RESUMO
ization and unification of diseases of the oral mucosa in patients who underwent Covid-19, in order to increase the effectiveness of qualified dental care for this group of people in a pandemic and create conditions for continuous professional development of dentists. 42 patients were attended, who came through Covid-19, aged 36 to 70 years, who applied independently or were referred by doctors of private clinics in the city of Ternopil. The gender distribution of patients was 19 males and 23 females. Clinical examination of patients with acute respiratory viral infection Сovid-19, at the stage of subjective methods, revealed the following complaints: loss of taste was found in 38 people (90%) patients, salivation disorders (dry mouth or hypersalivation) - in 40 people (95%) patients, burning sensation, discomfort and «creeping ants¼ in the tongue or other anatomical components of the oral mucosa in 29 people (69%) patients. Complaints about the integrity of the oral mucosa and the associated pain were associated with pain in 13 people (31%). Patients diagnosed with Covid-19 were diagnosed with glossodynia 7 individuals, (17%), candidal stomatitis 12 individuals, (28%), herpetic stomatitis 5 individuals, (12%), and chronic recurrent aphthous stomatitis 8 individuals, (19%), desquamative glossitis in 10 people (24%). In some clinical cases, 10 people (24%) observed a combination of nosological units. The obtained results substantiate the development of the necessary algorithm of dental examination of dental data of patients in a pandemic.
Assuntos
COVID-19 , Mucosa Bucal , Odontólogos , Feminino , Humanos , Masculino , Pandemias , SARS-CoV-2 , Organização Mundial da SaúdeRESUMO
Different allelic variants of genes that encode ATP-sensitive potassium (KATP ) channels' subunits may contribute to the development of heart failure. The purpose of the work to investigate SNPs in genes that encode KATP channels in relation to echocardiographic parameters in chronic heart failure (CHF) patients. Ninety-nine people with CHF of ischemic origin with left ventricular systolic dysfunction were examined. The control group is represented by 108 clinically healthy subjects. KCNJ11 polymorphisms Ile337Val and Glu23Lys, and ABCC8 polymorphism Ser1369Ala were genotyped using polymerase chain reaction. In CHF patients, the frequency of the Ile337Val genotype was: Ile/Ile, 40.4%; Ile/Val, 45.5%; and Val/Val, 14.1%. The patients with the Val/Val genotype had left ventricular (LV) mass that was 334.15 g, which was 27.3% (P < 0.05) lower versus Ile/Val patients (425.48 g). The index of this parameter was also significantly lower (28.4%, P < 0.05). In CHF patients, the frequency of Glu23Lys and Ser1369Ala was: Glu/Glu and Ser/Ser, 43.4%; heterozygote, 44.4%; Lys/Lys and Ala/Ala, 12.2%. The patients with the Lys/Lys and Ala/Ala genotypes had a significantly lower LV mass index and LV end-diastolic volume (22.9% and 26.8%, P < 0.05) versus heterozygotes. Thus, the greatest LV mass and LV end-diastolic volume values are associated with heterozygotes, while the smallest are associated with minor homozygotes.
Assuntos
Ecocardiografia , Insuficiência Cardíaca/genética , Canais de Potássio Corretores do Fluxo de Internalização/genética , Receptores de Sulfonilureias/genética , Idoso , Alelos , Estudos de Casos e Controles , Feminino , Frequência do Gene , Genótipo , Insuficiência Cardíaca/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Ucrânia , Disfunção Ventricular Esquerda/genéticaRESUMO
This corrects the article DOI: 10.1103/PhysRevLett.117.182502.
RESUMO
We utilize various ab initio approaches to search for a low-lying resonance in the four-neutron (4n) system using the JISP16 realistic NN interaction. Our most accurate prediction is obtained using a J-matrix extension of the no-core shell model and suggests a 4n resonant state at an energy near E_{r}=0.8 MeV with a width of approximately Γ=1.4 MeV.
