RESUMO
The relationship between the frequency of loiasis objective symptoms and microfilaraemic or amicrofilaraemic infection was assessed in 1148 exposed patients also infected, or not, with Mansonella perstans. Filarial infections were detected by direct microscopy, leucoconcentration and serology, with prevalence values of 39.5% Loa loa, 5.6% M. perstans and 3.4% co-infection with both filarial species. Amicrofilaraemic or occult loiasis (OL) predominated among L. loa-infected individuals, with a prevalence of 58.2%. Hypermicrofilaraemia (>8000 microfilariae (mf)/ml) was found in 18.4% of L. loa microfilaraemic patients, with 25.7% of them harbouring more than 30,000 mf/ml. Up to 34% of patients with OL showed evidence of Calabar swelling, compared with 26.3% of microfilaraemic patients (P= 0.03). Overall 5.3% of patients presented with adult worm migration across the eye, representing 16.3% of microfilaraemic individuals and 11.4% of amicrofilaraemic patients (P= 0.13). This symptom was similarly found in patients with more than 30,000 mf/ml (22%), those with microfilaraemia between 8 and 30,000 mf/ml (15.4%) and also in individuals with low or without microfilaraemia (16.1%) (P= 0.7). Five (14.3%) hypermicrofilaraemic patients did not present any L. loa-specific objective symptoms, as well as all the patients with single M. perstans infection. The presence of adult eye worm migration as a strong predictor of high microfilaraemia density would obscure the real burden of L. loa hypermicrofilaraemia in exposed individuals. For epidemiological purposes and control strategies, the mapping of L. loa in endemic areas should also take into account the group of patients with occult loiasis.
Assuntos
Coinfecção/patologia , Loa/isolamento & purificação , Loíase/patologia , Mansonella/isolamento & purificação , Mansonelose/patologia , Animais , Coinfecção/epidemiologia , Coinfecção/parasitologia , Gabão/epidemiologia , Humanos , Contagem de Leucócitos , Loíase/epidemiologia , Loíase/parasitologia , Mansonelose/epidemiologia , Mansonelose/parasitologia , Microscopia , Carga Parasitária , Parasitemia , Prevalência , Testes SorológicosRESUMO
To know if alkaline phosphatase (AP) from schistosomes other than Schistosoma mansoni can be used as diagnostic marker for schistosomiasis in alkaline phosphatase immunocapture assay (APIA), we comparatively tested n-butanol extracts of adult worm membranes from a Venezuelan (JL) strain of S. mansoni (Ven/AWBE/Sm); a Cameroonian (EDEN) strain of Schistosoma intercalatum (Cam/AWBE/Si) and a Yemeni strain of Schistosoma haematobium (Yem/AWBE/Sh). APIA was evaluated with sera of patients from Venezuela, Senegal, and Gabon infected with S. mansoni, from Gabon infected with S. intercalatum or S. haematobium, from Chine infected with Schistosoma japonicum and from Cambodian patients infected with Schistosoma mekongi. Results indicate that 92.5% (37/40) of Venezuela sera, 75% (15/20) of Senegal sera, 39.5% (17/43) of S. haematobium sera, and 19.2% (5/26) S. intercalatum sera were APIA-positive with the Ven/AWBE/Sm preparation. APIA with the Cam/AWBE/Si preparation showed that 53.8% of S. intercalatum-positive sera had anti-AP antibodies, and 51.2% S. haematobium-positive sera cross-immunocapturing the S. intercalatum AP. APIA performed with Yem/AWBE/Sh showed that 55.8% S. haematobium sera were positive. Only two out of nine S. japonicum sera were APIA-positive with the Ven/AWBE/Sm and Cam/AWBE/Si, and no reaction was observed with Cambodian S. mekongi-positive sera. AP activity was shown to be present in all the schistosome species/strains studied. The use of APIA as a tool to explore the APs antigenicity and the presence of Schistosoma sp. infections through the detection of anti-Schistosoma sp. AP antibodies in a host, allowed us to demonstrate the antigenicity of APs of S. mansoni, S. intercalatum, and S. haematobium.
