Assuntos
Transferência Embrionária , Animais , Bovinos , Colo do Útero , Feminino , Métodos , Gravidez , Transplante HomólogoAssuntos
Estro/fisiologia , Luz , Comportamento Sexual Animal/fisiologia , Animais , Feminino , Masculino , MamíferosAssuntos
Inseminação Artificial , Reprodução , Suínos/fisiologia , Animais , Cateterismo , Meio Ambiente , Estro/efeitos dos fármacos , Feminino , Gonadotropinas Equinas/farmacologia , Luz , Masculino , Métodos , Ovulação/efeitos dos fármacos , Gravidez , Progestinas/farmacologia , Propilaminas/farmacologia , Estações do Ano , Sêmen , Semicarbazidas/farmacologia , Espermatozoides , TemperaturaRESUMO
In order to investigate the eventual role of XX/XY chimerism in freemartinism the vascular anastomoses between twin fetuses were surgically suppressed in litters with several fetuses, before the appearance of the first sexual anomalies. In three female fetuses isolated from their co-twins on days 37 and 45, the initial freemartin effect of gonadal and Müllerian inhibition was absent, in spite of an important XX/XY chimerism in the liver.
Assuntos
Quimera , Freemartinismo/embriologia , Mosaicismo , Ductos Paramesonéfricos/fisiologia , Ovário/embriologia , Cromossomos Sexuais , Animais , Bovinos , Feminino , Freemartinismo/genética , Fígado/ultraestruturaRESUMO
Monoclonal antibodies against bovine anti-Müllerian hormone (AMH) were used to study the hormone in cattle. Anti-Müllerian activity of testicular tissue, immunoreactive testicular AMH, serum AMH concentration and AMH production by incubated testicular tissue were detectable from 42 days, i.e. at the time of seminiferous tubule differentiation, and peaked between 50 and 80 days, when the Müllerian ducts regress in the male fetus. All the values stabilized at a lower level until 30 days after birth and then slowly decreased. At 18 months, only traces of AMH immunoreactivity were detectable in testicular tissue and serum concentration and AMH production by incubated testicular tissue were negligible; the main source of AMH in the adult animal was the rete testis fluid. Study of the disappearance rate of AMH from the serum of castrated calves gave a half-life of approximately 2 days for bovine AMH.
Assuntos
Glicoproteínas , Inibidores do Crescimento , Hormônios Testiculares/fisiologia , Envelhecimento , Animais , Animais Recém-Nascidos , Hormônio Antimülleriano , Anticorpos Monoclonais , Castração , Bovinos , Feminino , Idade Gestacional , Técnicas Imunoenzimáticas , Masculino , Rede do Testículo/metabolismo , Células de Sertoli/metabolismo , Hormônios Testiculares/biossíntese , Hormônios Testiculares/sangue , Testículo/embriologia , Testículo/metabolismoRESUMO
Several parameters for serological tests on boar spermatozoa were studied, and a reliable technique was developed and employed. Using SLA (the MHC system in pig) genotyped boars, and specific reagents, SLA and Ia-like antigens were demonstrated on the sperm using both the cytotoxic and the absorption test. No SLA activity was detected in the seminal fluid.