Femtosecond laser optimization of PEEK: efficient bioactivity achieved by synergistic surface chemistry and structures.

Poly-ether-ether-ketone (PEEK) is considered a potential orthopedic material due to the excellent mechanical properties and chemical resistance, but its biological inertness hampers its further clinical application. In this study, advanced femtosecond laser microfabrication technology was utilized to induce the change of the surface characteristics of PEEK to improve its bioactivity. Meanwhile, the mechanism of surface reaction and improved bioactivity was interpreted in detail from the perspective of material science. The surface physical-chemical characterization results showed that femtosecond laser etching could increase the surface energy, and the contents of active sites including amorphous carbon and carbon-hydroxyl on PEEK surfaces. In vitro validation experiments demonstrated that the samples etched with a femtosecond laser had a better ability to induce apatite deposition and cell proliferation than those treated with popular sulfonation modification, which would lead to better bioactivity and osteointegration. The current work fully presents the mechanism of the femtosecond laser low-temperature plasma effect on PEEK and the resulting surface characteristics, which could broaden the application of PEEK in the orthopedic field. Moreover, it has great potential in the surface design and modification of other biomaterials with enhanced bioactivity.

Host defense against the infection of Klebsiella pneumoniae: New strategy to kill the bacterium in the era of antibiotics?

Klebsiella pneumoniae (K. pneumoniae) is a typical gram-negative iatrogenic bacterium that often causes bacteremia, pneumonia and urinary tract infection particularly among those with low immunity. Although antibiotics is the cornerstone of anti-infections, the clinical efficacy of ß-lactamase and carbapenems drugs has been weakened due to the emergence of drug-resistant K. pneumoniae. Recent studies have demonstrated that host defense plays a critical role in killing K. pneumoniae. Here, we summarize our current understanding of host immunity mechanisms against K. pneumoniae, including mechanical barrier, innate immune cells, cellular immunity and humoral immunity, providing a theoretical basis and the new strategy for the clinical treatment of K. pneumoniae through improving host immunity.