Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 96
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
PLoS Comput Biol ; 20(4): e1011855, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38578817

RESUMO

The collective migration of keratinocytes during wound healing requires both the generation and transmission of mechanical forces for individual cellular locomotion and the coordination of movement across cells. Leader cells along the wound edge transmit mechanical and biochemical cues to ensuing follower cells, ensuring their coordinated direction of migration across multiple cells. Despite the observed importance of mechanical cues in leader cell formation and in controlling coordinated directionality of cell migration, the underlying biophysical mechanisms remain elusive. The mechanically-activated ion channel PIEZO1 was recently identified to play an inhibitory role during the reepithelialization of wounds. Here, through an integrative experimental and mathematical modeling approach, we elucidate PIEZO1's contributions to collective migration. Time-lapse microscopy reveals that PIEZO1 activity inhibits leader cell formation at the wound edge. To probe the relationship between PIEZO1 activity, leader cell formation and inhibition of reepithelialization, we developed an integrative 2D continuum model of wound closure that links observations at the single cell and collective cell migration scales. Through numerical simulations and subsequent experimental validation, we found that coordinated directionality plays a key role during wound closure and is inhibited by upregulated PIEZO1 activity. We propose that PIEZO1-mediated retraction suppresses leader cell formation which inhibits coordinated directionality between cells during collective migration.


Assuntos
Canais Iônicos , Queratinócitos , Movimento Celular/fisiologia
2.
PLoS Comput Biol ; 18(5): e1010039, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35522694

RESUMO

Feedback mechanisms within cell lineages are thought to be important for maintaining tissue homeostasis. Mathematical models that assume well-mixed cell populations, together with experimental data, have suggested that negative feedback from differentiated cells on the stem cell self-renewal probability can maintain a stable equilibrium and hence homeostasis. Cell lineage dynamics, however, are characterized by spatial structure, which can lead to different properties. Here, we investigate these dynamics using spatially explicit computational models, including cell division, differentiation, death, and migration / diffusion processes. According to these models, the negative feedback loop on stem cell self-renewal fails to maintain homeostasis, both under the assumption of strong spatial restrictions and fast migration / diffusion. Although homeostasis cannot be maintained, this feedback can regulate cell density and promote the formation of spatial structures in the model. Tissue homeostasis, however, can be achieved if spatially restricted negative feedback on self-renewal is combined with an experimentally documented spatial feedforward loop, in which stem cells regulate the fate of transit amplifying cells. This indicates that the dynamics of feedback regulation in tissue cell lineages are more complex than previously thought, and that combinations of spatially explicit control mechanisms are likely instrumental.


Assuntos
Modelos Biológicos , Células-Tronco , Diferenciação Celular/fisiologia , Linhagem da Célula , Retroalimentação
3.
PLoS Comput Biol ; 18(6): e1010288, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35737645

RESUMO

[This corrects the article DOI: 10.1371/journal.pcbi.1009701.].

4.
J Math Biol ; 86(6): 97, 2023 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-37219647

RESUMO

We present a phase field model for vesicle growth or shrinkage induced by an osmotic pressure due to a chemical potential gradient. The model consists of an Allen-Cahn equation describing the evolution of the phase field parameter that describes the shape of the vesicle and a Cahn-Hilliard-type equation describing the evolution of the ionic fluid. We establish conditions for vesicle growth or shrinkage via a common tangent construction using free energy curves. During the membrane deformation, the model ensures total mass conservation of the ionic fluid, and we weakly enforce a surface area constraint of the vesicle. We develop a stable numerical scheme and an efficient nonlinear multigrid solver to evolve the phase and concentration fields, and we use this to evolve the fields to near equilibrium for 2D vesicles. Convergence tests confirm an [Formula: see text] accuracy for our scheme and near-optimal convergence for our multigrid solver. Numerical results reveal that the diffuse interface model captures the main features of cell shape dynamics: for a growing vesicle, there exist circle-like equilibrium shapes if the concentration difference across the membrane and the initial osmotic pressure are large enough; while for a shrinking vesicle, there exists a rich collection of finger-like equilibrium morphologies.


