RESUMO
Clenbuterol is a ß2 -adrenergic receptor agonist known to induce skeletal muscle hypertrophy and a slow-to-fast phenotypic shift. The aim of the present study was to test the effects of chronic clenbuterol treatment on contractile efficiency and explore the underlying mechanisms, i.e. the muscle contractile machinery and calcium-handling ability. Forty-three 6-week-old male Wistar rats were randomly allocated to one of six groups that were treated with either subcutaneous equimolar doses of clenbuterol (4 mg kg(-1) day(-1) ) or saline solution for 9, 14 or 21 days. In addition to the muscle hypertrophy, although an 89% increase in absolute maximal tetanic force (Po ) was noted, specific maximal tetanic force (sPo) was unchanged or even depressed in the slow twitch muscle of the clenbuterol-treated rats (P < 0.05). The fit of muscle contraction and relaxation force kinetics indicated that clenbuterol treatment significantly reduced the rate constant of force development and the slow and fast rate constants of relaxation in extensor digitorum longus muscle (P < 0.05), and only the fast rate constant of relaxation in soleus muscle (P < 0.05). Myofibrillar ATPase activity increased in both relaxed and activated conditions in soleus (P < 0.001), suggesting that the depressed specific tension was not due to the myosin head alteration itself. Moreover, action potential-elicited Ca(2+) transients in flexor digitorum brevis fibres (fast twitch fibres) from clenbuterol-treated animals demonstrated decreased amplitude after 14 days (-19%, P < 0.01) and 21 days (-25%, P < 0.01). In conclusion, we showed that chronic clenbuterol treatment reduces contractile efficiency, with altered contraction and relaxation kinetics, but without directly altering the contractile machinery. Lower Ca(2+) release during contraction could partially explain these deleterious effects.
Assuntos
Agonistas Adrenérgicos beta/farmacologia , Clembuterol/farmacologia , Contração Muscular/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Adenosina Trifosfatases/metabolismo , Animais , Cálcio/metabolismo , Hipertrofia/induzido quimicamente , Hipertrofia/metabolismo , Masculino , Músculo Esquelético/metabolismo , Doenças Musculares/induzido quimicamente , Doenças Musculares/metabolismo , Ratos , Ratos WistarRESUMO
Non-natural L-nucleoside analogues are increasingly used as therapeutic agents to treat cancer and viral infections. To be active, L-nucleosides need to be phosphorylated to their respective triphosphate metabolites. This stepwise phosphorylation relies on human enzymes capable of processing L-nucleoside enantiomers. We used crystallographic analysis to reveal the molecular basis for the low enantioselectivity and the broad specificity of human 3-phosphoglycerate kinase (hPGK), an enzyme responsible for the last step of phosphorylation of many nucleotide derivatives. Based on structures of hPGK in the absence of nucleotides, and bound to L and d forms of MgADP and MgCDP, we show that a non-specific hydrophobic clamp to the nucleotide base, as well as a water-filled cavity behind it, allows high flexibility in the interaction between PGK and the bases. This, combined with the dispensability of hydrogen bonds to the sugar moiety, and ionic interactions with the phosphate groups, results in the positioning of different nucleotides so to expose their diphosphate group in a position competent for catalysis. Since the third phosphorylation step is often rate limiting, our results are expected to alleviate in silico tailoring of L-type prodrugs to assure their efficient metabolic processing.
