RESUMO
The ancestral gamete fusion protein, HAP2, catalyzes sperm-egg fusion in a broad range of taxa dating to the last eukaryotic common ancestor. Remarkably, HAP2 orthologs are structurally related to the class II fusogens of modern-day viruses, and recent studies make clear that these proteins utilize similar mechanisms to achieve membrane merger. To identify factors that may regulate HAP2 activity, we screened mutants of the ciliate Tetrahymena thermophila for behaviors that mimic Δhap2 knockout phenotypes in this species. Using this approach, we identified two new genes, GFU1 and GFU2, whose products are necessary for the formation of membrane pores during fertilization and show that the product of a third gene, namely ZFR1, may be involved in pore maintenance and/or expansion. Finally, we propose a model that explains cooperativity between the fusion machinery on apposed membranes of mating cells and accounts for successful fertilization in T. thermophila's multiple mating type system.
RESUMO
The multisubunit protein complex cohesin is required to establish cohesion between sister chromatids during S phase and to maintain it during G2 and M phases. Cohesin is essential for mitosis, and even partial defects cause very high rates of chromosome loss. In budding yeast, cohesin associates with specific sites which are distributed along the entire length of a chromosome but are more dense in the vicinity of the centromere. Real-time imaging of individual centromeres tagged with green fluorescent protein suggests that cohesin bound to centromeres is important for bipolar attachment to microtubules. This cohesin is, however, incapable of resisting the consequent force, which leads to sister centromere splitting and chromosome stretching. Meanwhile, cohesin bound to sequences flanking the centromeres prevents sister chromatids from completely unzipping and is required to pull back together sister centromeres that have already split. Cohesin therefore has a central role in generating a dynamic tension between microtubules and sister chromatid cohesion at centromeres, which lasts until chromosome segregation is finally promoted by separin-dependent cleavage of the cohesin subunit Scc1p.
Assuntos
Centrômero/metabolismo , Segregação de Cromossomos , Cromossomos Fúngicos/metabolismo , Microtúbulos/metabolismo , Proteínas Nucleares/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/citologia , Anáfase , Artefatos , Proteínas Cdc20 , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Proteínas de Ciclo Celular/fisiologia , Proteínas Cromossômicas não Histona , Cromossomos Fúngicos/genética , Replicação do DNA , Proteínas Fúngicas , Deleção de Genes , Hibridização in Situ Fluorescente , Modelos Biológicos , Regiões Operadoras Genéticas/genética , Fosfoproteínas , Saccharomyces cerevisiae/genética , Fuso Acromático/metabolismo , Sequências de Repetição em Tandem/genética , Fatores de Tempo , CoesinasRESUMO
In situ hybridization was used to examine chromosome behavior at meiotic prophase in the rad50S, hop1, rad50, and spo11 mutants of Saccharomyces cerevisiae, which are defective in chromosome synapsis and meiotic recombination. Painting of chromosomes I and III revealed that chromosome condensation and pairing are reduced in these mutants. However, there is some residual pairing in meiosis, suggesting that homologue recognition is independent of synaptonemal complex formation and recombination. Association of homologues was observed in the rad50, rad50S, and spo11 mutants, which are defective in the formation or processing of meiotic double-strand breaks. This indicates that double-strand breaks are not an essential component of the meiotic homology searching mechanism or that there exist additional or alternative mechanisms for locating homologues.
Assuntos
Cromossomos Fúngicos/ultraestrutura , Meiose/genética , Recombinação Genética/genética , Saccharomyces cerevisiae/genética , Complexo Sinaptonêmico/genética , Núcleo Celular/ultraestrutura , Hibridização in Situ Fluorescente , Microscopia Eletrônica , Mutação , Prófase/genética , Saccharomyces cerevisiae/ultraestruturaRESUMO
Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursors) of other organisms. The number of linear elements in haploid, diploid, and tetraploid strains is always higher than the chromosome number, implying that they are not formed continuously along the chromosomes. Time course experiments reveal that the elements appear after DNA replication and form networks and bundles. Later they separate and approximately 24 individual elements with a total length of 34 microns are observed before degradation and meiotic divisions. Parallel staining of DNA reveals changes in nuclear shape during meiotic prophase. Strains with a mei4 mutation are blocked at a late prophase stage. In serial sections we additionally observed a constant arrangement of the spindle pole body, the nucleolus, and the presumptive centromere cluster. Thus, S. pombe manages to recombine and segregate its chromosomes without SC. This might correlate with the absence of crossover interference. We propose a mechanism for chromosome pairing with initial recognition of the homologs at the centromeres and suggest functions of the linear elements in preparation of the chromosomes for meiosis I disjunction. With the spreading technique combined genetic, molecular, and cytological approaches become feasible in S. pombe. This provides an opportunity to study essential meiotic functions in the absence of SCs which may help to clarify the significance of the SC and its components for meiotic chromosome structure and function.