Assuntos
Fosfatase Alcalina/imunologia , Schistosoma/enzimologia , Esquistossomose/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Camboja , Feminino , Gabão , Humanos , Masculino , Schistosoma/classificação , Schistosoma/imunologia , Schistosoma haematobium/enzimologia , Schistosoma haematobium/imunologia , Schistosoma japonicum/enzimologia , Schistosoma japonicum/imunologia , Schistosoma mansoni/enzimologia , Schistosoma mansoni/imunologia , Esquistossomose/diagnóstico , Senegal , VenezuelaRESUMO
The aim of this study was to test the hypothesis of the presence of hybrids between Schistosoma guineensis and S. haematobium in the Estuaire province (Western Gabon). Egg morphometry and single-stranded conformational polymorphism (SSCP) analysis on adult worms were used in order to characterize the schistosome populations of two sites. The morphology of the eggs showed three morphotypes: S. haematobium, S. guineensis and intermediate morphotypes, but the eggs of the morphotype S. guineensis were smaller compared to the values found in the literature. Furthermore, the SSCP analysis of the adult schistosomes showed that all the patterns corresponded to that of S. haematobium and gave evidence that hybrids were absent from our samples.
Assuntos
Óvulo/química , Schistosoma/genética , Esquistossomose/parasitologia , Animais , DNA de Helmintos/genética , DNA Intergênico/genética , Fezes/parasitologia , Gabão , Humanos , Polimorfismo Conformacional de Fita Simples , Schistosoma/química , Schistosoma/crescimento & desenvolvimento , Schistosoma/isolamento & purificação , Caramujos/parasitologia , Urina/parasitologiaRESUMO
Aedes albopictus were collected in the French military camp of Libreville, Estuaire Province, Gabon, from January to March 2007 by human landing catches during an entomological evaluation of malaria transmission. Inspection of potential larval habitats within and outside the camp showed that Ae. albopictus was found only in artificial containers (discarded tires and small water containers). Associated species of mosquito larvae were Ae. aegypti (L.) and Culex quinquefasciatus. At the same time, Ae. albopictus adults and larvae were also collected from discarded tires in Tcheungue near Port Gentil, Ogoue Maritime Province. Ae. albopictus seems to be established in this part of Gabon's littoral. Further studies are necessary to investigate the extension of Ae. albopictus establishment throughout the country.
Assuntos
Aedes/classificação , Aedes/fisiologia , Animais , Demografia , Feminino , Gabão , Larva , PupaRESUMO
BACKGROUND: Hyperlactatemia is an important and common complication of severe malaria. We investigated changes in fluid compartment volumes in patients with severe malaria and control patients with the use of bioimpedence analysis. METHODS: We estimated extracellular water and total body water volumes in a total of 180 children: 56 with severe malaria, 94 with moderate malaria, 24 with respiratory tract infection, and 6 with severe diarrhea. RESULTS: There was a mean (+/-SD) decrease in total body water volume of 17+/-24 mL/kg (or 3% of total body water volume) in patients with severe malaria. This compares with a mean (+/-SD) decrease in total body water volume of 33+/-28 mL/kg (or 6% of total body water volume) in patients with severe diarrhea. There was no increase in extracellular water volume in patients with severe malaria, suggesting no significant intravascular volume depletion in patients with severe malaria. There was no relationship between lactatemia and any changes in fluid compartment volumes. CONCLUSIONS: The changes in fluid volumes that were observed are unlikely to be of physiological significance in the pathophysiology of severe malaria.
Assuntos
Acidose Láctica/etiologia , Desidratação/complicações , Malária Falciparum/complicações , Antimaláricos/uso terapêutico , Criança , Pré-Escolar , Diarreia/complicações , Feminino , Gabão , Humanos , Lactente , Malária Falciparum/tratamento farmacológico , Masculino , Quinina/uso terapêutico , Infecções Respiratórias/complicaçõesRESUMO
Metabolic complications of malaria are increasingly recognized as contributing to severe and fatal malaria. Disorders of carbohydrate metabolism, including hypoglycaemia and lactic acidosis, are amongst the most important markers of disease severity both in adults and children infected with Plasmodium falciparum. Amino acid and lipid metabolism are also altered by malaria. In adults, hypoglycaemia is associated with increased glucose turnover and quinine-induced hyperinsulinaemia, which causes increased peripheral uptake of glucose. Hypoglycaemia in children results from a combination of decreased production and/or increased peripheral uptake of glucose, due to increased anaerobic glycolysis. Patients with severe malaria should be monitored frequently for hypoglycaemia and treated rapidly with intravenous glucose if hypoglycaemia is detected. The most common aetiology of hyperlactataemia in severe malaria is probably increased anaerobic glucose metabolism, caused by generalized microvascular sequestration of parasitized erythrocytes that reduces blood flow to tissues. Several potential treatments for hyperlactataemia have been investigated, but their effect on mortality from severe malaria has not been determined.