Assuntos
Fosfoglicerato Quinase/química , Pró-Fármacos/química , Difosfato de Adenosina/química , Antivirais/química , Antivirais/metabolismo , Cistina Difosfato/química , Desoxicitidina Quinase/química , Humanos , Ligantes , Modelos Moleculares , Núcleosídeo-Fosfato Quinase/química , Fosfoglicerato Quinase/metabolismo , Pró-Fármacos/metabolismo , Estrutura Terciária de Proteína , EstereoisomerismoRESUMO
Myosin VI is an unconventional myosin that may play a role in vesicular membrane traffic through actin rich regions of the cytoplasm in eukaryotic cells. In this study we have cloned and sequenced a cDNA encoding a chicken intestinal brush border myosin VI. Polyclonal antisera were raised to bacterially expressed fragments of this myosin VI. The affinity purified antibodies were highly specific for myosin VI by immunoblotting and immunoprecipitation and were used to study the localization of the protein by immunofluorescence and immunoelectron microscopy. It was found that in NRK and A431 cells, myosin VI was associated with both the Golgi complex and the leading, ruffling edge of the cell as well as being present in a cytosolic pool. In A431 cells in which cell surface ruffling was stimulated by EGF, myosin VI was phosphorylated and recruited into the newly formed ruffles along with ezrin and myosin V. In vitro experiments suggested that a p21-activated kinase (PAK) might be the kinase responsible for phosphorylation in the motor domain. These results strongly support a role for myosin VI in membrane traffic on secretory and endocytic pathways.
Assuntos
Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Fator de Crescimento Epidérmico/farmacologia , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Miosinas/metabolismo , Actinas/metabolismo , Sequência de Aminoácidos , Animais , Linhagem Celular , Membrana Celular/efeitos dos fármacos , Galinhas , Endocitose , Fibroblastos/metabolismo , Fibroblastos/ultraestrutura , Complexo de Golgi/efeitos dos fármacos , Humanos , Membranas Intracelulares/efeitos dos fármacos , Membranas Intracelulares/metabolismo , Membranas Intracelulares/ultraestrutura , Rim/metabolismo , Rim/ultraestrutura , Fígado/metabolismo , Fígado/ultraestrutura , Microscopia Imunoeletrônica , Microvilosidades/metabolismo , Microvilosidades/ultraestrutura , Dados de Sequência Molecular , Miosinas/química , Miosinas/genética , Fosforilação , Proteínas Serina-Treonina Quinases/metabolismo , Ratos , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Células Tumorais Cultivadas , Quinases Ativadas por p21RESUMO
We have mutated the lysine 128 of domain II of annexin I, which flanks a putative calcium-binding loop, into a glutamic acid residue. The properties of the mutated recombinant protein were compared to those of the wild-type recombinant protein. A change in the isotherm of calcium binding in the presence of lipids was observed. A slight decrease in the affinity for lipids was evident. When tested for the vesicle aggregation property, the mutation induced a change in lipid specificity; unlike the wild-type protein, the mutant protein aggregates vesicles containing phosphatidylserine plus phosphatidylethanolamine better than vesicles containing only phosphatidylserine. These experiments are in agreement with a model which suggests that a lipid molecule is inserted into the calcium-binding loop of annexin I and that the conserved lysine residue is involved in the specificity of annexins for anionic phospholipids.
Assuntos
Anexina A1/metabolismo , Cálcio/metabolismo , Fosfolipídeos/metabolismo , Sequência de Aminoácidos , Anexina A1/efeitos dos fármacos , Anexina A1/genética , Análise Mutacional de DNA , Lipossomos/metabolismo , Lisina/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Fosfatidiletanolaminas/farmacologia , Fosfatidilserinas/farmacologia , Ligação Proteica , Proteínas Recombinantes/metabolismo , Relação Estrutura-AtividadeRESUMO
The myofibril is a good model to study the ATPase of the muscle fibre. When myofibrillar ATPase reaction mixtures are quenched in acid, there is a burst of Pi formation, due to AM.ADP.Pi or Pi, as shown in the scheme: AM+ATP<-->A.M.ATP<-->AM.ADP.Pi<-->AM.ADP+Pi<-->AM+ADP. Therefore, in the steady state, either AM.ADP.Pi or AM.ADP or both predominate. To determine which, we studied the reaction using a Pi binding protein (from E. coli) labeled with a fluorophore such that it is specific and sensitive to free Pi [Brune, M. et al. (1994) Biochemistry 33, 8262-8271]. We show that the Pi bursts with myofibrillar ATPases (calcium-activated or not, or crosslinked) are due entirely to protein bound Pi. Thus, with myofibrillar ATPases the AM.ADP.Pi state predominates.
