RESUMO
Subcutaneous inoculation of mice with Borrelia burgdorferi, the causative agent of Lyme disease, results in established infection and the development of acute arthritis and carditis, hallmarks of human disease. Because conflicting results may originate from the site of subcutaneous inoculation, we addressed the dissemination capacity of spirochetes injected in the shoulder region versus the footpad. Spirochetes inoculated in the footpad disseminated to a lesser extent to distant organs, such as the ear and the heart. This resulted in distinct degrees of joint and cardiac inflammation at the peak of the disease. The differences eventually leveled out. These results suggest that caution must be exercised in the interpretation of results obtained with routes of inoculation that do not closely represent the natural site of infection.
Assuntos
Borrelia burgdorferi/patogenicidade , Doença de Lyme/etiologia , Doença de Lyme/microbiologia , Animais , Anticorpos Antibacterianos/sangue , Sequência de Bases , Borrelia burgdorferi/genética , Borrelia burgdorferi/imunologia , Borrelia burgdorferi/isolamento & purificação , Linfócitos T CD4-Positivos/imunologia , DNA Bacteriano/análise , DNA Bacteriano/genética , Modelos Animais de Doenças , Feminino , Humanos , Inflamação/etiologia , Inflamação/microbiologia , Injeções Subcutâneas , Doença de Lyme/imunologia , Doença de Lyme/patologia , Camundongos , Camundongos Endogâmicos C3H , Miocardite/etiologia , Miocardite/microbiologia , Especificidade de Órgãos , Reação em Cadeia da PolimeraseRESUMO
A Salmonella enterica serovar Typhimurium aroA-deficient delivery system was used to target the immunosuppressive protein Salp15 to antigen-presenting cells. In vitro and in vivo infections with Salp15-containing Salmonella resulted in an impaired CD4(+)-T-cell activation, suggesting that the protein was produced by antigen-presenting cells in a physiologically active form.