RESUMO
Methylglyoxal was shown to impair adipose tissue capillarization and insulin sensitivity in obese models. We hypothesized that glyoxalase-1 (GLO-1) activity could be diminished in the adipose tissue of type 2 diabetic obese patients. Moreover, we assessed whether such activity could be increased by GLP-1-based therapies in order to improve adipose tissue capillarization and insulin sensitivity. GLO-1 activity was assessed in visceral adipose tissue of a cohort of obese patients. The role of GLP-1 in modulating GLO-1 was assessed in type 2 diabetic GK rats submitted to sleeve gastrectomy or Liraglutide treatment, in the adipose tissue angiogenesis assay and in the HUVEC cell line. Glyoxalase-1 activity was decreased in visceral adipose tissue of pre-diabetic and diabetic obese patients, together with other markers of adipose tissue dysfunction and correlated with increased HbA1c levels. Decreased adipose tissue GLO-1 levels in GK rats were increased by sleeve gastrectomy and Liraglutide, being associated with overexpression of angiogenic and vasoactive factors, as well as insulin receptor phosphorylation (Tyr1161). Moreover, GLP-1 increased adipose tissue capillarization and HUVEC proliferation in a glyoxalase-dependent manner. Lower adipose tissue GLO-1 activity was observed in dysmetabolic patients, being a target for GLP-1 in improving adipose tissue capillarization and insulin sensitivity.
Assuntos
Tecido Adiposo/irrigação sanguínea , Capilares/efeitos dos fármacos , Diabetes Mellitus Tipo 2/tratamento farmacológico , Receptor do Peptídeo Semelhante ao Glucagon 1/agonistas , Hipoglicemiantes/farmacologia , Incretinas/farmacologia , Resistência à Insulina , Lactoilglutationa Liase/metabolismo , Liraglutida/farmacologia , Neovascularização Fisiológica/efeitos dos fármacos , Adulto , Idoso , Animais , Capilares/enzimologia , Capilares/fisiopatologia , Células Cultivadas , Diabetes Mellitus Tipo 2/enzimologia , Diabetes Mellitus Tipo 2/fisiopatologia , Modelos Animais de Doenças , Feminino , Gastrectomia , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Receptor do Peptídeo Semelhante ao Glucagon 1/metabolismo , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/enzimologia , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/enzimologia , Obesidade/fisiopatologia , Obesidade/cirurgia , Ratos Wistar , Transdução de SinaisRESUMO
BACKGROUND: the metabolic syndrome (MS) is a public health problem in Mexico and in the world due to its progressive growing and cardiovascular complications. Our aim was to identify the frequency of MS in health care workers in a primary care unit. METHODS: a prospective study, descriptive, observational and transversal was performed. A non probabilistic sample was obtained in agreement with health 90 workers (family physicians, dentist, office medical assistants, nurses, laboratory personnel and social workers), that were working from March to September of 2007. The Adult Treatment Panel III criterion was used to diagnose and classify. RESULTS: the 40 % presented MS, it was higher in women (42 %) and in the group of 40 to 49 years (47 %). Dentists and social workers were the most affected (62 %), the nurses (42 %), family physicians (32 %) and medical office assistant (30 %). CONCLUSIONS: the MS was common in the health personnel. Our results showed major frequency compared with that reported in other countries and general Mexican population of adults.