Assuntos
Adenosina Trifosfatases/metabolismo , Miofibrilas/metabolismo , Fosfatos/metabolismo , Actinas/metabolismo , Difosfato de Adenosina/metabolismo , Animais , Cálcio/metabolismo , Reagentes de Ligações Cruzadas , Relaxamento Muscular , Miofibrilas/enzimologia , Miosinas/metabolismo , Músculos Psoas , Coelhos , Fatores de TempoRESUMO
This study aimed to determine the changes in soleus myofibrillar ATPase (m-ATPase) activity and myosin heavy chain (MHC) isoform expression after endurance training and/or chronic hypoxic exposure. Dark Agouti rats were randomly divided into four groups: control, normoxic sedentary (N; n = 14), normoxic endurance trained (NT; n = 14), hypoxic sedentary (H; n = 10), and hypoxic endurance trained (HT; n = 14). Rats lived and trained in normoxia at 760 mmHg (N and NT) or hypobaric hypoxia at 550 mmHg (approximately 2,800 m) (H and HT). m-ATPase activity was measured by rapid flow quench technique; myosin subunits were analyzed with mono- and two-dimensional gel electrophoresis. Endurance training significantly increased m-ATPase (P < 0.01), although an increase in MHC-I content occurred (P < 0.01). In spite of slow-to-fast transitions in MHC isoform distribution in chronic hypoxia (P < 0.05) no increase in m-ATPase was observed. The rate constants of m-ATPase were 0.0350 +/- 0.0023 s(-1) and 0.047 +/- 0.0050 s(-1) for N and NT and 0.033 +/- 0.0021 s(-1) and 0.038 +/- 0.0032 s(-1) for H and HT. Thus, dissociation between variations in m-ATPase and changes in MHC isoform expression was observed. Changes in fraction of active myosin heads, in myosin light chain isoform (MLC) distribution or in MLC phosphorylation, could not explain the variations in m-ATPase. Myosin posttranslational modifications or changes in other myofibrillar proteins may therefore be responsible for the observed variations in m-ATPase activity.
Assuntos
Adenosina Trifosfatases/metabolismo , Hipóxia/fisiopatologia , Músculo Esquelético/enzimologia , Miofibrilas/enzimologia , Condicionamento Físico Animal/fisiologia , Resistência Física/fisiologia , Animais , Peso Corporal/fisiologia , Cálcio/metabolismo , Masculino , Cadeias Pesadas de Miosina/metabolismo , Ratos , Ratos EndogâmicosRESUMO
Traditionally, enzyme transient kinetics have been studied by the stopped-flow and rapid quench-flow (QF) methods. Whereas stopped-flow is the more convenient, it suffers from two weaknesses: optically silent systems cannot be studied, and when there is a signal it cannot always be assigned to a particular step in the reaction pathway. QF is a chemical sampling method; reaction mixtures are aged for a few milliseconds or longer, 'stopped' by a quenching agent and the product or the intermediate is measured by a specific analytical method. Here we show that by exploiting the array of current analytical methods and different quenching agents, the QF method is a key technique for identifying, and for characterising kinetically, intermediates in enzyme reaction pathways and for determining the order by which bonds are formed or cleaved by enzymes acting on polymer substrates such as DNA.
