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1.
Pediatr Int ; 57(4): 597-602, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25640080

RESUMO

BACKGROUND: The reintroduction of measles-rubella combined (MR) vaccination to Japan raised concerns about adverse events as well as immunogenicity related to booster immunization in subjects with naturally acquired immunity to measles or rubella. METHODS: The time course of reactogenicity and antibody responses in recipients with pre-existing immunity to measles through natural infection was observed. Eighteen children aged 80-104 months received MR booster vaccination; 16 of them had had previous rubella vaccination. RESULTS: There were virtually no clinical reactions related to booster vaccination, and a highly significant antibody response to rubella antigen, whereas the antibody rise to measles was statistically significant but poor. CONCLUSIONS: Vaccination of individuals already immune is not harmful. Booster immunization to rubella for Japanese children is vitally important.


Assuntos
Anticorpos Anti-Idiotípicos/imunologia , Imunidade Inata , Imunoglobulina G/imunologia , Vacina contra Sarampo-Caxumba-Rubéola/farmacologia , Sarampo/prevenção & controle , Caxumba/prevenção & controle , Rubéola (Sarampo Alemão)/prevenção & controle , Criança , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Incidência , Japão/epidemiologia , Masculino , Sarampo/epidemiologia , Caxumba/epidemiologia , Estudos Retrospectivos , Rubéola (Sarampo Alemão)/epidemiologia , Instituições Acadêmicas , Vacinação/métodos
2.
J Med Virol ; 86(4): 658-65, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24395026

RESUMO

For a better understanding of the cellular immune responses to reactivated HHV 6B the lymphoproliferative response to human herpesvirus 6B (HHV 6B) antigen was measured in three consecutive specimens obtained biweekly from 22 young children and infants suffering from acute measles, and in 19 influenza patients and nine healthy control subjects. HHV 6B DNA in peripheral blood mononuclear cells (PBMCs) was detected in 18 of 22 subjects with measles, but not in the influenza patients or the healthy population. A novel reactivation profile of HHV 6B was found in patients with measles in the milder form of immunosuppression than in patients with organ transplantation. HHV 6B specific lymphoproliferation activities increased correspondingly with reactivation of HHV 6B assessed by detecting HHV 6B DNA in PBMCs in patients with measles, but no significant change in either the antibody response to HHV 6B or DNAemia occurred in serial specimens obtained either from patients with influenza or healthy subjects. This novel form of HHV 6B reactivation without antibody response was observed in patients with measles. The dynamic fluctuations in lymphoproliferative responses in measles may represent the balance between HHV 6B reactivation and its suppression by the host immune system.


Assuntos
Herpesvirus Humano 6/imunologia , Influenza Humana/imunologia , Leucócitos Mononucleares/imunologia , Linfócitos/imunologia , Sarampo/imunologia , Proliferação de Células , Pré-Escolar , DNA Viral/sangue , Feminino , Testes de Inibição da Hemaglutinação , Humanos , Tolerância Imunológica , Imunidade Celular , Lactente , Influenza Humana/virologia , Leucócitos Mononucleares/virologia , Masculino , Sarampo/virologia , Infecções por Roseolovirus/imunologia , Ativação Viral/imunologia
3.
Influenza Other Respir Viruses ; 17(1): e13053, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36086810

RESUMO

BACKGROUND: Anaphylaxis following influenza vaccination is a rare but serious problem. The underlying immune responses are not well understood. This study elucidated the IgE and IgG antibody responses in healthy children and adolescents following inactivated influenza vaccines (IIVs). METHODS: The efficacy and safety of quadrivalent IIV (QIV) and trivalent IIV (TIV) were compared in healthy subjects aged 0-18 years. Serum IIV-specific IgE, IgG, and IgG4 levels (sIgE, sIgG, and sIgG4) were measured with ImmunoCAP. Hemagglutination inhibition (HI) assay was performed for each influenza virus subtype. Sera from earlier patients who developed anaphylaxis to different IIVs were similarly tested. RESULTS: A total of 393 subjects were enrolled: 96 were 6 months-2 years old, 100 were 3-5 years old, 100 were 6-12 years old, and 97 were 13-18 years old. No anaphylaxis was observed. Generally, QIV and TIV induced similar antibody responses. IIV-sIgE levels rose significantly after vaccination in the 6 months-2 years old and 3-5 years old groups, did not change in the 6-12 years old group, and decreased in the 13-18 years old group. In contrast, the IIV-sIgG4/sIgE ratio increased significantly after vaccination in all age groups. Sensitized subjects had significantly higher HI titers and IIV-sIgG levels in the youngest age group and higher IIV-sIgG4 levels in all age groups compared with the non-sensitized. The IIV-sIgG4/sIgE ratio in five patients with anaphylaxis was significantly lower than in age-matched healthy subjects. CONCLUSION: IIVs induce IgE sensitization in healthy children but also robust IgG4 responses that may protect them from anaphylaxis.