Assuntos
Forma Celular
5.
PLoS Comput Biol ; 17(12): e1009701, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34932555

RESUMO

Experiments on tumor spheroids have shown that compressive stress from their environment can reversibly decrease tumor expansion rates and final sizes. Stress release experiments show that nonuniform anisotropic elastic stresses can be distributed throughout. The elastic stresses are maintained by structural proteins and adhesive molecules, and can be actively relaxed by a variety of biophysical processes. In this paper, we present a new continuum model to investigate how the growth-induced elastic stresses and active stress relaxation, in conjunction with cell size control feedback machinery, regulate the cell density and stress distributions within growing tumors as well as the tumor sizes in the presence of external physical confinement and gradients of growth-promoting chemical fields. We introduce an adaptive reference map that relates the current position with the reference position but adapts to the current position in the Eulerian frame (lab coordinates) via relaxation. This type of stress relaxation is similar to but simpler than the classical Maxwell model of viscoelasticity in its formulation. By fitting the model to experimental data from two independent studies of tumor spheroid growth and their cell density distributions, treating the tumors as incompressible, neo-Hookean elastic materials, we find that the rates of stress relaxation of tumor tissues can be comparable to volumetric growth rates. Our study provides insight on how the biophysical properties of the tumor and host microenvironment, mechanical feedback control and diffusion-limited differential growth act in concert to regulate spatial patterns of stress and growth. When the tumor is stiffer than the host, our model predicts tumors are more able to change their size and mechanical state autonomously, which may help to explain why increased tumor stiffness is an established hallmark of malignant tumors.


Assuntos
Fenômenos Biomecânicos/fisiologia , Proliferação de Células/fisiologia , Neoplasias , Anisotropia , Linhagem Celular Tumoral , Biologia Computacional , Humanos , Neoplasias/patologia , Neoplasias/fisiopatologia , Esferoides Celulares/citologia , Esferoides Celulares/fisiologia , Estresse Mecânico , Células Tumorais Cultivadas
6.
J Math Biol ; 85(1): 5, 2022 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-35796898

RESUMO

We study a classic Darcy's law model for tumor cell motion with inhomogeneous and isotropic conductivity. The tumor cells are assumed to be a constant density fluid flowing through porous extracellular matrix (ECM). The ECM is assumed to be rigid and motionless with constant porosity. One and two dimensional simulations show that the tumor mass grows from high to low conductivity regions when the tumor morphology is steady. In the one-dimensional case, we proved that when the tumor size is steady, the tumor grows towards lower conductivity regions. We conclude that this phenomenon is produced by the coupling of a special inward flow pattern in the steady tumor and Darcy's law which gives faster flow speed in higher conductivity regions.


Assuntos
Neoplasias , Condutividade Elétrica , Matriz Extracelular , Humanos , Porosidade
7.
J Theor Biol ; 509: 110499, 2021 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-33130064

RESUMO

While resistance mutations are often implicated in the failure of cancer therapy, lack of response also occurs without such mutants. In bladder cancer mouse xenografts, repeated chemotherapy cycles have resulted in cancer stem cell (CSC) enrichment, and consequent loss of therapy response due to the reduced susceptibility of CSCs to drugs. A particular feedback loop present in the xenografts has been shown to promote CSC enrichment in this system. Yet, many other regulatory loops might also be operational and might promote CSC enrichment. Their identification is central to improving therapy response. Here, we perform a comprehensive mathematical analysis to define what types of regulatory feedback loops can and cannot contribute to CSC enrichment, providing guidance to the experimental identification of feedback molecules. We derive a formula that reveals whether or not the cell population experiences CSC enrichment over time, based on the properties of the feedback. We find that negative feedback on the CSC division rate or positive feedback on differentiated cell death rate can lead to CSC enrichment. Further, the feedback mediators that achieve CSC enrichment can be secreted by either CSCs or by more differentiated cells. The extent of enrichment is determined by the CSC death rate, the CSC self-renewal probability, and by feedback strength. Defining these general characteristics of feedback loops can guide the experimental screening for and identification of feedback mediators that can promote CSC enrichment in bladder cancer and potentially other tumors. This can help understand and overcome the phenomenon of CSC-based therapy resistance.


