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1.
J Water Health ; 21(9): 1209-1227, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37756190

RESUMO

By community intervention in 14 non-disinfecting municipal water systems, we quantified sporadic acute gastrointestinal illness (AGI) attributable to groundwater. Ultraviolet (UV) disinfection was installed on all supply wells of intervention communities. In control communities, residents continued to drink non-disinfected groundwater. Intervention and control communities switched treatments by moving UV disinfection units at the study midpoint (crossover design). Study participants (n = 1,659) completed weekly health diaries during four 12-week surveillance periods. Water supply wells were analyzed monthly for enteric pathogenic viruses. Using the crossover design, groundwater-borne AGI was not observed. However, virus types and quantity in supply wells changed through the study, suggesting that exposure was not constant. Alternatively, we compared AGI incidence between intervention and control communities within the same surveillance period. During Period 1, norovirus contaminated wells and AGI attributable risk from well water was 19% (95% CI, -4%, 36%) for children <5 years and 15% (95% CI, -9%, 33%) for adults. During Period 3, echovirus 11 contaminated wells and UV disinfection slightly reduced AGI in adults. Estimates of AGI attributable risks from drinking non-disinfected groundwater were highly variable, but appeared greatest during times when supply wells were contaminated with specific AGI-etiologic viruses.


Assuntos
Água Potável , Água Subterrânea , Adulto , Criança , Humanos , Abastecimento de Água , Desinfecção , Enterovirus Humano B
2.
Environ Res ; 210: 112967, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35189100

RESUMO

Wastewater-based epidemiology offers a time- and cost-effective way to monitor SARS-CoV-2 spread in communities and therefore represents a complement to clinical testing. WBE applicability has been demonstrated in a number of cases over short-term periods as a method for tracking the prevalence of SARS-CoV-2 and an early-warning tool for predicting outbreaks in the population. This study reports SARS-CoV-2 viral loads from wastewater treatment plants (WWTPs) and hospitals over a 6-month period (June to December 2020). Results show that the overall range of viral load in positive tested samples was between 1.2 × 103 and 3.5 × 106 gene copies/l, unveiling that secondary-treated wastewaters mirrored the viral load of influents. The interpretation suggests that the viral titers found in three out of four WWTPs were associated to clinical COVID-19 surveillance indicators preceding 2-7 days the rise of reported clinical cases. The median wastewater detection rate of SARS-CoV-2 was one out of 14,300 reported new cases. Preliminary model estimates of prevalence ranged from 0.02 to 4.6% for the studied period. This comprehensive statistical and epidemiological analysis demonstrates that the applied wastewater-based approach to COVID-19 surveillance is in general consistent and feasible, although there is room for improvements.


Assuntos
COVID-19 , SARS-CoV-2 , COVID-19/diagnóstico , COVID-19/epidemiologia , Humanos , México/epidemiologia , RNA Viral/genética , SARS-CoV-2/genética , Águas Residuárias
3.
J Environ Manage ; 309: 114698, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35183939

RESUMO

A literature review was carried out to analyze the current status of microplastic research in Latin America and the Caribbean (LAC). Specifically, this work focused on publications pertaining to (1) occurrence and distribution of microplastics in the environment, including water, sediments, and soil and (2) the environmental impact of MPs, particularly their presence and effects on aquatic and terrestrial organisms. The review included peer-reviewed articles from Scopus, Science Direct, Web of Science, Google Scholar and two iberoamerican open access databases (Redalyc and SciELO). It was found that LAC has only contributed to 5% of the global scientific output on microplastics, and overall the highest contributor within the region was Brazil (52%), followed by Chile (16%) and Mexico (13%). An additional section analyzing the barriers to conducting microplastic research in LAC and their exacerbation by the current COVID-19 pandemic was included to provide additional context behind the relatively low scientific production and improve recommendations encouraging research in this region.