Assuntos
Vacinas contra Influenza , Influenza Humana , Adolescente , Humanos , Criança , Pré-Escolar , Vacinas contra Influenza/efeitos adversos , Imunoglobulina G , Vacinas de Produtos Inativados , Prevalência , Anticorpos Antivirais , Testes de Inibição da Hemaglutinação , Imunoglobulina E
4.
Scand J Infect Dis ; 44(8): 605-9, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22497443

RESUMO

BACKGROUND: Young infants with influenza virus infection are frequently hospitalized, and are at risk of serious complications including death. With the emergence of pandemic influenza A/H1N1 2009, oseltamivir was approved for use in Europe and the USA, including use in infants aged < 3 months. However, few data are available regarding the safety of oseltamivir treatment for influenza in infants aged < 3 months. METHODS: The clinical data from Japanese infants aged < 3 months with laboratory-confirmed influenza virus infections, who were treated with oseltamivir between October 2009 and April 2011, were collected and analyzed. RESULTS: Forty-four infants were included in the study. The median age was 1 month (range 4 days to 2 months) and median body weight was 4.5 kg (range 2.6-7.6 kg). Thirty-eight infants (86%) had no underlying diseases. The most common presenting symptom was fever (42 infants, 95%). There were no cases of influenza-associated encephalopathy or myocarditis. The median time between the onset of influenza symptoms and initiation of oseltamivir treatment was 0 days (range 0-7 days), with treatment initiated within 1 day in 40 infants (91%). The oseltamivir dose was 1.5-2 mg/kg twice daily in 98% of infants. No serious adverse events were identified during treatment. All infants recovered completely. CONCLUSIONS: Treatment of influenza with oseltamivir 1.5-2 mg/kg twice daily may be safe in infants aged < 3 months.


Assuntos
Antivirais/efeitos adversos , Influenza Humana/tratamento farmacológico , Oseltamivir/efeitos adversos , Antivirais/uso terapêutico , Humanos , Lactente , Recém-Nascido , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Influenza Humana/virologia , Japão , Oseltamivir/uso terapêutico , Resultado do Tratamento
5.
Kansenshogaku Zasshi ; 85(2): 161-5, 2011 Mar.
Artigo em Japonês | MEDLINE | ID: mdl-21560419

RESUMO

Oka varicella vaccine was developed to confer active immunity to varicella-zoster virus (VZV) in immunocompromized and immunocompetent children. It is now used to prevent varicella in about 20 million people worldwide. Although VZV infectivion is relatively unstable compared to other viruses, cell-free virus is stabilized and lyophilized vaccine has been developed. Virus titers were evaluated in vaccine distributed to six clinics in 5 years. Yearly mean virus titers at the vaccine producer were 42,000-67,000 plaque-forming units per dose, corresponding to Oka varicella vaccine (Zostavax) used to prevent zoster and postherpetic neuralgia by Oxman et al. Virus titer was found to be stable during delivery to clinics. Virus titers of varicella vaccine were equivalent to Zostavax and vaccine delivered to clinics had enough virus titer to confer active immunity to VZV in this study.


