Australian vertebrate hosts of Japanese encephalitis virus; a review of the evidence.

Japanese Encephalitis Virus (JEV) transmission in temperate Australia has underscored a critical need to characterise transmission pathways and identify probable hosts of infection within the country. This systematic review consolidates existing research on the vertebrate hosts of JEV that are known to exist in Australia. Specifically, we aim to identify probable species for JEV transmission, their potential role as either a spillover or maintenance host and identify critical knowledge gaps. Data were extracted from studies involving experimental infection, seroprevalence, and virus isolation and were available for 22 vertebrate species known to reside in Australia. A host competence score was calculated to assess the potential for a given species to infect JEV vectors and to quantity their possible role in JEV transmission. Based on the host competence score and ecology of each species, we find ardeid birds, feral pigs, and flying foxes have potential as maintenance hosts for JEV in the Australian context. We also note that brushtail possums and domestic pigs have potential as spillover hosts under certain outbreak conditions. However, evidence to confirm these roles in localized transmission or outbreaks is sparse, emphasizing the need for further targeted research. This review provides a foundation for future investigations into JEV transmission in Australia, advocating for enhanced surveillance and standardized research methodologies to better understand and mitigate the virus's impact.

Amphibian infection tolerance to chytridiomycosis.

Animal defences against infection involve two distinct but complementary mechanisms: tolerance and resistance. Tolerance measures the animal's ability to limit detrimental effects from a given infection, whereas resistance is the ability to limit the intensity of that infection. Tolerance is a valuable defence for highly prevalent, persistent or endemic infections where mitigation strategies based on traditional resistance mechanisms are less effective or evolutionarily stable. Selective breeding of amphibians for enhanced tolerance to Batrachochytrium spp. has been suggested as a strategy for mitigating the impacts of the fungal disease, chytridiomycosis. Here, we define infection tolerance and resistance in the context of chytridiomycosis, present evidence for variation in tolerance to chytridiomycosis, and explore epidemiological, ecological and evolutionary implications of tolerance to chytridiomycosis. We found that exposure risk and environmental moderation of infection burdens are major confounders of resistance and tolerance, chytridiomycosis is primarily characterized by variation in constitutive rather than adaptive resistance, tolerance is epidemiologically important in driving pathogen spread and maintenance, heterogeneity of tolerance leads to ecological trade-offs, and natural selection for resistance and tolerance is likely to be dilute. Improving our understanding of infection tolerance broadens our capacity for mitigating the ongoing impacts of emerging infectious diseases such as chytridiomycosis. This article is part of the theme issue 'Amphibian immunity: stress, disease and ecoimmunology'.

Multiple physiological cohorts comprise seasonal activity of wild Amblyomma americanum (Acari: Ixodidae) nymphs.

Nymphs of the hard tick Amblyomma americanum (L.) are an important life stage in the maintenance and transmission of tick-borne pathogens. As pathogen composition can vary across developmental cohorts, it is essential to understand the demographic structure of the questing population. Amblyomma americanum nymphs often display a second peak in activity during late summer, but it is unknown whether this peak represents older overwintered ticks or younger newly molted ticks. The objective of this study was to examine the heme concentration in field-captured A. americanum nymphs to determine if the questing population consists of one physiological cohort or multiple cohorts in a season. Ticks were collected from March to August in an old field of primarily non-native grasses, and heme concentration was used to assess physiological age. LOESS modeling depicted that heme concentration in the population declined from March to early July but increased in later sampling sessions. As ticks cannot replenish declining heme stores without a blood meal, a late-summer spike in heme concentration demonstrates that newly molted nymphs are entering the active population. The vector potential of these newly emerging nymphs may differ from those collected earlier in the year as pathogen diversity depends on reservoir host dynamics and timing of larval feeding.

Seasonal changes in questing efficiency of wild Amblyomma americanum (Acari: Ixodidae) nymphs.

Understanding the factors which influence host-seeking behavior of ticks is essential to determine the risk they pose as a vector of pathogens. While many studies have evaluated the impact of environmental variables on tick behavior, few have examined how seasonal changes in physiological status may further modify patterns of activity. In this study, we measured differences in questing behavior of mid spring- and early summer-caught Amblyomma americanum nymphs held under standardized laboratory conditions. As both groups represent the same cohort of overwintered nymphs, we hypothesized that age-related changes in the older summer ticks may influence questing behavior. In each season, we collected nymphs from field and forest habitats in northeast Missouri, after which we placed each nymph individually in a desiccating vertical questing apparatus with a hydrating microenvironment at the base. On the day following collection, we recorded the height of each nymph in the apparatus bi-hourly from 04:00 to 22:00 and calculated the vertical displacement between consecutive observations. Despite displaying no differences in mean questing height throughout the experiment, active ticks collected in the summer group (n = 89) travelled greater cumulative distances between desiccating and hydrating microenvironments than those collected in the spring (n = 119). This suggests that questing efficiency decreases in summer nymphs to accommodate increased time allocation towards rehydration. While we observed no direct association between body size and distance travelled, body size of the nymphal population also decreased significantly from spring to summer. Overall, our results demonstrate that there are seasonal changes in how A. americanum responds to environmental conditions. To more accurately predict host-seeking behavior of ticks across seasons, models should incorporate physiological parameters of the active ticks in a given population.