RESUMO
Alcoholic extracts of seven plants belonging to the Solanaceae family were phytochemically screened and evaluated for their cytotoxic activity by Brine Shrimp Test (BST) with Artemia salina larvae, Inhibition of Cell Division Test (ICDT) on sea urchin Loxechinus albus fertilized eggs and inhibition of crown gall tumors on Potato Disk Bioassay (PDB). From Salpichroa diffusa, bioassay-guided chromatographic separation afforded some active fractions from which epi-katonic acid was identified.
Assuntos
Artemia/efeitos dos fármacos , Medicina Tradicional , Extratos Vegetais/toxicidade , Rhizobium/efeitos dos fármacos , Ouriços-do-Mar/efeitos dos fármacos , Solanaceae , Animais , Feminino , Humanos , Larva/efeitos dos fármacos , Dose Letal Mediana , Masculino , Fitoterapia , Tumores de Planta , Solanum tuberosum , América do SulRESUMO
From the isopropyl acetate fraction obtained by partition of the leaves ethanolic extract of Alchornea glandulosa, six phenolic compounds were isolated and identified. General lethality of the extract, fractions and compounds were assayed with Brine Shrimp Test (BST) and with Antifeedant Activity Bioassay (AAB) against neonate larvae of Spodoptera frugiperda. Aqueous fraction and ethyl gallate, one of the major constituents of the polar fraction, have shown the highest toxicity in BST. Ethanolic extract, isopropyl acetate fraction and gallic acid reduced significantly larval growth of S. frugiperda neonates.