIV infection and that the three populations show a similar trend of age specific prevalence. From these findings, it is noted that female blood donors as a sentinel population represents more closely estimates of HIV-1 seroprevalence of females in the general population than antenatal clinic attenders or male blood donors. Further studies are proposed in different settings in order to come up with guidelines on the methodology of using sentinel surveillance populations in monitoring HIV-1 infection.

PIP: Researchers compared the results of a sentinel surveillance study on HIV-1 infection in 1292 pregnant women 15-47 years old attending prenatal care and in 454 blood donors (mean age = 28.7 years) in Bukoba, Tanzania, in 1990 with those from a cross sectional population-based study on HIV-1 infection among 553 people 15-54 years old also conducted in Bukoba during August 1987 to April 1988 to determine which sentinel populations most closely represented the HIV-1 infection rate of the general population. The HIV-1 prevalence rate was 24.4% for the population-based sample, 22.4% for the pregnant women, and 11.6% for the blood donors. The general population females had the highest HIV-1 prevalence rate, while the male blood donors had the lowest rate (29.4% vs. 10.5%). Pregnant women had a significantly lower rate than general population females (22.4% vs. 29.4%; p = 0.016). The 25-34 year old age group had the highest prevalence of HIV-1 infection in all three populations, suggesting that this group faces the highest risk of HIV infection and that the three populations have a similar trend of age-specific prevalence. There were no significant differences between the HIV-1 prevalence rates among general population females and those among female blood donors, suggesting that female blood donors more closely represent the HIV-1 seroprevalence rate of general population females than pregnant women attending prenatal care or male blood donors. There is a need for additional studies in different settings in order to establish guidelines on the methodology of using sentinel surveillance studies in monitoring HIV-1 infection.

Prevalence of intestinal parasites in adult patients with enteropathic AIDS in north-eastern Tanzania.

A six month study was conducted in north-eastern Tanzania to determine the prevalence of pathogenic intestinal parasites among adult patients with enteropathic AIDS. A total of 352 patients were recruited of whom 158 (45%) had chronic diarrhoea. Of the 352 patients, 123 (35%) had intestinal parasites. Of the 123, 77 (62.6%) patients had chronic diarrhoea. The types of parasites detected were Cryptosporidium, Isospora belli, Strongyloides stercoralis, Schistosoma mansoni, Trichuris, trichiura, Ascaris lumbricoides, hookworm and Entamoeba histolytica. The prevalence of intestinal parasites was significantly higher in patients with chronic diarrhoea than in those without (P < 0.05). Cryptosporidium and Isospora belli were only detected in patients with chronic diarrhoea and were thus the most likely cause of the diarrhoea. This study has established that coccidian parasites are the most important gut opportunistic infections in Tanzanian patients with enteropathic AIDS. The fact that a high proportion of patients with chronic diarrhoea (51.3%) had no identifiable parasitic agents, suggests that other infectious agents or alternative mechanisms other than infections are responsible for the diarrhoea.

PIP: A 6-month study was conducted in northeastern Tanzania to assess the prevalence of pathogenic intestinal parasites among adult patients with enteropathic AIDS. Of the 352 patients recruited, 158 had chronic diarrhea and 123 had intestinal parasites. 77 of the 123 patients with intestinal parasites had chronic diarrhea. Cryptosporidium, Isospora belli, Strongyloides stercoralis, Schistosoma mansoni, Trichuris trichiura, Ascaris lumbricoides, hookworm, and Entamoeba histolytica were detected. The prevalence of intestinal parasites was significantly higher in patients with chronic diarrhea than in those without. Cryptosporidium and Isospora belli were only detected in patients with chronic diarrhea and were therefore the most likely cause of the diarrhea. Coccidian parasites have thus been identified as the most important gut opportunistic infections in Tanzanian patients with enteropathic AIDS. However, that 51.3% of patients with chronic diarrhea had no identifiable parasitic agents suggests that other infectious agents or alternative mechanisms are responsible for the condition.

Use of the Automated Disaster and Emergency Planning Tool in developing district level public health emergency operating procedures in three East African countries.