Assuntos
Vacina contra Varicela/normas , Vacina contra Varicela/imunologia
6.
Vaccines (Basel) ; 8(2)2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32230902

RESUMO

Recombinant measles AIK-C vaccine expressing the hemagglutinin (HA) protein of influenza A/Sapporo/107/2013(H1N1pdm) (MVAIK/PdmHA) was constructed. Measles particle agglutination (PA) and influenza hemagglutinin inhibition (HI) antibodies were induced in cotton rats immunized with MVAIK/PdmHA. Cotton rats immunized with two doses of the HA split vaccine were used as positive controls, and higher HI antibodies were detected 3 weeks after the first dose. Following the challenge of A/California/07/2009(H1N1pdm), higher viral loads (107 TCID50/g) were detected in the lung homogenates of cotton rats immunized with the empty vector (MVAIK) or control groups than those immunized with MVAIK/Pdm HA (103 TCID50/g) or the group immunized with HA split vaccine (105 TCID50/g). Histopathologically, destruction of the alveolar structure, swelling of broncho-epithelial cells, and thickening of the alveolar wall with infiltration of inflammatory cells and HA antigens were detected in lung tissues obtained from non-immunized rats and those immunized with the empty vector after the challenge, but not in those immunized with the HA spilt or MVAIK/PdmHA vaccine. Lower levels of IFN-α, IL-1ß, and TNF-α mRNA, and higher levels of IFN-γ mRNA were found in the lung homogenates of the MVAIK/PdmHA group. Higher levels of IFN-γ mRNA were detected in spleen cell culture from the MVAIK/PdmHA group stimulated with UV-inactivated A/California/07/2009(H1N1pdm). In conclusion, the recombinant MVAIK vaccine expressing influenza HA protein induced protective immune responses in cotton rats.

7.
Hum Vaccin Immunother ; 14(12): 2990-2998, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30036123

RESUMO

A clinical trial of a quadrivalent split influenza vaccine was performed in the 2014/15 season. Sixty-four subjects aged 6 months to 18 years were enrolled in order to investigate the relationship between cellular and humoral immune responses. Subjects were categorized into two groups by measuring neutralizing antibodies: non-primed naïve/primed or seroconverted/non-seroconverted groups. Whole-blood cultures were stimulated with the H1N1 split antigen before immunization and one month after the first and second immunizations for subjects < 13 years and before and one month after the first dose for those ≥ 13 years in order to investigate cytokine production. Significant amounts of IL-2, IL-12, IL-13, MCP-1, MIP-1ß, and TNF-α were detected from one month after the first dose in the naïve group. In addition to these cytokines, the production of IL-1ß, IL-4, IL-6, IL-8, IL-10, IL-17, G-CSF, and IFN-γ was enhanced one month after the second dose. No significant increase was noted in the primed group, except in the production of IL-10. In seroconverted subjects, the production of IL-2, IL-4, IL-8, IL-10, G-CSF, MCP-1, TNF-α, and IFN-γ increased one month after the first dose, which was earlier than in the naïve group, whereas no significant cytokine response was noted in subjects without seroconversion. Subjects ≥ 13 years were primed and the production of G-CSF, IL-4, and IL-1ß increased in subjects with seroconversion. Whole-blood cultures were also stimulated with the H3N2 split antigen and similar cytokine profiles were obtained. Many cytokines and chemokines, including inflammatory cytokines, were produced in seroconverted, but not non-seroconverted subjects.

8.
Vaccine ; 33(45): 6099-105, 2015 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-26188254

RESUMO

Although anaphylaxis is an extremely rare vaccine-associated adverse event, it occurred in young children following administration of the 2011/12 seasonal split influenza vaccine, which contained 2-phenoxyethanol as the preservative. These children had high levels of IgE antibodies against influenza vaccine components. We herein investigated why these children were sensitized. One hundred and seventeen series of serum samples were obtained immediately before, and one month after the first and second immunizations with the HA split vaccine of 2011/12. Forty-two sequential serum samples were collected in the acute and convalescent phases (2 and 4 weeks) after natural infection with H1N1 Pdm in 2009. IgE antibodies developed following the vaccination of young children with seasonal split vaccines, whereas no significant IgE response was observed following natural infection with H1N1 Pdm 2009. The prevalence of IgE antibodies was not influenced by outbreaks of H1N1 Pdm. Repeated immunization with the HA split vaccine induced IgE sensitization against the influenza vaccine irrespective of the H1N1, H3N2, or B influenza subtypes. The reasons why anaphylaxis only occurred in recipients of the influenza vaccine containing 2-phenoxyethanol are still being investigated, and the size distribution of antigen particles may have shifted to a slightly larger size. Since the fundamental reason was IgE sensitization, current split formulation for the seasonal influenza vaccine needs to be reconsidered to prevent the induction of IgE sensitization.


