RESUMO
Elevation in the plasma levels of the acute phase proteins--C-reactive protein (C-RP) and fibrinogen--were found after injection of leukocytic endogenous mediator (LEM) into rabbits. C-RP in the plasma was elevated 8 hr after injection of LEM, and maximum elevation occurred 24 hr after injection. Injection of LEM into rabbits also produced alterations in body temperature, in levels of plasma iron and zinc, and in the number of neutrophils in the peripheral blood.
Assuntos
Proteína C-Reativa/biossíntese , Fibrinogênio/análise , Animais , Líquido Ascítico/citologia , Proteína C-Reativa/análise , Febre , Granulócitos/análise , Injeções Intraperitoneais , Ferro/sangue , Leucócitos/fisiologia , Peso Molecular , Coelhos , Zinco/sangueRESUMO
The release of endogenous pyrogen (EP) from rabbit peritoneal granulocytes was measured with a three-point log dose-response curve. Release of EP was inhibited when the cells were incubated in media containing potassium or calcium. Measurements of leukocytic endogenous mediator (LEM) activity, i.e., lowering of plasma iron and zinc and increases in blood neutrophils, were made on the same supernatant media. When EP release was inhibited there was a similar inhibition of LEM activity. These results indicate a similarity between the release of pyrogenic and LEM activities. Together with previous purification studies, the results suggest that EP and LEM are similar and may be identical factors.