Calorimetric Measurements of Biological Interactions and Their Relationships to Finite Time Thermodynamics Parameters.

A description and examination of the potential for calorimetry for use in exploring the entropy flows in biological and or reacting systems is presented. A calorimeter operation background is provided, and two case studies are investigated using a transient numerical simulation. The first case describes a single cell calorimeter containing a single phase material excited by heat generation source function such as joule heating. The second case is a reacting system. The basic observation parameter, the temperature, cannot be used to separate the entropy property changes and the rate of entropy production in the second case. The calculated transient response can be further analyzed to determine the equilibrium constant once the reaction equation and stoichiometric constants are specified which allows entropy property changes and the rate of entropy production to be determined. In a biological community, the equivalent of the reaction equation and a definition of an equilibrium constant are not available for all systems. The results for the two cases illustrate that using calorimetry measurements to identify the entropy flows in biological community activities requires further work to establish a framework similar to that chemical reacting systems that are based on an equilibrium type parameter.

A continuous flow microfluidic calorimeter: 3-D numerical modeling with aqueous reactants.

A computational analysis of the reacting flow field, species diffusion and heat transfer processes with thermal boundary layer effects in a microchannel reactor with a coflow configuration was performed. Two parallel adjacent streams of aqueous reactants flow along a wide, shallow, enclosed channel in contact with a substrate, which is affixed to a temperature controlled plate. The Fluent computational fluid dynamics package solved the Navier-Stokes, mass transport and energy equations. The energy model, including the enthalpy of reaction as a nonuniform heat source, was validated by calculating the energy balance at several control volumes in the microchannel. Analysis reveals that the temperature is nearly uniform across the channel thickness, in the direction normal to the substrate surface; hence, measurements made by sensors at or near the surface are representative of the average temperature. Additionally, modeling the channel with a glass substrate and a silicone cover shows that heat transfer is predominantly due to the glass substrate. Finally, using the numerical results, we suggest that a microcalorimeter could be based on this configuration, and that temperature sensors such as optical nanohole array sensors could have sufficient spatial resolution to determine enthalpy of reaction.