RESUMO
A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic of Korea. Cells of strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar. Strain N237T contained iso-C16â:â0 and C17â:â1ω8c as the major fatty acids, MK-9(H4) as the predominant isoprenoid quinone and meso-DAP as the diamino acid in the peptidoglycan. It contained diphosphatidylglycerol, phosphatidylinositol polymannosides, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified glycophospholipids, three unidentified glycolipids and two unidentified lipids as polar lipids. The DNA G+C content was 68.1 mol%. Strain N237T formed a separate lineage in the genus Jatrophihabitans, as demonstrated by phylogenetic analysis based on 16S rRNA sequencing. It was most closely related to Jatrophihabitans soli KIS75-12T (95.6â% sequence similarity). The combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain N237T represents a novel species in the genus Jatrophihabitans, for which the name Jatrophihabitans telluris sp. nov. is proposed. The type strain is N237T (=KCTC 39922T=NRRL B-65477T).
Assuntos
Actinomycetales/classificação , Florestas , Filogenia , Microbiologia do Solo , Áreas Alagadas , Actinomycetales/genética , Actinomycetales/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Peptidoglicano/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A novel bacterial strain, designated N60AT, was isolated from sediment soil of crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of N60AT were Gram-reaction-negative, oxidase- and catalase-positive, non-motile rods and formed transparent white colonies on ten-fold diluted R2A agar. N60AT contained summed feature 3 (C16â:â1ω7c/C16â:â1ω6c), iso-C15â:â0 and iso-C17â:â0 3-OH as the major fatty acids and MK-7 as the predominant isoprenoid quinone. It contained phosphatidylethanolamine as the predominant polar lipid. The DNA G+C content was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that N60AT formed a separate lineage in the genus Mucilaginibacter and that it was most closely related to Mucilaginibacter frigoritolerans FT22T (96.5â% sequence similarity). Phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that N60AT represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter craterilacus sp. nov. is proposed. The type strain is N60AT (=KCTC 52404T=NRRL B-65396T).
Assuntos
Bacteroidetes/classificação , Sedimentos Geológicos/microbiologia , Lagos/microbiologia , Fosfatidiletanolaminas/química , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNARESUMO
A bacterial strain, designated SN6-9T, was isolated from soil of the Gotjawal, lava forest, located in Jeju, Republic of Korea. Strain SN6-9T was Gram-stain-negative, motile, oxidase- and catalase-negative, yellow-pigmented and rod-shaped. It contained summed feature 3 (C16â:â1ω7c and/or C16â:â1ω6c) and summed feature 8 (C18â:â1ω7c and/or C18â:â1ω6c) as the major fatty acids, Q-10 as the predominant isoprenoid quinone, sym-homospermidine as the major polyamine and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and two unidentified phospholipids as the polar lipids. The DNA G+C content was 64.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a separate lineage in the genus Sphingomonas. Based on the results from this polyphasic taxonomic study, it is concluded that strain SN6-9T represents a novel species in the genus Sphingomonas. The name Sphingomonas gotjawalisoli sp. nov. is proposed; the type strain is SN6-9T (=KCTC 52405T=NRRL B-65395T).
Assuntos
Florestas , Filogenia , Microbiologia do Solo , Sphingomonas/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Sphingomonas/genética , Sphingomonas/isolamento & purificação , Ubiquinona/químicaRESUMO
A novel bacterial strain, Back-11T, was isolated from sediment soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain Back-11T were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- and catalase-positive. It contained anteiso-C15 : 0 as the major fatty acid, menaquinone-7 (MK-7) as the predominant isoprenoid quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified aminophospholipids as the main polar lipids, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 45.3âmol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain Back-11T was most closely related to Paenibacillus taihuensis THMBG22T (95.5 % similarity) and fell into a clade in the genus Paenibacillus. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain Back-11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillus baekrokdamisoli sp. nov. is proposed. The type strain is Back-11T ( = KCTC 33723T = CECT 8890T).
