RESUMO
Endometriosis is a common disease in women of childbearing age and is closely associated with female infertility. However, the pathogenesis of endometriosis-related infertility is still not fully understood. Prohibitin 1 (PHB1), a highly conserved protein related to mitochondrial function, is differentially expressed in the endometrium of patients with endometriosis. However, the role of PHB1 in glucose metabolism in granulosa cells remains unclear. In this study, we investigated whether PHB1 expression and glucose metabolism patterns differ in the granulosa cells of patients with endometriosis and those of patients serving as controls. We then evaluated these changes after PHB1 was upregulated or downregulated in the human granulosa cell line (KGN) using a lentivirus construct. In the granulosa cells of patients with endometriosis, significantly elevated PHB1 expression, increased glucose consumption and lactic acid production, as well as aberrant expression of glycolysis-related enzymes were found compared to those without endometriosis (P < 0.05). After PHB1 expression was upregulated in KGN cells, and the expression of enzymes related to glucose metabolism, glucose consumption and lactic acid production was strikingly increased compared to controls (P < 0.05). The opposite results were found when PHB1 expression was downregulated in KGN cells. Additionally, the cell proliferation and apoptosis rates, ATP synthesis, reactive oxygen species (ROS) levels and mitochondrial membrane potential (MMP) were significantly altered after down-regulation of PHB1 expression in KGN cells (P < 0.05). This study suggested that PHB1 plays a pivotal role in mitigating the loss of energy caused by impaired mitochondrial function in granulosa cells of patients with endometriosis, which may explain, at least in part, why the quality of oocytes in these patients is compromised.
Assuntos
Endometriose , Glucose , Células da Granulosa , Infertilidade , Proibitinas , Endometriose/genética , Endometriose/metabolismo , Endometriose/patologia , Feminino , Glucose/metabolismo , Células da Granulosa/metabolismo , Células da Granulosa/patologia , Humanos , Infertilidade/genética , Infertilidade/metabolismo , Infertilidade/patologia , Ácido Láctico/metabolismo , Proibitinas/biossíntese , Proibitinas/genética , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
In this study, a micro helium dielectric barrier discharge (µHDBD) plasma device fabricated using 3D printing and molding techniques was coupled with a mini spectrometer to detect and identify different classes of volatile organic compounds (VOCs) using optical emission spectrometry (OES). We tested 11 VOCs belonging to three different classes (straight-chain alkanes, aromatics, and polar organic compounds). Our results clearly demonstrate that the optical emission spectra of different classes of VOCs show clear differences, and therefore, can be used for identification. Additionally, the emission spectra of VOCs with a similar structure (such as n-pentane, n-hexane, n-heptane, n-octane, and n-nonane) have similar optical emission spectrum shape. Acetone and ethanol also have similar emission wavelengths, but they show different line intensities for the same concentrations. We also found that the side-chain group of aromatics will also affect the emission spectra even though they have a similar structure (all have a benzene ring). Moreover, our µHDBD-OES system can also identify multiple compounds in VOC mixtures. Our work also demonstrates the possibility of identifying different classes of VOCs by the OES shape.
RESUMO
In this paper, we report on a printable glass-based manufacturing method and a new proof-of-concept colorimetric signal readout scheme for a dielectric barrier discharge (DBD)-type helium plasma photoionization detector. The sensor consists of a millimeter-sized glass chamber manufactured using a printable glass suspension. Plasma inside the chip is generated using a custom-built power supply (900 V and 83.6 kHz), and the detector uses â¼5 W of power. Our new detection scheme is based on detecting the change in the color of plasma after the introduction of target gases. The change in color is first captured by a smartphone camera as a video output. The recorded video is then processed and converted to an image light intensity vs retention time plot (gas chromatogram) using three standard color space models (red, green, blue (RGB), hue, saturation, lightness (HSL), and hue, saturation, value (HSV)) with RGB performing the best among the three models. We successfully detected three different categories of volatile organic compounds using our new detection scheme and a 30-m-long gas chromatography column: (1) straight-chain alkanes (n-pentane, n-hexane, n-heptane, n-octane, and n-nonane), (2) aromatics (benzene, toluene, and ethylbenzene), and (3) polar compounds (acetone, ethanol, and dichloromethane). The best limit of detection of 10 ng was achieved for benzene at room temperature. Additionally, the device showed excellent performance for different types of sample mixtures consisting of three and five compounds. Our new detector readout method combined with our ability to print complex glass structures provides a new research avenue to analyze complex gas mixtures and their components.
RESUMO
Aqueous Zn ion batteries are promising systems due to their intrinsic safety, low cost, and non-toxicity, and the Zn corrosion and dendrite growth will cause the poor reversibility of Zn anode. Herein, the porous Zn@C solid, hollow, and yolk-shell microsphere films are developed as Zn anode antifluctuator (ZAAF). The prepared yolk-shell microspheres (Zn@C yolk-shell microsphere [ZCYSM]) film with superior buffering can effectively restrict the deposition of Zn metal in its interior and inhibit the volume expansion during plating/stripping process, thus modulating the Zn2+ flux and enabling stable Zn cycling. As a proof of concept, the ZCYSM@Zn symmetric cells achieve the excellent cyclic stability over 4000 h and cumulative plated capacity of 4 Ah cm-2 at a high current density of 10 mA cm-2 . Concomitantly, the suppressed corrosion reactions and dendrite-free ZAAF significantly improve the durability of full cells (coupled to CaV6 O16 ·3H2 O). Additionally, durable pouch cell and electrochemical neuromorphic inorganic device (ENIDe) are integrated to simulate neural network, providing a strategy for extreme interconnectivity comparable to the human brain.