Assuntos
Núcleo Celular/ultraestrutura , Meiose , Schizosaccharomyces/citologia , Troca Genética , Técnicas Citológicas , Modelos Biológicos , Prófase , Schizosaccharomyces/ultraestrutura , Complexo SinaptonêmicoRESUMO
Chromosome arrangement in spread nuclei of the budding yeast, Saccharomyces cerevisiae was studied by fluorescence in situ hybridization with probes to centromeres and telomeric chromosome regions. We found that during interphase centromeres are tightly clustered in a peripheral region of the nucleus, whereas telomeres tend to occupy the area outside the centromeric domain. In vigorously growing cultures, centromere clustering occurred in approximately 90% of cells and it appeared to be maintained throughout interphase. It was reduced when cells were kept under stationary conditions for an extended period. In meiosis, centromere clusters disintegrated before the emergence of the earliest precursors of the synaptonemal complex. Evidence for the contribution of centromere clustering to other aspects of suprachromosomal nuclear order, in particular the vegetative association of homologous chromosomes, is provided, and a possible supporting role in meiotic homology searching is discussed.
Assuntos
Ciclo Celular/fisiologia , Núcleo Celular/ultraestrutura , Centrômero/ultraestrutura , Cromossomos Fúngicos/ultraestrutura , Saccharomyces cerevisiae/citologia , Anáfase , Núcleo Celular/fisiologia , Polaridade Celular , Centrômero/fisiologia , Cromossomos Fúngicos/fisiologia , Sondas de DNA , Hibridização in Situ Fluorescente , Meiose , Mitose , Prófase , Saccharomyces cerevisiae/ultraestrutura , Telômero/fisiologia , Telômero/ultraestruturaRESUMO
In yeast, HOP1 and RED1 are required during meiosis for proper chromosome segregation and the consequent formation of viable spores. Mutations in either HOP1 or RED1 create unique as well as overlapping phenotypes, indicating that the two proteins act alone as well as in concert with each other. To understand which meiotic processes specifically require Red1p-Hop1p hetero-oligomers, a novel genetic screen was used to identify a single-point mutation of RED1, red1-K348E, that separates Hop1p binding from Red1p homo-oligomerization. The Red1-K348E protein is stable, phosphorylated in a manner equivalent to Red1p, and undergoes efficient homo-oligomerization; however, its ability to interact with Hop1p both by two-hybrid and coimmunoprecipitation assays is greatly reduced. Overexpression of HOP1 specifically suppresses red1-K348E, supporting the idea that the only defect in the protein is a reduced affinity for Hop1p. red1-K348E mutants exhibit reduced levels of crossing over and spore viability and fail to undergo chromosome synapsis, thereby implicating a role for Red1p-Hop1p hetero-oligomers in these processes. Furthermore, red1-K348E suppresses the sae2/com1 defects in meiotic progression and sporulation, indicating a previously unknown role for HOP1 in the meiotic recombination checkpoint.
