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Herein, we show a composite formation method of tin/tin oxide nanoparticles with graphene oxide and CMC based on laser ablation technique as an electrode material for energy storage devices. The material exhibited a three-dimensional conducting graphene oxide network decorated with tin or tin oxide nanoparticles. The structure, homogeneous distribution of nanoparticles, and direct contact between inorganic and organic parts were confirmed by scanning electron microscopy and high-resolution transmission electron spectroscopy. Electrochemical performances of composite electrode material showed a reversible capacity of 644 mAh/g at a current density equal to 35 mA/g, and 424 mAh/g at 140 mA/g. The capacity retention of 90% after 250 cycles show that tested electrode material is suitable as a negative electrode for lithium-ion batteries.
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Millions of dollars have been spent on the design and dissemination of educational materials to improve handwashing to prevent infectious diseases. School-age children have been the focus of many of these efforts; yet little is known about the content of these materials. This study uses content analysis to examine the theoretical and motivational trends as well as the communication approach used in a sample of hand hygiene intervention materials targeting elementary-age children. Two trained coders analyzed 144 communication materials. Study results indicate that educational materials infrequently exhibit information consistent with theories of communication for behavior change, commonly use fear-based messaging, and rarely recommend using technology in the design of the interventions. Implications for future research and the design of more strategic, child-focused hand hygiene interventions are discussed.
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Higiene das Mãos/métodos , Educação em Saúde/métodos , Educação em Saúde/estatística & dados numéricos , Criança , Pré-Escolar , Comunicação , Medo , Feminino , Desinfecção das Mãos/métodos , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Motivação , Estados UnidosRESUMO
This paper explores the connection between the H3BO3 flux concentration and the co-existence of Eu2+ and Eu3+ dopants within CaMgSi2O6 crystals (diopside). The samples were synthesised using a solid-state synthesis method under varying atmospheric conditions, including oxidative (air), neutral (N2), and reductive (H2/N2 mixture) environments. Additionally, some materials underwent chemical modification by partially substituting Si4+ with Al3+ ions acting as charge compensation defects stabilizing Eu3+ luminescence. Depending on the specific synthesis conditions, the materials predominantly displayed either the orange-red luminescence of Eu3+ (under oxidising conditions) or the blue luminescence of Eu2+; however, the comprehensive results confirmed the co-existence of Eu3+/Eu2+ luminescence in both cases. This work shows that varying flux concentrations added during synthesis significantly affect the relative strength of Eu2+ and Eu3+ emissions in a manner dependent on the synthesis atmosphere. The emission of Eu2+ increases with a higher flux concentration in materials synthesised under oxidative and neutral atmospheres independent of the chemical modification. In contrast, for materials obtained under a reductive atmosphere, the changes in the Eu3+ emission intensity depended on the presence or absence of Al3+ ions namely the increase of flux increased the Eu3+ intensity in the case of unmodified materials and decreased in the Al-modified ones. All observed effects were qualitatively explained considering the double role of the flux in the studied system, which besides facilitating the diffusion of chemical species during synthesis acts as a charge compensating agent by creating B'Si centres stabilizing Eu3+ emission.
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A series of strontium orthotitanate (Sr2TiO4) samples doped with 2% of a mole of europium, praseodymium, and erbium were obtained using the solid-state synthesis method. The X-ray diffraction (XRD) technique confirms the phase purity of all samples and the lack of the influence of dopants at a given concentration on the structure of materials. The optical properties indicate, in the case of Sr2TiO4:Eu3+, two independent emission (PL) and excitation (PLE) spectra attributed to the Eu3+ ions at sites with different symmetries: low - excited at 360 nm and high - excited at 325 nm, while, for Sr2TiO4:Er3+ and Sr2TiO4:Pr3+, the emission spectra do not depend on the excitation wavelength. The measurements of X-ray photoemission spectroscopy (XPS) indicate the presence of only one type of charge compensation mechanism, which is based on the creation of strontium vacancies in all cases. This suggests that the different charge compensation mechanisms cannot easily explain the presence of Eu3+ at two non-equivalent crystal sites. The photocurrent excitation (PCE) spectroscopy investigations, that have not been reported in the literature so far, show that among all the studied dopants, only Pr3+ can promote the electrons to the conduction band and give rise to electron conductivity. The results collected from the PLE and PCE spectra allowed us to find the location of the ground states of lanthanides(II)/(III) in the studied matrix.