Assuntos
Malária Falciparum/complicações , Malária Falciparum/metabolismo , Acidose Láctica/etiologia , Aminoácidos/metabolismo , Permeabilidade Capilar , Humanos , Hipoglicemia/etiologia , Lactatos/sangue , Metabolismo dos LipídeosRESUMO
The African Programme for Onchocerciasis Control (APOC) has implemented a series of surveys aimed at evaluating the long-term impact of its activities. The region of Lastourville (Gabon) is one of the selected sites for this study. A total of 886 persons was examined for skin lesions, and 459 out of them participated in detailed ocular examinations. Blackflies were collected during one year and dissected. Although the focus was found to be hypoendemic (prevalence of nodules: 7.7%), the frequency of onchodermatitis was relatively high. The lesions of the anterior segment of the eye were rare, but the prevalence of optic nerve disease, and of choroido-retinal lesions reached 5.2 and 2.7%, respectively. The annual transmission potential (2,171 infective larvae per man) was high, when compared with the results recorded in the human population. This may be due to the presence of Onchocerca spp. of animal origin in the blackflies. These results indicate that in the area of Lastourville, though regarded as the main focus of onchocerciasis in Gabon, the disease is relatively mild.
Assuntos
Oncocercose/epidemiologia , Adolescente , Adulto , Idoso , Animais , Criança , Pré-Escolar , Feminino , Gabão/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Oncocercose/prevenção & controle , Densidade Demográfica , Avaliação de Programas e Projetos de Saúde , SimuliidaeRESUMO
The purpose of this study was to determine the frequencies of opportunistic diseases among AIDS patients at the Jeanne Ebori Foundation (JEF) in Libreville, Gabon. A total 6313 file of patients treated in the internal medicine unit between 1994 and 1998 were analyzed. Findings showed that the main diseases related to AIDS classified according to seroprevalence were as follows: purigo (100%), cerebral toxoplasmosis (100%), oral candidiaisis (88%), bacteremia (87.8%), shingles (84.6%), minor salmonelosis (72%), and tuberclosis. The main diagnoses unrelated to AIDS at the JEF according to seroprevalene were typhoid (9.4%), common pneumonia (28%), bacterial meningitis (26.3%, hepatitis B (20.0%), and malaria (14%). In addition to these diseases there were nine cases of Kaposi's sarcoma, four cases of isosporosis, two cases of cryptococcosis, two cases of herpes Varicella, one case of cryptosporidiosis, and one case of isosporosis. The incidence of opportunistic disease was high in our study and must be taken in drug procurement.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Adolescente , Adulto , Feminino , Gabão/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos RetrospectivosRESUMO
BACKGROUND: Six years after the implementation of intermittent preventive treatment in pregnancy (IPTp) with sulfadoxine-pyrimethamine (SP) in Gabon, its impact on placental malaria and pregnancy outcomes remains unknown. METHODS: Age, gestational data, use of IPTp-SP and birth weight were recorded during a hospital-based cross-sectional survey performed in 2011 in 387 women at the end of pregnancy. RESULTS: Malaria prevalence was 6.7 and 5.3% in peripheral and placental blood respectively. Overall, 59.0% women took at least two IPTp-SP doses which was associated with 50% reduction of Plasmodium; (P.) falciparum infection in primigravidae. Previous malaria treatment was a risk factor for peripheral P. falciparum infection, while uptake of IPTp-SP was associated with reduced parasitaemia. Anaemia prevalence was 38.0%, low birth weight and prematurity rates were 6.0 and 12.0% respectively. Young age was associated with a higher frequency of malaria, anaemia, low birth weight and preterm delivery (p<0.01). Birth weight significantly rose with increasing age (p<0.01), parity (p=0.03) and number of SP doses (p=0.03). A birth weight reduction of 230 g in case of peripheral parasitaemia (p=0.02) and of 210 g with placental parasitaemia (p=0.13) was observed. CONCLUSIONS: Microscopic P. falciparum prevalence during pregnancy significantly declined between 2005 and 2011, following IPTp-SP implementation in Gabon. Young women and paucigravidae remain the most susceptible to malaria and associated outcomes.