Assuntos
Enzimas/química , Enzimas/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Catálise , DNA/metabolismo , Cinética , Miosinas/química , Miosinas/metabolismo , Fosfotransferases/química , Fosfotransferases/metabolismoRESUMO
We studied the ATPase of shortening myofibrils at 4 degrees C by the rapid flow quench method. The progress curve has three phases: a P(i) burst, a fast linear phase kF of duration tB, and a deceleration to a slow kS. We propose that kF is the ATPase of myofibrils shortening under zero external load; at tB shortening and ATPase rates are reduced by passive resistance. The total ATP consumed during the rapid shortening is ATPc. Our purpose was to obtain information on the myofibrillar shortening velocity from their ATPase progress curves. We tested tB as an indicator of shortening velocity by determining the effects of different probes upon it and the other ATPase parameters. The dependence of tB upon the initial sarcomere length was linear, giving a shortening velocity close to that of muscle fibres (Vo). The Km of ATP was larger for tB than for kF, as found with fibers for Vo and their ATPase. ADP and 2,3-butanedione monoxime, but not P(i), inhibited tB to the same extent as Vo. The delta H for tB and Vo were similar. ATPc was independent of the sarcomere length, implying that the more the myofibrils shorten, the less ATP expended per myosin head per micron shortened. We propose that tB can be used as an indicator for myofibrillar shortening velocities.
Assuntos
Contração Muscular/fisiologia , Miofibrilas/fisiologia , Miosinas/metabolismo , Difosfato de Adenosina/farmacologia , Trifosfato de Adenosina/farmacologia , Animais , Fenômenos Biomecânicos , Fenômenos Biofísicos , Biofísica , Fosfatos de Dinucleosídeos/farmacologia , Inibidores Enzimáticos/farmacologia , Técnicas In Vitro , Cinética , Miofibrilas/efeitos dos fármacos , Miosinas/antagonistas & inibidores , Fosfatos/farmacologia , Coelhos , Sarcômeros/ultraestrutura , TemperaturaRESUMO
Our objective was to determine a good in vitro model for muscle fiber ATPase, and we compared the kinetics of Ca(2+)-activated myofibrils and cross-linked actoS1 in a buffer of physiological ionic strength. The myofibrils were cross-linked chemically to mimic the isometric condition of fibers or were un-cross-linked (the isotonic condition), and temperature perturbation was used to probe their ATPase mechanisms. At 4 degrees C, we have already shown that the kinetics of cross-linked actoS1 and myofibrils (cross-linked or not) are similar: there were large P(i) bursts and kcat values of about 1 s-1, close to that obtained with fibers [Herrmann, C., Sleep, J., Chaussepied, P., Travers, F. & Barman, T. (1993) Biochemistry 32, 7255-7263]. So, at 4 degrees C cross-linked actoS1 and myofibrils are equally good as models for fiber ATPase. At 20 degrees C, this similarity vanishes: progress curves with the myofibrils (cross-linked or not) had large P(i) bursts, but with cross-linked actoS1, bursts could not be discerned. This shows that at 20 degrees C the predominant steady-state intermediates are ATP complexes with actoS1 but are products complexes with the myofibrils, as with fibers [Ferenczi, M.A. (1986) Biophys. J. 50, 471-477]. Further, the kcat values were different: 15.5 s-1 with cross-linked actoS1, 8.3 s-1 for myofibrils, and 3.5 s-1 for cross-linked myofibrils. With fibers, kcat = 3.3 s-1. These results show that cross-linked myofibrillar ATPase is a good model for muscle fibers contracting isometrically.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Adenosina Trifosfatases/metabolismo , Músculos/enzimologia , Actomiosina/metabolismo , Trifosfato de Adenosina/metabolismo , Sítios de Ligação , Cálcio/metabolismo , Hidrólise , Cinética , Modelos Biológicos , TemperaturaRESUMO
The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP concentrations, the transient was biphasic, giving a k(fast)(max) of 230 s(-)(1) and a k(slow)(max) of 15 s(-)(1). The kinetics of the two phases were compared with those obtained by chemical sampling using [gamma-(32)P]ATP and quenching in acid (P(i) burst experiments: these give unambiguously the ATP cleavage kinetics), or cold Mg-ATP (cold ATP chase: ATP binding kinetics). k(slow) is due to ATP cleavage, as with S1. Interestingly, k(fast) is slower than the ATP binding kinetics. Instead, this constant appears to report ATP-induced cross-bridge detachment from actin because (1) it was identical to the fluorescence transient obtained on addition of ATP to pyrene-labeled myofibrils; (2) when the initial filament overlap in the myofibrils was decreased, the amplitude of the fast phase decreased; (3) there was no fluorescent enhancement upon the addition of ADP to myofibrils. This is different from the situation with S1 or actoS1 where there was also a fast fluorescent ATP-induced transient but whose kinetics were identical to those of the tight ATP binding. To increase the time resolution and to confirm our results, we also carried out transient kinetics in ethylene glycol and methanol. We interpret our results by a scheme in which a rapid equilibrium between attached (AM.ATP) and detached (M.ATP) states is modulated by the fraction of myosin heads in rigor (AM) during the time of experiment.