Assuntos
COVID-19 , Microplásticos , Região do Caribe/epidemiologia , Humanos , América Latina/epidemiologia , Pandemias , Plásticos , SARS-CoV-2
4.
Ecotoxicol Environ Saf ; 142: 157-163, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28407501

RESUMO

We previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (PAHs) increased sensitivity of rainbow trout (Oncorhynchus mykiss) to subsequent challenge with Aeromonas salmonicida, the causative agent of furunculosis. In this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary PAH exposure and pathogen challenge. Rainbow trout were fed a mixture of ten high molecular weight PAHs at an environmentally relevant concentration (7.82µg PAH mixture/g fish/day) or control diet for 50 days. After 50 days of PAH exposure, fish were challenged with either Aeromonas salmonicida at a lethal concentration 30 (LC30) or growth media without the pathogen (mock challenge). Head kidneys were collected 2, 4, 10 and 20 days after challenge and gene expression (q<0.05) was evaluated among treatments. In animals fed the PAH contaminated diet, we observed down-regulation of expression for innate immune system genes in pathways (p<0.05) for the terminal steps of the complement cascade (complement component C6) and other bacteriolytic processes (lysozyme type II) potentially underlying increased disease susceptibility after pathogen challenge. Increased expression of genes associated with hemorrhage/tissue remodeling/inflammation pathways (p<0.05) was likely related to more severe head kidney damage due to infection in PAH-fed compared to control-fed fish. This study is the first to evaluate transcriptional signatures associated with the impact of chronic exposure to an environmentally relevant mixture of PAHs in disease susceptibility and immunity.


Assuntos
Aeromonas salmonicida/patogenicidade , Rim Cefálico/imunologia , Imunidade Inata/efeitos dos fármacos , Oncorhynchus mykiss/microbiologia , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Transcrição Gênica/efeitos dos fármacos , Animais , Regulação para Baixo , Imunidade Inata/genética , Muramidase/metabolismo , Oncorhynchus mykiss/imunologia , Oncorhynchus mykiss/metabolismo
5.
Bioresour Technol ; 395: 130270, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38158093

RESUMO

Microalgal-bacterial aggregates (MBAs) have recently attracted significant attention as a potential replacement for conventional, suspended-growth wastewater treatment processes. This article evaluates MBAs for full-scale implementation from the perspective of oxygen supply, land use, and energy savings. The results suggest that under ideal conditions, photosynthesis and atmospheric diffusion would provide at most only 2.7% of the oxygen demand in a conventionally designed, nitrifying activated sludge process, which is equivalent to approximately 1.5% of typical treatment plant-wide energy requirements. The results also suggest that a wastewater treatment process using MBAs and relying on solar photosynthesis and atmospheric diffusion for oxygen would have nearly the same footprint as an equivalent well-mixed wastewater treatment pond. While photosynthesis and passive atmospheric diffusion are capable of providing significant oxygen for suspended-growth wastewater treatment processes, the tradeoffs between footprint requirements and energy savings should be carefully considered.


Assuntos
Microalgas , Purificação da Água , Águas Residuárias , Esgotos/microbiologia , Bactérias , Purificação da Água/métodos , Oxigênio , Eliminação de Resíduos Líquidos , Reatores Biológicos
6.
Sci Total Environ ; 944: 173735, 2024 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-38857803