Assuntos
Antimaláricos/uso terapêutico , Malária Falciparum/prevenção & controle , Plasmodium falciparum , Complicações Parasitárias na Gravidez/prevenção & controle , Resultado da Gravidez , Pirimetamina/uso terapêutico , Sulfadoxina/uso terapêutico , População Urbana , Adulto , Anemia/complicações , Anemia/epidemiologia , Antimaláricos/administração & dosagem , Peso ao Nascer , Cidades , Estudos Transversais , Combinação de Medicamentos , Feminino , Gabão , Número de Gestações , Humanos , Recém-Nascido de Baixo Peso , Recém-Nascido , Recém-Nascido Prematuro , Malária Falciparum/epidemiologia , Malária Falciparum/parasitologia , Parasitemia/epidemiologia , Parasitemia/parasitologia , Parasitemia/prevenção & controle , Placenta , Gravidez , Complicações Parasitárias na Gravidez/epidemiologia , Complicações Parasitárias na Gravidez/parasitologia , Prevalência , Pirimetamina/administração & dosagem , Sulfadoxina/administração & dosagem , Adulto JovemRESUMO
Giardiasis and cryptosporidiosis are now recognized as neglected tropical parasitic diseases. The risk of their dissemination in developing countries, such as Gabon, is increasing, due to urban crowding and poor sanitation. Accurate, simple and rapid diagnosis tools are thus necessary for the estimation of their real burden. The aim of this study was to evaluate the performances of the ImmunocardSTAT®Crypto/Giardia Rapid Assay test for the detection of Cryptosporidium (C.) spp. and Giardia (G.) duodenalis in children living in Libreville, Gabon. Stool samples of 173 healthy children were screened by routine microscopic using the merthiolate iodine formol concentration technique for Giardia, the modified Ziehl Neelsen (ZN) staining for Cryptosporidium and the ImmunocardSTAT® Crypto/Giardia RDT for the detection of Giardia and Cryptosporidium parasite forms and antigens respectively. G. duodenalis was detected with microscopy and the ImmunocardSTAT® Crypto/Giardia in 27 (15.6 %) and 22 (13.3 %) fecal samples respectively. C. spp. oocysts were found in 18 (10.4 %) ones, whereas only one sample was positive with the immunochromatographic assay. When microscopic examination was considered as the reference method, sensitivity and specificity of the ImmunocardSTAT® Crypto/Giardia Rapid Assay were found to be 63.0 %, 96.6 and 5.5 %, 99.3 % for G. duodenalis and C. spp. respectively. The prevalence of G. duodenalis and C. spp. carriage is high in children from Libreville. A low sensitivity of the ImmunocardSTAT® Crypto/Giardia for the detection of both parasites is observed. It is thus inappropriate as a diagnostic tool for detecting asymptomatic carriers.
RESUMO
The purpose of this report is to describe a case of pulmonary schistosomiasis treated at la Fondation Jeanne Ebori in Libreville, Gabon. This case is exceptional due to the rarity of the disease and the schistosomiasis agent involved. The patient was a 47-year-old woman who presented with recurrent right-sided pneumonia 6 months after initial hospitalization and nonspecific antimicrobial therapy. Her general status was altered by hyperthermia, right chest pain, and repetitive bouts of hemoptysia. Because etiological diagnosis could not be achieved and clinical condition was deteriorating, the decision was taken to perform exploratory thoracotomy. Based on operative findings, medial and lower lobectomy was performed. Histological examination demonstrated granulomatous inflammatory lesions due to bilharziosis. Infection was attributed to Schistosoma haematobium that is the most common agent in pulmonary localizations. However Ziehl coloration raised the possibility of involvement of Schistosoma intercalatum that has never been observed in the lung and of a hybrid species. Natural hybridization between S. haematobium and S. intercalatum and the presence of active transmission of the Schistosoma hybrid has been suspected in Gabon.