Assuntos
Resistencia a Medicamentos Antineoplásicos , Neoplasias , Animais , Diferenciação Celular , Linhagem Celular Tumoral , Retroalimentação , Camundongos , Células-Tronco Neoplásicas
8.
PLoS Comput Biol ; 15(4): e1006352, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31022168

RESUMO

In many biological settings, two or more cells come into physical contact to form a cell-cell interface. In some cases, the cell-cell contact must be transient, forming on timescales of seconds. One example is offered by the T cell, an immune cell which must attach to the surface of other cells in order to decipher information about disease. The aspect ratio of these interfaces (tens of nanometers thick and tens of micrometers in diameter) puts them into the thin-layer limit, or "lubrication limit", of fluid dynamics. A key question is how the receptors and ligands on opposing cells come into contact. What are the relative roles of thermal undulations of the plasma membrane and deterministic forces from active filopodia? We use a computational fluid dynamics algorithm capable of simulating 10-nanometer-scale fluid-structure interactions with thermal fluctuations up to seconds- and microns-scales. We use this to simulate two opposing membranes, variously including thermal fluctuations, active forces, and membrane permeability. In some regimes dominated by thermal fluctuations, proximity is a rare event, which we capture by computing mean first-passage times using a Weighted Ensemble rare-event computational method. Our results demonstrate a parameter regime in which the time it takes for an active force to drive local contact actually increases if the cells are being held closer together (e.g., by nonspecific adhesion), a phenomenon we attribute to the thin-layer effect. This leads to an optimal initial cell-cell separation for fastest receptor-ligand binding, which could have relevance for the role of cellular protrusions like microvilli. We reproduce a previous experimental observation that fluctuation spatial scales are largely unaffected, but timescales are dramatically slowed, by the thin-layer effect. We also find that membrane permeability would need to be above physiological levels to abrogate the thin-layer effect.


Assuntos
Permeabilidade da Membrana Celular/fisiologia , Membrana Celular/fisiologia , Extensões da Superfície Celular/fisiologia , Hidrodinâmica , Modelos Biológicos , Algoritmos , Adesão Celular/fisiologia , Biologia Computacional/métodos
9.
Bull Math Biol ; 82(3): 39, 2020 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-32166456

RESUMO

In this paper, we develop a sharp interface tumor growth model to study the effect of the tumor microenvironment using a complex far-field geometry that mimics a heterogeneous distribution of vasculature. Together with different nutrient uptake rates inside and outside the tumor, this introduces variability in spatial diffusion gradients. Linear stability analysis suggests that the uptake rate in the tumor microenvironment, together with chemotaxis, may induce unstable growth, especially when the nutrient gradients are large. We investigate the fully nonlinear dynamics using a spectrally accurate boundary integral method. Our nonlinear simulations reveal that vascular heterogeneity plays an important role in the development of morphological instabilities that range from fingering and chain-like morphologies to compact, plate-like shapes in two dimensions.


Assuntos
Quimiotaxia/fisiologia , Modelos Biológicos , Neoplasias/patologia , Neoplasias/fisiopatologia , Apoptose , Transporte Biológico Ativo , Proliferação de Células , Simulação por Computador , Humanos , Modelos Lineares , Conceitos Matemáticos , Invasividade Neoplásica , Neoplasias/irrigação sanguínea , Dinâmica não Linear , Nutrientes/metabolismo , Microambiente Tumoral/fisiologia
10.
Math Models Methods Appl Sci ; 30(8): 1591-1651, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35309741

RESUMO

This paper is devoted to the multidisciplinary modelling of a pandemic initiated by an aggressive virus, specifically the so-called SARS-CoV-2 Severe Acute Respiratory Syndrome, corona virus n.2. The study is developed within a multiscale framework accounting for the interaction of different spatial scales, from the small scale of the virus itself and cells, to the large scale of individuals and further up to the collective behaviour of populations. An interdisciplinary vision is developed thanks to the contributions of epidemiologists, immunologists and economists as well as those of mathematical modellers. The first part of the contents is devoted to understanding the complex features of the system and to the design of a modelling rationale. The modelling approach is treated in the second part of the paper by showing both how the virus propagates into infected individuals, successfully and not successfully recovered, and also the spatial patterns, which are subsequently studied by kinetic and lattice models. The third part reports the contribution of research in the fields of virology, epidemiology, immune competition, and economy focussed also on social behaviours. Finally, a critical analysis is proposed looking ahead to research perspectives.

