Reprogramming of 3D genome structure underlying HSPC development in zebrafish.

BACKGROUND: Development of hematopoietic stem and progenitor cells (HSPC) is a multi-staged complex process that conserved between zebrafish and mammals. Understanding the mechanism underlying HSPC development is a holy grail of hematopoietic biology, which is helpful for HSPC clinical application. Chromatin conformation plays important roles in transcriptional regulation and cell fate decision; however, its dynamic and role in HSPC development is poorly investigated. METHODS: We performed chromatin structure and multi-omics dissection across different stages of HSPC developmental trajectory in zebrafish for the first time, including Hi-C, RNA-seq, ATAC-seq, H3K4me3 and H3K27ac ChIP-seq. RESULTS: The chromatin organization of zebrafish HSPC resemble mammalian cells with similar hierarchical structure. We revealed the multi-scale reorganization of chromatin structure and its influence on transcriptional regulation and transition of cell fate during HSPC development. Nascent HSPC is featured by loose conformation with obscure structure at all layers. Notably, PU.1 was identified as a potential factor mediating formation of promoter-involved loops and regulating gene expression of HSPC. CONCLUSIONS: Our results provided a global view of chromatin structure dynamics associated with development of zebrafish HSPC and discovered key transcription factors involved in HSPC chromatin interactions, which will provide new insights into the epigenetic regulatory mechanisms underlying vertebrate HSPC fate decision.

Layered Double Hydroxide Nanosheets Boosting Red Long Afterglow via Highly Efficient Energy Transfer.

Room-temperature phosphorescent (RTP) based long-afterglow materials have shown broad application prospects in smart sensors, biological imaging, photodynamic therapy, and many others. However, the fabrication of red long-afterglow materials still faces a great challenge due to the competitive relationship between RTP efficiency and lifetime. In this work, we reported a series of layered double hydroxide (LDHs) nanosheets with red long-afterglow (quantum yield up to 42.35% and lifetime up to 256.77 ms) by taking advantage of the highly efficient triplet-triplet energy transfer from green phosphorescent LDHs to the red fluorescent dye rhodamine B (RhB, as a guest molecule). Specifically, the Zn-based LDHs@RhB composite (Zn-Al-LDH-4-CBBA@RhB) presents energy transfer efficiency as high as 95.18%, and the red long-afterglow could even be excited upon white-light irradiation. Benefiting from the time-resolved afterglow, the LDHs@RhB composites exhibit great potential in the fields of anticounterfeiting and information encryption.

Risk factors of serious postoperative outcomes in patients aged ≥90 years undergoing surgical intervention.

Objective: We aimed to identify preoperative and intraoperative factors associated with serious postoperative outcomes, which may help patients and clinicians make better-informed decisions. Methods: We conducted a retrospective study including all patients aged ≥90 years who underwent surgery between January 1, 2011, and January 1, 2021, at Chongqing University Central Hospital. We assessed 30 pre- and intraoperative demographic and clinical variables. Logistic regression was used to identify the independent risk factors for serious postoperative outcomes in patients aged ≥90 years. Results: A total of 428 patients were included in our analysis. The mean age was 92.6 years (SD ± 2.6). There were 240 (56.1%) females and 188 (43.9%) males. The most common comorbidities were hypertension (44.9%) and arrhythmias (34.8%). The 30-day hospital mortality was 5.6%, and severe morbidity was 33.2%. Based on the multivariate logistic regression classification analysis of the American Society of Anesthesiologists (ASA)≥ Ⅳ [odds ratio (OR), 5.39, 95% confidence interval (CI), 2.06-14.16, P = .001], emergency surgery (OR, 5.02, 95% CI, 2.85-15.98, P = .001) and chronic heart failure (OR, 6.11, 95% CI, 1.93-13.06, P = .001) were identified as independent risk factors for 30-day hospital mortality, and ASA≥ Ⅳ (OR, 4.56, 95%CI, 2.56-8.15, P < .001), Barthel index (BI) < 35 (OR, 2.28, 95%CI, 1.30-3.98, P = .001), chronic heart failure (OR, 3.67, 95%CI, 1.62-8.31, P = .002), chronic kidney disease (OR, 4.24, 95%CI, 1.99-9.05, P < .001), general anesthesia (OR, 3.31, 95%CI, 1.91-5.76, P < .001), emergency surgery (OR, 3.72, 95%CI, 1.98-6.99, P < .001), and major surgery (OR, 3.44, 95%CI, 1.90-6.22, P < .001) were identified as independent risk factors for serious postoperative complications. Conclusions: Patients aged ≥90 years with ASA≥ Ⅳ, BI < 35, combined with chronic heart failure or chronic kidney disease, undergoing emergency surgery, major surgery or general anesthesia have a higher risk of serious postoperative outcomes. Identifying these risk factors in an early stage may contribute to our clinical decision-making and improve the quality of treatments.

Reverse Microemulsion Synthesis of Fe3O4-Ag2S Heteronanocrystals for Dual-Modal Imaging-Guided Photothermal Tumor Ablation.

Heterojunction nanomaterials have revealed a significant possibility in tumor diagnosis and therapeutic owing to tightly combining two parts of different chemical properties. Herein, we successfully synthesized heterogenous Fe3O4-Ag2S nanocrystals through a reverse microemulsion. Based on the prepared heterostructure, a new drug nanoplatform was developed, which served as a contrast agent for T2-weighted magnetic resonance imaging, computed tomography imaging, and photothermal imaging. In addition, these Fe3O4-Ag2S heteronanocrystals exhibited a high photothermal conversion efficiency of 75.5% irradiated by a laser of 808 nm wavelength. After injection into glioma cancer mode in vivo, efficient tumor accumulation of Fe3O4-Ag2S heteronanocrystals was observed under triple-modal imaging. The prepared heteronanocrystals further showed an excellent ablated tumor destruction effect through the generated hyperthermia with the irradiation of an 808 nm laser. Our work suggests that the potential of Fe3O4-Ag2S heteronanocrystals may be a promising theranostic agent for multimodal imaging guidance and photothermal therapy toward cancer.