Assuntos
Pneumopatias/diagnóstico , Pneumopatias/patologia , Pneumopatias/parasitologia , Schistosoma haematobium/isolamento & purificação , Schistosoma haematobium/patogenicidade , Esquistossomose Urinária/diagnóstico , Esquistossomose Urinária/patologia , Animais , Diagnóstico Diferencial , Gabão , Granuloma/etiologia , Humanos , Pneumopatias/complicações , Pneumopatias/cirurgia , Pneumonectomia , Pneumonia/etiologia , Esquistossomose Urinária/complicações , Esquistossomose Urinária/cirurgia , ToracotomiaRESUMO
PIP: The protective role of breastfeeding against Giardia lamblia, a protozoan parasite endemic in Central Africa, was assessed through an analysis of stool samples from 400 infants aged 1 week-24 months in Libreville. Stool samples were collected during both the rainy and dry seasons in 1985 and 1986. At the time of stool collection, 237 infants were breastfed and 163 were bottle-fed (the mean weaning age among the 123 infants in the latter group who had at 1 point been breastfed was 8.2 months). The percentage of infants infected with cysts or trophozoites of Giardia was 1.2% in the exclusively breastfed group, 1.32% among infants who were partially breastfed (at least 2 feedings/day) and 14.7% in the bottle-fed group. The same pattern persisted when the data were grouped by age. In the 0-5 month age group, Giardia infection was found in 2.2% of the breastfed infants compared with 9.1% of the bottle-fed infants. In the 6-11 month age group, these percentages were 0 and 11.4%, respectively, while they were 2.2% and 17.6%, respectively, in the 12-23-month age group. There were no significant differences in the rate of giardiasis infection in the mothers of breastfed, partially breastfed, and bottle-fed infants in this study. These findings confirm an in vivo protective effect of human milk in Giardia infection. Of concern is the declining rate of breastfeeding in African cities, which can be expected to contribute to raising the rates of early symptomatic giardiasis and consequently the risk of malnutrition.^ieng
Assuntos
Aleitamento Materno , Giardíase/prevenção & controle , África , Pré-Escolar , Feminino , Giardíase/epidemiologia , Humanos , Lactente , Recém-Nascido , População UrbanaRESUMO
In examining skin snips from 1,830 Gabonese 32 were found to have microfilariae indistinguishable from Microfilaria rodhaini. The microfilaria is 300.2 microns long and 2.2 microns wide. Specific clinical signs were not identified, as the same person often was infected by other filaria.
Assuntos
Filariose/epidemiologia , Dermatopatias Parasitárias/epidemiologia , Adulto , Idoso , Animais , Criança , Feminino , Gabão , Humanos , Masculino , Microfilárias/isolamento & purificação , Pessoa de Meia-Idade , Pele/parasitologiaRESUMO
Immunologic analyses of sera from 47 selected individuals living in a mixed filariasis transmission zone in Gabon were carried out. Onchocerca volvulus, Loa loa, Mansonella streptocerca, and M. perstans are transmitted in this region. Based on parasitologic findings and age, the 47 individuals were stratified into four groups: microfilaria negative (Mf-) children (3-15 years old), Mf- adults (> 15 years old), microfilaria positive (Mf+) children and Mf+ adults. For descriptive purposes, the term microfilaria positive refers to individuals with skin and blood microfilariae. Antifilarial antibody titers were determined using an enzyme-linked immunosorbent assay with Dipetalonema viteae antigens. In general, children had higher titers of IgG antibodies than adults. For the IgG1, IgG2, and IgG3 subclass responses, both age and microfilarial status appeared to be important variables since Mf- children consistently had the highest titers whereas Mf- adults had the lowest titers. For the IgG4 antifilarial response, only the microfilarial status was an important variable. All Mf+ individuals had significantly higher levels of IgG4 antibody than Mf- individuals. Pooled sera of Mf- and Mf+ individuals reacted with similar O. volvulus antigens on Western blots. Control sera of individuals who did not reside in the study area, but who had single infections with L. loa or M. perstans, did not react with any O. volvulus antigens.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Filariose/imunologia , Filarioidea/imunologia , Imunoglobulina G/sangue , Dermatopatias Parasitárias/imunologia , Adolescente , Adulto , Fatores Etários , Idoso , Animais , Criança , Pré-Escolar , Dipetalonema/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Filariose/sangue , Filariose/parasitologia , Filarioidea/isolamento & purificação , Gabão , Humanos , Masculino , Microfilárias/imunologia , Microfilárias/isolamento & purificação , Pessoa de Meia-Idade , Onchocerca volvulus/imunologia , Pele/parasitologia , Dermatopatias Parasitárias/parasitologiaRESUMO
Serologic markers of hepatitis B virus (HBV), including HBV DNA, and of hepatitis delta virus were measured in three villages in Gabon. Of 303 subjects studied, 19% were carriers of hepatitis B surface antigen (HBsAg); 8.5% of these had anti-delta antibodies. No difference among the three villages was observed. All HBV DNA carriers were children less than 11 years of age. In the 2-9-year-old group, 71% of the HBsAg-positive children tested were HBV DNA carriers. These results indicate that HBV prevalence is high in Gabon and emphasize the importance of horizontal transmission of HBV in Africa. Antibodies to hepatitis C virus, assessed in one of the three villages, were found with a prevalence of 35% with a second generation enzyme-linked immunosorbent assay (ELISA) and 24% with a third generation ELISA. None of 35 subjects tested for antibodies to hepatitis E virus was positive.