Assuntos
Anafilaxia/etiologia , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Vacinas contra Influenza/imunologia , Adolescente , Criança , Pré-Escolar , Surtos de Doenças , Etilenoglicóis , Feminino , Testes de Inibição da Hemaglutinação , Humanos , Imunidade Inata , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Lactente , Recém-Nascido , Vírus da Influenza A Subtipo H1N1/imunologia , Vírus da Influenza A Subtipo H3N2/imunologia , Vírus da Influenza B/imunologia , Vacinas contra Influenza/química , Influenza Humana/prevenção & controle , Masculino , Conservantes Farmacêuticos , Estações do Ano , Vacinação , Vacinas de Produtos Inativados/efeitos adversos , Vacinas de Produtos Inativados/imunologia
9.
Hum Vaccin Immunother ; 10(3): 677-85, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24589970

RESUMO

Haemophilus influenzae type b (Hib) and 7-valent pneumococcal (PCV7) vaccines both became recommended in Japan in 2010. In this study, cytokine production was investigated in peripheral blood mononuclear cells (PBMCs) cultures stimulated with diphtheria and tetanus toxoids combined with acellular pertussis vaccine (DPT), Hib, and PCV7 separately or concurrent different combinations, all as final off-the-shelf vaccines without the individual vaccine components as controls. Higher IL-1ß levels were produced when cultures were stimulated with PCV than with DPT or Hib, and the concurrent stimulation including PCV7 enhanced the production of IL-1ß. Although Hib induced higher levels of IL-6, no significant difference was observed in IL-6 production with the concurrent stimulation. The concurrent stimulation with Hib/PCV7 and DPT/Hib/PCV7 produced higher levels of TNF-α and human G-CSF. Cytokine profiles were examined in serum samples obtained from 61 vaccine recipients with febrile reactions and 18 recipients without febrile illness within 24 h of vaccination. No significant difference was observed in cytokine levels of IL-1ß, IL-4, IL-6, IL-10, IL-12, IFN-γ, MIP-1, TNF-α, and prostaglandin E2 (PGE2) in sera between the two groups. However, significantly higher levels of human G-CSF were observed in recipients with febrile illness than in those without febrile reactions. Further investigations of the significance of elevated serum G-CSF levels are required in vaccine recipients with febrile illness.


Assuntos
Citocinas/metabolismo , Vacina contra Difteria, Tétano e Coqueluche/imunologia , Vacinas Anti-Haemophilus/imunologia , Leucócitos Mononucleares/imunologia , Vacinas Pneumocócicas/imunologia , Células Cultivadas , Criança , Pré-Escolar , Vacina contra Difteria, Tétano e Coqueluche/administração & dosagem , Vacinas Anti-Haemophilus/administração & dosagem , Vacina Pneumocócica Conjugada Heptavalente , Humanos , Lactente , Japão , Masculino , Vacinas Pneumocócicas/administração & dosagem
10.
Viral Immunol ; 27(8): 368-74, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25211638

