RESUMO
Phosphoenolpyruvate carboxylase catalyses the primary assimilation of CO(2) in Crassulacean acid metabolism plants. It is activated by phosphorylation, and this plays a major role in setting the day-night pattern of metabolism in these plants. The key factor that controls the phosphorylation state of phosphoenolpyruvate carboxylase is the activity of phosphoenolpyruvate carboxylase kinase. Recent work on Crassulacean acid metabolism plants has established this enzyme as a novel protein kinase and has provided new insights into the regulation of protein phosphorylation. Phosphoenolpyruvate carboxylase kinase is controlled by synthesis and degradation in response to a circadian oscillator. The circadian control of phosphoenolpyruvate carboxylase kinase can be overridden by changes in metabolite levels. The primary effect of the circadian oscillator in this system may be at the level of the tonoplast, and changes in kinase expression may be secondary to circadian changes in the concentration of a metabolite, perhaps cytosolic malate.
Assuntos
Fosfoenolpiruvato Carboxilase/metabolismo , Plantas/enzimologia , Ritmo Circadiano , TemperaturaRESUMO
Homogeneous rat liver ATP-citrate lyase (EC 4.1.3.8) was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase. In agreement with other workers, the maximum level of phosphorylation that we observed was approx. 2 mol/mol of tetramer. Phosphorylated and non-phosphorylated forms of ATP-citrate lyase were prepared. Their kinetic properties were examined using an assay system in which the concentrations of Mg.ATP, magnesium.citrate and CoA were varied systematically at a constant concentration of Mg2+. The phosphorylated form had a two-fold higher Km for Mg.ATP than did the non-phosphorylated form, but no other kinetic differences between the two forms were detected. When ATP-citrate lyase was assayed at a concentration of Mg.ATP well below Km, it was found that phosphorylation of the enzyme correlated well with a decrease of approx. 50% in its activity. This is the first demonstration that phosphorylation can affect the activity of ATP-citrate lyase.
Assuntos
ATP Citrato (pro-S)-Liase/metabolismo , Fígado/enzimologia , Trifosfato de Adenosina/farmacologia , Animais , Cinética , Fosforilação , Proteínas Quinases/metabolismo , Ratos , Fatores de TempoRESUMO
Escherichia coli isocitrate lyase was inactivated by diethylpyrocarbonate in a pseudo-first-order process. The enzyme was completely inactivated by modification of a single histidine residue, but slower modification of further residues also occurred. The substrate, isocitrate, and products, glyoxylate and succinate, protected against inactivation by diethylpyrocarbonate but this was not simply due to binding at the active site. Treatment of the inactivated enzyme with hydroxylamine led to only partial recovery of activity. Diethylpyrocarbonate also reacted with sulphydryl groups in isocitrate lyase, as judged by titrations with Nbs2, but this reaction was not responsible for the failure of hydroxylamine to reactivate the enzyme fully. The reactivity of isocitrate lyase to diethylpyrocarbonate declined with pH, following a titration curve for a group of pKa 6.1. Isolation and sequencing of ethoxyformylated peptides showed that the major site of modification by diethylpyrocarbonate was histidine residue 306.
Assuntos
Dietil Pirocarbonato/farmacologia , Escherichia coli/enzimologia , Histidina , Isocitrato Liase/antagonistas & inibidores , Sequência de Aminoácidos , Sítios de Ligação , Reativadores Enzimáticos/farmacologia , Concentração de Íons de Hidrogênio , Hidroxilamina , Hidroxilaminas/farmacologia , Isocitrato Liase/química , Isocitratos/antagonistas & inibidores , Isocitratos/metabolismo , Cinética , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Compostos de Sulfidrila , TripsinaRESUMO
Illumination of maize leaves increases the phosphorylation state of phosphoenolpyruvate carboxylase and reduces the sensitivity of the enzyme to feedback inhibition by malate. Red, white and blue light were each found to be equally potent, and the effect of light was blocked by 3(3,4-dichlorophenyl)-1,1-dimethylurea. A phosphoenolpyruvate carboxylase kinase was partially purified from illuminated maize leaves by a three-step procedure. Phosphorylation of phosphoenolpyruvate carboxylase by this protein kinase reached 0.7-0.8 molecules/subunit and correlated with a 3- to 4-fold increase in Ki for malate. The protein kinase was inhibited by L-malate, but was insensitive to a number of other potential regulators. Freshly prepared and desalted extracts of darkened maize leaves contained very little kinase activity, but the activity appeared when leaves were illuminated for 30-60 min before extraction. The catalytic subunit of protein phosphatase 2A from rabbit skeletal muscle, but not that of protein phosphatase 1, could dephosphorylate phosphoenolpyruvate carboxylase. The protein phosphatases 1 and 2A activities of maize leaves were not affected by illumination. It is suggested that the major means by which light stimulates the phosphorylation of phosphoenolpyruvate carboxylase is by an increase in the activity of the protein kinase.