Assuntos
Segregação de Cromossomos/fisiologia , Cromossomos Fúngicos/fisiologia , Proteínas de Ligação a DNA/metabolismo , Proteínas Fúngicas/metabolismo , Meiose/fisiologia , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/fisiologia , Transdução de Sinais/fisiologia , Alelos , Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sítios de Ligação , Segregação de Cromossomos/genética , Troca Genética , Endonucleases , Proteínas Fúngicas/genética , Deleção de Genes , Expressão Gênica , Mutagênese , Oligopeptídeos/genética , Oligopeptídeos/metabolismo , Fenótipo , Recombinação Genética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo , Esporos Fúngicos/fisiologiaRESUMO
One of the consequences of hereditary peroxisomal dysfunction in the cerebro-hepato-renal (Zellweger) syndrome (CHRS) is a dramatic decrease in the biosynthesis and cellular content of ether lipids. In the present study effects of reduced cellular plasmalogen levels on membrane-membrane interactions were investigated. Cultured CHRS fibroblasts were incubated with unilamellar phospholipid vesicles consisting of 1-O-alkenyl-2-acyl- or 1,2-diacyl-sn-glycerophosphocholines and ethanolamines, carrying either the trans-parinaroyl or the 1,6-diphenyl-1,3,5-hexatriene propionyl group in position 2. Transfer of the fluorogenic phospholipids from vesicles to cells was followed by measuring the concomitant increase in fluorescence intensity. Transfer of phospholipids from cells to vesicles was monitored by incubating cells, prelabeled with [3H]oleic acid, in the presence of phospholipid vesicles. Fibroblasts from healthy donors or CHRS fibroblasts supplemented with the plasmalogen precursor 1-O-hexadecylglycerol served as controls. Plasmalogen-deficient cells exhibited a significantly increased tendency to take up exogenous choline or ethanolamine plasmalogens. Cellular plasmalogens were transferred from control cells to vesicles at a higher rate if the acceptor vesicles consisted of plasmalogens as compared to diacylglycerophosphocholine. Thus, it appears as if mechanisms existed which preserve cellular plasmalogen levels during interaction with exogenous phospholipid pools. Preliminary experimental evidence suggests that the observed exchange of phospholipids between cultured fibroblasts and vesicles occurs by a protein-catalyzed process.
Assuntos
Proteínas de Transporte/metabolismo , Fibroblastos/metabolismo , Fluorescência , Fosfolipídeos/metabolismo , Plasmalogênios/metabolismo , Transporte Biológico , Membrana Celular/metabolismo , Células Cultivadas , Humanos , Fosfolipídeos/químicaRESUMO
The influence of plasmalogen deficiency on membrane lipid mobility was determined by measuring fluorescence anisotropy of trimethylammoniumdiphenylhexatriene (TMA-DPH) and diphenylhexatrienylpropanoylhydrazylstachyose (glyco-DPH) inserted in the plasma membranes of human skin fibroblasts deficient in plasmalogens. The cells used were from patients affected with cerebrohepatorenal (Zellweger) syndrome (CHRS) or rhizomelic chondrodysplasia punctata. Their plasmalogen content (0-5% of total phospholipid) is significantly reduced compared with that of control cells from healthy donors (13-15% of total phospholipid) or of CHRS fibroblasts supplemented with the plasmalogen precursor, hexadecylglycerol. Plasmalogen-deficient cells consistently showed lower fluorescence anisotropies of membrane-bound DPH fluorophores corresponding to higher membrane lipid mobilities as compared to controls. However, very similar lipid mobilities were found for sonicated aqueous dispersions of phospholipids extracted either from CHRS or control cells. Therefore, the differences observed with living cells are not due to differences in the overall physical properties of the membrane lipid constituents. Other phenomena such as lipid asymmetry and/or plasmalogen-protein interactions may be responsible for the effects observed in the biomembranes.