Assuntos
Lagos/microbiologia , Paenibacillus/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Paenibacillus/genética , Paenibacillus/isolamento & purificação , Peptidoglicano/química , Fosfatidiletanolaminas/química , Fosfatidilgliceróis/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A novel actinobacterial strain, B2-12T, was isolated from soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain B2-12T were Gram-stain-positive, non-motile, non-spore-forming and coccoid to short-rod-shaped. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain B2-12T belonged to the genus Nocardioides and shared highest sequence similarity with 'Nocardioidespaucivorans' KIS31-44 (98.4 %). The predominant isoprenoid quinone was MK-8(H4). The major fatty acids of strain B2-12T were C16 : 1 (ω7c and/or ω6c), summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), C17 : 0 10-methyl, iso-C16 : 0, C16 : 0 and C17 : 1ω6c. The diagnostic diamino acid in the cell-wall peptidoglycan was ll-diaminopimelic acid. It contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids. The DNA G+C content was 67.0 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, 16S rRNA gene sequence comparisons and DNA-DNA hybridization data, strain B2-12T represents a novel species in the genus Nocardioides, for which the name Nocardioidesbaekrokdamisolisp. nov. is proposed. The type strain is B2-12T (=KCTC 39748T=NRRL B-65313T=DSM 100725T).
Assuntos
Actinomycetales/classificação , Lagos/microbiologia , Filogenia , Microbiologia do Solo , Actinomycetales/genética , Actinomycetales/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Peptidoglicano/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A Gram-stain-negative, non-motile, yellow-pigmented and rod-shaped bacterial strain, designated SN6-13T, was isolated from soil of the Gotjawal, lava forest, located in Jeju, Republic of Korea. Cells of strain SN6-13T were oxidase- and catalase-positive. The isolate contained Q-10 as the predominant isoprenoid quinone, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 as the major fatty acids, sym-homospermidine as the major polyamine and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, ninhydrinphosphatidylglycerol and two unidentified aminophospholipids as the polar lipids. The DNA G+C content was 64.6âmol%. In phylogenetic analyses based on 16S rRNA gene sequencing, strain SN6-13T was most closely related to Sphingomonas laterariae LNB2T (95.4 % sequence similarity) and formed a separate lineage in the genus Sphingomonas. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, it is concluded that strain SN6-13T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas vulcanisoli sp. nov. is proposed. The type strain is SN6-13T ( = KCTC 42454T = CECT 8804T).
Assuntos
Florestas , Filogenia , Microbiologia do Solo , Sphingomonas/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Sphingomonas/genética , Sphingomonas/isolamento & purificação , Ubiquinona/químicaRESUMO
A novel bacterial strain, NKM-5(T), was isolated from soil of a lava forest in Nokkome Oreum, Jeju, Republic of Korea. Cells of strain NKM-5(T) were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- and catalase-positive. Strain NKM-5(T) contained anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids; menaquinone-7 (MK-7) as the predominant isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, an unidentified phospholipid and three unidentified aminophospholipids as the polar lipids; and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 48.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain NKM-5(T) was most closely related to Cohnella lupini RLAHU4B(T) (96.9% sequence similarity) and fell into a clade in the genus Cohnella. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain NKM-5(T) represents a novel species of the genus Cohnella, for which the name Cohnella collisoli sp. nov. is proposed. The type strain is NKM-5(T) ( = KCTC 33634(T) = CECT 8805(T)).