11.
J Theor Biol ; 463: 138-154, 2019 02 21.
Artigo em Inglês | MEDLINE | ID: mdl-30528340

RESUMO

In this paper, we apply the diffuse domain framework developed in Chen and Lowengrub (Tumor growth in complex, evolving microenvironmental geometries: A diffuse domain approach, J. Theor. Biol. 361 (2014) 14-30) to study the effects of a deformable basement membrane (BM) on the growth of a tumor in a confined, ductal geometry, such as ductal carcinoma in situ (DCIS). We use a continuum model of tumor microcalcification and investigate the tumor extent beyond the microcalcification. In order to solve the governing equations efficiently, we develop a stable nonlinear multigrid finite difference method. Two dimensional simulations are performed where the adhesion between tumor cells and the basement membrane is varied. Additional simulations considering the variation of duct radius and membrane stiffness are also conducted. The results demonstrate that enhanced membrane deformability promotes tumor growth and tumor calcification. When the duct radius is small, the cell-BM adhesion is weak or when the membrane is slightly deformed, the mammographic and pathologic tumor extents are linearly correlated, as predicted by Macklin et al. (J. Theor. Biol. 301 (2012) 122-140) using an agent-based model that does not account for the deformability of the basement membrane and the active forces that the membrane imparts on the tumor cells. Interestingly, we predict that when the duct radius is large, there is strong cell-BM adhesion or the membrane is highly deformed, the extents of the mammographic and pathologic tumors are instead quadratically correlated. The simulations can help surgeons to measure DCIS surgical margins while removing less non-cancerous tissue, and can improve targeting of intra- and post-operative radiotherapy.


Assuntos
Calcinose , Modelos Biológicos , Neoplasias/patologia , Membrana Basal/metabolismo , Membrana Basal/ultraestrutura , Carcinoma Ductal de Mama/patologia , Carcinoma Ductal de Mama/ultraestrutura , Adesão Celular , Simulação por Computador , Humanos
12.
Mol Syst Biol ; 13(2): 912, 2017 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-28183841

RESUMO

Cell-intrinsic metabolic reprogramming is a hallmark of cancer that provides anabolic support to cell proliferation. How reprogramming influences tumor heterogeneity or drug sensitivities is not well understood. Here, we report a self-organizing spatial pattern of glycolysis in xenograft colon tumors where pyruvate dehydrogenase kinase (PDK1), a negative regulator of oxidative phosphorylation, is highly active in clusters of cells arranged in a spotted array. To understand this pattern, we developed a reaction-diffusion model that incorporates Wnt signaling, a pathway known to upregulate PDK1 and Warburg metabolism. Partial interference with Wnt alters the size and intensity of the spotted pattern in tumors and in the model. The model predicts that Wnt inhibition should trigger an increase in proteins that enhance the range of Wnt ligand diffusion. Not only was this prediction validated in xenograft tumors but similar patterns also emerge in radiochemotherapy-treated colorectal cancer. The model also predicts that inhibitors that target glycolysis or Wnt signaling in combination should synergize and be more effective than each treatment individually. We validated this prediction in 3D colon tumor spheroids.