Assuntos
Luz , Fosfoenolpiruvato Carboxilase/metabolismo , Proteínas Quinases/efeitos da radiação , Zea mays/metabolismo , Cromatografia , Ativação Enzimática/efeitos da radiação , Cinética , Malatos/metabolismo , Fosforilação , Proteínas Quinases/isolamento & purificação , Proteínas Quinases/metabolismo , Especificidade por Substrato , Zea mays/enzimologia , Zea mays/efeitos da radiaçãoRESUMO
Two different crystal forms of isocitrate lyase (ICL) from Escherichia coli have been grown following the chemical modification of the enzyme by either 3-bromopyruvate or ethyl mercuri thiosalicylate (EMTS), contrasting strongly with difficulties in obtaining ordered crystals of the native enzyme. Both crystal forms are obtained using the hanging drop method of vapour diffusion with ammonium sulphate as the precipitant. The crystals diffract well and X-ray photographs of the crystals have established that they are in space groups C222(1) and P3(1) (or its enantiomorph P3(2), respectively. Considerations of the values of Vm and measurements on the crystal density indicate that the asymmetric unit of both crystals contains four subunits.
Assuntos
Escherichia coli/enzimologia , Isocitrato Liase/química , Piruvatos/farmacologia , Timerosal/farmacologia , Cristalização , Isocitrato Liase/isolamento & purificação , Substâncias Macromoleculares , Conformação Proteica , Difração de Raios XRESUMO
The role of NAD-malic enzyme (NAD-ME) in the Crassulacean acid metabolism plant Bryophyllum (Kalanchoe) fedtschenkoi was investigated using preparations of intact and solubilized mitochondria from fully expanded leaves. Intact, coupled mitochondria isolated during the day or night did not differ in their ability to take up [14C]malic acid from the surrounding medium or to respire using malate or succinate as substrate. However, intact mitochondria isolated from plants during the day decarboxylated added malate to pyruvate significantly faster than mitochondria isolated from plants at night. NAD-ME activity in solubilized mitochondrial extracts showed hysteretic kinetics and was stimulated by a number of activators, including acetyl-coenzyme A, fructose-1,6-bisphosphate, and sulfate ions. In the absence of these effectors, reaction progress curves were nonlinear, with a pronounced acceleration phase. The lag period before a steady-state rate was reached in assays of mitochondrial extracts decreased during the photoperiod and increased slowly during the period of darkness. However, these changes in the kinetic properties of the enzyme could not account for the changes in the rate of decarboxylation of malate by intact mitochondria. Gel-filtration experiments showed that mitochondrial extracts contained three forms of NAD-ME with different molecular weights. The relative proportions of the three forms varied somewhat throughout the light/dark cycle, but this did not account for the changes in the kinetics behavior of the enzyme during the diurnal cycle.
RESUMO
Ultraviolet irradiation of E.coli isocitrate dehydrogenase kinase/phosphatase in the presence of 8-azidoATP resulted in parallel losses of its kinase and phosphatase activities, and in covalent attachment of the reagent to the protein at a single site. ATP and ADP protected the two activities to similar extents. The data suggest that the activation of the phosphatase by adenine nucleotides results from binding of the nucleotides to the active site of the kinase.
Assuntos
Trifosfato de Adenosina/metabolismo , Proteínas de Bactérias/metabolismo , Escherichia coli/enzimologia , Fosfoproteínas Fosfatases/metabolismo , Proteínas Quinases/metabolismo , Proteínas Serina-Treonina Quinases , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/análogos & derivados , Marcadores de Afinidade , Azidas , Sítios de LigaçãoRESUMO
Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the sigmoid component of the inhibition by fructose 2,6-bisphosphate; this compound is a simple linear competitive inhibitor of the modified enzyme. The results suggest that fructose 2,6-bisphosphate can bind to the enzyme at two distinct sites, the catalytic site and an allosteric site. High levels of fructose 1,6-bisphosphate probably inhibit by binding to the allosteric site.
Assuntos
Frutose-Bifosfatase/metabolismo , Frutosedifosfatos/metabolismo , Hexosedifosfatos/metabolismo , Fígado/enzimologia , Regulação Alostérica , Sítio Alostérico , Animais , Etilmaleimida/farmacologia , Cinética , RatosRESUMO
NADP+ can protect active isocitrate dehydrogenase against attack by several proteases. Inactive phosphorylated isocitrate dehydrogenase is much less susceptible to proteolysis than the active enzyme, and it is not protected by NADP+. The results suggest that binding of NADP+ to, or phosphorylation of, active isocitrate dehydrogenase induces similar conformational states. Fluorescence titration experiments show that NADPH can bind to active but not to inactive isocitrate dehydrogenase. It is suggested that the phosphorylation of isocitrate dehydrogenase may occur close to its coenzyme binding site.
Assuntos
Escherichia coli/enzimologia , Isocitrato Desidrogenase/metabolismo , NADP/metabolismo , Fosfoproteínas/metabolismo , Serina Endopeptidases , Endopeptidases/metabolismo , Ativação Enzimática , NADP/farmacologia , Peptídeo Hidrolases/metabolismo , Fosforilação , Espectrometria de FluorescênciaRESUMO
Isocitrate dehydrogenase from Escherichia coli is regulated by a reversible phosphorylation mechanism. We report here the amino acid sequence round the phosphorylation site; this is the first such sequence to be reported for a bacterial protein kinase. The sequence does not resemble sequences phosphorylated by cyclic AMP-dependent protein kinase.
Assuntos
Escherichia coli/enzimologia , Isocitrato Desidrogenase/metabolismo , Sequência de Aminoácidos , Quimotripsina , Cinética , Fragmentos de Peptídeos/análise , Fosfopeptídeos/análise , Fosforilação , TermolisinaRESUMO
One of the important effects of insulin on intracellular metabolism is its ability to stimulate the synthesis of glycogen in muscle and liver. It does this by promoting a net decrease in the extent of phosphorylation of glycogen synthase, the rate-limiting enzyme in the pathway of glycogen synthesis, which increases its activity. Several years ago glycogen synthase was shown to be phosphorylated and inactivated by cyclic AMP-dependent protein kinase in vitro, suggesting that the effect of insulin on glycogen synthesis, and perhaps other intracellular processes, might be explainable in terms of the ability of the hormone to decrease the concentration of tissue cyclic AMP. However, the subsequent failure to detect a decrease in cyclic AMP concentration in muscle under conditions where glycogen synthase activity was stimulated by insulin, coupled with the discovery of a second glycogen synthase kinase whose activity is unaffected by cyclic nucleotides, now suggests the possibility that insulin may regulate the activity of a different class of protein kinase, through its own "second messenger". The identification and characterization of glycogen synthase kinase-2 and recent information about the regulation of glycogen synthase by phosphorylation-dephosphorylation in vitro and in vivo are presented.
Assuntos
Glicogênio/biossíntese , Insulina/fisiologia , Sequência de Aminoácidos , Animais , Glicemia , AMP Cíclico/metabolismo , Ativação Enzimática , Epinefrina/fisiologia , Glicogênio Sintase/metabolismo , Glicogênio Sintase-D Fosfatase/metabolismo , Humanos , Insulina/farmacologia , Glicogênio Hepático/biossíntese , Camundongos , Músculos/metabolismo , Proteínas Quinases/metabolismo , Receptor de Insulina/metabolismoRESUMO
During growth of Escherichia coli on acetate, the glyoxylate bypass supplies the precursors needed for biosynthesis. The glyoxylate bypass enzyme isocitrate lyase competes with the citric acid cycle enzyme isocitrate dehydrogenase for the available isocitrate. We have studied the control of metabolic flux at this branchpoint by examining the regulatory properties of the enzymes concerned. Isocitrate dehydrogenase is controlled by reversible phosphorylation catalysed by a bifunctional kinase/phosphatase whose activities are regulated by isocitrate, biosynthetic precursors and adenine nucleotides. The flux through isocitrate lyase is mainly controlled by the intracellular concentration of isocitrate. The phosphorylation system responds to the availability of energy and precursors and maintains isocitrate at a concentration high enough to sustain the flux through the glyoxylate bypass necessary for biosynthesis.
Assuntos
Ciclo do Ácido Cítrico , Escherichia coli/enzimologia , Glioxilatos/metabolismo , Isocitrato Desidrogenase/metabolismo , Isocitrato Liase/metabolismoRESUMO
Escherichia coli isocitrate dehydrogenase is completely inactivated by phosphorylation of a single serine residue per subunit. We have examined the conformations of the active and phosphorylated forms of the enzyme using circular dichroism spectroscopy. The results support the view that phosphorylation prevents the binding of NADP, probably by direct blocking of the coenzyme-binding site. Labelling studies suggest that an arginine residue at the coenzyme-binding site may be close to the phosphorylatable serine residue. The phosphorylation of isocitrate dehydrogenase is thus unusual in that it occurs at the active site of the enzyme. We therefore investigated the recognition of isocitrate dehydrogenase by isocitrate dehydrogenase kinase/phosphatase. The kinase activity of this enzyme can phosphorylate intact isocitrate dehydrogenase but not proteolytic fragments derived from it, nor a synthetic peptide corresponding to the sequence round the phosphorylation site.
Assuntos
Coenzimas/metabolismo , Escherichia coli/enzimologia , Isocitrato Desidrogenase/metabolismo , Monoéster Fosfórico Hidrolases/metabolismo , Arginina/análise , Dicroísmo Circular , NADP/metabolismo , Fosforilação , Conformação Proteica , Desnaturação Proteica , Relação Estrutura-AtividadeRESUMO
There is now good evidence that the malate sensitivity of PEPc is regulated by phosphorylation/dephosphorylation in the leaf tissue of C4 and CAM plants. This statement is based on the assessment of the phosphorylation state of PEPc in [32P]-labeled intact tissue by immunoprecipitation and the correlation between phosphorylation state and malate sensitivity that has been observed during incubation of purified PEPc in vitro with protein kinases or protein phosphatases. The phosphorylation of PEPc in the CAM plant B. fedtschenkoi is controlled by an endogenous rhythm whereas that of PEPc in the C4 plant maize is triggered directly by light. In neither case has the mechanism of signal transduction been identified. It is hoped that further work on the protein kinases and protein phosphatases involved will reveal the nature of the signalling systems. Preliminary work suggests that plant protein phosphatases are very similar to their mammalian counterparts. It is also noteworthy that higher plant genes very similar to the genes encoding the cyclic nucleotide-dependent protein kinases and the protein kinase C family have recently been identified. It is interesting to speculate that the protein kinases and phosphatases involved in signal transduction systems in plants may prove to be closely related to well-studied mammalian enzymes.
Assuntos
Carboxiliases/metabolismo , Fosfoenolpiruvato Carboxilase/metabolismo , Plantas/enzimologia , Transdução de Sinais , Ritmo Circadiano , Cinética , Fosfoproteínas Fosfatases/metabolismo , Radioisótopos de Fósforo , Fosforilação , Proteínas Quinases/metabolismoAssuntos
Glicogênio Sintase , Músculos/enzimologia , Proteínas Quinases/metabolismo , Trifosfato de Adenosina , Animais , Cromatografia DEAE-Celulose , AMP Cíclico/farmacologia , Eletroforese em Gel de Poliacrilamida , Guanosina Trifosfato , Cinética , Peso Molecular , Proteínas Musculares/farmacologia , Radioisótopos de Fósforo , Fosforilases , Inibidores de Proteínas Quinases , Proteínas Quinases/isolamento & purificação , Coelhos , Fatores de Tempo , TripsinaAssuntos
Ritmo Circadiano/fisiologia , Plantas/enzimologia , Proteínas Quinases/metabolismo , Proteínas Serina-Treonina Quinases/biossíntese , Aclimatação , Dióxido de Carbono/metabolismo , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Fosfoenolpiruvato Carboxilase/metabolismo , Fenômenos Fisiológicos VegetaisRESUMO
Of the many plant genes whose expressions are controlled by the circadian clock, one of the phosphoenolpyruvate carboxylase kinase genes in soya bean (Glycine max) exhibits the unusual property that its control is organ-specific--it is under circadian control in leaves but not in roots. Preliminary experiments suggest that the same is true for at least one gene in Arabidopsis thaliana. It will be important to define the extent and function of this phenomenon and the underlying mechanism.