A mitochondrial RNA processing protein mediates plant immunity to a broad spectrum of pathogens by modulating the mitochondrial oxidative burst.

Mitochondrial function depends on the RNA processing of mitochondrial gene transcripts by nucleus-encoded proteins. This posttranscriptional processing involves the large group of nuclear-encoded pentatricopeptide repeat (PPR) proteins. Mitochondrial processes represent a crucial part in animal immunity, but whether mitochondria play similar roles in plants remains unclear. Here, we report the identification of RESISTANCE TO PHYTOPHTHORA PARASITICA 7 (AtRTP7), a P-type PPR protein, in Arabidopsis thaliana and its conserved function in immunity to diverse pathogens across distantly related plant species. RTP7 affects the levels of mitochondrial reactive oxygen species (mROS) by participating in RNA splicing of nad7, which encodes a critical subunit of the mitochondrial respiratory chain Complex I, the largest of the four major components of the mitochondrial oxidative phosphorylation system. The enhanced resistance of rtp7 plants to Phytophthora parasitica is dependent on an elevated mROS burst, but might be independent from the ROS burst associated with plasma membrane-localized NADPH oxidases. Our study reveals the immune function of RTP7 and the defective processing of Complex I subunits in rtp7 plants resulted in enhanced resistance to both biotrophic and necrotrophic pathogens without affecting overall plant development.

Influence of rapid vertical mixing on bacterial community assembly in stratified water columns.

Water column mixing homogenizes thermal and chemical gradients which are known to define distribution of microbial communities and influence the prevailing biogeochemical processes. Little is however known about the effects of rapid water column mixing on the vertical distribution of microbial communities in stratified reservoirs. To address this knowledge gap, physicochemical properties and microbial community composition from 16 S rRNA amplicon sequencing were analyzed before and after mixing of vertically stratified water-column bioreactors. Our results showed that α-diversity of bacterial communities decreased from bottom to surface during periods of thermal stratification. After an experimental mixing event, bacterial community diversity experienced a significant decrease throughout the water column and network connectivity was disrupted, followed by slow recovery. Significant differences in composition were seen for both total (DNA) and active (RNA) bacterial communities when comparing surface and bottom layer during periods of stratification, and when comparing samples collected before mixing and after re-stratification. The dominant predicted community assembly processes for stratified conditions were deterministic while such processes were less important during recovery from episodic mixing. Water quality characteristics of stratified water were significantly correlated with bacterial community diversity and structure. Furthermore, structural equation modeling analyses showed that changes in sulfur may have the greatest direct effect on bacterial community composition. Our results imply that rapid vertical mixing caused by episodic weather extremes and hydrological operations may have a long-term effect on microbial communities and biogeochemical processes.

Microbial community structure and denitrification responses to cascade low-head dams and their contribution to eutrophication in urban rivers.

Low-head dams are one of the most common hydraulic facilities, yet they often fragment rivers, leading to profound changes in aquatic biodiversity and river eutrophication levels. Systematic assessments of river ecosystem structure and functions, and their contribution to eutrophication, are however lacking, especially for urban rivers where low-head dams prevail. In this study, we address this gap with a field survey on microbial community structure and ecosystem function, in combination with hydrological, environmental and ecological factors. Our findings revealed that microbial communities showed significant differences among the cascade impoundments, which may be due to the environment heterogeneity resulting from the cascade low-head dams. The alternating lentic-lotic flow environment created by the low-head dams caused nutrient accumulation in the cascade impoundments, enhancing environmental sorting and interspecific competition relationships, and thus possibly contributing to the reduction in sediment denitrification function. Decreased denitrification led to excessive accumulation of nutrients, which may have aggravated river eutrophication. In addition, structural equation model analysis showed that flow velocity may be the key controlling factor for river eutrophication. Therefore, in the construction of river flood control and water storage systems, the location, type and water storage capacity of low-head dams should be fully considered to optimize the hydrodynamic conditions of rivers. To summarize, our findings revealed the cumulative effects of cascade low-head dams in an urban river, and provided new insights into the trade-off between construction and decommissioning of low-head dams in urban river systems.