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In this work, ZnIn2S4 layers were obtained on fluorine doped tin oxide (FTO) glass and TiO2 nanotubes (TiO2NT) using a hydrothermal process as photoanodes for photoelectrochemical (PEC) water splitting. Then, samples were annealed and the effect of the annealing temperature was investigated. Optimization of the deposition process and annealing of ZnIn2S4 layers made it possible to obtain an FTO-based material generating a photocurrent of 1.2 mA cm-2 at 1.62 V vs. RHE in a neutral medium. In contrast, the highest photocurrent in the neutral electrolyte obtained for the TiO2NT-based photoanode reached 0.5 mA cm-2 at 1.62 V vs. RHE. In addition, the use of a strongly acidic electrolyte allowed the generated photocurrent by the TiO2NT-based photoanode to increase to 3.02 mA cm-2 at 0.31 V vs. RHE. Despite a weaker photoresponse in neutral electrolyte than the optimized FTO-based photoanode, the use of TiO2NT as a substrate allowed for a significant increase in the photoanode's operating time. After 2 h of illumination, the photocurrent response of the TiO2NT-based photoanode was 0.21 mA cm-2, which was 42% of the initial value. In contrast, the FTO-based photoanode after the same time generated a photocurrent of 0.02 mA cm-2 which was only 1% of the initial value. The results indicated that the use of TiO2 nanotubes as a substrate for ZnIn2S4 deposition increases the photoanode's long-term stability in photoelectrochemical water splitting. The proposed charge transfer mechanism suggested that the heterojunction between ZnIn2S4 and TiO2 played an important role in improving the stability of the material by supporting charge separation.
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Water-soluble upconversion nanoparticles (UCNPs), based on polyvinylpyrrolidone (PVP)-coated NaYF4:Er3+,Yb3+,Gd3+, with various concentrations of Gd3+ ions and relatively high upconversion efficiencies, were synthesized. The internalization and cytotoxicity of the thus obtained UCNPs were evaluated in three cell lines (HeLa, HEK293 and astrocytes). No cytotoxicity was observed even at concentrations of UCNPs up to 50 µg ml-1. The fate of the UCNPs within the cells was studied by examining their upconversion emission spectra with confocal microscopy and confirming these observations with transmission electron microscopy. It was found that the cellular uptake of the UCNPs occurred primarily by clathrin-mediated endocytosis, whereas they were secreted from the cells via lysosomal exocytosis. The results of this study, focused on the mechanisms of the cellular uptake, localization and secretion of UCNPs, demonstrate, for the first time, the co-localization of UCNPs within discrete cell organelles.
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BACKGROUND: Autosomal dominant cancer syndrome--multiple endocrine neoplasia type 2 (MEN 2), may exist more often than expected in patients with pheochromocytoma. Germline mutations identified recently in MEN 2 can be revealed by genetic screening. OBJECTIVE: To evaluate the frequency of RET (rearranged during transfection) mutations in patients with pheochromocytoma. DESIGN AND METHODS: We genetically screened germline mutations in the RET proto-oncogene and clinically re-evaluated patients with pheochromocytoma. A pentagastrin test and other biochemical studies were performed in all patients. SETTING: Department of Internal Medicine and Hypertension, The Medical University of Warsaw, Warsaw, Poland and the Department of Nephrology and Hypertension, Albert Ludwigs University, Freiburg, Germany. PARTICIPANTS: Seventy seven unselected patients with pheochromocytoma (19 men, 58 women, mean age: 51.55 +/- 1.5 years; pheochromocytoma confirmed histopathologically) out of 162 diagnosed and treated in the years 1957-1998 in the Department of Internal Medicine and Hypertension in Warsaw, Poland. The other 85 patients did not respond to the written invitation. MAIN OUTCOME MEASURES: The finding of RET mutations and diagnosis of MEN 2 in patients with pheochromocytoma. RESULTS: Genetic testing revealed germline mutations in the RET proto-oncogene in six patients (7.8%). All carriers had mutation of exon 11, codon 634: TGC to CGC. In four patients with this mutation, medullary thyroid carcinoma (MIC) was diagnosed and in three cases, surgically treated. Biochemical parameters: parathormone 31.88 +/- 2.87 pg/ml, calcitonin: 0 min 0.23 +/- 0.14 ng/ml; 2 min 0.49 +/- 0.21 ng/ml; 5 min 0.48 +/- 0.21 ng/ml, metoxycatecholamines: 601.62 +/- 42.71 microg/24h, epinephrine: 1.94 +/- 0.17 microg/24h, norepinephrine 13.96 +/- 1.3 microg/24h, carcinoembryonic antigen (CEA) 9.94 +/- 4.3 ng/ml. Ambulatory blood pressure monitoring (ABPM): systolic blood pressure (SBP): 116 +/- 1.9 mmHg, diastolic blood pressure (DBP): 73.7 +/- 0.9 mmHg. Clinical, biochemical and imaging procedures did not reveal any recurrence of pheochromocytoma in the 77 patients studied. CONCLUSIONS: Patients with pheochromocytoma should be genetically screened for mutations of the RET proto-oncogene. These patients should undergo clinical screening for MEN 2. In addition, genetic studies can be useful for the screening of the families of the carriers.
