RESUMO
We have identified a homologue of the GTP-binding protein, Sar1p, in Plasmodium falciparum. Sar1p is a small GTPase that is thought to play a crucial role in trafficking of proteins between the endoplasmic reticulum and the Golgi. The P.falciparum SAR1 gene is located on chromosome 4 and comprises two exons separated by a 508 bp intron. The deduced amino acid sequence of PfSar1p (GenBank accession number AF104306) shows 71% similarity (58% identity) to Sar1p from Saccharomyces cerevisiae. Expression of PfSar1p in erythrocytic stages of P. falciparum was confirmed by sequencing of a tryptic peptide derived from a polypeptide excised from an SDS-polyacrylamide gel. A recombinant protein corresponding to approximately 70% of the PfSar1p sequence was used to raise antibodies. The affinity-purified antiserum recognised a protein with an apparent molecular weight of 23 K in Western blots of malaria-infected erythrocytes but not in uninfected erythrocytes. PfSar1p was shown to be largely insoluble in non-ionic detergent and a low ionic strength buffer. Confocal immunofluorescence microscopy of malaria-infected erythrocytes was used to show that PfSar1p is located near the periphery of the parasite in discrete compartments, which appear to be distinct from the parasite endoplasmic reticulum. In addition, PfSar1p appears to be exported to structures outside the parasite in the erythrocyte cytoplasm. The export of PfSar1p to the erythrocyte cytosol is inhibited by treatment with brefeldin A. This provides the first evidence that the malaria parasite is capable of elaborating components of the classical vesicle-mediated trafficking machinery outside the boundaries of its own plasma membrane.
Assuntos
Eritrócitos/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Malária Falciparum/sangue , Proteínas Monoméricas de Ligação ao GTP , Plasmodium falciparum/metabolismo , Proteínas de Protozoários/metabolismo , Proteínas de Saccharomyces cerevisiae , Sequência de Aminoácidos , Animais , Transporte Biológico/efeitos dos fármacos , Brefeldina A/farmacologia , Citosol/metabolismo , Eritrócitos/parasitologia , Proteínas de Ligação ao GTP/genética , Soros Imunes , Microscopia Confocal , Microscopia de Fluorescência , Dados de Sequência Molecular , Plasmodium falciparum/genética , Proteínas de Protozoários/genética , Proteínas Recombinantes de Fusão/imunologia , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Solubilidade , Especificidade da Espécie , Proteínas de Transporte VesicularRESUMO
An endoplasmic reticulum-located, calcium-binding protein, with an apparent molecular weight (Mr) of approximately 40,000 (PfERC), has been identified in the asexual stages of the malaria parasite, Plasmodium falciparum. This protein appears to be equivalent to a previously described gametocyte protein, Pfs40, which was reported to be expressed on the gametocyte surface (Rawlings DJ, Kaslow DC. J Biol Chem 1992;267:3976-3982). Sequencing of the 3' end of the gene revealed the omission of a single base in the 3' region of the published sequence. The corrected gene sequence encodes a C-terminal IDEL motif, which indicates residency of the 40 kDa protein within the endoplasmic reticulum. The predicted C-terminal region also appears to contain a sixth EF-hand calcium-binding domain, which suggests that PfERC is related to previously reported ER-localized calcium-binding proteins, namely reticulocalbin and ERC-55 (Ozawa M. J. Biochem. 1995;117:1113-1119; Weis K, Griffiths G, Lamond AI. J. Biol. Chem. 1994;269:19142-19150). The presence of the 40 kDa calcium-binding protein in malaria parasites was confirmed using 45Ca2+-blotting and partial protein sequencing of the corresponding Coomassie blue-stained polypeptide. Confocal immunofluorescence microscopy of asexual stage parasites was used to show that PfERC co-localizes with the known ER-located protein, Pfgrp. Analysis of immunoblots of tightly synchronized parasites showed that expression of PfERC increases with increasing maturity of the parasite. We propose that PfERC is a member of the reticulocalbin family of calcium-binding proteins and may play a role in protein trafficking in the malaria parasite.