RESUMO

Environmental concerns about microplastics (MPs) have motivated research of their sources, occurrence, and fate in aquatic and soil ecosystems. To mitigate the environmental impact of MPs, biodegradable plastics are designed to naturally decompose, thus reducing the amount of environmental plastic contamination. However, the environmental fate of biodegradable plastics and the products of their incomplete biodegradation, especially micro-biodegradable plastics (MBPs), remains largely unexplored. This comprehensive review aims to assess the risks of unintended consequences associated with the introduction of biodegradable plastics into the environment, namely, whether the incomplete mineralization of biodegradable plastics could enhance the risk of MBPs formation and thus, exacerbate the problem of their environmental dispersion, representing a potentially additional environmental hazard due to their presumed ecotoxicity. Initial evidence points towards the potential for incomplete mineralization of biodegradable plastics under both controlled and uncontrolled conditions. Rapid degradation of PLA in thermophilic industrial composting contrasts with the degradation below 50 % of other biodegradables, suggesting MBPs released into the environment through compost. Moreover, degradation rates of <60 % in anaerobic digestion for polymers other than PLA and PHAs suggest a heightened risk of MBPs in digestate, risking their spread into soil and water. This could increase MBPs and adsorbed pollutants' mobilization. The exact behavior and impacts of additive leachates from faster-degrading plastics remain largely unknown. Thus, assessing the environmental fate and impacts of MBPs-laden by-products like compost or digestate is crucial. Moreover, the ecotoxicological consequences of shifting from conventional plastics to biodegradable ones are highly uncertain, as there is insufficient evidence to claim that MBPs have a milder effect on ecosystem health. Indeed, literature shows that the impact may be worse depending on the exposed species, polymer type, and the ecosystem complexity.


Assuntos
Plásticos Biodegradáveis , Biodegradação Ambiental , Microplásticos , Monitoramento Ambiental , Poluentes Químicos da Água/análise , Poluentes do Solo/análise , Plásticos
7.
Sci Total Environ ; 927: 172216, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38583614

RESUMO

Antibiotic resistance genes (ARGs) are a major threat to human and environmental health. This study investigated the occurrence and distribution of ARGs in Lake Cajititlán, a hypereutrophic subtropical lake in Mexico contaminated by anthropogenic sources (urban wastewater and runoff from crop and livestock production). ARGs (a total of 475 genes) were detected in 22 bacterial genera, with Pseudomonas (144 genes), Stenotrophomonas (88 genes), Mycobacterium (54 genes), and Rhodococcus (27 genes) displaying the highest frequencies of ARGs. Among these, Pseudomonas aeruginosa and Stenotrophomonas maltophilia showed the highest number of ARGs. The results revealed a diverse array of ARGs, including resistance to macrolides (11.55 %), aminoglycosides (8.22 %), glycopeptides (6.22 %), tetracyclines (4 %), sulfonamides (4 %), carbapenems (1.11 %), phenicols (0.88 %), fluoroquinolones (0.44 %), and lincosamides (0.22 %). The most frequently observed ARGs were associated with multidrug resistance (63.33 %), with MexF (42 genes), MexW (36 genes), smeD (31 genes), mtrA (25 genes), and KHM-1 (22 genes) being the most common. Lake Cajititlán is a recreational area for swimming, fishing, and boating, while also supporting irrigation for agriculture and potentially acting as a drinking water source for some communities. This raises concerns about the potential for exposure to antibiotic-resistant bacteria through these activities. The presence of ARGs in Lake Cajititlán poses a significant threat to both human and environmental health. Developing strategies to mitigate the risks of antibiotic resistance, including improving wastewater treatment, and promoting strategic antibiotic use and disposal, is crucial. This study represents a significant advancement in the understanding of antibiotic resistance dynamics in a hypereutrophic subtropical lake in a developing country, providing valuable insights for the scientific community and policymakers.


Assuntos
Resistência Microbiana a Medicamentos , Monitoramento Ambiental , Lagos , Lagos/microbiologia , Resistência Microbiana a Medicamentos/genética , México , Antibacterianos/farmacologia , Metagenômica , Genes Bacterianos , Farmacorresistência Bacteriana/genética , Águas Residuárias/microbiologia , Bactérias/efeitos dos fármacos , Bactérias/genética , Poluentes Químicos da Água/análise
8.
Sci Total Environ ; 905: 166863, 2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-37690767

