RESUMO
The research is based on the analysis of 50 cases of anesthesia during reconstructive surgeries in children. The anesthesia was based on inhalation of sevolflurane and injection of fentanyl by the bolus infusion scheme. During the different stages of anesthesia the opioid concentration in plasma was measured. Analysis of hemodynamic parameters showed no statistically significant differences in changes in performance, indicating the steady flow of anesthesia. Infusion of fentanyl provided a smooth, gradual decrease in its concentration in plasma, while analgesia remained adequate. The combination of sevoflurane in a concentration of 1.3 MAC and infusion of fentanyl to a total dose 6 mkg/kg/h was effective in provisioning stable anesthesia in the given category of patients with surgical pathology.
Assuntos
Anestesia Geral/métodos , Anestésicos Inalatórios , Anestésicos Intravenosos/farmacocinética , Fentanila/farmacocinética , Éteres Metílicos , Adolescente , Anestésicos Inalatórios/administração & dosagem , Anestésicos Intravenosos/administração & dosagem , Anestésicos Intravenosos/sangue , Criança , Pré-Escolar , Quimioterapia Combinada , Fentanila/administração & dosagem , Fentanila/sangue , Hemodinâmica/efeitos dos fármacos , Humanos , Infusões Intravenosas , Injeções Intravenosas , Éteres Metílicos/administração & dosagem , Monitorização Intraoperatória , Sevoflurano , Resultado do TratamentoRESUMO
Conditions of isolation of opiates from tissues and analysis of the resultant extracts by immunochemical methods are discussed. The main characteristics of OPIATES U kits (Pharmatech) for polarization fluorescent immunoanalysis of cadaveric material (liver) are determined. Immunochemical methods for forensic chemical analysis for opiates are experimentally compared.
Assuntos
Imunoquímica/métodos , Fígado/química , Entorpecentes/análise , Medicina Legal/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Reprodutibilidade dos TestesRESUMO
The possibility of using immunochemical methods in general forensic chemical analysis of cadaveric material for narcotics is demonstrated. Conditions of isolation of 1,4-benzodiasepines from tissues and analysis of the resultant tissue extracts by polarization fluoroimmunoassay (PFIA) and solid-phase enzyme immunoassay are described. Time course of concentrations in tissue extracts stored for a long time has been studied by PFIA. Characteristics of Benzodiazepines Serum kits (Abbott) for PFIA of liver extracts are determined.