[Dynamics of the cell cycle in human endothelial cell culture infected with influenza virus].

Cell cycle in a culture of endothelial cells EAhy 926 infected with influenza virus was investigated. Cytometric analysis of culture, synchronized using contact inhibition, has shown that the exposure to the influenza virus in cells EAhy 926 lengthened S-phase of the cell cycle. This result has been tested and proven on culture EAhy 926 treated with nocodazole. Compared with lung carcinoma cells A549, in which influenza virus provokes the arrest of G0/G1 phase of the cycle, elongation of S-phase of cycle at a similar infection of endothelial culture EAhy 926 indicates that the influenza virus differently affects the dynamics of the cell cycle according to the origin of the infected culture.

[Preliminary results of an open-label observational study evaluating the efficacy and safety of Prolia used in women with postmenopausal osteoporosis].

AIM: To evaluate the efficacy and safety of Denosumab (Prolia), a first-line osteoporosis (OP) medication that is a fully human monoclonal antibody to the receptor activator of nuclear factor xB ligand (RANKL), within an open-label observational study. SUBJECTS AND METHODS: Patients aged 50 years or older with postmenopausal OP, who were treated with Prolia in clinical practice, were examined. The concentrations of the bone resorption (BR) marker of C-terminal telopeptide and other laboratory indicators (total serum calcium, total alkaline phosphatase, and creatinine) were measured following 3 months. Adverse drug reactions were recorded. RESULTS: Three months after initiation of the investigation, there was a significant decrease in the BR marker C-terminal telopeptide (by 89%; p<0.0001). There were rare adverse reactions: hypocalcemia in 3 (5.9%) patients, arthralgias in 2 (3.9%), and eczema in 1 (1.9%). There were neither serious adverse events nor study withdrawal cases. CONCLUSION: The preliminary results of the open-label study of Prolia in postmenopausal OP suggest that the significantly lower BR activity determines the efficacy of this drug and its high safety.

Alterations in cell cycle dynamics in human endothelium cell culture infected with influenza virus.

The cell cycle of endothelium EAhy 926 cell culture infected with influenza virus has been studied. Cytometric analysis of cell culture synchronized by contact inhibition revealed the elongation of the S phase of the cell cycle in EAhy 926 cells under the influence of influenza virus. This result was shown in an EAhy 926 culture infected with influenza virus and treated with nocodazole. Comparison of a lung carcinoma A549 cell line in which influenza virus causes G0/G1 arrest and of an endothelial EAhy 926 cell line in which the same infection leads to S-phase elongation allows it to be suggested that different effects of influenza virus on cell cycle dynamics depend on the origin of infected cells.

[Assessment of human mental performance through registration of working gain of quasi-stable brain potential]. / Opredelenie umstvennoi rabotosposobnosti cheloveka putem registratsii rabochego prirashcheniia kvaziustoichivogo potentsiala golovnogo mozga.

A novel method of assessment of mental performance in man (MP) is proposed. It is based on relationships between MP, energetic processes in deep cerebral structures and relevant values of quasi-stable brain potential (QSBP). The measurements can be made by persons without special training having simple equipment. MP is assessed by working gain of QSBP after intersive brain work for 0.5-1 hour. If the gain is above 20 mV, MP in children over 10 years of age and adults is considered high, 20 to 10 mV--as moderate, 10 to 0--as satisfactory, under 0--as unsatisfactory. The method has been successfully tested in practice and has certification.