RESUMO
The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca2+,Mg2+-ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this subcellular fraction. The inhibition coefficient I0.5 values were 20.2 ± 0.5 and 58.5 ± 6.4 µM for the membrane fraction and the solubilized enzyme, respectively. The inhibitory effect of calix[4]arene C-90 was selective comparatively to other ATPases localized in the plasma membrane: calix[4]arene C-90 did not influence the activities of Na+,K+-ATPase and "basal" Mg2+-ATPase. The inhibitory effect of calix[4]arene C-90 on the Ca2+,Mg2+-ATPase activity was associated with the cooperative action of four trifluoromethylphenylsulfonylimine (sulfonylamidine) groups oriented similarly on the upper rim of the calix[4]arene macrocycle (the calix[4]arene "bowl"). The experimental findings seem to be of importance for studies, using calix[4]arene C-90, of membrane mechanisms of regulation of calcium homeostasis in smooth muscle cells and also for investigation of the participation of the plasma membrane Ca2+-pump in control of electro- and pharmacomechanical coupling in myocytes.
Assuntos
ATPase de Ca(2+) e Mg(2+)/antagonistas & inibidores , Calixarenos/química , Membrana Celular/enzimologia , Miométrio/metabolismo , Fenóis/química , Animais , ATPase de Ca(2+) e Mg(2+)/metabolismo , Calixarenos/síntese química , Calixarenos/metabolismo , Membrana Celular/metabolismo , Feminino , Cinética , Miócitos de Músculo Liso/enzimologia , Miócitos de Músculo Liso/metabolismo , Miométrio/citologia , Fenóis/síntese química , Fenóis/metabolismo , Ligação Proteica , SuínosRESUMO
Andreeva Bay in northwest Russia hosts one of the former coastal technical bases of the Northern Fleet. Currently, this base is designated as the Andreeva Bay branch of Northwest Center for Radioactive Waste Management (SevRAO) and is a site of temporary storage (STS) for spent nuclear fuel (SNF) and other radiological waste generated during the operation and decommissioning of nuclear submarines and ships. According to an integrated expert evaluation, this site is the most dangerous nuclear facility in northwest Russia. Environmental rehabilitation of the site is currently in progress and is supported by strong international collaboration. This paper describes how the optimization principle (ALARA) has been adopted during the planning of remediation work at the Andreeva Bay STS and how Russian-Norwegian collaboration greatly contributed to ensuring the development and maintenance of a high level safety culture during this process. More specifically, this paper describes how integration of a system, specifically designed for improving the radiological safety of workers during the remediation work at Andreeva Bay, was developed in Russia. It also outlines the 3D radiological simulation and virtual reality based systems developed in Norway that have greatly facilitated effective implementation of the ALARA principle, through supporting radiological characterisation, work planning and optimization, decision making, communication between teams and with the authorities and training of field operators.
Assuntos
Locais de Resíduos Perigosos , Imageamento Tridimensional/métodos , Modelos Organizacionais , Cultura Organizacional , Proteção Radiológica/métodos , Resíduos Radioativos/prevenção & controle , Gestão da Segurança/organização & administração , Descontaminação/métodos , Noruega , Monitoramento de Radiação/métodos , Federação RussaRESUMO
Chronic (15 days) single daily intraperitoneal insertion of the new preparation MT (5 mg/kg) and metoprolol (10 mg/kg) into SHR rats leads to the same decrease (18%) in arterial pressure. In addition, MT exhibits a cardioprotective effect because of NO-mimetic properties, increasing NO formation in myocardium via increasing general NOS activity and eNOS expression. MT normalizes iNOS expression in myocardium mitochondria and decreases nitrotyrosine (nitrosation stress marker) formation. At the same time, the reference preparation metoprolol did not exhibit NO-mimetic properties in myocardium of SHR rats.
Assuntos
Antiarrítmicos/farmacologia , Cardiotônicos/farmacologia , Metoprolol/farmacologia , Óxido Nítrico/metabolismo , Triazóis/farmacologia , Animais , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Miocárdio/enzimologia , Óxido Nítrico Sintase Tipo III/biossíntese , Ratos , Ratos Endogâmicos SHRRESUMO
The article presents data regarding links of I/D angiotensin-converting enzyme (ACE) gene polymorphism and structural changes in the myocardium and predictive value in chronic heart failure (CHF). We did not find association of I/D ACE gene polymorphism and structural changes in the myocardium and predictive value in patients with CHF, receiving treatment inhibitor ACE.