RESUMO

The 2009 pandemic H1N1 mainly affected adolescents and children, and most of the elderly in Japan escaped clinical illness. To clarify the role of humoral immunity in the infection, the time kinetics of hemagglutination inhibition (HI), neutralization (NT), and IgG subclass antibody response directed against influenza A(H1N1)pdm2009 were analyzed in three consecutive specimens obtained from 51 young adults and children (group 1) who contracted pandemic influenza and from 74 pediatric clinic employees (group 2) inoculated with pandemic monovalent vaccine. In group 1 patients, 6 and 30 patients had lower HI and NT antibody in the acute phase respectively. Thereafter, HI and NT antibody titers increased fourfold or more in 50 patients with peak response in the third specimens obtained four weeks after the onset. IgG1 in 45 patients, IgG3 in 18 patients, and IgG4 in 29 patients showed elevated responses. Forty (54%) and 70 (95%) subjects in group 2 had positive HI and NT antibodies in the prevaccination samples, with increased antibody responses in the follow-up peaking in the second specimens. Forty of those vaccinated had increased IgG1 responses peaking in the third specimens, whereas elevated IgG3 was observed in 22 recipients with the highest level in the second samples. IgG4 did not show any increase in subjects in group 2. A few participants showed an IgG2 response in both groups. An immunologically naive population contracted influenza with apparent clinical symptoms. However, already primed subjects through subclinical infection elicited the unique pattern of IgG subclass responses by vaccination, which differed from those of naive populations.


Assuntos
Anticorpos Antivirais/sangue , Imunidade Humoral , Vírus da Influenza A Subtipo H1N1/imunologia , Vacinas contra Influenza/imunologia , Influenza Humana/imunologia , Influenza Humana/virologia , Adolescente , Adulto , Idoso , Anticorpos Neutralizantes/sangue , Criança , Pré-Escolar , Feminino , Testes de Inibição da Hemaglutinação , Humanos , Imunoglobulina G/sangue , Lactente , Vacinas contra Influenza/administração & dosagem , Japão , Masculino , Pessoa de Meia-Idade , Testes de Neutralização , Fatores de Tempo , Adulto Jovem
11.
Vaccine ; 30(44): 6338-9, 2012 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-22906723

RESUMO

Influenza HA split vaccine is widely administered to children and adults worldwide. However, its limited efficacy and weak potential to control the next influenza pandemic has recently been emphasized and has raised serious concerns. These circumstances led the Japanese Society for Vaccinology to organize this symposium to discuss the next generation of influenza vaccine which should be more efficacious and much safer. The symposium covered data assessment of the newly developed H5N1 vaccine, adjuvant development for influenza vaccine, the future threat of H5N1 influenza, measures for its control, and the recent development of a mucosal vaccine.


Assuntos
Descoberta de Drogas/tendências , Vacinas contra Influenza/imunologia , Influenza Humana/prevenção & controle , Adjuvantes Imunológicos/administração & dosagem , Administração através da Mucosa , Humanos , Virus da Influenza A Subtipo H5N1/genética , Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/genética , Tóquio
12.
Vaccine ; 30(52): 7662-6, 2012 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-23102974

RESUMO

IgG subclass antibody responses are not fully understood. Alum-adjuvanted H5N1whole virion inactivated vaccine (WIV), a genetically reassortant vaccine seed strain originating from H5N1/A/Vietnam/1194/2004 and PR-8, induced significantly stronger antibody responses in neutralizing antibodies in children. In this report, IgG subclass antibody responses were investigated, and most serum samples were positive for IgG1 antibody before immunization. A significant response (more than 4-fold increase) of IgG1 antibody was observed in 67/193 (34.7%) and that of gG4 antibodies in 42/193(21.8%). Children <4 years of age showed a significant increase in IgG subclass antibodies but those ≥4 years showed lower responses. Alum- adjuvanted H5N1WIV induced an efficient immune response in young children especially <4 years.


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Compostos de Alúmen/administração & dosagem , Anticorpos Antivirais/sangue , Imunoglobulina G/sangue , Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/imunologia , Adolescente , Fatores Etários , Criança , Pré-Escolar , Humanos , Lactente , Vacinas contra Influenza/administração & dosagem , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/imunologia
13.
Vaccine ; 30(26): 3885-90, 2012 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-22507655