RESUMO

Nitrate contamination in groundwater poses a significant threat to water quality and public health, especially in regions with limited data availability. This study addresses this challenge by employing machine learning (ML) techniques to predict nitrate (NO3--N) concentrations in Mexico's groundwater. Four ML algorithms-Extreme Gradient Boosting (XGB), Boosted Regression Trees (BRT), Random Forest (RF), and Support Vector Machines (SVM)-were executed to model NO3--N concentrations across the country. Despite data limitations, the ML models achieved robust predictive performances. XGB and BRT algorithms demonstrated superior accuracy (0.80 and 0.78, respectively). Notably, this was achieved using ∼10 times less information than previous large-scale assessments. The novelty lies in the first-ever implementation of the 'Support Points-based Split Approach' during data pre-processing. The models considered initially 68 covariates and identified 13-19 significant predictors of NO3--N concentration spanning from climate, geomorphology, soil, hydrogeology, and human factors. Rainfall, elevation, and slope emerged as key predictors. A validation incorporated nationwide waste disposal sites, yielding an encouraging correlation. Spatial risk mapping unveiled significant pollution hotspots across Mexico. Regions with elevated NO3--N concentrations (>10 mg/L) were identified, particularly in the north-central and northeast parts of the country, associated with agricultural and industrial activities. Approximately 21 million people, accounting for 10 % of Mexico's population, are potentially exposed to elevated NO3--N levels in groundwater. Moreover, the NO3--N hotspots align with reported NO3--N health implications such as gastric and colorectal cancer. This study not only demonstrates the potential of ML in data-scarce regions but also offers actionable insights for policy and management strategies. Our research underscores the urgency of implementing sustainable agricultural practices and comprehensive domestic waste management measures to mitigate NO3--N contamination. Moreover, it advocates for the establishment of effective policies based on real-time monitoring and collaboration among stakeholders.


Assuntos
Água Subterrânea , Poluentes Químicos da Água , Humanos , Nitratos/análise , Poluentes Químicos da Água/análise , Monitoramento Ambiental/métodos , Compostos Orgânicos , Qualidade da Água , Abastecimento de Água
9.
Environ Sci Technol ; 46(17): 9299-307, 2012 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-22839570

RESUMO

Acute gastrointestinal illness (AGI) resulting from pathogens directly entering the piping of drinking water distribution systems is insufficiently understood. Here, we estimate AGI incidence from virus intrusions into the distribution systems of 14 nondisinfecting, groundwater-source, community water systems. Water samples for virus quantification were collected monthly at wells and households during four 12-week periods in 2006-2007. Ultraviolet (UV) disinfection was installed on the communities' wellheads during one study year; UV was absent the other year. UV was intended to eliminate virus contributions from the wells and without residual disinfectant present in these systems, any increase in virus concentration downstream at household taps represented virus contributions from the distribution system (Approach 1). During no-UV periods, distribution system viruses were estimated by the difference between well water and household tap virus concentrations (Approach 2). For both approaches, a Monte Carlo risk assessment framework was used to estimate AGI risk from distribution systems using study-specific exposure-response relationships. Depending on the exposure-response relationship selected, AGI risk from the distribution systems was 0.0180-0.0661 and 0.001-0.1047 episodes/person-year estimated by Approaches 1 and 2, respectively. These values represented 0.1-4.9% of AGI risk from all exposure routes, and 1.6-67.8% of risk related to drinking water exposure. Virus intrusions into nondisinfected drinking water distribution systems can contribute to sporadic AGI.


Assuntos
Água Potável/efeitos adversos , Água Potável/virologia , Gastroenteropatias/etiologia , Gastroenteropatias/virologia , Viroses/etiologia , Adulto , Criança , Desinfecção/métodos , Trato Gastrointestinal/virologia , Humanos , Incidência , Medição de Risco , Raios Ultravioleta , Viroses/virologia , Purificação da Água/métodos
10.
Environ Sci Technol ; 46(11): 6081-7, 2012 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-22587496