Assuntos
Adenosina Trifosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Miofibrilas/enzimologia , Músculos Psoas/enzimologia , Pirenos/química , Triptofano/química , Adenosina Trifosfatases/química , Animais , Soluções Tampão , Etilenoglicol/química , Cinética , Metanol/química , Ligação Proteica , Coelhos , Espectrometria de FluorescênciaRESUMO
We have exploited cryoenzymology, first, to probe the product release steps of myofibrillar ATPase under relaxing conditions and, second, to define the conditions for studying the contractile process in slow motion. Cryoenzymology implies perturbation by temperature and by the antifreeze added to allow for work at subzero temperatures. Here, we studied myofibrillar shortening and ATPases by the rapid quench flow method over a wide temperature range (-15 to 30 degrees C) in two antifreezes, 40% ethylene glycol and 20% methanol. The choice of solvent and temperature was dictated by the purpose of the experiment. Ethylene glycol (40%) is suitable for investigating the kinetics of the products release steps which is difficult in water. In this cryosolvent, the myofibrillar ATPase is not activated by Ca2+ nor is there shortening, except under special conditions, i.e., Ca2+ plus strong rigor bridges [Stehle, R., Lionne, C., Travers, F., and Barman, T. (1998) J. Muscl. Res. Cell Motil. 19, 381-392]. By the use of the glycol, we show that at low Ca2+ the kinetics of the ADP release are much faster with myofibrils than with S1. On the other hand, the kinetics of the Pi release were very similar for the two materials. Therefore, we suggest that, upon Ca2+ activation, only the Pi release kinetics are accelerated. In 20% methanol, in the presence of Ca2+, myofibrils shortened at temperatures above -2 degrees C but not below. At a given temperature above -2 degrees C, both the shortening and ATPase rates were reduced by the methanol. The temperature dependences of the myofibrillar ATPases (+/-Ca2+) converged with a decrease in temperature: at 20 degrees C, Ca2+ activated 30-fold, but at -15 degrees C, only about 5-fold. We suggest that studies in methanol may open the way for an investigation of muscle contraction in slow motion and, further, to obtain thermodynamic information on the internal forces involved in the shortening process.
Assuntos
Adenosina Trifosfatases/metabolismo , Contração Muscular , Miofibrilas/enzimologia , Difosfato de Adenosina/metabolismo , Animais , Ativação Enzimática , Etilenoglicol , Congelamento , Metanol , Relaxamento Muscular , Miosinas/metabolismo , Fosfatos/metabolismo , Músculos Psoas , Coelhos , Solventes , TemperaturaRESUMO
We have exploited solvent perturbation to probe the coupling of Ca2+ and rigor activation of the ATPase of myofibrils from rabbit psoas. Three techniques were used: overall myofibrillar ATPases by the rapid-flow quench method; kinetics of the interaction of ATP with myofibrils by fluorescence stopped-flow; and myofibrillar shortening by optical microscopy. Because of its extensive use with muscle systems, ranging from myosin subfragment-1 to muscle fibres, we chose 40% ethylene glycol as the relaxing agent. At 4 degrees C, the glycol had little effect on the myofibrillar ATPase at low [Ca2+], but at high [Ca2+] the activity was reduced 50-fold, close to the level found under relaxing conditions, and there was no shortening. However, the ATPase of chemically cross-linked myofibrils (permanently activated even without Ca2+) was reduced only 3-4-fold. The lesser reduction of the ATPase of permanently activated myofibrils was also observed in single turnover experiments in which activation occurs by a few heads in the rigor state activating the remaining heads. The addition of ADP, which also promotes strong head-thin filament interactions, also activated the ATPase but only in the presence of Ca2+. Further experiments revealed that in 40% ethylene glycol, Ca2+ does initiate shortening but only with the aid of strong interactions and at temperatures above 15 degrees C. This confirms that in the organized and intact myofibril, Ca2+ and rigor activation are coupled, as proposed previously for regulated actomyosin subfragment-1.