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Neoplasias das Glândulas Suprarrenais/genética , Proteínas de Drosophila , Mutação em Linhagem Germinativa/genética , Feocromocitoma/genética , Proteínas Proto-Oncogênicas/genética , Receptores Proteína Tirosina Quinases/genética , Neoplasias das Glândulas Suprarrenais/epidemiologia , Adulto , Monitorização Ambulatorial da Pressão Arterial , Feminino , Frequência do Gene , Humanos , Masculino , Hormônio Paratireóideo/sangue , Linhagem , Pentagastrina , Feocromocitoma/epidemiologia , Polônia/epidemiologia , Proto-Oncogene Mas , Proteínas Proto-Oncogênicas c-retRESUMO
The aim of the study was to investigate endothelin-1 (ET-1) and neuropeptide Y (NPY) plasma concentrations in renal venous blood of hypertensive patients with unilateral renal artery stenosis (URAS). The study was performed in 22 patients with URAS and 18 patients diagnosed as essentially hypertensive. In each subject renal arteriography and renal vein catheterisation was performed. Blood samples for ET-1, NPY and plasma renin activity (PRA) were withdrawn from renal veins and vena cava inferior, and for ET-1 and NPY from the aorta. Patients with URAS were divided in two subgroups according to the renal vein renin ratio. Both in nine patients with URAS and ratio > 1.5 and in 13 patients with URAS and ratio < 1.5, ET-1 and NPY plasma concentrations evaluated in renal venous blood of the ischaemic kidney were not different from those assessed in the contralateral side, in vena cava inferior and in the aorta. In essential hypertension, the mean ET-1 and NPY plasma concentrations of both renal veins were not different from the ET-1 and NPY plasma values assessed in renal vein of stenosed and contralateral side, vena cava and aorta of patients with URAS with and without activation of the renin system. Our study indicates that chronic ischaemia does not affect ET-1 and NPY plasma concentrations in renal venous blood of hypertensive URAS patients both with and without activation of the renin system.
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Endotelinas/sangue , Hipertensão/sangue , Neuropeptídeo Y/sangue , Obstrução da Artéria Renal/sangue , Adulto , Feminino , Humanos , Hipertensão/complicações , Masculino , Pessoa de Meia-Idade , Obstrução da Artéria Renal/complicações , Obstrução da Artéria Renal/diagnóstico , Veias RenaisRESUMO
The aim of the study was to investigate blood pressure responses and changes in heart rate after bolus administration of endothelin-3 [ET-3] in conscious, freely moving SHR and WKY rats and DOCA-salt hypertensive and normotensive Wistar rats. The effect of ET-3 on blood pressure and heart rats was investigated for four doses equal to 250 ng, 500 ng, 1000 ng and 2000 ng of ET-3. Our study shows that in experimental models of hypertension changes in blood pressure were predominantly characterized by the pronounced hypotensive phase with no significant raises in blood pressure. In normotensive animals cardiovascular responses to ET-3 were biphasic, with initial depressor phase followed by long-lasting, significant pressor effect.
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Pressão Sanguínea/efeitos dos fármacos , Endotelinas/farmacologia , Hipertensão/fisiopatologia , Animais , Peso Corporal/efeitos dos fármacos , Desoxicorticosterona , Frequência Cardíaca/efeitos dos fármacos , Hipertensão/induzido quimicamente , Masculino , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Ratos WistarRESUMO
The aim of the study was to find out whether vasopressin (AVP) modifies hypotensive and heart rate accelerating effects of atrial natriuretic peptide (ANP) in normotensive (WKY) and spontaneously hypertensive (SHR) conscious rats. The effect of i.v. administration of 1; 2 and 4 micrograms of ANP on blood pressure (MP) and heart rate (HR) was compared during i.v. infusion of 0.9% NaCl (NaCl), NaCl+AVP (1.2 ng kg-1 min-1) and NaCl+dEt2AVP (V1 receptors antagonist, 0.5 microgram kg-1 min-1). AVP increased MP in SHR and WKY and decreased HR in SHR. V1 antagonist decreased MP and increased HR only in SHR. In SHR ANP decreased MP and increased HR during NaCl, AVP and V1 antagonist infusion. In WKY these effects were observed only during AVP administration. In each experimental situation hypotension and tachycardia induced by ANP were greater in SHR than in WKY. In both strains ANP induced changes in MP and HR were enhanced during AVP in comparison to NaCl infusion. V1 antagonist did not modify effects of ANP in WKY and SHR. The results indicate that ANP abolishes hypertensive response induced by blood AVP elevation and that the basal levels of endogenous vasopressin acting through V1 receptors does not interfere with hypotensive action of ANP neither in WKY nor in SHR.