RESUMO

The health condition of out-migrating juvenile salmonids can influence migration success. Physical damage, pathogenic infection, contaminant exposure, and immune system status can affect survival probability. The present study is part of a wider investigation of out-migration success in juvenile steelhead (Oncorhynchus mykiss) and focuses on the application of molecular profiling to assess sublethal effects of environmental stressors in field-collected fish. We used a suite of genes in O. mykiss to specifically assess responses that could be directly related to steelhead health condition during out-migration. These biomarkers were used on juvenile steelhead captured in the Snake River, a tributary of the Columbia River, in Washington, USA, and were applied on gill and anterior head kidney tissue to assess immune system responses, pathogen-defense (NRAMP, Mx, CXC), general stress (HSP70), metal-binding (metallothionein-A), and xenobiotic metabolism (Cyp1a1) utilizing quantitative polymerase chain reaction (PCR) technology. Upon capture, fish were ranked according to visual external physical conditions into good, fair, poor, and bad categories; gills and kidney tissues were then dissected and preserved for gene analyses. Transcription responses were tissue-specific for gill and anterior head kidney with less significant responses in gill tissue than in kidney. Significant differences between the condition ranks were attributed to NRAMP, MX, CXC, and Cyp1a1 responses. Gene profiling correlated gene expression with pathogen presence, and results indicated that gene profiling can be a useful tool for identifying specific pathogen types responsible for disease. Principal component analysis (PCA) further correlated these responses with specific health condition categories, strongly differentiating good, poor, and bad condition ranks. We conclude that molecular profiling is an informative and useful tool that could be applied to indicate and monitor numerous population-level parameters of management interest.


Assuntos
Saúde Ambiental , Monitoramento Ambiental , Perfilação da Expressão Gênica , Saúde , Oncorhynchus mykiss/genética , Rios , Transcrição Gênica , Animais , Análise por Conglomerados , Análise de Componente Principal , Washington
11.
Sci Total Environ ; 806(Pt 1): 150386, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34560458

RESUMO

The Caplina/Concordia transboundary coastal aquifer system, located in the Atacama Desert, is the primary source of water supply for domestic use and irrigation for La Yarada-Los Palos (Peru) and Concordia (Chile) agriculture districts, and to a lesser extent, for Tacna province public supply use (Peru). Despite the scarce amount of rainfall (<20 mm/year) in the area and the limited recharge coming from the Andean highlands, this transboundary aquifer system has been overexploited mainly for agriculture since before the 2000s on the Peruvian side. Consequently, this has caused groundwater depletion and seawater intrusion. In this study, comprehensive hydrogeological information was integrated to understand the aquifer system's behavior and the effects to which it has been subjected to groundwater overexploitation. To that end, a 3D hydrogeological framework was developed using the LEAPFROG software and a constant-density groundwater flow model with equivalent heads was generated in FEFLOW software, which was adjusted with Monte Carlo analysis and conventional automated calibration. Finally, eight scenarios, considering various water resource management options proposed by the authority and potential climatic trends (CMIP6), were simulated from 2020 to 2040. The results showed that between 2002 and 2020, the increase in the seawater wedge and the average groundwater level decline were 216 hm3/year and 7 m, respectively. It is expected that the depletion will continue with a groundwater level decline between 5 and 8 m and an increase in the seawater wedge between 1120 hm3/year and 1175 hm3/year for the forecast period. The study concludes that the aquifer system will remain unsustainable for the next 20 years, regardless of the selected scenarios, and suggests that any mitigation measure requires the participation of stakeholders from Peru, Chile, and Bolivia.