Assuntos
Proteínas de Ligação ao Cálcio/genética , Retículo Endoplasmático/química , Plasmodium falciparum/genética , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Animais , Proteínas de Ligação ao Cálcio/análise , Proteínas de Ligação ao Cálcio/química , Eritrócitos/parasitologia , Regulação da Expressão Gênica no Desenvolvimento , Genes de Protozoários/genética , Humanos , Dados de Sequência Molecular , Peso Molecular , Plasmodium falciparum/crescimento & desenvolvimento , Proteínas de Protozoários/análise , Proteínas de Protozoários/química , Análise de Sequência , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
A photoreactive quinolinemethanol analog, N-[4-[1-hydroxy-2-(dibutylamino)ethyl]quinolin-8yl]-4- azido-2-salicylamide (ASA-MQ) has been synthesized which closely mimics the action of mefloquine. ASA-MQ possesses potent antimalarial activity against a mefloquine-sensitive strain of Plasmodium falciparum and shows decreased activity against a mefloquine-resistant parasite strain. Radioiodinated ASA-MQ has been used in photoaffinity labeling studies to identify mefloquine-interacting proteins in serum, uninfected erythrocytes and Plasmodium falciparum-infected erythrocytes. We have shown that mefloquine interacts specifically with apo-A1, the major protein of serum high density lipoproteins. In addition, mefloquine was shown to interact specifically with the erythrocyte membrane protein, band 7.2b (stomatin). A further two high affinity mefloquine-binding proteins with apparent molecular masses of 22 and 36 kDa were identified in three different strains of Plasmodium falciparum. We suggest that these two mefloquine-binding parasite proteins may be involved in the uptake of mefloquine or may represent macromolecular targets of mefloquine action in malaria parasites.
Assuntos
Antimaláricos/metabolismo , Proteínas Sanguíneas/metabolismo , Mefloquina/metabolismo , Proteínas de Membrana , Plasmodium falciparum/metabolismo , Proteínas de Protozoários/metabolismo , Marcadores de Afinidade , Animais , Apolipoproteína A-I/metabolismo , Resistência a Medicamentos , Membrana Eritrocítica/metabolismo , Humanos , Mefloquina/análogos & derivados , Mefloquina/farmacologiaRESUMO
A head-group modified, photoreactive analog of phosphatidylethanolamine, N-([125I]iodo-4-azidosalicy- lamidyl)-1,2-dilauryl-sn-glycero-3-phosphatidylethanolamine ([125I]-N-ASA-DLPE), has been used in photoaffinity labeling studies of proteins of the human erythrocyte membrane. [125I]-N-ASA-DLPE was shown to be preferentially incorporated into a protein with an apparent molecular weight of 31 kDa. Protein sequencing and immunoprecipitation were used to identify this protein as the erythrocyte membrane protein, band 7.2b or stomatin. A sulphydryl-reactive ligand, 4-hydroxy-3-(iodo-[125I])-N-[2-(2-pyridinyldithio)ethyl]- benzenepropanamide ([125I]-PDA), was also shown to preferentially label band 7.2b. We propose that band 7.2b may act as a site of transbilayer reorientation of membrane phospholipids.
Assuntos
Azidas/metabolismo , Proteínas Sanguíneas/metabolismo , Membrana Eritrocítica/metabolismo , Proteínas de Membrana , Fosfatidiletanolaminas/metabolismo , Marcadores de Afinidade/síntese química , Marcadores de Afinidade/metabolismo , Azidas/síntese química , Proteínas Sanguíneas/química , Proteínas Sanguíneas/isolamento & purificação , Western Blotting , Eletroforese em Gel de Poliacrilamida , Humanos , Radioisótopos do Iodo , Modelos Estruturais , Fenilpropionatos/metabolismo , Fosfatidiletanolaminas/síntese química , Estrutura Secundária de Proteína , Piridinas/metabolismoRESUMO
Altered extracellular matrix production by the glomerular mesangium is a feature of diabetes mellitus. Matrix proteins, including fibronectin, via interaction with cell-surface receptors (the integrins) may activate intracellular pathways such as prostaglandin production, shown previously to be stimulated by addition of fibronectin to glomerular cores. However, the signalling pathways involved are unclear. An intracellular tyrosine kinase (focal adhesion kinase), associated with focal adhesions, is known to be phosphorylated after interaction with matrix proteins. We now show for the first time, in glomeruli from diabetic rats, that focal adhesion kinase has increased phosphorylation on tyrosine, when compared with non-diabetic control rats. This phosphorylation was labile and disappeared with extended time of sample preparation or digestion of glomeruli to glomerular cores. Cultured mesangial cells, from non-diabetic rats, plated onto fibronectin also showed increased tyrosine phosphorylation of focal adhesion kinase accompanied by a twofold increase in prostaglandin production. However, it may not be possible to replicate fully the diabetic ¿state¿ in vitro merely by use of raised glucose concentrations, as these conditions (for 3 weeks) resulted in decreased focal adhesion kinase phosphorylation, despite increased fibronectin and prostaglandin levels. A role for increased focal adhesion kinase phosphorylation in kidney glomeruli isolated from diabetic rats, and any linkage to intracellular signalling pathways remains to be determined.