Assuntos
Hepatite B/epidemiologia , Hepatite C/epidemiologia , Hepatite D/epidemiologia , Hepatite E/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Criança , Pré-Escolar , DNA Viral/sangue , Feminino , Gabão/epidemiologia , Anticorpos Anti-Hepatite/sangue , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/genética , Vírus da Hepatite B/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , População RuralRESUMO
Two clinical studies were carried out in Gabon, Africa to evaluate the efficacy, safety, and tolerability of ivermectin in the treatment of patients with Loa loa infection. In the first study, 35 patients received single oral doses of ivermectin, 5-200 mcg/kg body weight. Blood microfilariae levels did not decrease after a single oral 5, 10, 30, or 50 mcg/kg dose of ivermectin, but levels did decrease after doses of 100, 150, and 200 mcg/kg. The most efficacious dose was 200 mcg/kg; mean blood microfilariae levels decreased to 12% of mean pretreatment values by day 15 and remained decreased for 28 days. A second study evaluated the safety and efficacy of ivermectin in patients with multifilarial infections. All 17 patients had concomitant Loa loa and O. volvulus infection. M. perstans affected 5 of the patients. Sixteen patients also had infections due to intestinal nematodes. The patients each received single oral doses of 200 mcg/kg ivermectin. Ten days later, the mean Loa loa blood microfilariae level had decreased to 20% of the mean pretreatment level. O. volvulus dermal microfilariae densities were reduced to 2% of the pretreatment levels. A minimal increase in blood microfilaria levels was observed on day 28. In contrast, dermal microfilariae levels remained near zero for the duration of the study. Intestinal infection due to Ascaris was eradicated in all of the affected patients by day 23; efficacy against Trichuris and hookworm infections, however, was poor. All patients tolerated ivermectin well including those with multiple infections.
Assuntos
Filariose/tratamento farmacológico , Ivermectina/uso terapêutico , Loíase/tratamento farmacológico , Mansonelose/tratamento farmacológico , Oncocercose/tratamento farmacológico , Adulto , Idoso , Animais , Humanos , Ivermectina/efeitos adversos , Loa/crescimento & desenvolvimento , Loíase/complicações , Masculino , Mansonella/crescimento & desenvolvimento , Mansonelose/complicações , Microfilárias/crescimento & desenvolvimento , Pessoa de Meia-Idade , Onchocerca/crescimento & desenvolvimento , Oncocercose/complicaçõesRESUMO
Hydrogen breath tests were performed in Gabon (Central Africa) after a loading dose of lactose in 67 well-nourished African children (50 with intestinal parasites and 17 unparasitized) and in 18 unparasitized young adults. All had normal nutritional status, and none had diarrhea or digestive symptoms. Parasites that were found included Ascaris lumbricoides in 76% of the parasitized children, Trichuris trichiura in 58%, Giardia in 24%, Entamoeba histolytica in 20%, Schistosoma intercalatum in 16%, and Necator Americanus in 14%. A similar proportion of parasitized (64%) or unparasitized (62.8%) subjects were lactose malabsorbers. Giardia infection was associated with a higher, but not significantly different, proportion of lactose intolerance (10 of 12, 83.3%). The presence of infection with A. lumbricoides or T. trichiura did not increase the percentage of lactose malabsorption. These data indicate that a decrease of lactase activity in well-nourished African children is not related to the presence or the importance of Ascaris or other intestinal parasites if the nutritional status is normal.