RESUMO
PURPOSE: Oseltamivir is a prodrug that requires metabolic activation but there is little information on whether natural health products interact to prevent the biotransformation by the carboxylesterase. METHODS: HPLC-DAD-ESI-MSD and fluorometric assays were used to determine if 50-pooled mixed gender human liver microsomes can mediate the formation of the active carboxylate metabolite and then if this reaction is affected by natural health products. RESULTS: Extracts from 6 traditional Cree botanicals, a commercially available Echinacea product, Goldenseal and a traditional Chinese medicine reduced the formation of the active drug. In addition to oseltamivir carboxylate we report the detection of two new metabolites which are derivatives of oseltamivir carboxylate, one of which is a metabonate formed as a result of methanol. CONCLUSIONS: In vitro studies would suggest that there is the potential for some natural health products used by patients in response to pandemic A/H1N1 to reduce drug efficacy. Further studies are required to determine if these potential interactions could be clinically significant.
Assuntos
Antivirais/metabolismo , Interações Ervas-Drogas , Microssomos Hepáticos/metabolismo , Oseltamivir/análogos & derivados , Produtos Biológicos/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Feminino , Fluorometria , Humanos , Masculino , Medicina Tradicional Chinesa , Oseltamivir/metabolismo , Extratos Vegetais/farmacologia , Pró-Fármacos , Espectrometria de Massas por Ionização por Electrospray/métodosRESUMO
An alteration in the secondary metabolism of maize (Zea mays L.) genetically modified with the wheat oxalate oxidase (OxO) gene was observed using HPLC and fluorescence microscopy. Phenolic concentrations in the OxO lines were significantly increased, but DIMBOA synthesis was reduced due to a diversion in the shikimate pathway leading to phenolic and hydroxamic acids. Ferulic acid exhibited the largest increase and accounted for 80.4% of the total soluble phenolics. Transcription of a 13-lipoxygenase gene, coding for a key enzyme involved in the regulation of secondary metabolism, was substantially higher in the OxO line than in the null line. To test whether the high levels of soluble phenolic acids, in particular ferulic acid, contributed to the insect resistance in the OxO maize, ferulic acid was administered in meridic diets to European corn borer (ECB). A significant negative correlation between ferulic acid concentration and ECB larval growth rate was found. Field testing during 2001 showed that OxO maize was more resistant to ECB, with leaf consumption and stalk-tunneling damage significantly reduced by 28-34 and 37-39%, respectively, on all of the OxO lines tested and confirming published 2000 findings.
Assuntos
Lepidópteros/fisiologia , Oxirredutases/genética , Plantas Geneticamente Modificadas/metabolismo , Zea mays/enzimologia , Zea mays/metabolismo , Animais , Benzoxazinas/análise , Oxirredutases/metabolismo , Fenóis/análise , Doenças das Plantas , Triticum/enzimologia , Triticum/genética , Zea mays/químicaRESUMO
BACKGROUND: Goji berry (Lycium barbarum) has been used as traditional Chinese medicine and a functional food in China. Goji tea may interact with drugs such as warfarin by inhibiting the cytochrome P450 (CYP) 2C9, and this study was undertaken to characterize the effect of Goji products on CYP2C9/19-, CYP2D6 *1/*10-, CYP3A4/5/7-, CYP19-, and flavin-containing monooxygenase (FMO) 3-mediated metabolism. METHODS: Goji juice, water, and ethanol extracts were examined for their effect on CYP2C9/19-, 2D6-, 3A4/5/7-, 4A11-, CYP19-, and FMO3-mediated metabolism by using in vitro bioassay. The mechanism-based inactivation (MBI) of Goji juice on CYP3A4 was also examined. RESULTS: Data indicates that both fresh juice and commercially available juice caused strong inhibition (over 75â%) of most of the major CYP450 enzymes and moderate inhibition of FMO3 (30-60â%). Compared to juice, the Goji cold/hot water extracts effected low inhibition (below 30â%) of these enzymes. Ethanol (80â%) extracts exhibit the strongest inhibition on CYP2C9 and 2C19 (over 90â%). The inhibition pattern of dried and fresh berry extract and high-performance liquid chromatography (HPLC)-UV fingerprints were similar. CONCLUSIONS: These findings suggest that Goji products (berries, tea, tincture, and juice) can inhibit phase I drug metabolism enzymes and have the potential to affect the safety and efficacy of therapeutic products.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Frutas , Interações Ervas-Drogas , Lycium , Desintoxicação Metabólica Fase I , Oxigenases/metabolismo , Extratos Vegetais/farmacologia , Humanos , Preparações de PlantasRESUMO
The potential for 15 different ales (6), ciders (2 apple and 1 pear), and porters (6) and 2 non-alcoholic products to affect cytochrome P450 (CYP)-mediated biotransformation and P-glycoprotein-mediated efflux of rhodamine was examined. As in our previous study, a wide range of recovered nonvolatile suspended solids dry weights were noted. Aliquots were also found to have varying effects on biotransformation and efflux. Distinct differences in product ability to affect the safety and efficacy of therapeutic products confirmed our initial findings that some porters (stouts) have a potential to affect the safety and efficacy of health products metabolized by CYP2D6 and CYP3A4 isozymes. Most products, except 2 of the ciders and the 2 non-alcoholic products, also have the potential to affect the safety of CYP2C9 metabolized medications and supplements. Further studies are required to determine the clinical significance of these findings.