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4.
J Clin Microbiol ; 5(4): 488-9, 1977 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-300741

RESUMO

A new differential medium for isolation of Corynebacterium vaginale is described. This opaque medium containing 1% corn starch allows detection of C. vaginale by the zones of clearing developing around the colonies.


Assuntos
Meios de Cultura , Gardnerella vaginalis/isolamento & purificação , Haemophilus/isolamento & purificação , Anaerobiose , Estudos de Avaliação como Assunto , Gardnerella vaginalis/crescimento & desenvolvimento , Amido , Zea mays
5.
J Clin Microbiol ; 33(5): 1141-4, 1995 May.
Artigo em Inglês | MEDLINE | ID: mdl-7615718

RESUMO

Bacterial antigen testing (BAT) of cerebrospinal fluid (CSF) by latex agglutination is a low-yield procedure in patients whose CSF specimens have normal laboratory parameters. Between August 1992 and August 1994, we evaluated 287 bacterial antigen (BA) test requests to determine whether yields could be improved and whether patient costs could be reduced by cancelling BAT for those patients with normal CSF parameters (cell count, protein, glucose) after consultation with physicians. A total of 171 (68%) BA tests were canceled by this approach. None of these CSF specimens was culture positive for an organism detectable by BAT. Of the remaining 116 CSF specimens tested, only 3 were positive by BAT, one each for Neisseria meningitidis, Streptococcus pneumoniae, and group B streptococcus. Only 43 of the CSF specimens tested had at least two abnormal parameters; the 3 positive CSF specimens were included in this group. In light of the low rate of positivity, the number of BA tests can be further reduced by establishing criteria that must be met before a CSF specimen is accepted for BAT. After review of our data and the literature concerning this topic, we concluded that only specimens with leukocyte counts of > or = 50 cells per mm3 should be tested. Of 287 specimens evaluated in our study, only 36 met this criterion, including the 3 BA-positive specimens. Enacting such a restriction would have reduced the total number of BA tests by 251 (87%) without compromising patient care. A laboratory cost savings of $6,500 per year would have been realized, with a substantial reduction in the cost per positive test. Patient charges would have been reduced by $12,500 per year.


Assuntos
Antígenos de Bactérias/líquido cefalorraquidiano , Testes de Fixação do Látex/normas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise Custo-Benefício , Estudos de Avaliação como Assunto , Humanos , Testes de Fixação do Látex/economia , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/economia , Meningites Bacterianas/microbiologia , Garantia da Qualidade dos Cuidados de Saúde
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