RESUMO
Optimal coding principles are implemented in many large sensory systems. They include the systematic transformation of external stimuli into a sparse and decorrelated neuronal representation, enabling a flexible readout of stimulus properties. Are these principles also applicable to size-constrained systems, which have to rely on a limited number of neurons and may only have to fulfill specific and restricted tasks? We studied this question in an insect system--the early auditory pathway of grasshoppers. Grasshoppers use genetically fixed songs to recognize mates. The first steps of neural processing of songs take place in a small three-layer feed-forward network comprising only a few dozen neurons. We analyzed the transformation of the neural code within this network. Indeed, grasshoppers create a decorrelated and sparse representation, in accordance with optimal coding theory. Whereas the neuronal input layer is best read out as a summed population, a labeled-line population code for temporal features of the song is established after only two processing steps. At this stage, information about song identity is maximal for a population decoder that preserves neuronal identity. We conclude that optimal coding principles do apply to the early auditory system of the grasshopper, despite its size constraints. The inputs, however, are not encoded in a systematic, map-like fashion as in many larger sensory systems. Already at its periphery, part of the grasshopper auditory system seems to focus on behaviorally relevant features, and is in this property more reminiscent of higher sensory areas in vertebrates.
Assuntos
Estimulação Acústica , Vias Auditivas/fisiologia , Percepção Auditiva/fisiologia , Gafanhotos/fisiologia , Animais , Comportamento Animal/fisiologia , Comportamento Sexual Animal , Vocalização AnimalAssuntos
Desenho de Fármacos , Proteína gp41 do Envelope de HIV/química , Inibidores da Fusão de HIV/química , HIV-1/metabolismo , Fusão de Membrana/efeitos dos fármacos , Peptídeos/química , Sequência de Aminoácidos , Membrana Celular , Dicroísmo Circular , Ensaio de Imunoadsorção Enzimática , Proteína gp41 do Envelope de HIV/metabolismo , Humanos , Mimetismo Molecular , Dados de Sequência MolecularRESUMO
We describe a general method for the mimicry of one face of an alpha-helix based on a terphenyl scaffold that spatially projects functionality in a manner similar to that of two turns of an alpha-helix. The synthetic scaffold reduces the flexibility and molecular weight of the mimicked protein secondary structure. We have applied this design to the development of antagonists of the alpha-helix binding protein Bcl-x(L). Using a sequential synthetic strategy, we have prepared a library of terphenyl derivatives to mimic the helical region of the Bak BH3 domain that binds Bcl-x(L). Fluorescence polarization assays were carried out to evaluate the ability of terphenyl derivatives to displace the Bcl-x(L)-bound Bak peptide. Terphenyl 14 exhibited good in vitro affinity with a K(i) value of 0.114 muM. These terphenyl derivatives were more selective at disrupting the Bcl-x(L)/Bak over the HDM2/p53 interaction, which involves binding of the N-terminal alpha-helix of p53 to HDM2. Structural studies using NMR spectroscopy and computer-aided docking simulations suggested that the helix binding area on the surface of Bcl-x(L) is the target for the synthetic ligands. Treatment of human embryonic kidney 293 (HEK293) cells with terphenyl derivatives resulted in the disruption of the binding of Bcl-x(L) to Bax in intact cells.
Assuntos
Proteínas de Membrana/química , Proteínas Proto-Oncogênicas c-bcl-2/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-bcl-2/química , Compostos de Terfenil/química , Compostos de Terfenil/farmacologia , Materiais Biomiméticos/síntese química , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Linhagem Celular , Cristalografia por Raios X , Polarização de Fluorescência , Humanos , Cinética , Proteínas de Membrana/antagonistas & inibidores , Proteínas de Membrana/metabolismo , Proteínas de Membrana/farmacologia , Modelos Moleculares , Peso Molecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Fragmentos de Peptídeos/farmacologia , Estrutura Secundária de Proteína , Proteínas Proto-Oncogênicas/química , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/farmacologia , Relação Estrutura-Atividade , Compostos de Terfenil/síntese química , Proteína Killer-Antagonista Homóloga a bcl-2 , Proteína bcl-XRESUMO
The design of synthetic agents to disrupt protein-protein interactions has received relatively little attention in recent years. In this review we describe strategies for targeting different types of protein surfaces using mimetics of protein secondary or tertiary structure. In this way strong and selective binding to a protein surface has be achieved and disruption of clinically important protein-protein interactions has been demonstrated in models of human disease.
Assuntos
Bioquímica/métodos , Proteínas/química , Proteínas/metabolismo , Sequência de Aminoácidos , Quimotripsina/antagonistas & inibidores , Quimotripsina/efeitos dos fármacos , Quimotripsina/metabolismo , Citocromo-c Peroxidase/química , Citocromo-c Peroxidase/efeitos dos fármacos , Citocromo-c Peroxidase/metabolismo , Citocromos c/química , Citocromos c/efeitos dos fármacos , Citocromos c/metabolismo , Mimetismo Molecular , Dados de Sequência Molecular , Compostos Orgânicos/química , Compostos Orgânicos/metabolismo , Compostos Orgânicos/farmacologia , Fator de Crescimento Derivado de Plaquetas/química , Fator de Crescimento Derivado de Plaquetas/metabolismo , Conformação Proteica , Proteínas/efeitos dos fármacos , Receptores do Fator de Crescimento Derivado de Plaquetas/química , Receptores do Fator de Crescimento Derivado de Plaquetas/efeitos dos fármacos , Receptores do Fator de Crescimento Derivado de Plaquetas/metabolismoRESUMO
Molecular oxygen's unique involvement in electron-transfer processes is demonstrated on a series of dyads between porphyrin derivatives and fullerene C60. It has been shown for the first time that oxygen can serve as an inhibitor of back electron transfer by enhancing intersystem crossing of a singlet radical ion pair into its triplet state. The effect is observed only when energy of the charge-separated state is lower than that of the locally excited triplet states. Due to the spin statistics, the reverse intersystem crossing is less efficient, allowing use of oxygen and other paramagnetic species for impeding charge recombination in various electron-transfer systems.
Assuntos
Carbono/química , Fulerenos , Metaloporfirinas/química , Oxigênio/química , Porfirinas/química , Elétrons , Mimetismo Molecular , Fotoquímica , FotossínteseRESUMO
The rational design of low-molecular weight ligands that disrupt protein-protein interactions is still a challenging goal in medicinal chemistry. Our approach to this problem involves the design of molecular scaffolds that mimic the surface functionality projected along one face of an alpha-helix. Using a terphenyl scaffold, which in a staggered conformation closely reproduces the projection of functionality on the surface of an alpha-helix, we designed mimics of the pro-apoptotic alpha-helical Bak-peptide as inhibitors of the Bak/Bcl-xL interaction. This led to the development of a potent Bcl-xL antagonist (KD = 114 nM), whose binding affinity for Bcl-xL was assessed by a fluorescence polarization assay. To determine the binding site of the developed inhibitor we used docking studies and an HSQC-NMR experiment with 15N-labeled Bcl-xL protein. These studies suggest that the inhibitor is binding in the same hydrophobic cleft as the Bak- and Bad-peptides.