RESUMO

Alum-adjuvanted H5 whole virion inactivated vaccine (WIV) was licensed for adults in Japan but induced marked febrile reactions with significantly stronger antibody responses in children. In this study, the mechanisms behind the different responses were investigated. Lymphocytes were obtained from 25 healthy subjects who were not immunized with H5 vaccine, to examine the innate immune impact of the various vaccine formulations, analyzing the cytokine production profile stimulated with alum adjuvant alone, alum-adjuvanted H5 WIIV, plain H5 WIV, and H5 split vaccine. Alum adjuvant did not induce cytokine production, but H5 split induced IFN-γ and TNF-α. H5 WIV induced IL-6, IL-17, TNF-α, MCP-1, IFN-γ, and IFN-α. An extremely low level of IL-1ß was produced in response to H5 WIV, and alum-adjuvanted H5 WIV enhanced IL-1ß production, with similar levels of other cytokines stimulated with H5 WIV. Enhanced production of cytokines induced by alum-adjuvanted H5 WIV may be related to the higher incidence of febrile reactions with stronger immune responses in children but it should be further investigated why efficient immune responses with febrile illness were observed only in young children.


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Compostos de Alúmen/administração & dosagem , Citocinas/metabolismo , Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/imunologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Criança , Pré-Escolar , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/epidemiologia , Feminino , Febre/induzido quimicamente , Humanos , Lactente , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/efeitos adversos , Japão , Masculino , Pessoa de Meia-Idade , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/efeitos adversos , Vacinas de Produtos Inativados/imunologia , Adulto Jovem
14.
J Clin Virol ; 50(1): 65-8, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21035385

RESUMO

BACKGROUND: Cytokines and chemokines induced by human herpesvirus 6 (HHV-6) infection may play an important role in the observed HHV-6-associated clinical complications. However, basic data for cytokine and chemokine synthesis in primary HHV-6 infected patient without complication is lacking. OBJECTIVE: Aim of this study was to elucidate basic kinetic data for expressions of cytokines and chemokines in patients with primary HHV-6 infection without complication. STUDY DESIGN: Twenty-six patients suffering from fever were enrolled in this study. Fourteen biomarkers were measured in 74 serially collected sera samples from 26 patients. Additionally, serum samples obtained from 14 healthy children were used for control. RESULTS: Twenty of the 26 patients were diagnosed with primary HHV-6 infection based on viral isolation and serological analysis. The mean age (P=0.1289) and proportion of males to females (P=0.9999) between the patients with and without primary HHV-6 infection were not statistically different. At the acute phase of the disease, three cytokines (IFN-γ; P=0.0046, IL-2; P=0.0366, and IL-4; P=0.0255) and one chemokine (MCP-1; P=0.0019) were significantly higher in patients with primary HHV-6 infection compared to those without infection. Interleukin-5 levels during the convalescent period were significantly higher in patients with HHV-6 infection (P=0.0205). By 1 month post-infection, cytokine and chemokine expression had returned to almost basal levels. CONCLUSION: As suggested by the previous in vitro studies, present in vivo analysis also suggests that HHV-6 has potency for induction of cytokines and chemokines.


Assuntos
Quimiocinas/sangue , Citocinas/sangue , Herpesvirus Humano 6/imunologia , Infecções por Roseolovirus/imunologia , Anticorpos Antivirais/sangue , Feminino , Perfilação da Expressão Gênica , Herpesvirus Humano 6/isolamento & purificação , Humanos , Lactente , Masculino
15.
J Med Virol ; 80(3): 517-23, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18205215

RESUMO

Clinically apparent mumps reinfection is considered extremely rare, but several cases have been suspected of reinfection in an out-patient clinic. In this study, virological examination, virus isolation, the reverse transcription loop-mediated isothermal amplification (RT-LAMP), and IgG and IgM EIA antibodies, were examined in order to identify mumps reinfection. Patients were divided into three categories; the reinfection group comprised 29 patients with a history of natural infection, the vaccine-failure group consisted of 37 patients with an immunization history, and two patients had histories of both immunization and mumps infection. Another 25 patients were enrolled as a primary infection group. Mumps virus was isolated in 5 (17%) and the genome was detected in 12 (41%) of 29 in the reinfection group. Reinfection was confirmed in 21/28, demonstrating high avidity of IgG EIA. Mumps virus was isolated in 15 (41%) and there was a higher positivity of genome amplification in 25 (68%) of 37 patients in the vaccine-failure group. Among these, 23 were confirmed as secondary vaccine failure by high avidity IgG EIA serology. In the primary infection group, the isolation rate and genome detection rate was higher in 16 (64%) and in 18 (72%) of 25 patients, respectively. There was no significant difference in virus load among the three groups but high mumps virus load was suspected in the IgM EIA-positive group based on the shorter amplification time on RT-LAMP. Mumps virus reinfection was confirmed by RT-LAMP and an IgG avidity test and was not a rare event.


