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2.
Infect Immun ; 72(3): 1603-7, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14977967

RESUMO

Plasmodium falciparum-infected erythrocytes often sequester in the placenta of pregnant women, producing placental malaria, a condition that can compromise the health of the developing fetus. Scientists are hopeful that a vaccine can be developed to prevent this condition. Immunological mechanisms responsible for eliminating parasites from the placenta remain unclear, but antibodies to the carboxyl-terminal 19-kDa segment of the merozoite surface protein 1 (MSP1-19), the ring-infected erythrocyte surface antigen (RESA), and an erythrocyte-surface ligand that binds chondroitin sulfate A (CSA-L) have been implicated. In addition, antibodies to sporozoite and liver-stage antigens could reduce initial parasite burdens. This study sought to determine if antibodies to the circumsporozoite protein (CSP), liver-stage antigen 1 (LSA1), RESA, MSP1-19, or CSA-L correlated with either the absence of placental parasites or low placental parasitemias. Using a frequency-matched case-control study design, we compared antibody levels in women (gravidity 1 to 11) with and without placental malaria. Results showed that women who were antibody negative for MSP1-19 were at a higher risk of having placental malaria than women with antibodies (P < 0.007). Furthermore, an association between high levels of antibodies that blocked the binding of infected erythrocytes to CSA and low placental parasitemias was observed (P = 0.02). On the other hand, women with high antibody levels at term to CSP, LSA1, and RESA were more likely to have placental malaria than antibody-negative women. Since antibodies to MSP1-19 and CSA-L were associated with reduced placental malaria, both antigens show promise for inclusion in a vaccine for women of child-bearing age.


Assuntos
Anticorpos Antiprotozoários/sangue , Malária Falciparum/complicações , Malária Falciparum/imunologia , Placenta/parasitologia , Plasmodium falciparum/imunologia , Complicações Parasitárias na Gravidez/imunologia , Adulto , Sequência de Aminoácidos , Animais , Camarões , Estudos de Casos e Controles , Sulfatos de Condroitina/metabolismo , Eritrócitos/parasitologia , Feminino , Humanos , Técnicas In Vitro , Vacinas Antimaláricas/imunologia , Malária Falciparum/parasitologia , Proteína 1 de Superfície de Merozoito/genética , Proteína 1 de Superfície de Merozoito/metabolismo , Dados de Sequência Molecular , Plasmodium falciparum/genética , Plasmodium falciparum/isolamento & purificação , Gravidez , Complicações Parasitárias na Gravidez/parasitologia
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