Assuntos
Bacillales , Microbiologia do Solo , Bacillales/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Florestas , Lisina , Dados de Sequência Molecular , Peptidoglicano/química , Fosfatidilgliceróis/análise , Fosfolipídeos/química , Filogenia , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Solo , Vitamina K 2/análogos & derivadosRESUMO
A novel bacterial strain, designated SA3-7(T), was isolated from soil of a lava forest located in Jeju, Republic of Korea. Cells of strain SA3-7(T) were Gram-stain-negative, oxidase- and catalase-positive, non-motile rods and produced creamy white colonies on ten-fold-diluted R2A agar. The isolate contained menaquinone-7 (MK-7) as the predominant isoprenoid quinone and summed feature 3 (C16â:â1ω7c/C16â:â1ω6c), iso-C15â:â0 and iso-C17â:â0 3-OH as the major fatty acids. The DNA G+C content was 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain SA3-7(T) was related most closely to Mucilaginibacter frigoritolerans FT22(T) (96.7â% sequence similarity) and that it formed a separate lineage in the genus Mucilaginibacter. Combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain SA3-7(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter gotjawali sp. nov. is proposed; the type strain is SA3-7(T) (â=âKCTC 32515(T)â=âCECT 8628(T)â=âDSM 29289(T)).
Assuntos
Bacteroidetes/classificação , Florestas , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
BACKGROUND: Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown. RESULTS: We investigated the anti-obesity effects of 6,6'-bieckol, 6,8'-bieckol, 8,8'-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCATT/enhancer-binding protein α (C/EBPα) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50 µg mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6'-bieckol markedly decreased lipid accumulation and expression levels of PPARγ, C/EBPα, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA). CONCLUSION: These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis.
Assuntos
Adipogenia/efeitos dos fármacos , Fármacos Antiobesidade/farmacologia , Dioxinas/farmacologia , Regulação para Baixo/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Células 3T3-L1 , Animais , Fármacos Antiobesidade/efeitos adversos , Fármacos Antiobesidade/química , Fármacos Antiobesidade/isolamento & purificação , Benzofuranos/efeitos adversos , Benzofuranos/química , Benzofuranos/isolamento & purificação , Benzofuranos/farmacologia , Carbono-Carbono Ligases/antagonistas & inibidores , Carbono-Carbono Ligases/genética , Carbono-Carbono Ligases/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Dioxinas/efeitos adversos , Dioxinas/química , Dioxinas/isolamento & purificação , Ácido Graxo Sintase Tipo I/antagonistas & inibidores , Ácido Graxo Sintase Tipo I/genética , Ácido Graxo Sintase Tipo I/metabolismo , Camundongos , Estrutura Molecular , PPAR gama/antagonistas & inibidores , PPAR gama/genética , PPAR gama/metabolismo , Oceano Pacífico , Phaeophyceae/química , República da Coreia , Alga Marinha/química , EstereoisomerismoRESUMO
A novel bacterial strain designated CB4(T) was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4(T) was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C(15:0) and iso-C(16:0), and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4(T) was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4(T) forms a deep branch within the genus Aneurinibacillus, sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562(T) (96.5%). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4(T) is considered to represent a novel species within the genus Aneurinibacillus, for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4(T) (â=KCTC 33505(T)â=CECT 8566(T)). An emended description of the genus Aneurinibacillus is also proposed.
Assuntos
Bacilos Gram-Positivos Formadores de Endosporo/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Bacilos Gram-Positivos Formadores de Endosporo/genética , Bacilos Gram-Positivos Formadores de Endosporo/isolamento & purificação , Dados de Sequência Molecular , Peptidoglicano/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A novel bacterial strain designated GJW-30(T) was isolated from soil of the lava forest, Gotjawal, located in Aewol, Jeju, Korea. Strain GJW-30(T) was found to be strictly aerobic, Gram-negative and to form pleomorphic, non-motile rods and white colonies on R2A agar. The major fatty acids were identified as C18:1ω7c, C16:0 and C17:0, the predominant isoprenoid quinone as Q-10, the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The cell-wall sugar pattern of strain GJW-30(T) was found to be composed of glucose, ribose and rhamnose and meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain GJW-30(T) is 62.2 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain GJW-30(T) forms a deep branch within the order Rhizobiales, sharing the highest level of sequence homology with Bradyrhizobium oligotrophicum LMG 10732(T) (93.6 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain GJW-30(T) is considered to represent a novel genus and species, for which the name Variibacter gotjawalensis gen. nov., sp. nov. (the type strain is GJW-30(T) = KCTC 32391(T) = CECT 8514(T) = LMG 28093(T)) is proposed.