Assuntos
Insuficiência Cardíaca/genética , Hipertensão/genética , Peptidil Dipeptidase A/genética , Polimorfismo Genético , Sistema Renina-Angiotensina/efeitos dos fármacos , Idoso , Substituição de Aminoácidos , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Inibidores da Enzima Conversora de Angiotensina/uso terapêutico , Anti-Hipertensivos/farmacologia , Anti-Hipertensivos/uso terapêutico , Ácido Aspártico/genética , Feminino , Genótipo , Insuficiência Cardíaca/tratamento farmacológico , Insuficiência Cardíaca/mortalidade , Insuficiência Cardíaca/patologia , Humanos , Hipertensão/tratamento farmacológico , Hipertensão/mortalidade , Hipertensão/patologia , Isoleucina/genética , Masculino , Pessoa de Meia-Idade , Miocárdio/patologia , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Prognóstico , Sistema Renina-Angiotensina/genética , Análise de SobrevidaRESUMO
Apoptosis, a physiologically critical process, is characterized by a destruction of the cell after sequential degradation of key cellular components. Here, we set out to explore the fate of the physiologically indispensable nuclear envelope (NE) in this process. The NE mediates the critical nucleocytoplasmic transport through nuclear pore complexes (NPCs). In addition, the NE is involved in gene expression and contributes significantly to the overall structure and mechanical stability of the cell nucleus through the nuclear lamina, which underlies the entire nucleoplasmic face of the NE and thereby interconnects the NPCs, the NE, and the genomic material. Using the nano-imaging and mechanical probing approach atomic force microscopy (AFM) and biochemical methods, we unveiled the fate of the NE during apoptosis. The doomed NE sustains a degradation of both the mediators of the critical selective nucleocytoplasmic transport, namely NPC cytoplasmic filaments and basket, and the nuclear lamina. These observations are paralleled by marked softening and destabilization of the NE and the detection of vesicle-like nuclear fragments. We conclude that destruction of the cell nucleus during apoptosis proceeds in a strategic fashion. Degradation of NPC cytoplasmic filaments and basket shuts down the critical selective nucleocytoplasmic cross-talk. Degradation of the nuclear lamina disrupts the pivotal connection between the NE and the chromatin, breaks up the overall nuclear architecture, and softens the NE, thereby enabling the formation of nuclear fragments at later stages of apoptosis.
Assuntos
Apoptose/fisiologia , Cromatina/metabolismo , Lâmina Nuclear/metabolismo , Poro Nuclear/metabolismo , Transporte Ativo do Núcleo Celular/fisiologia , Animais , Células Cultivadas , Microscopia de Força Atômica/métodos , Lâmina Nuclear/ultraestrutura , Poro Nuclear/ultraestrutura , Xenopus laevisRESUMO
The SPASIBA force field has been applied to the determination of the structure and dynamical properties of various disaccharides. It has been shown that the experimental properties (structure, dipole moment, conformational relative energies) are satisfactorily predicted. The anomeric and exo-anomeric effects are confidently reproduced without specific terms for the alpha and beta anomers and the type of glycosidic linkages.
Assuntos
Algoritmos , Configuração de Carboidratos , Dissacarídeos/química , Glicosídeos/química , Software , Eletricidade Estática , Estereoisomerismo , Relação Estrutura-Atividade , Compostos de Sulfidrila/química , Termodinâmica , Trealose/químicaRESUMO
The neuroleptic, analgesic, antiinflammatory, antihypoxic, diuretic, bacteriostatic, and fungistatic activity of bicyclic noncondensed thiazolidons-2 with alkylene and aryl bridges was studied. Certain regularities in the relations between the structure of the compounds under study and their biological activity were found.
Assuntos
Tiazóis/farmacologia , Analgésicos/farmacologia , Analgésicos/toxicidade , Animais , Anti-Infecciosos/farmacologia , Anti-Infecciosos/toxicidade , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/toxicidade , Antifúngicos/farmacologia , Antifúngicos/toxicidade , Antipsicóticos/farmacologia , Antipsicóticos/toxicidade , Diuréticos/farmacologia , Diuréticos/toxicidade , Feminino , Masculino , Camundongos , Ratos , Relação Estrutura-Atividade , Tiazóis/toxicidadeRESUMO
It is established that KS-79 compound possesses the high level of antioxidant activity and realizes its antioxidant effect at initial stages of free-radical hydrogen peroxide oxidation of lipids. Due to this ability it differs from the oxidants of direct-type action.