Responses of bacterial community and N-cycling functions stability to different wetting-drying alternation frequencies in a riparian zone.

Wetting-drying alternation (WD) of the soil is one of the key characteristics of riparian zones shaped by dam construction, profoundly impacting the soil microenvironment that determines the bacterial community. Knowledge concerning the stability of bacterial community and N-cycling functions in response to different frequencies of WD remains unclear. In this study, samples were taken from a riparian zone in the Three Gorges Reservoir (TGR) and an incubation experiment was conducted including four treatments: constant flooding (W), varied wetting-drying alternation frequencies (WD1 and WD2), and constant drying (D) (simulating water level of 145 m, 155 m, 165 m, and 175 m in the riparian zone respectively). The results revealed that there was no significant difference in the diversity among the four treatments. Following the WD1 and WD2 treatments, the relative abundances of Proteobacteria increased, while those of Chloroflexi and Acidobacteriota decreased compared to the W treatment. However, the stability of bacterial community was not affected by WD. Relative to the W treatment, the stability of N-cycling functions estimated by resistance, which refers to the ability of functional genes to adapt to changes in the environment, decreased following the WD1 treatment, but showed no significant change following the WD2 treatment. Random forest analysis showed that the resistances of the nirS and hzo genes were core contributors to the stability of N-cycling functions. This study provides a new perspective for investigating the impacts of wetting-drying alternation on soil microbes.

Diversity and potential functional characteristics of phage communities colonizing microplastic biofilms.

The multiple ecological influences and potential microbial degradation of microplastics are generally attributed to the microbial communities colonized on microplastics. Phages play an important role in the composition and function of their bacterial hosts, yet the occurrence and the potential functional characteristics of phages in the biofilms of microplastics have not been known. This study, for the first time, explored the diversity, composition, and potential function characteristics of phage communities living in the biofilms of PP, PE, and PET microplastics and stones, cultured in the same site, via the metagenome method. The results showed that a total of 240 non-redundant virus OTUs (vOTUs), distributed in at least four orders and seven families, were detected from biofilm metagenomes of microplastics. Compared to stones, some phages were selectively enriched by microplastic biofilms, with 13 vOTUs uniquely colonized on three microplastics, and these vOTUs mainly belong to the family Autographiviridae and Podoviridae. Except for the evenness of PP, the richness index, Chao 1 index, and abundance of phage communities of three microplastics were much higher than that of stone. At least 8 bacterial phyla and 72 genera were possibly infected by phages. Compared to the stones, both composition and abundance of the phages and hosts presented significant and strong correlations for three microplastics. Some of the bacterial hosts on microplastics were likely involved in the microplastic degradation, fermenters, nitrogen transformation processes, and so on. A total of 124 encoding auxiliary metabolic genes (AMGs) were detected from viral contigs. The abundance of AMGs in microplastics was much higher than that of stones, which may provide more direct or indirect support for the bacterial degradation of microplastics. This study provides a new perspective on the occurrence and potential functions of phages on microplastic biofilms, thus expanding our understanding of microbial communities on microplastic biofilms.

Trophic interactions regulate microbial responses to environmental conditions and partially counteract nitrogen transformation potential in urban river bends.

River bends are distinguished by high biodiversity and elevated rates of biogeochemical activities due to complex hydromorphological processes that form diverse geomorphic units, making it challenging to elucidate the impact of trophic interactions on community assembly and biogeochemical processes. Here, we clarify the effect of trophic interactions in determining the assembly of multi-trophic microbial communities and the impact on nitrogen transformation potential by distinguishing the direct and cascading effects of environmental conditions based on 32 samples collected from a typical urban river bends. It was found that both bacterial and micro-eukaryotic communities were determined by homogeneous selection (indicated by ß-nearest taxon index, accounted for 85% and 48.3%, respectively), whereas the dominant environmental factors were different, being sediment particle size (P < 0.05) and nitrogen (P < 0.05), respectively. Both the microbial co-occurrence network and the significant association (P < 0.05) between ß-nearest taxon index and trophic transfer efficiency changes showed that the trophic interactions strongly shaped microbial communities in the urban river bends. The path modeling suggested that environmental conditions resulted in an increase in abundance of multi-trophic microbial communities via direct effects (mean standardized effects = 0.21), but reductions in abundance of bacteria via cascading effects, i.e., trophic interaction (mean standardized effects = -0.1). When considering direct and cascading effects together, environmental conditions in urban river bends were found to enhance the abundance of microbial communities, with decreasing magnitude at the higher trophic level. Analogously, the path modeling also indicated the nitrogen transformation potential enhanced by environmental conditions via direct effects, but partly counteracted by trophic interactions via cascading effects. The obtained results could provide a theoretical basis for the regulation and restoration of urban rivers.