Assuntos
Neoplasias do Colo/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Regulação para Cima , Via de Sinalização Wnt , Animais , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Glicólise , Humanos , Camundongos , Modelos Teóricos , Transplante de Neoplasias , Piruvato Desidrogenase Quinase de Transferência de Acetil
13.
J Theor Biol ; 439: 86-99, 2018 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-29203124

RESUMO

The tumor microenvironment is an integral component in promoting tumor development. Cancer-associated fibroblasts (CAFs), which reside in the tumor stroma, produce Hepatocyte Growth Factor (HGF), an important trigger for invasive and metastatic tumor behavior. HGF contributes to a pro-tumorigenic environment by activating its cognate receptor, c-Met, on tumor cells. Tumor cells, in turn, secrete growth factors that upregulate HGF production in CAFs, thereby establishing a dynamic tumor-host signaling program. Using a spatiotemporal multispecies model of tumor growth, we investigate how the development and spread of a tumor is impacted by the initiation of a dynamic interaction between tumor-derived growth factors and CAF-derived HGF. We show that establishment of such an interaction results in increased tumor growth and morphological instability, the latter due in part to increased cell species heterogeneity at the tumor-host boundary. Invasive behavior is further increased if the tumor lowers responsiveness to paracrine pro-differentiation signals, which is a hallmark of neoplastic development. By modeling anti-HGF and anti-c-Met therapy, we show how disruption of the HGF/c-Met axis can reduce tumor invasiveness and growth, thereby providing theoretical evidence that targeting tumor-microenvironment interactions is a promising avenue for therapeutic development.


Assuntos
Fator de Crescimento de Hepatócito/metabolismo , Modelos Biológicos , Comunicação Parácrina , Proteínas Proto-Oncogênicas c-met/metabolismo , Microambiente Tumoral , Animais , Fibroblastos Associados a Câncer/metabolismo , Proliferação de Células , Humanos , Invasividade Neoplásica , Análise Espaço-Temporal
14.
Soft Matter ; 14(10): 1833-1846, 2018 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-29451285

RESUMO

Preparation of thin films by dissolving polymers in a common solvent followed by evaporation of the solvent has become a routine processing procedure. However, modeling of thin film formation in an evaporating solvent has been challenging due to a need to simulate processes at multiple length and time scales. In this work, we present a methodology based on the principles of linear non-equilibrium thermodynamics, which allows systematic study of various effects such as the changes in the solvent properties due to phase transformation from liquid to vapor and polymer thermodynamics resulting from such solvent transformations. The methodology allows for the derivation of evaporative flux and boundary conditions near each surface for simulations of systems close to the equilibrium. We apply it to study thin film microstructural evolution in phase segregating polymer blends dissolved in a common volatile solvent and deposited on a planar substrate. Effects of the evaporation rates, interactions of the polymers with the underlying substrate and concentration dependent mobilities on the kinetics of thin film formation are studied.

15.
Bull Math Biol ; 80(5): 1404-1433, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-28681151

RESUMO

We develop a three-dimensional multispecies mathematical model to simulate the growth of colon cancer organoids containing stem, progenitor and terminally differentiated cells, as a model of early (prevascular) tumor growth. Stem cells (SCs) secrete short-range self-renewal promoters (e.g., Wnt) and their long-range inhibitors (e.g., Dkk) and proliferate slowly. Committed progenitor (CP) cells proliferate more rapidly and differentiate to produce post-mitotic terminally differentiated cells that release differentiation promoters, forming negative feedback loops on SC and CP self-renewal. We demonstrate that SCs play a central role in normal and cancer colon organoids. Spatial patterning of the SC self-renewal promoter gives rise to SC clusters, which mimic stem cell niches, around the organoid surface, and drive the development of invasive fingers. We also study the effects of externally applied signaling factors. Applying bone morphogenic proteins, which inhibit SC and CP self-renewal, reduces invasiveness and organoid size. Applying hepatocyte growth factor, which enhances SC self-renewal, produces larger sizes and enhances finger development at low concentrations but suppresses fingers at high concentrations. These results are consistent with recent experiments on colon organoids. Because many cancers are hierarchically organized and are subject to feedback regulation similar to that in normal tissues, our results suggest that in cancer, control of cancer stem cell self-renewal should influence the size and shape in similar ways, thereby opening the door to novel therapies.


