Metaphase Cells Enrichment for Efficient Use in the Dicentric Chromosome Assay.

The dicentric chromosome assay (DCA), is considered the 'gold standard' for radiation biodosimetry. Yet, DCA, as currently implemented, may be impractical for emergency response applications, especially when time is of the essence, owing to its labor-intensive and time-consuming nature. The growth of a primary lymphocyte culture for 48 h in vitro is required for DCA, and manual scoring of dicentric chromosomes (DCs) requires an additional 24-48 h, resulting in an overall processing time of 72-96 h for dose estimation. In order to improve this timing. we introduce a protocol that will detect the metaphase cells in a population of cells, and then will harvest only those metaphase cells. Our metaphase enrichment approach is based on fixed human lymphocytes incubated with monoclonal, anti-phosphorylated H3 histone (ser 10). Antibodies against this histone have been shown to be specific for mitotic cells. Colcemid is used to arrest the mitotic cells in metaphase. Following that, a flow-cytometric sorting apparatus isolates the mitotic fraction from a large population of cells, in a few minutes. These mitotic cells are then spread onto a slide and treated with our C-Banding procedure [Gonen et al. 2022], to visualize the centromeres with DAPI. This reduces the chemical processing time to ~2 h. This reduces the time required for the DCA and makes it practical for a much wider set of applications, such as emergency response following exposure of a large population to ionizing radiation.

Restoration of images captured by a staggered time delay and integration camera in the presence of mechanical vibrations.

Staggered time delay and integration (TDI) scanning image acquisition systems are usually employed in low signal-to-noise situations such as thermal imaging. Analysis and restoration of images acquired by thermal staggered TDI sensors in the presence of mechanical vibrations that may cause space-variant image distortions (severe geometric warps and blur) are studied. The relative motion at each location in the degraded image is identified from the image when a differential technique is used. This information is then used to reconstruct the image by a technique of projection onto convex sets. The main novelty is the implementation of such methods to scanned images (columnwise). Restorations are performed with simulated and real mechanically degraded thermal images.