Assuntos
Condrodisplasia Punctata/fisiopatologia , Fibroblastos/fisiologia , Fluidez de Membrana , Plasmalogênios/fisiologia , Síndrome de Zellweger/fisiopatologia , Membrana Celular/fisiologia , Difenilexatrieno/análogos & derivados , Polarização de Fluorescência , Corantes Fluorescentes , Humanos , Lipídeos de Membrana/fisiologia , OligopeptídeosRESUMO
Meiotic chromosome pairing in isogenic triploid and tetraploid strains of yeast and the consequences of polyploidy on meiotic chromosome segregation are studied. Synaptonemal complex formation at pachytene was found to be different in the triploid and in the tetraploid. In the triploid, triple-synapsis, that is, the connection of three homologues at a given site, is common. It can even extend all the way along the chromosomes. In the tetraploid, homologous chromosomes mostly come in pairs of synapsed bivalents. Multiple synapsis, that is, synapsis of more than two homologues in one and the same region, was virtually absent in the tetraploid. About five quadrivalents per cell occurred due to the switching of pairing partners. From the frequency of pairing partner switches it can be deduced that in most chromosomes synapsis is initiated primarily at one end, occasionally at both ends and rarely at an additional intercalary position. In contrast to a considerably reduced spore viability (approximately 40%) in the triploid, spore viability is only mildly affected in the tetraploid. The good spore viability is presumably due to the low frequency of quadrivalents and to the highly regular 2:2 segregation of the few quadrivalents that do occur. Occasionally, however, quadrivalents appear to be subject to 3:1 nondisjunction that leads to spore death in the second generation.
Assuntos
Cromossomos Fúngicos , Meiose/genética , Ploidias , Saccharomyces cerevisiae/genética , Cromossomos Fúngicos/ultraestrutura , Microscopia Eletrônica , Saccharomyces cerevisiae/fisiologia , Esporos FúngicosRESUMO
Chromosomes of altered size were found in the meiotic products of a haploid Saccharomyces cerevisiae strain by pulsed field gel electrophoretic separation of whole chromosomes. About 7% of haploid meioses produced chromosomes that differed by > or = 10 kb from their wild-type counterparts. Chromosomes most often became enlarged or shortened due to recombination events between sister chromatids at nonallelic sequences. By this mechanism chromosome III acquired tandem arrays of up to eight extra copies of the approximately kb MAT-HMR segment during repeated rounds of haploid meioses. Enlarged chromosomes III were unstable and changed their size during meiosis more often than remaining unchanged. Altered chromosomes appeared also as the products of intrachromatid recombination and of reciprocal translocations caused by ectopic recombination between nonhomologous chromosomes. In diploid meiosis, chromosomes of altered size occurred at least 10 times less frequently, whereas in mitotic cultures cells with altered karyotypes were virtually absent. The results show that various forms of ectopic recombination are promoted by the absence of allelic homologies.
Assuntos
Meiose/genética , Recombinação Genética , Saccharomyces cerevisiae/genética , Alelos , Cromátides , Cromossomos Fúngicos , Proteínas Fúngicas/genética , Haploidia , Cariotipagem , Sequências Repetitivas de Ácido Nucleico , Saccharomyces cerevisiae/citologia , Troca de Cromátide IrmãRESUMO
The MMS4 gene of Saccharomyces cerevisiae was originally identified due to its sensitivity to MMS in vegetative cells. Subsequent studies have confirmed a role for MMS4 in DNA metabolism of vegetative cells. In addition, mms4 diploids were observed to sporulate poorly. This work demonstrates that the mms4 sporulation defect is due to triggering of the meiotic recombination checkpoint. Genetic, physical, and cytological analyses suggest that MMS4 functions after the single end invasion step of meiotic recombination. In spo13 diploids, red1, but not mek1, is epistatic to mms4 for sporulation and spore viability, suggesting that MMS4 may be required only when homologs are capable of undergoing synapsis. MMS4 and MUS81 are in the same epistasis group for spore viability, consistent with biochemical data that show that the two proteins function in a complex. In contrast, MMS4 functions independently of MSH5 in the production of viable spores. We propose that MMS4 is required for the processing of specific recombination intermediates during meiosis.