Enantioselective Total Synthesis of (-)-Cephalotanin B.

Cephalotaxus diterpenoids are attractive natural products with intriguing molecular frameworks and promising biological features. As a structurally unusual member, (-)-cephalotanin B possesses an extraordinarily congested heptacyclic skeleton, three lactone units, and nine consecutive stereocenters. Herein, we report an enantioselective total synthesis of (-)-cephalotanin B based on a divergent asymmetric Michael addition reaction, a novel Pauson-Khand/deacyloxylation process discovered in the development of a second-generation stereoselective Pauson-Khand reaction protocol, and an epoxide-opening/elimination/dual-lactonization cascade to construct the challenging propeller-shaped A-B-C ring system as key transformations.

Dam-induced flow velocity decrease leads to the transition from heterotrophic to autotrophic system through modifying microbial food web dynamics.

The impoundment of reservoirs changes the river from a riverine heterotrophic system to a lacustrine autotrophic system, which could be attributed to the shift of pelagic microbial food webs in response to the dam-induced disturbances. However, little is known about what is the key factor controlling this variation and how different underlying interactions affect the food web dynamics. This study investigated the effects of flow velocity and nutrient supply on microbial plankton using a microcosm experiment. The results showed that flow velocity decrease was the main factor inducing the detritus-based food web transformed to the autotroph-based food web, with heterotrophic bacteria and protozoan dominated at high velocity, whereas phytoplankton and metazoan were prevalent in the lentic environment. The lentic-acclimated genera, such as Chlorella sp., Mallomonas sp. and Microcystis sp., showed hysteresis after the velocity recovery, suggesting the potential of algae bloom in reservoirs and even downstream of dams. We further conducted a flow-velocity manipulating experiment and constructed a multi-trophic nitrogen cycling model to provide a mechanistic explanation for the microbial food web dynamics and the nitrogen transformation performances. As indicated in model prediction and sensitivity analysis, the abiotic and biotic variations were directly or indirectly controlled by nutrient utilization and predator-prey interactions. Quantification of these bottom-up and top-down forces revealed the buffer role of predators in mitigating the positive effects of nutrient availability on autotrophs at low velocity and on heterotrophs at high velocity, respectively. This study highlights the importance of mastering the whole information of different trophic levels, in order to better capture the complex microbial food web interactions and the consequent biogeochemical processes in river-reservoir systems.

Effects of long-term exposure to silver nanoparticles on the structure and function of microplastic biofilms in eutrophic water.