Assuntos
Bacilos e Cocos Aeróbios Gram-Negativos/classificação , Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Carboidratos/análise , Parede Celular/química , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácido Diaminopimélico/análise , Ácidos Graxos/análise , Florestas , Bacilos e Cocos Aeróbios Gram-Negativos/genética , Bacilos e Cocos Aeróbios Gram-Negativos/fisiologia , Coreia (Geográfico) , Dados de Sequência Molecular , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
The purpose of this study was to determine whether leisure time physical activity (PA) affects risk for developing metabolic syndrome (MetS) among Korean adults. We studied 1728 Korean adults over age 20 years who visited a public health promotion center in Seoul, Korea. All subjects completed an International Physical Activity Questionnaire, and were examined for MetS criteria as defined by National Cholesterol Education Program's Adult Treatment Panel III criteria. The association between PA and MetS was assessed using multivariate logistic regression analysis. The odds ratios (95% confidence interval) for having MetS and engaging in vigorous PA, moderate PA or light PA (walking) compared with having MetS and not engaging in vigorous PA, moderate PA or light PA (walking) were 0.482 (0.322-0.721, p < 0.001), 0.547 (0.370-0.810, p = 0.003) and 0.482 (0.318-0.731, p < 0.001) for PA once a week; 0.763 (0.468-1.243, p = 0.277), 0.697 (0.419-1.160, p = 0.165) and 0.674 (0.403-1.128, p = 0.134) for PA twice a week; 0.449 (0.248-0.815, p = 0.008), 0.568 (0.338-0.954, p = 0.033) and 0.451 (0.260-0.780, p = 0.004) for PA 3 times a week; 0.321 (0.123-0.835, p = 0.020), 0.737 (0.362-1.502, p = 0.401) and 0.411 (0.185-0.916, p = 0.030) for PA 4 times a week; and 0.216 (0.064-0.727, p = 0.013), 0.390 (0.166-0.915, p = 0.031) and 0.318 (0.181-0.558, p < 0.001) for PA 5 or more times a week, respectively. These findings indicate vigorous PA, moderate PA and light PA may reduce the risk of MetS among Korean adults.
Assuntos
Exercício Físico , Síndrome Metabólica/epidemiologia , Adulto , Glicemia , Pressão Sanguínea , Pesos e Medidas Corporais , Estudos Transversais , Feminino , Comportamentos Relacionados com a Saúde , Humanos , Atividades de Lazer , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Razão de Chances , República da Coreia/epidemiologia , Fatores SocioeconômicosRESUMO
An irregular performance of a mechanical-type constant power regulator is considered. In order to find the cause of an irregular discharge flow at the cut-off pressure area, modeling and numerical simulations are performed to observe dynamic behavior of internal parts of the constant power regulator system for a swashplate-type axial piston pump. The commercial numerical simulation software AMESim is applied to model the mechanical-type regulator with hydraulic pump and simulate the performance of it. The validity of the simulation model of the constant power regulator system is verified by comparing simulation results with experiments. In order to find the cause of the irregular performance of the mechanical-type constant power regulator system, the behavior of main components such as the spool, sleeve, and counterbalance piston is investigated using computer simulation. The shape modification of the counterbalance piston is proposed to improve the undesirable performance of the mechanical-type constant power regulator. The performance improvement is verified by computer simulation using AMESim software.
Assuntos
Desenho Assistido por Computador , Fontes de Energia Elétrica , Transferência de Energia , Retroalimentação , Modelos Teóricos , Reologia/instrumentação , Transdutores , Simulação por Computador , Desenho de Equipamento , Análise de Falha de EquipamentoRESUMO
A robust control scheme is proposed for the position control of the electrohydrostatic actuator (EHA) when considering hardware saturation, load disturbance, and lumped system uncertainties and nonlinearities. To reduce overshoot due to a saturation of electric motor and to realize robustness against load disturbance and lumped system uncertainties such as varying parameters and modeling error, this paper proposes an adaptive antiwindup PID sliding mode scheme as a robust position controller for the EHA system. An optimal PID controller and an optimal anti-windup PID controller are also designed to compare control performance. An EHA prototype is developed, carrying out system modeling and parameter identification in designing the position controller. The simply identified linear model serves as the basis for the design of the position controllers, while the robustness of the control systems is compared by experiments. The adaptive anti-windup PID sliding mode controller has been found to have the desired performance and become robust against hardware saturation, load disturbance, and lumped system uncertainties and nonlinearities.