Assuntos
Adenosina Trifosfatases/metabolismo , Cálcio/metabolismo , Etilenoglicol/farmacologia , Miofibrilas/metabolismo , Músculos Psoas/metabolismo , Difosfato de Adenosina/farmacologia , Trifosfato de Adenosina/farmacologia , Animais , Cálcio/farmacologia , Reagentes de Ligações Cruzadas/metabolismo , Ativação Enzimática/efeitos dos fármacos , Contração Muscular/efeitos dos fármacos , Contração Muscular/fisiologia , Miofibrilas/efeitos dos fármacos , CoelhosRESUMO
We obtained the temperature dependences of the adenosine triphosphatase (ATPase) activities (calcium-activated and relaxed) of myofibrils from a slow muscle, which we compared with those from a fast muscle. We chose rabbit soleus and psoas because their myosin heavy chains are almost pure: isoforms I and IIX, respectively. The Arrhenius plots of the ATPases are linear (4-35 degrees C) with energies of activation for soleus myofibrils 155 kJ mol(-1) (activated) and 78 kJ mol(-1) (relaxed). With psoas myofibrils, the energies of activation were 71 kJ mol(-1) (activated) and 60 kJ mol(-1) (relaxed). When extrapolated to 42 degrees C the ATPase rates of the two types of myofibril were identical: 50 s(-1) (activated) and 0.23 s(-1) (relaxed). Whereas with psoas myofibrils the K(m) for adenosine triphosphate (activated ATPase) is relatively insensitive to temperature, that for soleus myofibrils increased from 0.3 microM at 4 degrees C to 66.5 microM at 35 degrees C. Our results illustrate the importance of temperature when comparing the mechanochemical coupling in different types of muscle. We discuss the problem of how to reconcile the similarity of the myofibrillar ATPase rates at physiological temperatures with their different mechanical properties.
Assuntos
Adenosina Trifosfatases/metabolismo , Contração Muscular/fisiologia , Fibras Musculares de Contração Rápida/citologia , Fibras Musculares de Contração Rápida/fisiologia , Fibras Musculares de Contração Lenta/citologia , Fibras Musculares de Contração Lenta/fisiologia , Sarcômeros/fisiologia , Sarcômeros/ultraestrutura , Animais , Cálcio/metabolismo , Células Dendríticas , Ativação Enzimática , Estabilidade Enzimática , Membro Posterior/fisiologia , Cinética , Músculo Esquelético/citologia , Músculo Esquelético/fisiologia , Miofibrilas/fisiologia , Miofibrilas/ultraestrutura , Coelhos , TemperaturaRESUMO
Myosin V plays an important role in membrane trafficking events. Its implication in the transport of pigment granules in melanocytes and synaptic vesicles in neurons is now well established. However, less is known about its function(s) in other cell types. Finding a common function is complicated by the diversity of myosin V expression in different tissues and organisms and by its association with different subcellular compartments. Here we show that myosin V is present in a variety of cells. Within the same cell type under different physiological conditions, we observed two main cellular locations for myosin V that were dependent on the dynamics of the plasma membrane: in cells with highly dynamic membranes, myosin V was specifically concentrated at the leading edge in membrane ruffles, whereas in cells with less dynamic membranes, myosin V was enriched around the microtubule-organizing center. The presence of myosin V in the leading ruffling edge of the cell was induced by growth factor stimulation and was dependent on the presence of a functional motor domain. Moreover, myosin V localization at the microtubule-organizing center was dependent on the integrity of the microtubules. In polarized epithelial cells (WIF-B), where the microtubule-organizing region is close to the actin-rich apical surface, one single pool of myosin V, sensitive to the integrity of both microtubules and actin filaments, was observed.
