RESUMO
PCR amplification indicated the minimum infection rate of Rickettsia spp. was 0.66% in Haemaphysalis longicornis ticks collected from Shandong Province, China. Phylogenetic analysis based on the rrs, gltA, ompA, and ompB genes indicated that the ticks carried R. japonica, Candidatus Rickettsia longicornii, and a novel Rickettsia species related to R. canadensis.
Assuntos
Rickettsia/classificação , Rickettsia/genética , Carrapatos/microbiologia , Animais , China/epidemiologia , DNA Bacteriano , Humanos , Filogenia , Reação em Cadeia da Polimerase , Vigilância em Saúde Pública , Doenças Transmitidas por Vetores/epidemiologia , Doenças Transmitidas por Vetores/microbiologiaRESUMO
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever in East Asia caused by SFTS virus (SFTSV), a newly discovered phlebovirus. The Haemaphysalis longicornis tick has been suspected to be the vector of SFTSV. To determine whether SFTSV can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of H. longicornis ticks and between ticks and mice. Using reverse transcription PCR, we also analyzed the prevalence of SFTSV infection among H. longicornis ticks collected from vegetation in Shandong Province, China. Our results showed a low prevalence of SFTSV among collected ticks (0.2%, 8/3,300 ticks), and we showed that ticks fed on SFTSV-infected mice could acquire the virus and transstadially and transovarially transmit it to other developmental stages of ticks. Furthermore, SFTSV-infected ticks could transmit the virus to mice during feeding. Our findings indicate ticks could serve as a vector and reservoir of SFTSV.
Assuntos
Vetores Aracnídeos/virologia , Infecções por Bunyaviridae/transmissão , Transmissão de Doença Infecciosa/veterinária , Phlebovirus/genética , Phlebovirus/patogenicidade , Carrapatos/virologia , Viroses/transmissão , Animais , Infecções por Bunyaviridae/virologia , China/epidemiologia , Camundongos , Carrapatos/microbiologia , Viroses/virologiaRESUMO
To evaluate the role of small mammals as hosts of severe fever with thrombocytopenia syndrome virus (SFTSV), we tested serum samples from rodents and shrews in China, collected in 2013. SFTSV antibodies and RNA were detected, suggesting that rodents and shrews might be hosts for SFTSV.
Assuntos
Doenças dos Animais/epidemiologia , Doenças dos Animais/virologia , Infecções por Bunyaviridae/veterinária , Roedores , Musaranhos , Doenças dos Animais/história , Animais , China/epidemiologia , História do Século XXI , Dados de Sequência Molecular , Phlebovirus , Filogenia , Prevalência , RNA ViralRESUMO
SFTSV, a tick-borne bunyavirus causing a severe hemorrhagic fever termed as severe fever with thrombocytopenia syndrome (SFTS). To evaluate the potential role of rodents and its ectoparasitic chiggers in the transmission of SFTSV, we collected wild rodents and chiggers on their bodies from a rural area in Qingdao City, Shandong Province, China in September 2020. PCR amplification of the M and L segments of SFTSV showed that 32.3% (10/31) of rodents and 0.2% (1/564) of chiggers (Leptotrombidium deliense) from the rodents were positive to SFTSV. Our results suggested that rodents and chiggers may play an important role in the transmission of SFTSV, although the efficiency of chiggers to transmit SFTSV needs to be further investigated experimentally.
Assuntos
Infecções por Bunyaviridae , Infestações por Ácaros , Phlebovirus , Carrapatos , Trombiculidae , Animais , China/epidemiologia , Febre , Phlebovirus/genética , RoedoresAssuntos
Infecções por Hantavirus/veterinária , Orthohantavírus/genética , Doenças dos Roedores/epidemiologia , Animais , China/epidemiologia , Reservatórios de Doenças/virologia , Genes Virais , Infecções por Hantavirus/diagnóstico , Infecções por Hantavirus/epidemiologia , Técnicas de Diagnóstico Molecular , Doenças dos Roedores/virologia , Roedores/virologiaRESUMO
Anaplasma are tick-borne obligatory intracellular bacteria, which infect humans and other animals. The Anaplasma species carried by ticks in China are not well studied. We collected 3145 questing Haemaphysalis longicornis ticks including 120 larvae, 2460 nymphs and 565 adults from vegetation in Jiaonan County, Shandong Province, China from 2013 to 2015. All ticks were examined for the presence of Anaplasma species by nested PCR amplification of the 16S rRNA gene. For further differentiation of A. capra from A. centrale, gltA and msp2 genes were sequenced for A. capra. Three Anaplasma species were detected in the nymph and/or adult ticks with the minimum infection rate of ticks as follows: 1.55% for A. bovis, 0.10% for A. phagocytophilum, and 0.03% for A. capra. These results indicated that the H. longicornis tick in Jiaonan County carried multiple Anaplasma species, which may be a challenge for public health in the studying area.
Assuntos
Anaplasma/isolamento & purificação , Anaplasmose/epidemiologia , Carrapatos/microbiologia , Anaplasma/genética , Anaplasmose/microbiologia , Animais , China/epidemiologia , Feminino , Florestas , Larva/microbiologia , Masculino , Ninfa/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência , Infestações por Carrapato/epidemiologia , Doenças Transmitidas por Carrapatos/epidemiologiaRESUMO
Severe fever with thrombocytopenia syndrome, an emerging hemorrhagic fever, is caused by severe fever with thrombocytopenia syndrome virus (SFTSV), a tick-borne bunyavirus. Information regarding SFTSV animal hosts is very limited. In this study, we showed that 64% (9/14) of hedgehogs in Shandong Province, China were seropositive to SFTSV antibody, suggesting that hedgehog could be a vertebrate parasitifer for SFTSV.
Assuntos
Infecções por Bunyaviridae/veterinária , Ouriços/virologia , Phlebovirus , Animais , Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , China/epidemiologia , Reservatórios de Doenças/veterinária , Ouriços/sangue , Estudos SoroepidemiológicosRESUMO
INTRODUCTION: Recently, hantaviruses have been discovered in insectivores in Europe, Asia, Africa, and North America. Imjin virus (MJNV) was first isolated from the lung tissues of Ussuri white-toothed shrew (Crocidura lasiura) from South Korea in 2009. We aim to detect the species and prevalence of insectivore- and rodent-borne hantaviruses in shrews and rodents. MATERIALS AND METHODS: Shrews and rodents were captured in Jiaonan County of Shandong Province, China, in 2014. RT-PCR was used to amplify viral RNA of Hantavirus species, including insectivore-borne Imjin virus (MJNV), rodent-borne Hantaan virus (HTNV), and Seoul virus (SEOV) from shrews and rodents. RESULTS AND DISCUSSION: We found that MJNV infected 10.7% (19/178) of Crocidura shrews, but it infected none of rodents (0/475); we also found that 2 of 178 (1.1%) Crocidura shrews were PCR positive to SEOV. This study indicated that the major animal hosts of Imjin virus are shrews, and rodent-borne SEOV can infect shrews.