Assuntos
Autorrenovação Celular , Neoplasias do Colo/patologia , Modelos Biológicos , Células-Tronco Neoplásicas/patologia , Animais , Morte Celular , Diferenciação Celular , Linhagem da Célula , Simulação por Computador , Retroalimentação Fisiológica , Humanos , Imageamento Tridimensional , Conceitos Matemáticos , Organoides/patologia , Análise Espaço-Temporal
16.
J Math Biol ; 77(3): 671-709, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29546457

RESUMO

We consider the nonlinear dynamics of an avascular tumor at the tissue scale using a two-fluid flow Stokes model, where the viscosity of the tumor and host microenvironment may be different. The viscosities reflect the combined properties of cell and extracellular matrix mixtures. We perform a linear morphological stability analysis of the tumors, and we investigate the role of nonlinearity using boundary-integral simulations in two dimensions. The tumor is non-necrotic, although cell death may occur through apoptosis. We demonstrate that tumor evolution is regulated by a reduced set of nondimensional parameters that characterize apoptosis, cell-cell/cell-extracellular matrix adhesion, vascularization and the ratio of tumor and host viscosities. A novel reformulation of the equations enables the use of standard boundary integral techniques to solve the equations numerically. Nonlinear simulation results are consistent with linear predictions for nearly circular tumors. As perturbations develop and grow, the linear and nonlinear results deviate and linear theory tends to underpredict the growth of perturbations. Simulations reveal two basic types of tumor shapes, depending on the viscosities of the tumor and microenvironment. When the tumor is more viscous than its environment, the tumors tend to develop invasive fingers and a branched-like structure. As the relative ratio of the tumor and host viscosities decreases, the tumors tend to grow with a more compact shape and develop complex invaginations of healthy regions that may become encapsulated in the tumor interior. Although our model utilizes a simplified description of the tumor and host biomechanics, our results are consistent with experiments in a variety of tumor types that suggest that there is a positive correlation between tumor stiffness and tumor aggressiveness.


Assuntos
Modelos Biológicos , Neoplasias/patologia , Apoptose , Adesão Celular , Simulação por Computador , Matriz Extracelular/patologia , Matriz Extracelular/fisiologia , Humanos , Modelos Lineares , Conceitos Matemáticos , Fluidez de Membrana , Invasividade Neoplásica/patologia , Invasividade Neoplásica/fisiopatologia , Neoplasias/irrigação sanguínea , Neoplasias/fisiopatologia , Neovascularização Patológica , Dinâmica não Linear , Esferoides Celulares/patologia , Esferoides Celulares/fisiologia , Células Tumorais Cultivadas , Microambiente Tumoral/fisiologia , Viscosidade
17.
PLoS Comput Biol ; 12(3): e1004814, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26989903

RESUMO

Feedback regulation of cell lineage progression plays an important role in tissue size homeostasis, but whether such feedback also plays an important role in tissue morphogenesis has yet to be explored. Here we use mathematical modeling to show that a particular feedback architecture in which both positive and negative diffusible signals act on stem and/or progenitor cells leads to the appearance of bistable or bi-modal growth behaviors, ultrasensitivity to external growth cues, local growth-driven budding, self-sustaining elongation, and the triggering of self-organization in the form of lamellar fingers. Such behaviors arise not through regulation of cell cycle speeds, but through the control of stem or progenitor self-renewal. Even though the spatial patterns that arise in this setting are the result of interactions between diffusible factors with antagonistic effects, morphogenesis is not the consequence of Turing-type instabilities.