Assuntos
Pressão Hidrostática , Desenho de Equipamento , Modelos TeóricosRESUMO
Biosurfactants have found widespread use across multiple industrial fields, including medicine, food, cosmetics, detergents, pulp, and paper, as well as the degradation of oil and fat. The culture broth of Aureobasidium pullulans A11231-1-58 isolated from flowers of Chrysanthemum boreale Makino exhibited potent surfactant activity. Surfactant activity-guided fractionation led to the isolation of three new biosurfactants, pullusurfactins AâC (1â3). Their chemical structures were established through the use of spectroscopic techniques, predominantly 1D and 2D NMR, in conjunction with mass measurements. We evaluated the surface tension activities of isolated compounds. At 1.0 mg l-1, these compounds showed high degrees of surfactant activity (31.15 dyne/cm, 33.75 dyne/cm, and 33.83 dyne/cm, respectively).
Assuntos
Chrysanthemum , Chrysanthemum/química , Chrysanthemum/metabolismo , Tensoativos/químicaRESUMO
Candidate strains that contribute to drought resistance in plants have been previously screened using approximately 500 plant growth-promoting rhizobacteria (PGPR) obtained from Gotjawal, South Korea, to further understand PGPR associated with plant drought tolerance. In this study, a selected PGPR candidate, Flavobacterium sp. strain GJW24, was employed to enhance plant drought tolerance. GJW24 application to Arabidopsis increased its survival rate under drought stress and enhanced stomatal closure. Furthermore, GJW24 promoted Arabidopsis survival under salt stress, which is highly associated with drought stress. GJW24 ameliorated the drought/salt tolerance of Brassica as well as Arabidopsis, indicating that the drought-resistance characteristics of GJW24 could be applied to various plant species. Transcriptome sequencing revealed that GJW24 upregulated a large portion of drought- and drought-related stress-inducible genes in Arabidopsis. Moreover, Gene Ontology analysis revealed that GJW24-upregulated genes were highly related to the categories involved in root system architecture and development, which are connected to amelioration of plant drought resistance. The hyper-induction of many drought/salt-responsive genes by GJW24 in Arabidopsis and Brassica demonstrated that the drought/salt stress tolerance conferred by GJW24 might be achieved, at least in part, through regulating the expression of the corresponding genes. This study suggests that GJW24 can be utilized as a microbial agent to offset the detrimental effects of drought stress in plants.
RESUMO
Biosurfactants reduce surface and interfacial tension due to their amphiphilic properties and are an eco-friendly alternative for chemical surfactants. In this study, a new yeast strain JAF-11 that produces a biosurfactant was selected using drop collapse method, and the properties of the extracts were investigated. The nucleotide sequences of the strain were compared with closely related strains and identified based on the D1/D2 domain of the large subunit ribosomal DNA (LSU) and internal transcribed spacer (ITS) regions. Neodothiora populina CPC 39399T, the closest species with strain JAF-11, showed a sequence similarity of 97.75% for LSU and 94.27% for ITS, respectively. The result suggests that the strain JAF-11 represents a distinct species that cannot be assigned to any existing genus or species in the family Dothideaceae. Strain JAF-11 produced a biosurfactant reducing the surface tension of water from 72 mN/m to 34.5 mN/m on the sixth day of culture and the result of measuring the critical micelle concentration (CMC) by extracting the crude biosurfactant was found to be 24 mg/l. The molecular weight 502 of the purified biosurfactant was confirmed by measuring the fast atom bombardment mass spectrum. The chemical structure was analyzed by measuring 1H nuclear magnetic resonance (NMR), 13C NMR, and two-dimensional NMRs of the compound. The molecular formula was C26H46O9, and it was composed of one octanoyl group and two hexanoyl groups to myo-inositol moiety. The new biosurfactant is the first report of a compound produced by a new yeast strain, JAF-11.