Assuntos
Anticorpos Antivirais/sangue , Vírus da Caxumba/isolamento & purificação , Caxumba/diagnóstico , Técnicas de Amplificação de Ácido Nucleico/métodos , Anticorpos Antivirais/imunologia , Genoma Viral , Genótipo , Humanos , Caxumba/imunologia , Caxumba/virologia , Vacina contra Caxumba/imunologia , Vírus da Caxumba/genética , Vírus da Caxumba/imunologia , Vírus da Caxumba/fisiologia , Recidiva , Carga Viral
16.
Vaccine ; 25(14): 2742-7, 2007 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-16530894

RESUMO

To compare the incidence of aseptic meningitis associated with symptomatic natural mumps infection and in mumps vaccine recipients, we conducted a prospective comparative study. Consecutive samples of 1051 children with mumps were enrolled by 10 pediatricians and 21,465 vaccine recipients by 143 pediatric primary care practitioners, from January 1, 2000 to January 1, 2003. Parents used a daily diary to record symptoms during the period of illness (15 days) or 30-day period following immunization. Mumps infection was confirmed by virus isolation and/or detection of mumps virus genome in salivary and CSF samples. The incidence of aseptic meningitis was 13/1051 (1.24%) in patients with symptomatic natural mumps infection and was estimated to be 0.7-1.1% of overall infection in considering asymptomatic infection, and 10/21,465 (0.05%) in vaccine recipients. Although aseptic meningitis is a clear side effect of the mumps vaccine, the incidence is considerably lower than among those with symptomatic natural infection. Our results provide an informative data for consideration to resume mumps vaccine as a part of routine immunization schedule for Japanese children.


Assuntos
Meningite Asséptica/epidemiologia , Vacina contra Caxumba/efeitos adversos , Caxumba/complicações , Adolescente , Criança , Pré-Escolar , Feminino , Hospitalização , Humanos , Incidência , Lactente , Japão/epidemiologia , Masculino , Estudos Prospectivos
17.
J Med Virol ; 78(6): 792-9, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16628576

RESUMO

The time-course of cell-mediated immunity in exanthema subitum is not well documented. The lymphoproliferative response to purified human herpesvirus 6 (HHV-6) antigen and to phytohemagglutinin was measured and natural killer (NK) cell activities determined in three consecutive specimens obtained biweekly from 18 young children and infants with exanthema subitum. Virus isolation and PCR detection of virus DNA and determination of neutralization antibody to HHV-6 and -7 were also carried out. The magnitude of the HHV-6 specific lymphoproliferative response varied; however, in most cases the time course kinetics revealed a low response in the acute phase with a subsequent gradual increase. In contrast, NK cell activities were high in the acute phase and declined gradually during convalescence. The lymphoproliferative response to phytohemagglutinin did not show a consistent trend in kinetics of time; however, dynamic changes in activity were observed in patients during the acute and convalescent periods. The results suggest that NK cells play a major role in resolving acute phase infection while specific lymphocyte activity develops later. The cause of the delayed development of HHV-6 specific lymphoproliferative response is unknown. The lymphoproliferative response to phytohemagglutinin ratios implied that HHV-6 infection has some impact on host T-cell immunity during the course of exanthema subitum.


Assuntos
Exantema Súbito/imunologia , Exantema Súbito/virologia , Herpesvirus Humano 6/imunologia , Células Matadoras Naturais/metabolismo , Proliferação de Células , Feminino , Herpesvirus Humano 7/imunologia , Humanos , Imunidade Celular , Lactente , Masculino , Infecções por Roseolovirus/imunologia , Fatores de Tempo
18.
Vaccine ; 24(17): 3618-23, 2006 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-16530300

RESUMO

During the 2000/2001 influenza season in Japan, children ranging in age from 6 months to 13 years with fever exceeding 37.5 degrees C were recruited. Vaccine efficacy was evaluated by comparing the rates of pre-seasonal vaccination between groups stratified by fever severity. Seven hundred and sixty one patients (33.1%), culture positive for influenza were enrolled for analysis. The numbers of patients for A/H1N1 and A/H3N2 were insufficient for statistical analysis. For influenza B the odds ratio for vaccinated children to have a maximum fever exceeding 39.5 degrees C was 0.52 (95% CI, 0.30-0.92) Our findings suggest modest impact of influenza vaccination on limiting severity of disease symptoms.


