Role of innate immunity and altered intestinal motility in LPS- and MnCl2-induced intestinal intussusception in mice.

Intestinal intussusception (ISS) commonly causes intestinal obstruction in children. One mechanism that has been proposed to cause ISS is inflammation-induced alteration of intestinal motility. We investigated whether innate inflammatory factors or altered motility is required for induction of ISS by LPS. We compared rates of ISS among BALB/c and C57BL/6 mice, mice lacking lymphocytes or depleted of phagocytes, or mice with defects in the Toll-like receptor 4 (TLR4) signaling pathway following administration of LPS or the Ca(2+) analog MnCl2. At 6 or 2 h after administration of LPS or MnCl2, respectively, mice underwent image analysis to assess intestinal contraction rate or laparotomy to identify ISS. LPS-induced ISS (LPS-ISS) was observed in BALB/c mice, but not in C57BL/6 mice or any BALB/c mice with disruptions of TLR4 signaling. LPS-induced serum TNF-α, IL-6, and nitric oxide (NO) and intestinal NO levels were similar in BALB/c and C57BL/6 mice. The rate of LPS-ISS was significantly reduced in phagocyte-depleted, but not lymphocyte-deficient, mice. Intestinal contraction rates were reduced in LPS-ISS-susceptible BALB/c mice, but not in LPS-ISS-resistant C57BL/6 or TLR4 mutant mice, suggesting a role for reduced intestinal contraction rate in LPS-ISS susceptibility. This was tested with MnCl2, a Ca(2+) antagonist that reduced intestinal contraction rates and induced ISS, irrespective of mouse strain. Therefore, LPS-ISS is initiated by innate immune signaling that requires TLR4 and phagocytes but may be independent of TNF-α, IL-6, and NO levels. Furthermore, alteration of intestinal motility, specifically, reduced intestinal contraction rate, is a key factor in the development of ISS.

Immune mediators of rotavirus antigenemia clearance in mice.

The immunological mediators that clear rotavirus antigenemia or viremia remain undefined. Immunodeficient mice and antibody transfer were used to test whether lymphocytes or rotavirus-specific serum antibodies are essential for resolving antigenemia. Clearance of antigenemia required lymphocytes, but neither T nor B lymphocytes were absolutely required. Transfer of convalescent-phase or nonneutralizing rotavirus-specific serum antibodies to the systemic compartment of severe-combined-immunodeficient (SCID) mice temporarily suppressed the onset or level of chronic rotavirus antigenemia. Our findings provide the first report demonstrating that clearance of rotavirus antigenemia and possibly viremia are mediated by multiple effector lymphocyte subsets and serum antibodies.

Mesospheric Bore Evolution and Instability Dynamics Observed in PMC Turbo Imaging and Rayleigh Lidar Profiling Over Northeastern Canada on 13 July 2018.

Two successive mesospheric bores were observed over northeastern Canada on 13 July 2018 in high-resolution imaging and Rayleigh lidar profiling of polar mesospheric clouds (PMCs) performed aboard the PMC Turbo long-duration balloon experiment. Four wide field-of-view cameras spanning an area of ~75 × 150 km at PMC altitudes captured the two evolutions occurring over ~2 hr and resolved bore and associated instability features as small as ~100 m. The Rayleigh lidar provided PMC backscatter profiling that revealed vertical displacements, evolving brightness distributions, evidence of instability character and depths, and insights into bore formation, ducting, and dissipation. Both bores exhibited variable structure along their phases, suggesting variable gravity wave (GW) source and bore propagation conditions. Both bores also exhibited small-scale instability dynamics at their leading and trailing edges. Those at the leading edges comprised apparent Kelvin-Helmholtz instabilities that were advected downward and rearward beneath the bore descending phases extending into an apparently intensified shear layer. Instabilities at the trailing edges exhibited alignments approximately orthogonal to the bore phases that resembled those seen to accompany GW breaking or intrusions arising in high-resolution modeling of GW instability dynamics. Collectively, PMC Turbo bore imaging and lidar profiling enabled enhanced definition of bore dynamics relative to what has been possible by previous ground-based observations, and a potential to guide new, three-dimensional modeling of bore dynamics. The observed bore evolutions suggest potentially important roles for bores in the deposition of energy and momentum transported into the mesosphere and to higher altitudes by high-frequency GWs achieving large amplitudes.

Cooperative predation in the social amoebae Dictyostelium discoideum.

The eukaryotic amoeba Dictyostelium discoideum is commonly used to study sociality. The amoebae cooperate during development, exhibiting altruism, cheating, and kin-discrimination, but growth while preying on bacteria has been considered asocial. Here we show that Dictyostelium are cooperative predators. Using mutants that grow poorly on Gram-negative bacteria but grow well on Gram-positive bacteria, we show that growth depends on cell-density and on prey type. We also found synergy, by showing that pairwise mixes of different mutants grow well on live Gram-negative bacteria. Moreover, wild-type amoebae produce diffusible factors that facilitate mutant growth and some mutants exploit the wild type in mixed cultures. Finding cooperative predation in D. discoideum should facilitate studies of this fascinating phenomenon, which has not been amenable to genetic analysis before.

FoxP3+ regulatory T cells are not important for rotavirus clearance or the early antibody response to rotavirus.

Regulatory T cells produce TGF-ß that contributes to IgA induction by intestinal commensal bacteria but their importance in IgA responses to pathogens has not been determined. Immunity against the enteropathogen, rotavirus, is dependent on intestinal IgA, but whether FoxP3(+) regulatory T cells contribute to this IgA is unknown. Infection with rotavirus increased the numbers of intestinal FoxP3(+) regulatory T cells. Depletion of FoxP3(+) regulatory T cells altered leukocyte activation but did not significantly alter rotavirus clearance or specific antibody levels. These data suggest FoxP3(+) regulatory T cells are not critical for the early antibody response to rotavirus infection.