Assuntos
Diferenciação Celular/fisiologia , Linhagem da Célula/fisiologia , Retroalimentação Fisiológica/fisiologia , Modelos Biológicos , Células-Tronco/citologia , Células-Tronco/fisiologia , Animais , Proteínas de Ciclo Celular/metabolismo , Simulação por Computador , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Humanos , Morfogênese/fisiologia , Transdução de Sinais/fisiologia
18.
Soft Matter ; 13(19): 3521-3531, 2017 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-28440378

RESUMO

We study the fully nonlinear, nonlocal dynamics of two-dimensional multicomponent vesicles in a shear flow with matched viscosity of the inner and outer fluids. Using a nonstiff, pseudo-spectral boundary integral method, we investigate dynamical patterns induced by inhomogeneous bending for a two phase system. Numerical results reveal that there exist novel phase-treading and tumbling mechanisms that cannot be observed for a homogeneous vesicle. In particular, unlike the well-known steady tank-treading dynamics characterized by a fixed inclination angle, here the phase-treading mechanism leads to unsteady periodic dynamics with an oscillatory inclination angle. When the average phase concentration is around 1/2, we observe tumbling dynamics even for very low shear rate, and the excess length required for tumbling is significantly smaller than the value for the single phase case. We summarize our results in phase diagrams in terms of the excess length, shear rate, and concentration of the soft phase. These findings go beyond the well known dynamical regimes of a homogeneous vesicle and highlight the level of complexity of vesicle dynamics in a fluid due to heterogeneous material properties.

19.
Biophys J ; 110(7): 1636-1647, 2016 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-27074688

RESUMO

Blebs are pressure-driven cell protrusions implicated in cellular functions such as cell division, apoptosis, and cell motility, including motility of protease-inhibited cancer cells. Because of their mechanical nature, blebs inform us about general cell-surface mechanics, including membrane dynamics, pressure propagation throughout the cytoplasm, and the architecture and dynamics of the actin cortex. Mathematical models including detailed fluid dynamics have previously been used to understand bleb expansion. Here, we develop mathematical models in two and three dimensions on longer timescales that recapitulate the full bleb life cycle, including both expansion and healing by cortex reformation, in terms of experimentally accessible biophysical parameters such as myosin contractility, osmotic pressure, and turnover of actin and ezrin. The model provides conditions under which blebbing occurs, and naturally gives rise to traveling blebs. The model predicts conditions under which blebs travel or remain stationary, as well as the bleb traveling velocity, a quantity that has remained elusive in previous models. As previous studies have used blebs as reporters of membrane tension and pressure dynamics within the cell, we have used our system to investigate various pressure equilibration models and dynamic, nonuniform membrane tension to account for the shape of a traveling bleb. We also find that traveling blebs tend to expand in all directions unless otherwise constrained. One possible constraint could be provided by spatial heterogeneity in, for example, adhesion density.


Assuntos
Extensões da Superfície Celular/metabolismo , Fenômenos Mecânicos , Modelos Biológicos , Fenômenos Biomecânicos , Cinética
20.
Soft Matter ; 12(26): 5663-75, 2016 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-27136977

RESUMO

We study the fully nonlinear, nonlocal dynamics of two-dimensional vesicles in a time-dependent, incompressible viscous flow at finite temperature. We focus on a transient instability that can be observed when the direction of applied flow is suddenly reversed, which induces compressive forces on the vesicle interface, and small-scale interface perturbations known as wrinkles develop. These wrinkles are driven by regions of negative elastic tension on the membrane. Using a stochastic immersed boundary method with a biophysically motivated choice of thermal fluctuations, we investigate the wrinkling dynamics numerically. Different from deterministic wrinkling dynamics, thermal fluctuations lead to symmetry-breaking wrinkling patterns by exciting higher order modes. This leads to more rapid and more realistic wrinkling dynamics. Our results are in excellent agreement with the experimental data by Kantsler et al. [Kantsler et al., Phys. Rev. Lett., 2007, 99, 17802]. We compare the nonlinear simulation results with perturbation theory, modified to account for thermal fluctuations. The strength of the applied flow strongly influences the most unstable wavelength characterizing the wrinkles, and there are significant differences between the results from perturbation theory and the fully nonlinear simulations, which suggests that the perturbation theory misses important nonlinear interactions. Strikingly, we find that thermal fluctuations actually have the ability to attenuate variability of the characteristic wavelength of wrinkling by exciting a wider range of modes than the deterministic case, which makes the evolution less constrained and enables the most unstable wavelength to emerge more readily. We further find that thermal noise helps prevent the vesicle from rotating if it is misaligned with the direction of the applied extensional flow.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA