[The role of PD-ECGF and VEGF in proliferative and involuted mechanism of the infantile capillary hemangiomas].

OBJECTIVE: To explore the relation between the expressions of PD-ECGF and VEGF and the evolution of capillary hemangioma, so as to provide theoretical basis for treatment. METHODS: Fourty cases with capillary hemangioma, proved by pathologic method, were randomly selected and divided into proliferative (n=22) and involuted groups (n=18), according to the Mulliken standard. 8 specimens from 8 children with prepuce operation were used as control group. All the specimens were fixed, embedded and underwent HE staining. The expression of PD-ECGF, VEGF and CD34 in endothelial cells were detected by immunohistochemistry. The microvessel-density (MVD) was also calculated. The results were analyzed by SPSS12.0. RESULTS: The positive expression rates of PD-ECGF and VEGF were 95.45% (21/22) and 86.36% (19/22) in proliferative hemangioma, 77.78% (14/18) and 66.67% (12/ 18) in involuted hemangioma, 37.50% (3/8) and 37.50% (3/8) in normal skin. MVD in proliferative and involuted hemangioma and normal skin was 93.68 +/- 20.56, 51.94 +/- 20.73 and 17.50 +/- 5.30, respectively. There was a significant difference in PD-ECGF expression and MVD between the proliferative and involuted groups, or between the hemangioma and control groups (P < 0.05). The VEGF was significantly different between the proliferative and involuted groups, or between the proliferative and control groups (P < 0.05), but not between the involuted and control groups (P > 0.05). The expression of VEGF, PD-ECGD and MVD showed a positive relationship. CONCLUSIONS: PD-ECGF and VEGF have a synergetic effect in the proliferation of micro-vessels. PD-ECGF may enhance the activity of thymidine phosphorylase. They play an important role in the proliferation and involution of hemangioma.

Role of color Doppler flow imaging in applicable anatomy of spleen vessels.

AIM: To explore the role of color Doppler flow imaging (CDFI) in visualization of spleen vessels and to define its value for spleen micro-invasive operation. METHODS: A total of 36 patients requiring laparoscopic splenectomy (LS) for various hematopathies and autoimmune diseases were randomly selected from April 2005 to May 2008. Anatomic types of spleen pedicle, adjacent relations between spleen vessels and pancreas, diameters of spleen artery and vein were detected and recorded by preoperative CDFI. Different operative procedures were performed according to different anatomic frameworks. The parameters were recorded by telerecording during LS and compared with those by preoperative CDFI using Chi-square test. RESULTS: Two anatomic types of spleen pedicle and four different adjacent relations between spleen vessels and pancreas were detected by CDFI. The diameters of spleen artery and vein detected by CDFI were 0.46 +/- 0.09 cm and 0.85 +/- 0.35 cm, respectively. There was no statistical difference between the parameters recorded by CDFI and by telerecording (chi2 = 0.250, 0.677, P > 0.05). LS was successfully performed following the anatomic information provided by preoperative CDFI. CONCLUSION: Different anatomic frameworks of spleen vessels can be provided by preoperative CDFI, which instructs micro-invasive operation of spleen and increase the safety of operation.

[Pingyangmycin emulsion inducing apoptosis in infantile proliferating capillary hemangiomas].

OBJECTIVE: To insight whether PYM emulsion induces apoptosis more rapidly in proliferating capillary hemangiomas so as to find the optimal method for hemangiomas' treatment. METHODS: Thirty volunteers of infantile proliferating hemangiomas were divided into control group (15 cases) and mediational group (15 cases). PYM was made into emulsion and smeared on the surfaces of the lesion in mediational group with 3 times every day as well as only matrix in control group. The specimens were resected on day 7, then made into pathological slices and electron microscope slices in order to observe the cells microcosmic structure changes and ultrastructure changes. Furthermore , the apoptotic index of two groups were detected by the molecular biology method (TUNEL test ). RESULTS: The number of apoptotic cells were lower in control group (AI 9.693 +/- 4.948) but higher apparently in mediational group (AI 39.373 +/- 15.927). The difference between two groups was significant (t = 6.893, P < 0.01) . CONCLUSIONS: PYM emulsion can effectively accelerate apoptosis in infantile proliferating capillary hemangiomas . The mechanism is supposed to related to the blockage of cell cycle and activation of apoptotic signal transduction pathway.