Assuntos
Enteropatias Parasitárias/metabolismo , Intolerância à Lactose/parasitologia , Animais , Ascaríase/metabolismo , Ascaris/isolamento & purificação , Testes Respiratórios , Criança , Disenteria Amebiana/metabolismo , Entamoeba histolytica/isolamento & purificação , Gabão , Giardia lamblia/isolamento & purificação , Giardíase/metabolismo , Humanos , Hidrogênio , Enteropatias Parasitárias/parasitologia , Necator/isolamento & purificação , Necatoríase/metabolismo , Contagem de Ovos de Parasitas , Schistosoma/isolamento & purificação , Esquistossomose mansoni/metabolismo , Strongyloides/isolamento & purificação , Tricuríase/metabolismo , Trichuris/isolamento & purificação , beta-Galactosidase/deficiênciaRESUMO
Rapid diagnosis and accurate quantification of Plasmodium falciparum parasitemia are important for the management of malaria. The assessment of disease severity also depends on evaluation of metabolic indexes such as blood glucose and lactate concentrations. Here we describe an accurate and rapid alternative to conventional thick film examination (Lambaréné method). We also assess near-patient methods for measuring blood glucose (OneTouch) and lactate (Accusport). The accuracy of the Lambaréné method is similar to that of thin films. Results from the OneTouch glucose meter also are in good agreement with a YSI 2300 reference meter. Overall, the Accusport lactate meter agrees poorly with the YSI 2300 reference meter. However, the sensitivity and specificity to detect hyperlactatemia (blood lactate > or = 5 mmol/L) are 0.94 and 0.98, respectively.
Assuntos
Glicemia/análise , Ácido Láctico/sangue , Malária/sangue , Parasitemia/diagnóstico , Criança , Humanos , Malária/diagnóstico , Sensibilidade e EspecificidadeRESUMO
The changes in Plasmodium falciparum in four Gabonese children suffering from severe malaria and treated with pure artemether were observed in thin blood smears fixed and stained with Giemsa and examined by light microscopy. Peripheral blood samples were taken every 8 hr up to 72 hr from three children and every 3 hr up to 9 hr from the other child. The morphologic changes involved all development stages (trophozoites, schizonts, and gametocytes); they were first seen 3 hr after the start of treatment and all parasites were abnormal after 24 hr. After two days of treatment, all infected erythrocytes disappeared except for a few with necrotic trophozoites. The morphologic changes were similar to the ultrastructural changes previously described in vivo and in vitro in experimental models. They confirm the rapid effect of artemisinin derivatives on parasite clearance and clinical recovery, particularly in cases of cerebral malaria.
Assuntos
Antimaláricos/farmacologia , Artemisininas , Malária Falciparum/tratamento farmacológico , Plasmodium falciparum/efeitos dos fármacos , Sesquiterpenos/farmacologia , Animais , Antimaláricos/administração & dosagem , Antimaláricos/uso terapêutico , Artemeter , Corantes Azur , Sangue/parasitologia , Pré-Escolar , Eritrócitos/parasitologia , Gabão , Humanos , Lactente , Malária Falciparum/sangue , Microscopia/métodos , Plasmodium falciparum/crescimento & desenvolvimento , Sesquiterpenos/administração & dosagem , Sesquiterpenos/uso terapêutico , Fatores de TempoRESUMO
One hundred nine Gabonese patients infected with Loa loa microfilariae were treated with ivermectin (200 microg/kg of body weight) at the Parasitology, Mycology and Tropical Medicine Department (Faculte de Medecine et des Sciences de la Sante, Libreville, Gabon). Each was given one dose per month for six consecutive months. The peripheral blood microfilaria (mf) count before and after each dose showed an average decrease in the microfilaremia of 87.3% (short-term-single dose). An annual single-dose mass treatment with 200 microg/kg of ivermectin was sufficient to control the parasite in populations with low (< 400/ml) L. loa mf counts. One month after the sixth dose (short-term-multiple doses), the average microfilaremia rate had decreased by 99.2% compared with the initial infection (35 patients). Samples were taken from 28 patients one month after the first dose and one month after the sixth dose. The average mf count decreased by 96.4% after the first dose and by 99.6% after the sixth dose (average residual mf counts = 13.7 and 1.5 mf/ml, respectively). The mf count after the sixth dose was only 11.2% of the count after the first dose. The low mf count persisted for more than six months after the sixth treatment (long-term-multiple doses). Thus, mass treatment with multiple doses is more appropriate for areas where the blood mf count is very high. These results show that the number of the annual treatments used in mass chemotherapy with ivermectin can be adapted to each population to provide efficient protection.