Microplastics are frequently detected in natural aquatic systems proximate to populated areas, such as urban rivers and lakes, and can be rapidly colonized by microbial communities. Microplastics and silver nanoparticles (AgNPs) share similar pathways into natural waters and tend to form heteroaggregations. However, very little is known about the long-term impacts on the structure and function of microplastic biofilms when chronically exposed to silver nanoparticles. Thus, the present study assessed the accumulation property of AgNPs on polymethyl methacrylate (PMMA) microplastics via adsorption tests and studied the chronic effects of AgNPs on the structure and function of microplastic biofilms via 30-day microcosmic experiments in eutrophic water. The adsorption tests showed that the biofilms-colonized PMMA microplastics presented the highest adsorption of 0.98 mg/g in the 1 mg/L AgNPs microcosms. After the 30-day exposure, lactic dehydrogenase release and reactive oxygen species generation of PMMA biofilms increased by 33.23% and 23.98% compared to the MPs-control group with no-AgNPs, indicating that the number of dead cells colonizing microplastics significantly increased. Network analysis suggested that the stabilization of the bacterial community declined with the long-term exposure to AgNPs through the reduction of the modularity and average path length of the network. Compared to the MPs-control group, long-term exposure to AgNPs caused cumulatively inhibitory effects on the nitrogen removal and the N2O emissions in eutrophic water. The isotopomer analysis revealed that the contribution rate of NO2- reduction to N2O emissions was gradually increasing with the AgNPs exposure. Real-time PCR analysis showed that denitrification genes were less sensitive to AgNPs than the nitrification genes, with gene nosZ performed the most negligible response. Overall, our results revealed that long-term exposure to AgNPs could alter biogeochemical cycling involved by microplastic biofilms and cumulatively reduce the self-recovery of the eutrophic ecosystem.

New insights into nitrogen removal potential in urban river by revealing the importance of microbial community succession on suspended particulate matter.

The importance of suspended particulate matter (SPM) in nitrogen removal from aquatic environments has been acknowledged in recent years by recognizing the role of attached microbes. However, the succession of attached microbes on suspended particles and their role in nitrogen removal under specific surface microenvironment are still unknown. In this study, the causation among characteristics of SPM, composition and diversity of particle-attached microbial communities, and abundances of nitrogen-related genes in urban rivers was firstly quantitatively established by combing spectroscopy, 16 S rRNA amplicon sequencing, absolute gene quantification and supervised integrated machine learning. SPM in urban rivers, coated with organic layers, was mainly composed of silt and clay (87.59-96.87%) with D50 (medium particle size) of 8.636-30.130 µm. In terms of material composition of SPM, primary mineral was quartz and the four most abundant elements were O, Si, C, Al. The principal functional groups on SPM were hydroxyl and amide. Furthermore, samples with low, medium and high levels of ammoxidation potential were classified into three groups, among which significant differences of microbial communities were found. Samples were also separated into three groups with low, medium and high levels of denitrification potential and significant differences occurred among groups. The particle size, content of functional groups and concentration of SPM were identified as the most significant factors related with microbial communities, playing an important role in succession of particle-attached microbes. In addition, the path model revealed the significantly positive effect of organic matter and particle size on the microbial communities and potential nitrogen removal. The content of hydroxyl and temperature were identified as the most effective predicting factors for ammoxidation potential and denitrification potential respectively by Random Forests Regression models, which had good predictive performances for potential of ammoxidation (R2 = 0.71) and denitrification (R2 = 0.61). These results provide a basis for quickly assessing the ability of nitrogen removal in urban rivers.

The role of microbial communities on primary producers in aquatic ecosystems: Implications in turbidity stress resistance.

Expanding the stress tolerance and adaptation potential of primary producers is of importance for the restoration and management of aquatic ecosystems. Microorganisms have been reported to mediate improved resistance toward different abiotic stresses of primary producers in terrestrial and marine ecosystems. However, it is not clear about the role of microbial communities in the turbidity resistance of primary producers, when aquatic ecosystems are under turbidity pressure. In this study, key microbes and the action path which enhance turbidity tolerance of primary producers were recognized by mesocosm and various multivariate statistical methods. Remarkable decrease of the biomass of primary producers was found with the increase of turbidity. Significant differences in microbial community under different turbidity pressure were recognized and key microbes which may expand the turbidity tolerance of primary producers were further identified. Rhodobacter and Rhodoferax were selected as key microbes by the investigation of keystone species in the microbial ecological network and significant discriminant taxa under different turbidity stress. The action path for microbial communities to help primary producers cope with turbidity pressure was found through structural equation model, and in which the increase of key microbes may expand the turbidity tolerance of primary producers through enhancing the microbial loop. The results may provide a new insight for aquatic ecosystems to resist turbidity stress, and provide a theoretical basis for the management and restoration of aquatic ecosystems.