Assuntos
Água Subterrânea , Chile , Peru , Água do Mar , Abastecimento de Água
12.
J Water Health ; 9(4): 799-812, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22048438

RESUMO

We tested the association of common events in drinking water distribution systems with contamination of household tap water with human enteric viruses. Viruses were enumerated by qPCR in the tap water of 14 municipal systems that use non-disinfected groundwater. Ultraviolet disinfection was installed at all active wellheads to reduce virus contributions from groundwater to the distribution systems. As no residual disinfectant was added to the water, any increase in virus levels measured downstream at household taps would be indicative of distribution system intrusions. Utility operators reported events through written questionnaires. Virus outcome measures were related to distribution system events using binomial and gamma regression. Virus concentrations were elevated in the wells, reduced or eliminated by ultraviolet disinfection, and elevated again in distribution systems, showing that viruses were, indeed, directly entering the systems. Pipe installation was significantly associated with higher virus levels, whereas hydrant flushing was significantly associated with lower virus levels. Weak positive associations were observed for water tower maintenance, valve exercising, and cutting open a water main. Coliform bacteria detections from routine monitoring were not associated with viruses. Understanding when distribution systems are most vulnerable to virus contamination, and taking precautionary measures, will ensure delivery of safe drinking water.


Assuntos
Enterovirus/isolamento & purificação , Microbiologia da Água , Abastecimento de Água/normas , Humanos , Engenharia Sanitária , Wisconsin
13.
Water Environ Res ; 83(5): 461-9, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21657197

RESUMO

The purpose of this research was to develop a better understanding of the dynamic effects of anaerobic hydraulic retention time (HRT) on both enhanced biological phosphorus removal (EBPR) performance and enrichment of phosphorus accumulating organisms (PAOs). The research was conducted using laboratory-scale sequencing batch reactors inoculated with mixed microbial consortia and fed real wastewater. Exposing microorganisms to extended anaerobic HRTs is not recommended for EBPR configured systems. In this research, however, longer anaerobic exposure did not negatively affect performance even if volatile fatty acids were depleted. Further, extended anaerobic HRTs may positively affect phosphorus removal through enhanced aerobic uptake. The EBPR consortia also appear to maintain reserve energetic capacity in the form of polyphosphate that can be used to survive and grow under variable operational and environmental conditions. Finally, the tested EBPR systems yield mixed microbial consortia enriched with PAOs (specifically Candidatus Accumulibacter phosphatis) at approximately 7.1 to 21.6% of the total population.


Assuntos
Bactérias Anaeróbias/fisiologia , Fósforo/química , Esgotos/microbiologia , Purificação da Água , Biodegradação Ambiental , Reatores Biológicos , Consórcios Microbianos
14.
Water Environ Res ; 83(1): 36-43, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21291026

RESUMO

The influence of four main process parameters--solids retention time (SRT), hydraulic retention time (HRT), anoxic-oxic cycling, and carbon-to-nitrogen ratio (C/N ratio)--on poly(hydroxyalkanoate) (PHA) production, while treating brewery wastewater, was studied. Two sets of two-level, three-factor experimental designs were implemented to (1) determine the effects and interactions among process parameters, (2) assess their significance to PHA production, and (3) approximate optimal operational conditions. The HRT and SRT were found to be the crucial operational parameters affecting PHA production. The highest PHA content of 55% (on a cell-weight basis) was produced at a 4-day HRT and 4-day SRT, whereas a maximum PHA concentration of 907 mg/L was obtained at a 2-day HRT and 12-day SRT. The effect of anoxic conditions on PHA production was insignificant. The C/N ratio played a more important role in the PHA concentration in the system than in the PHA content in the biomass.


Assuntos
Indústria Alimentícia , Resíduos Industriais , Poli-Hidroxialcanoatos/metabolismo , Eliminação de Resíduos Líquidos/métodos , Cerveja , Monitoramento Ambiental , Poli-Hidroxialcanoatos/química
15.
Water Environ Res ; 83(4): 313-25, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21553587

RESUMO

In disinfection systems, incomplete penetration of chlorine into effluent wastewater particles can result in a residual population of viable microorganisms. In this work, a combined experimental and numerical approach was used to quantify inactivation of microorganisms in effluent particles and identify combinations of particle removal and chlorine dose that would result in a reduction of occluded microorganisms for six full-scale facilities in the United States with different nitrification levels. The results reveal that combined chlorine is more effective for inactivating occluded microorganisms than free chlorine; model calibration results suggest that free chlorine is less effective because it is more reactive. However, nitrified effluents appear to have lower effluent particle concentrations, and decreases in particle concentrations significantly reduce the chlorine required. Additionally, in disinfection systems that are designed and operated based on inactivation of indicator organisms, the chlorine dose may be insufficient to inactivate occluded pathogens to levels consistent with current regulations.