Assuntos
Citoesqueleto/metabolismo , Miosinas/metabolismo , Actinas/metabolismo , Anticorpos/imunologia , Centrossomo/metabolismo , Humanos , Fígado/metabolismo , Microscopia de Fluorescência , Microtúbulos/metabolismo , Miosinas/imunologia , Fator de Crescimento Derivado de Plaquetas/farmacologia , Transporte Proteico , Células Tumorais CultivadasRESUMO
All the functions of annexins in vitro as well as in vivo are mediated and probably regulated by calcium. We have used recombinant annexin I, synthesized by Escherichia coli, and we have performed site-directed mutagenesis. We have mutated the endonexin fold of domain 2 that binds calcium. Mutations were performed in this domain of the molecule because it perfectly matches the calcium binding consensus sequence. The two glycines of this fold were mutated into glutamic acid. The helix content and the stability of the mutants are identical to those of the wild-type, suggesting that the mutations did not drastically affect the structure of the protein. The two mutants showed modified calcium binding affinities. However, the calcium binding affinity of the G131E mutant was far more altered than that of the G129E mutant. Furthermore, other biochemical properties of these mutants were modified to different extents. The binding to phospholipid was not seriously affected, whereas the self-association was lost by the G131E mutant. In the same way, liposome aggregation is conserved, but modified, while the calcium affinity measured by equilibrium dialysis is dramatically altered.
Assuntos
Anexina A1/química , Anexina A1/metabolismo , Cálcio/metabolismo , Mutagênese Sítio-Dirigida , Anexina A1/genética , Sítios de Ligação , Cálcio/farmacologia , Diálise , Estabilidade de Medicamentos , Escherichia coli/genética , Glutamatos , Ácido Glutâmico , Glicina , Lipossomos/metabolismo , Modelos Moleculares , Estrutura Molecular , Dobramento de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Relação Estrutura-Atividade , TermodinâmicaRESUMO
Shortening and ATPase rates were measured in Ca2+-activated myofibrils from frog fast muscles in unloaded conditions at 4 degrees C. ATPase rates were determined using the phosphate-binding protein method (free phosphate) and quench flow (total phosphate). Shortening rates at near zero load (V0) were estimated by quenching reaction mixtures 50 ms to 10 s old at pH 3.5 and measuring sarcomere lengths under the optical microscope. As with the rabbit psoas myofibrils (C. Lionne, F. Travers, and T. Barman, 1996, Biophys. J. 70:887-895), the ATPase progress curves had three phases: a transient Pi burst, a fast linear phase (kF), and a deceleration to a slow phase (kS). Evidence is given that kF is the ATPase rate of shortening myofibrils. V0 is in good agreement with mechanical measurements in myofibrils and fibers. Under the same conditions and at saturation in ATP, V0 and kF are 2.4 microm half-sarcomere(-1) s(-1) and 4.6 s(-1), and their Km values are 33 and 200 microM, respectively. These parameters are higher than found with rabbit psoas myofibrils. The myofibrillar kF is higher than the fiber ATPase rates obtained previously in frog fast muscles but considerably lower than obtained in skinned fibers by the phosphate-binding protein method (Z. H. He, R. K. Chillingworth, M. Brune, J. E. T. Corrie, D. R. Trentham, M. R. Webb, and M. R. Ferenczi, 1997, J. Physiol. 50:125-148). We show that, with frog as with rabbit myofibrillar ATPase, phosphate release is the rate-limiting step.