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Antagonistas dos Receptores de Hormônios Antidiuréticos , Arginina Vasopressina/farmacologia , Fator Natriurético Atrial/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Animais , Arginina Vasopressina/sangue , Fator Natriurético Atrial/fisiologia , Pressão Sanguínea/fisiologia , Interações Medicamentosas , Frequência Cardíaca/efeitos dos fármacos , Frequência Cardíaca/fisiologia , Hipotensão/induzido quimicamente , Masculino , Fragmentos de Peptídeos/farmacologia , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Cloreto de Sódio/farmacologiaRESUMO
Radon gas is a significant health threat linked to thousands of preventable deaths each year. One population that may be at increased risk from harm from radon exposure is African Americans. However, little is known about what African Americans think or know about radon. A theoretically based evaluation of radon awareness and reduction campaigns was conducted with African Americans. The knowledge and perceptions results indicate that African Americans often hold inaccurate beliefs regarding radon (e.g., confusing it with carbon monoxide gas), perceive it to be a serious threat, and perceive recommended responses to be inadequate in averting harm. The campaign materials evaluation shows that campaign materials often promote perceptions of threat but not perceptions of efficacy regarding recommended responses. Recommendations are given for public health practitioners.
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Negro ou Afro-Americano , Exposição Ambiental/prevenção & controle , Educação em Saúde/métodos , Conhecimentos, Atitudes e Prática em Saúde , Radônio , Adulto , Idoso , Grupos Focais , Humanos , Michigan , Pessoa de Meia-Idade , Radônio/efeitos adversos , Materiais de EnsinoRESUMO
Serotonin appears to play an important part in the pathogenesis of essential hypertension. Various studies have shown, that the metabolism of serotonin may be disturbed in some pathological conditions for example in hypertension. It concerns also the changed mechanisms of uptake and release of serotonin. The certain blood vessels may become more hypersensitive to the vasoconstrictor effects of serotonin in patients with hypertension than in normal subjects. During chronic treatment with ketanserin, S2-serotonergic antagonist, blood pressure is reduced in spontaneously hypertensive rats and in humans. This fact can also indicate indirectly, that that serotonin plays a part in the pathogenesis of essential hypertension. The aim of the study was to determine the concentration of free serotonin (S) in the blood of 15 patients with sustained essential hypertension in the mean age 32.8 +/- 1.8, of 23 patients with borderline essential hypertension in the mean age 29.0 +/- 3.0 and of 10 normal subjects in the mean age 31.1 +/- 1.7 years. Plasma free serotonin was determined by fluorometric method. All patients and controls were investigated at the hospital. They were on normosodium diet, without drugs for last two weeks. The fasting blood samples were collected in the supine position. Free serotonin blood concentration was significantly higher in hypertensive group than in normal subjects. The important difference of serotonin blood concentration between two groups of hypertensive patients was noticed. It was significantly higher in group of patients with sustained hypertension, than in group with borderline hypertension (p less than 0.05). Our results are similar to the observations of other authors.(ABSTRACT TRUNCATED AT 250 WORDS)
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Hipertensão/sangue , Serotonina/sangue , Adulto , Humanos , Hipertensão/etiologia , Masculino , Valores de ReferênciaRESUMO
We evaluated the effect of captopril treatment on left ventricular mass (LVM) and function in sustained mild-to-moderate primary hypertension. In 16 men aged 34.5 +/- 6.4 yrs. M-mode echo and pulsed wave Doppler recordings were obtained prior to and after 2 months captopril treatment (50-150 mg daily). No significant changes in heart rate, systolic blood pressure, ejection fraction, fractional shortening, cardiac output, LVM and Doppler-derived indices of mitral inflow were observed. Diastolic blood pressure measured during examination fell from 102.2 +/- 13.3 to 90.3 +/- 9 mmHg (p less than 0.026). No consistent correlations between catecholamines and indexes of left ventricular function were found. Two-month captopril therapy in mild-to-moderate hypertension results in diastolic blood pressure lowering with no effect on LVM or left ventricular function.