Assuntos
Desinfecção/métodos , Filtração/métodos , Modelos Teóricos , Microbiologia da Água , Poluentes Químicos da Água/metabolismo , Calibragem , Tamanho da Partícula
16.
Water Environ Res ; 82(3): 216-26, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20369565

RESUMO

The purpose of the research presented herein was to evaluate the effects of solids residence time (SRT) and organic acid augmentation on biological phosphorus removal (BPR), with a focus on how these operational variables affect key metabolisms and the distribution of the microbial population. Using laboratory-scale sequencing batch reactors seeded with a mixed microbial consortium and fed real wastewater, we observed that longer SRTs can improve BPR performance; organic acid augmentation can stabilize BPR, but it is not necessary for process success; and higher volatile suspended solids concentrations correlate with improved phosphorus removal. The results also suggest that organic acids may not be critical in driving anaerobic phosphorus release, but in driving aerobic growth. Finally, given an observed population similarity across all tested bioreactors, BPR variability appears to be less influenced by the presence of specific microbes and more affected by the induction of critical metabolisms.


Assuntos
Reatores Biológicos , Ácidos Graxos Voláteis/química , Fósforo/isolamento & purificação , Esgotos/química , Gerenciamento de Resíduos/métodos , Oxigênio/química , Esgotos/microbiologia
17.
Risk Anal ; 29(2): 257-66, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19144071

RESUMO

The overall goal of the study reported herein was to use techniques in the field of risk assessment (specifically a state-space population dynamic model of disease transmission within recreational waters) to explore the relative significance of (1) active shedding of microorganisms from bathers themselves, and (2) the type and concentration of etiological agent on the observed heterogeneity of the incidence of illness in epidemiological studies that have been used to develop ambient water quality criteria. The etiological agent and corresponding dose ingested during recreational contact was found to significantly impact the observed incidence of illness in an epidemiological study conducted in recreational water. In addition, the observed incidence of illness was found not to necessarily reflect background concentrations of indicator organisms, but rather microorganisms shed during recreational contact. Future revisions to ambient water quality criteria should address the etiological agent, dose, and the significance of microbial shedding relative to background concentrations of pathogens and indicator organisms in addition to the incidence of illness and concentration of indicator organisms. Without a quantitative assessment of these additional variables, study findings may potentially be site specific and not representative of the health risks associated with specific indicator concentrations in all recreational waters.


Assuntos
Praias/normas , Medição de Risco/métodos , Microbiologia da Água/normas , Abastecimento de Água/normas , Algoritmos , Doenças Transmissíveis/epidemiologia , Monitoramento Ambiental/normas , Estudos Epidemiológicos , Monitoramento Epidemiológico , Humanos , Modelos Estatísticos , Ensaios Clínicos Controlados Aleatórios como Assunto , Água/análise
18.
Appl Environ Microbiol ; 74(10): 2990-6, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18359827