Assuntos
Endonucleases , Meiose , Recombinação Genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Transativadores/fisiologia , Alelos , Proteínas de Ligação a DNA/genética , Diploide , Endonucleases Flap , Proteínas Fúngicas/genética , Genótipo , Modelos Genéticos , Mutação , Plasmídeos/metabolismo , Prófase , Fatores de Tempo , Transativadores/genética , Técnicas do Sistema de Duplo-HíbridoRESUMO
The Saccharomyces cerevisiae MUM2 gene is essential for meiotic, but not mitotic, DNA replication and thus sporulation. Genetic interactions between MUM2 and a component of the origin recognition complex and polymerase alpha-primase suggest that MUM2 influences the function of the DNA replication machinery. Early meiotic gene expression is induced to a much greater extent in mum2 cells than in meiotic cells treated with the DNA synthesis inhibitor hydroxyurea. This result indicates that the mum2 meiotic arrest is downstream of the arrest induced by hydroxyurea and suggests that DNA synthesis is initiated in the mutant. Genetic analyses indicate that the recombination that occurs in mum2 mutants is dependent on the normal recombination machinery and on synaptonemal complex components and therefore is not a consequence of lesions created by incompletely replicated DNA. Both meiotic ectopic and allelic recombination are similarly reduced in the mum2 mutant, and the levels are consistent with the levels of meiosis-specific DSBs that are generated. Cytological analyses of mum2 mutants show that chromosome pairing and synapsis occur, although at reduced levels compared to wild type. Given the near-wild-type levels of meiotic gene expression, pairing, and synapsis, we suggest that the reduction in DNA replication is directly responsible for the reduced level of DSBs and meiotic recombination.
Assuntos
Proteínas de Ciclo Celular , Dano ao DNA , Replicação do DNA , Proteínas Fúngicas/genética , Meiose/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Alelos , Southern Blotting , Citometria de Fluxo , Proteínas Fúngicas/fisiologia , Hibridização in Situ Fluorescente , Mutação , Plasmídeos/metabolismo , Estrutura Terciária de Proteína , RNA/metabolismo , Recombinação Genética , Temperatura , Fatores de TempoRESUMO
Mutation of the MER2 gene of Saccharomyces cerevisiae confers meiotic lethality. To gain insight into the function of the Mer2 protein, we have carried out a detailed characterization of the mer2 null mutant. Genetic analysis indicates that mer2 completely eliminates meiotic interchromosomal gene conversion and crossing over. In addition, mer2 abolishes intrachromosomal meiotic recombination, both in the ribosomal DNA array and in an artificial duplication. The results of a physical assay demonstrate that the mer2 mutation prevents the formation of meiosis-specific, double-strand breaks, indicating that the Mer2 protein acts at or before the initiation of meiotic recombination. Electron microscopic analysis reveals that the mer2 mutant makes axial elements, which are precursors to the synaptonemal complex, but homologous chromosomes fail to synapse. Fluorescence in situ hybridization of chromosome-specific DNA probes to spread meiotic chromosomes demonstrates that homolog alignment is also significantly reduced in the mer2 mutant. Although the MER2 gene is transcribed during vegetative growth, deletion or overexpression of the MER2 gene has no apparent effect on mitotic recombination or DNA damage repair. We suggest that the primary defect in the mer2 mutant is in the initiation of meiotic genetic exchange.
Assuntos
Cromossomos Fúngicos/fisiologia , Proteínas Fúngicas/genética , Meiose/genética , Recombinação Genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Genes Fúngicos , MutaçãoRESUMO
The fluorescence decay of alkenylparinaroyl- and palmitoylparinaroyl glycerophosphocholines in vesicles of the unlabeled alkenyloleoyl and palmitoyloleoyl analogs was determined by multifrequency phase and modulation fluorometry. The measured phase angles and demodulations could be equally well fitted to a biexponential decay, as well as unimodal or bimodal continuous lifetime distributions. The latter model was applied to study the influence of cholesterol on parinaroyl phospholipid fluorescence in vesicles. The long-living component of a bimodal lifetime distribution was sensitive toward the presence of the sterol. Upon increasing cholesterol concentrations, its lifetime center increased and its distribution widths decreased. Lifetime distribution widths in vesicles of alkenyloleoyl- or palmitoyloleoyl-glycerophosphocholine (choline plasmalogen and phosphatidylcholine, respectively) were reduced by the sterol to the same extent. We interprete the sterol-induced lifetime distribution narrowing as an effect due to an increase of membrane homogeneity in cholesterol-phospholipid membranes.