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Captopril/uso terapêutico , Catecolaminas/sangue , Hipertensão/tratamento farmacológico , Renina/metabolismo , Função Ventricular Esquerda/efeitos dos fármacos , Adulto , Ecocardiografia Doppler , Humanos , Hipertensão/sangue , Hipertensão/diagnóstico por imagem , Hipertensão/fisiopatologia , MasculinoRESUMO
Atrial natriuretic peptide (ANP) activity was studied in 33 males with borderline and established essential hypertension. No significant differences of serum ANP concentration were stated in patients with borderline and established uncomplicated hypertension in comparison with the control group.
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Fator Natriurético Atrial/sangue , Hipertensão/sangue , Adolescente , Adulto , Humanos , Hipertensão/etiologia , Masculino , Pessoa de Meia-IdadeRESUMO
Atrial natriuretic peptide concentrations (ANP) were determined in 4 patients with pheochromocytoma during its surgical removal. There was no correlation among ANP level, plasma catecholamines concentrations and blood pressure. Increase of plasma ANP concentration after tumor removal can be explained by simultaneously observed hypervolemia. It proves that ANP secretion mostly depends on enlarged central blood volume.
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Neoplasias das Glândulas Suprarrenais/cirurgia , Fator Natriurético Atrial/sangue , Epinefrina/sangue , Norepinefrina/sangue , Feocromocitoma/cirurgia , Neoplasias das Glândulas Suprarrenais/fisiopatologia , Adulto , Pressão Sanguínea/fisiologia , Volume Sanguíneo/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Feocromocitoma/fisiopatologiaRESUMO
Objective of the study was assessment of the usefulness of determination of noradrenaline (NA) and adrenaline (A) in urine and blood as well as the total methoxycatecholamines (MNA +MA), vanillylmandelic acid (VMA), DOPA and dopamine (DA) urinary excretion in diagnosis of pheochromocytoma. The experience based on 155 patients with pheochromocytoma (105F, 50M, age 18-82 yrs) diagnosed in the Department of Hypertension and Angiology Academy of Medicine in Warsaw will be discussed. In all patients excluding 2 cases pheochromocytoma has been proven histopathologically. The most considerable diagnostic usefulness of MNA + MA indication was proven. MNA + MA was increased in 96.6 patients. In 89.6% patients an increased excretion of NA and A or one of this catecholamines was demonstrated. An increased excretion of VMA was demonstrated in 75%. The excretion of DOPA and dopamine was tested in 120 cases. An increased excretion of DA was shown in 31% and DOPA in 16%.
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Neoplasias das Glândulas Suprarrenais/diagnóstico , Catecolaminas/análise , Feocromocitoma/diagnóstico , Neoplasias das Glândulas Suprarrenais/sangue , Neoplasias das Glândulas Suprarrenais/urina , Adulto , Idoso , Idoso de 80 Anos ou mais , Di-Hidroxifenilalanina/urina , Dopamina/urina , Epinefrina/sangue , Epinefrina/urina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Norepinefrina/sangue , Norepinefrina/urina , Feocromocitoma/sangue , Feocromocitoma/urina , Ácido Vanilmandélico/urinaRESUMO
The arterial pressure, the pulse frequency, the concentration of arterial natriuretic peptide (ANP), the aldosterone++ concentration and the plasma renin activity were determined in patients with primary hypertension after administration of furosemide in conditions of active assuming of erect position. The ANP concentration was lowered in that conditions both in the patients and in healthy subjects.
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Fator Natriurético Atrial/sangue , Furosemida/farmacologia , Hipertensão/fisiopatologia , Postura/fisiologia , Adulto , Fator Natriurético Atrial/antagonistas & inibidores , Depressão Química , Humanos , Masculino , Pessoa de Meia-Idade , Sistema Renina-Angiotensina/fisiologia , Fatores de TempoRESUMO
Plasma atrial natriuretic factor (ANF) and catecholamine concentrations were determined in 11 untreated patients with uncomplicated essential hypertension and seven normotensive subjects during graded submaximal exercise on a treadmill (loads: I 40%, II 60%, III 80% of maximal oxygen consumption). No significant differences in plasma ANF and catecholamine concentrations were found between the hypertensive and the normotensive subjects. The results do not support the presence of significant differences in the release of ANF and catecholamines during dynamic exercise between patients with uncomplicated essential hypertension and normotensive subjects.