Assuntos
Ascomicetos , Leveduras , Filogenia , Micelas , Tensão Superficial , TensoativosRESUMO
Biosurfactants have been widely used in various industrial fields including medicine, food, cosmetics, detergent, pulp and paper, and oil and fat degradation. The culture broth of Aureobasidium pullulans A11211-4-57 using glucose as carbon source exhibited potent surfactant activity. The culture broth was separated by column chromatographies using ODS, silica gel, and Sephadex LH-20 resins, consecutively, to provide two biosurfactants. Based on mass and NMR measurements, their structures were determined as myo-inositol lipids and named pullusurfactans F and G. These compounds showed a high degree of activity, with 27.25 mN/m and 24.07 mN/m, respectively, at 1.0 mg l-1, which is useful for washing and cleaning agents.
Assuntos
Ascomicetos , Erigeron , Ascomicetos/metabolismo , Aureobasidium , Tensoativos/químicaRESUMO
Soil is a primary source of water and inorganic nutrients vital for plant growth. In particular, the rhizosphere, a microecological region around the plant roots, is enriched with root exudates that enable beneficial microbial communities to form. Plant growth-promoting rhizobacteria (PGPR) are rhizosphere bacteria that contribute to the improvement of plant growth through diverse physiological mechanisms. Identifying PGPR is beneficial for agriculture because their use can effectively increase the productivity of plants without the harmful side effects of chemical fertilizers. To further enrich the pool of PGPR that contribute to abiotic stress resistance in plants, we screened roughly 491 bacteria that had previously been isolated in soil from Gotjawal in Jeju island, South Korea. Among several candidates, the application of Bacillus subtilis strain GOT9, led to the enhancement of drought and salt stress tolerance in Arabidopsis. In agreement with the increased stress tolerance phenotypes, its application resulted in increases in the transcripts of various drought stress- and salt stress-inducible genes in the absence or presence of the stresses. Furthermore, the treatment resulted in improved lateral root growth and development in Arabidopsis. GOT9 also led to enhanced tolerance against drought and salt stresses and to upregulation of drought-inducible genes in Brassica, a closely related crop to Arabidopsis. Taken together, these results show that GOT9 could be utilized as a biotic resource that effectively minimizes damage to plants from environmental stresses.
Assuntos
Arabidopsis , Bacillus subtilis , Brassica , Secas , Interações entre Hospedeiro e Microrganismos , Estresse Fisiológico , Arabidopsis/microbiologia , Bacillus subtilis/fisiologia , Brassica/microbiologia , Interações entre Hospedeiro e Microrganismos/fisiologia , Desenvolvimento VegetalRESUMO
Using a high-throughput metagenomic approach, we evaluated nifH-harboring bacterial communities and their assembly in the Gotjawal forest, which was naturally formed on basalt rocks with thin layer of soil. Significant differences in soil properties and community structure were observed in comparison with similar communities in various habitats, including other lava-formed forests (on Jeju Island and in Hawaii) and in regions with high humidity (Florida) or low temperatures (Alaska). nifH-harboring bacterial communities were found to assemble along gradients of environmental factors, particularly cation-exchange capacity. Unlike in other regions, in the Gotjawal forest, Paenibacillus and Clostridium, which belong to the phylum Firmicutes, were present in significantly higher proportion than in other regions. Network analysis suggested that much fewer co-occurrence relationships occurred in the Gotjawal forest than in other lava-formed forests. Our results indicate that the unique nifH-harboring bacterial community and its assembly in the Gotjawal forest are due to its distinctive soil properties, which has implications for microbial interactions and functional potentials.