Assuntos
Vacinas contra Influenza/imunologia , Influenza Humana/prevenção & controle , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Surtos de Doenças , Febre/prevenção & controle , Humanos , Lactente , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Vírus da Influenza B/isolamento & purificação , Influenza Humana/epidemiologia , Influenza Humana/virologia , Japão/epidemiologia , Fatores de Tempo , Vacinação , Vacinas de Produtos Inativados/imunologia
19.
Vaccine ; 22(25-26): 3404-10, 2004 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-15308365

RESUMO

Twenty-two and 37 infants and young children received two doses of influenza HA vaccine before the 2001-2002 influenza season and before the 2002-2003 season, respectively. Two or three serial specimens were obtained, before and 1 month after the first vaccination as well as 1 month after the second vaccination. Infants showed a significantly poor HI antibody rise and lymphocyte response compared with young children aged > or =12 months. Time kinetics of the lymphoproliferative responses to influenza antigen among young children varied whereas their activities in infants were typically negative before immunization and increased after vaccination. Infants responded poorly to HA influenza vaccine compared with young children.


Assuntos
Envelhecimento/imunologia , Imunidade Celular/imunologia , Vacinas contra Influenza/imunologia , Adulto , Anticorpos Antivirais/análise , Anticorpos Antivirais/biossíntese , Especificidade de Anticorpos , Pré-Escolar , Feminino , Testes de Inibição da Hemaglutinação , Humanos , Imunidade Celular/fisiologia , Esquemas de Imunização , Lactente , Japão , Cinética , Contagem de Linfócitos , Linfócitos/imunologia , Masculino
20.
J Med Virol ; 73(1): 97-104, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15042655

RESUMO

We isolated 872 strains of mumps virus from naso-pharyngeal secretions in seven different districts of Japan from January 2000 to July 2001. Among them, 57 strains were geno-typed by nucleotide sequencing in part of the hemagglutinin-neuraminidase (HN) and small hydrophobic (SH) protein regions. Four different genotypes (B, G, K, and L) of mumps virus were co-circulating in Japan and the distribution of genotypes varied in geographically different districts. Two new clusters designated as genotypes K and L had more than 7% nucleotide variation in the SH gene. Among the 57 strains, 11 were classified as B, 35 as G, three as K, and eight as L, which was mainly isolated in Tokyo. We also examined 104 stains isolated in a clinic in Mie prefecture from 1993 to 2003. Genotype B was the indigenous strain and genotype K was introduced in 1994. Genotypes B and K co-circulated in the 1990s and were replaced by genotype G in 2000. There was no significant change in neutralizing test antibody titers against genotypes B, G, K, and L using seven post-vaccination sera with Hoshino strain (genotype B) and these four genotypes had a different antigenicity from genotype A. We should continue to watch on mumps virus molecular epidemiology.


Assuntos
Vírus da Caxumba/classificação , Vírus da Caxumba/genética , Caxumba/epidemiologia , Caxumba/virologia , Sequência de Aminoácidos , Anticorpos Antivirais/sangue , Variação Antigênica , Antígenos Virais/genética , Sequência de Bases , DNA Viral/genética , Genes Virais , Genótipo , Proteína HN/genética , Humanos , Japão/epidemiologia , Epidemiologia Molecular , Dados de Sequência Molecular , Caxumba/imunologia , Vírus da Caxumba/imunologia , Vírus da Caxumba/isolamento & purificação , Testes de Neutralização , Filogenia , Homologia de Sequência de Aminoácidos , Fatores de Tempo , Proteínas Virais/genética
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