BACKGROUND: Sub-Saharan Africa is vulnerable to several natural and man-made disasters. We used the CDC Automated Disaster and Emergency Planning Tool (ADEPT) to develop all-hazards disaster management plans at district level in three eastern African countries. METHODS: During July 2008-February 2011, we used the automated disaster and emergency planning tool to conduct training on disaster planning and management in the three east African countries namely Kenya, Tanzania and Uganda. We trained district disaster teams per country. We held 7 trainings in Tanzania, 8 in Uganda and 10 in Kenya respectively. The district disaster management teams trained comprised five district administrative personnel and a national Red Cross officer. The training took 5 days. RESULTS: A total of 100 districts teams (40 in Uganda and 35 in Kenya and Tanzania respectively) were trained using the ADEPT and consequently 100 district disaster response plans were developed during 2008-2011. A total 814 district disaster team members from these districts were trained. Our experience has shown that the Automated Disaster Emergency Planning Tool is a relatively quick, easy, practical, participatory and inexpensive approach to developing emergency operating plans at the sub-national (district) level. CONCLUSIONS: The ADEPT can be used relatively easily, quickly and inexpensively at the sub-national levels to develop emergency operating procedures to improve disaster management. Although the ADEPT enables district disaster response teams to generate their disaster response plans, the use of the ADEPT may be hampered by lack of computer skills and knowledge of MS computer programme by district personnel in resource limited settings.

Regional approach to building operational level capacity for disaster planning: the case of the Eastern Africa region.

BACKGROUND: The Eastern Africa region is regularly affected by a variety of disasters ranging from drought, to human conflict and population displacement. The magnitude of emergencies and response capacities is similar across the region. In order to strengthen public health disaster management capacities at the operational level in six countries of the Eastern Africa region, the USAID-funded leadership project worked through the HEALTH Alliance, a network of seven schools of public health from six countries in the region to train district-level teams. OBJECTIVES: To develop a sustainable regional approach to building operational level capacity for disaster planning. METHODS: This project was implemented through a higher education leadership initiative. Project activities were spear-headed by a network of Deans and Directors of public health schools within local universities in the Eastern Africa region. The leadership team envisioned a district-oriented systems change strategy. Pre-service and in-service curricula were developed regionally and district teams were formed to attend short training courses. Project activities began with a situational analysis of the disaster management capacity at national and operational levels. The next steps were chronologically the formation of country training teams and training of trainers, the development of a regional disaster management training curriculum and training materials, the cascading of training activities in the region, and the incorporation of emerging issues into the training curriculum. An evaluation model included the analysis of preparedness impact of the training program. RESULTS: The output from the district teams was the creation of individual district-level disaster plans and their implementation. This 4-year project focused on building operational level public health emergency response capacity, which had not previously been part of any national program. Use of the all-hazard approach rather than a scenario-based contingency planning led to the development of a standardized curriculum for training both in-service and pre-service personnel. Materials developed during the implementation phases of the project have been incorporated into public health graduate curricula in the seven schools. This systems-based strategy resulted in demonstrable outcomes related to district preparedness and university engagement in disaster management. CONCLUSION: University partnerships are an effective method to build district-level disaster planning capacity. Use of a regional network created a standardized approach across six countries.

Institutional frameworks for management of epizoonotic emergencies in six countries in the Eastern Africa region: a situational analysis.

INTRODUCTION: The Eastern Africa region is a hot-spot for epidemics of emerging zoonotic diseases ('epizoonotics'). However, the region's capacity for response to epidemics of zoonotic origin has not been documented. This paper presents a multi-country situational analysis on the institutional frameworks for management of zoonotic epidemics in the Eastern Africa region. METHODS: A multi-country assessment of 6 country teams was conducted (Uganda, Kenya, Tanzania, Ethiopia, DRC and Rwanda). It involved a review of records and interviews with key informants from agencies with a stake in the management of zoonotic and disasters in general in the respective countries. Qualitative data were analyzed for key emerging themes. FINDINGS: There are many socio-cultural risk factors to epidemic prone zoonotic diseases in the region. Countries have varying levels of preparedness for zoonotic emergencies. All 6 countries have a framework for disaster management. However, technical response to epidemics is managed by the line sectors, with limited Inter-sectoral collaboration. Some sectors were disproportionately more prepared than others. Surveillance systems are mostly passive and inadequate for early detection. All 6 countries have built reasonable capacity to respond to avian influenza, but not other zoonotic emergencies. Most countries lack personnel at the operational levels, and veterinary public health services are ill-facilitated. CONCLUSION: There is need to strengthen veterinary public health services at all levels, but with a 'one health' approach. There is also need to establish 'risk-based surveillance' hot spots for zoonotic epidemics and to build community resilience 'epizoonotic' diseases.