Assuntos
Antioxidantes/farmacologia , Hidrazinas/farmacologia , Hipóxia Encefálica/tratamento farmacológico , Quinazolinas/farmacologia , Animais , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/metabolismo , Radicais Livres , Hipóxia Encefálica/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Quinazolinonas , Ratos , Ratos Wistar , Vitamina E/metabolismoRESUMO
The new antioxidant Thiotriazoline was used in the treatment of patients with retinal vascular occlusive processes. The agent improved retinal metabolism by inhibiting free radical oxidation. Thiotriazoline is superior to alpha-tocopherol acetate in its activity. Unlike the latter, it has no vasodilating effect.
Assuntos
Antioxidantes/uso terapêutico , Olho/irrigação sanguínea , Isquemia/tratamento farmacológico , Triazóis/uso terapêutico , Doença Aguda , Avaliação de Medicamentos , Quimioterapia Combinada , Radicais Livres , Humanos , Isquemia/sangue , Nervo Óptico/irrigação sanguínea , Doenças do Nervo Óptico/sangue , Doenças do Nervo Óptico/tratamento farmacológico , Oxirredução/efeitos dos fármacos , Doenças Retinianas/sangue , Doenças Retinianas/tratamento farmacológico , Veia Retiniana , Trombose/sangue , Trombose/tratamento farmacológicoRESUMO
Antioxidant and antiradical activity of 1,2,4-triazole and quinazoline derivatives inhibiting superradical at the initial stages of free-radical oxidation have been studied in vitro by the method of Hara P, Mista, 1972. It is acceptable for the determination of antiradical and antioxidant activity of newly synthesized compounds.
Assuntos
Antioxidantes/uso terapêutico , Sequestradores de Radicais Livres/uso terapêutico , Ataque Isquêmico Transitório/tratamento farmacológico , Quinazolinas/uso terapêutico , Triazóis/uso terapêutico , Animais , Estrutura Molecular , Ratos , Ratos Wistar , Triazóis/químicaAssuntos
Barreira Hematoencefálica/efeitos dos fármacos , Transtornos Cerebrovasculares/tratamento farmacológico , Transtornos Cerebrovasculares/fisiopatologia , Hipertensão/tratamento farmacológico , Hipertensão/fisiopatologia , Animais , Antioxidantes/uso terapêutico , Hemorragia Cerebral/tratamento farmacológico , Hemorragia Cerebral/etiologia , Hemorragia Cerebral/fisiopatologia , Transtornos Cerebrovasculares/etiologia , Avaliação Pré-Clínica de Medicamentos , Humanos , Hidrazinas/uso terapêutico , Hipertensão/complicações , Masculino , Quinazolinas/uso terapêutico , Quinazolinonas , Ratos , Ratos WistarRESUMO
The effect of quinazopyrine on skin wound healing in rats was studied. Quinazopyrine was found to stimulate synthesis of nucleic acids, maturation of fibroblasts and to increase mechanical strength of the postoperative scar. This effect retains in animals with alloxan-induced diabetes. The wound-healing activity of quinazopyrine was higher than that of methyluracil, dimexide, pentoxyl, sodium nucleinate, potassium orotate and riboxine.
Assuntos
Quinazolinas/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Diabetes Mellitus Experimental/complicações , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Feminino , Dose Letal Mediana , Masculino , Camundongos , Quinazolinas/administração & dosagem , Quinazolinas/toxicidade , Ratos , Pele/efeitos dos fármacos , Fenômenos Fisiológicos da Pele , Infecções Cutâneas Estafilocócicas/tratamento farmacológico , Estimulação Química , Infecção dos Ferimentos/tratamento farmacológicoRESUMO
The changes in protein-protein interactions mediated by SH3 domain of the regulatory p85 alpha subunit of phosphatidylinositol 3-kinase (Pl 3-kinase) in the course of herbimycin A-induced erythroid differentiation of the human erythroleukemia cell line K562 have been analyzed. Binding assay was performed in vitro using a recombinant form of SH3 domain of p85 alpha, conjugated with glutathione-S-transferase. pTyr-containing 210, 116, 52 and 46 kDa proteins, binding of which are modulated in differentiated cells, were identified and binding dynamics was analysed. The obtained data on binding the specific pTyr-containing proteins with regulatory subunit of Pl 3-kinase testify about the coordinated control of Pl 3-kinase signalling pathway in the course of herbimycin A-induced K562 cells differentiation.