Assuntos
Colesterol/análise , Bicamadas Lipídicas/química , Lipossomos/química , Fosfatidilcolinas/química , Corantes Fluorescentes , Plasmalogênios , Espectrometria de FluorescênciaRESUMO
The potential of ionic liquids as solvents for headspace gas chromatography was investigated. Three compounds with boiling points above 200 degrees C were selected to demonstrate the feasibility of the concept described. 2-Ethylhexanoic acid, formamide, and tri-n-butylamine as examples of acidic, neutral, and basic analytes were dissolved in acidic 1-n-butyl-3-methylimidazolium hydrogen sulfate (1), neutral 1-n-butyl-2,3-dimethylimidazolium dicyanamide (2), and 2 containing 1,8-diazabicyclo[5.4.0]undec-7-ene to adjust basic conditions. All analytes could be determined with limits of detection and limits of quantification in the low-ppm concentration range.
Assuntos
Cromatografia Gasosa/métodos , Imidazóis/química , Íons , Solventes/química , Ampicilina/isolamento & purificação , Compostos Bicíclicos Heterocíclicos com Pontes/química , Butilaminas/química , Caproatos/química , Ácido Clavulânico/isolamento & purificação , Formamidas/química , Penicilina G Benzatina/isolamento & purificação , PressãoRESUMO
In Allium ursinum meiotic pairing of homologues is always incomplete; a proximal region on each bivalent remains regularly unsynapsed even in late pachytene. The spatial correlation of the unsynapsed region with the kinetochore suggests that the kinetochore itself exerts an inhibitory effect on synapsis in its vicinity. This can be interpreted as the cytological basis of the 'centromere effect' on recombination in this species. Moreover, the high incidence of a pericentric inversion loop in a heterozygous chromosome pair shows that proximal pairing initiation is possible and that its failure cannot be responsible for pericentric asynapsis. The formation of the inversion loop is complicated by the need for two independent pairing initiation sites because synapsis cannot proceed across the pericentric region. It is proposed that the meiotic bouquet polarization helps in establishing the presynaptic alignment of the homologous sites within the inverted regions and hence to achieve a high rate of inversion loop formation. Thickenings of the axial/lateral elements are not distributed equally along the synaptonemal complex. They are underrepresented in unpaired axes but strikingly abundant at the borders with synapsed regions, suggesting their origin in the pairing forks during the process of synapsis. They are virtually always present at nucleolus-organizing regions and often they appear at corresponding sites on opposite lateral elements. Besides the thickenings several other kinds of axial deformities are present in unpaired axes.
Assuntos
Allium/genética , Cromossomos/ultraestrutura , Meiose , Complexo Sinaptonêmico , Allium/ultraestrutura , Inversão CromossômicaRESUMO
This article reviews current views on the mechanisms of meiotic homology searching and recombination. It discusses the relationship between molecular events at meiotic prophase and concomitant cytological processes. The role of the synaptonemal complex and other meiosis-specific structures is discussed. Whereas the relationship of crossovers, late recombination nodules, and chiasmata is well established, there is still some controversy about the temporal and causal relationships between double strand breaks, homologue recognition, heteroduplexes, early nodules and presynaptic alignment.
Assuntos
Cromossomos/ultraestrutura , Meiose , Recombinação Genética , Homologia de Sequência do Ácido Nucleico , Complexo Sinaptonêmico/fisiologiaRESUMO
Metaphase chromosome bands were induced in Allium flavum (Liliaceae) by protecting the chromosomal DNA with DNA-binding compounds of different base specificities against DNase digestion. The bands obtained represented different subsets of C-band heterochromatin.
Assuntos
Bandeamento Cromossômico , DNA/metabolismo , Desoxirribonucleases/antagonistas & inibidores , Corantes Fluorescentes , Plantas/ultraestrutura , Cromomicina A3 , Dactinomicina , Distamicinas , CariotipagemRESUMO
Interference of colchicine with meiotic chromosome pairing in the wild garlic, Allium ursinum, was studied using a whole-mount spreading technique for synaptonemal complexes. Colchicine was found to cause (i) pairing suppression (arrest of leptotene) and (ii) deficient pairing initiation at zygotene in connection with morphologically anomalous, malfunctioning pairing initiation sites. Both of these phenomena could be responsible for the reduction of chiasma frequency by colchicine previously reported in the literature.