RESUMO

Available filtration methods to concentrate waterborne viruses are either too costly for studies requiring large numbers of samples, limited to small sample volumes, or not very portable for routine field applications. Sodocalcic glass wool filtration is a cost-effective and easy-to-use method to retain viruses, but its efficiency and reliability are not adequately understood. This study evaluated glass wool filter performance to concentrate the four viruses on the U.S. Environmental Protection Agency contaminant candidate list, i.e., coxsackievirus, echovirus, norovirus, and adenovirus, as well as poliovirus. Total virus numbers recovered were measured by quantitative reverse transcription-PCR (qRT-PCR); infectious polioviruses were quantified by integrated cell culture (ICC)-qRT-PCR. Recovery efficiencies averaged 70% for poliovirus, 14% for coxsackievirus B5, 19% for echovirus 18, 21% for adenovirus 41, and 29% for norovirus. Virus strain and water matrix affected recovery, with significant interaction between the two variables. Optimal recovery was obtained at pH 6.5. No evidence was found that water volume, filtration rate, and number of viruses seeded influenced recovery. The method was successful in detecting indigenous viruses in municipal wells in Wisconsin. Long-term continuous filtration retained viruses sufficiently for their detection for up to 16 days after seeding for qRT-PCR and up to 30 days for ICC-qRT-PCR. Glass wool filtration is suitable for large-volume samples (1,000 liters) collected at high filtration rates (4 liters min(-1)), and its low cost makes it advantageous for studies requiring large numbers of samples.


Assuntos
Adenoviridae/isolamento & purificação , Enterovirus/isolamento & purificação , Filtração/métodos , Água Doce/virologia , Norovirus/isolamento & purificação , DNA Viral/genética , Vidro , Concentração de Íons de Hidrogênio , Reação em Cadeia da Polimerase/métodos , RNA Viral/genética , Sensibilidade e Especificidade , Cultura de Vírus , Wisconsin
19.
Bioresour Technol ; 99(7): 2680-6, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-17574844

RESUMO

Previous research has demonstrated that production of natural fiber reinforced thermoplastic composites (NFRTCs) utilizing bacterially-derived pure polyhydroxybutyrate (PHB) does not yield a product that is cost competitive with synthetic plastic-based NFRTCs. Moreover, the commercial production of pure PHB is not without environmental impacts. To address these issues, we integrated unpurified PHB in NFRTC construction, thereby eliminating a significant energy and cost sink (ca. 30-40%) while concurrently yielding a fully biologically based commodity. PHB-rich biomass synthesized with the microorganism Azotobacter vinelandii UWD was utilized to manufacture NFRTCs with wood flour. Resulting composites exhibited statistically similar bending strength properties despite relatively different PHB contents. Moreover, the presence of microbial cell debris allowed for NFRTC processing at significantly reduced polymer content, relative to pure PHB-based NFRTCs. Results further indicate that current commercial PHB production yields are sufficiently high to produce composites comparable to those manufactured with purified PHB.


Assuntos
Biomassa , Hidroxibutiratos/química , Plásticos , Polímeros/química
20.
Water Environ Res ; 80(4): 367-72, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18536488

RESUMO

Polyhydroxyalkanoate (PHA) production was achieved using tomato cannery waste coupled with a mixed microbial culture during wastewater treatment. The two-stage PHA production process comprised a sequencing batch reactor (SBR), operating under a periodic feast-famine regime, to accomplish simultaneously wastewater treatment and selection of PHA-accumulating microbes, followed by a batch reactor for the production of PHA-rich biomass. The SBRs were efficient at removing soluble carbon (84%), ammonia (100%), and phosphorus (76%). Meanwhile, PHA-accumulating microbes were enriched under the SBR operating conditions, and PHA content on a cell-weight basis was within the range 7 to 11% in nonfiltered wastewater and 2 to 8% in filtered wastewater. Subsequently, batch studies were implemented with varying loading rates, ranging from 0.4 to 3.2 food-to-microorganism ratios. A maximum 20% PHA content on a cell-weight basis was obtained. Based on the experimental results, a PHA biosynthesis-degradation kinetic model was developed to (1) aid in the design of a pilot- or full-scale PHA production process coupled with wastewater treatment and (2) determine optimal conditions for harvest of PHA-rich biomass.


Assuntos
Reatores Biológicos , Resíduos Industriais/análise , Poli-Hidroxialcanoatos/biossíntese , Eliminação de Resíduos Líquidos/métodos , Bactérias/metabolismo , Reatores Biológicos/microbiologia , Cinética , Solanum lycopersicum , Modelos Químicos , Oxigênio/metabolismo
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