Hydrodynamic zones and the influence of microorganisms on nitrogen transformation in the diverging area of branched rivers.

Diverging area is widespread in river networks, and understanding its biogeochemical process characteristics is of great significance to river ecological restoration and environmental quality improvement. Microbial communities affected by hydrodynamics play an important role in biogeochemical processes, but their relationship in diverging area is little known. Here, the composition of microbial community and its feedback to hydrodynamics and nitrogen conversion in the diverging area of river networks were first studied by coupling ecological theory, biogeochemical theory, microbial DNA sequencing and mathematical model of water environment. The results showed that there were five hydrodynamic zones with significant velocity differences in the diverging area, namely low velocity zone, maximum velocity zone, stagnant zone, separation zone, and deflection zone. According to the flow velocity grouping, there were significant differences in the microbial diversity and abundance among low velocity group, maximum velocity group and stagnant group had significant differences (p < 0.05, stress = 0.1207). In the low velocity group, Firmicutes was the dominant phylum which had a highest abundance and may promot the conversion of organic nitrogen into ammonia nitrogen. In the maximum velocity group, Bdellovibrionota was the dominant phylum which had a highest abundance and may promot the conversion of nitrate and nitric oxide to nitrogen. In the stagnant zone, Methylomirabilota was the dominant phylum which had a highest abundance and may promot the conversion of nitrogen into nitrate and ammonium. In addition, dissolved oxygen was the most sensitive environmental factor for shaping microorganisms and nitrogen conversion in the diverging area of the river networks by canonical correlation analysis. The denitrifying bacteria Rhodocyclaceae, was shown to negatively correlated with the flow velocity. This research improves the scientific basis for the study of the ecosystem in river networks, which will guide the construction of river ecological projects.

New insights into identifying sediment phosphorus sources in river-lake coupled system: A framework for optimizing microbial community fingerprints.

Identifying sediment phosphorus sources in river-lake coupled system is a question in developing preferential control strategies for phosphorus. As sediments adsorbed phosphorus and microbes would be transported with changing hydrodynamic, the phosphorus source-specific microbial community fingerprints shed light on determining the major sediment phosphorus sources. However, the identification of microbial community fingerprints is a challenge because both microbial succession and hydrological characteristics of river-lake systems would affect the stability of fingerprints. Therefore, this study provided a framework for optimizing phosphorus source-specific microbial community fingerprints, and attempted to identify the major sources of sediment phosphorus in river-lake coupled ecosystem. Meiliang Lake is one of the highly eutrophic area in Taihu Lake, where the sediments, bacterial communities, and phosphorus had a close relationship. Through analyzing the connectivity of microbes along water continuum, a microbial fingerprints candidate database was constructed. The phosphorus-related bacterial communities were screened and optimized by comparing the difference of predicted results between upstream and downstream, forming the stable microbial community fingerprints which consisted of Bacteroidia, Bacilli, Clostridia, and other species at the class level. SourceTracker results that based on the optimized phosphorus source-specific microbial community fingerprints indicated that the major sediment phosphorus sources to Meiliang Lake were Liangxi River, Wujingang River, and Donghuandi River, with the relative standard deviations ranging from 2.59% to 27.56%. The accuracy of phosphorus source apportionments was further confirmed based on the composite pollution index and hydrodynamic condition. This study put forward suggestions on how to improve the stability of microbial community fingerprints, and would help to improve the understanding of applying microbial source tracking method to identify the sources of abiotic pollution like sediment phosphorus.

Determination of the direct and indirect effects of bend on the urban river ecological heterogeneity.

The ecological heterogeneity created by river bends benefits the diversity of microorganisms, which is vital for the pollutant degradation and overall river health. However, quantitative tools capable of determining the interactions among different trophic levels and species are lacking, and research regarding ecological heterogeneity has been limited to a few species. By integrating the multi-species-based index of biotic integrity (Mt-IBI) and the structure equation model (SEM), an interactions-based prediction modeling framework was established. Based on DNA metabarcoding, a multi-species (i.e., bacteria, protozoans, and metazoans) based index of biotic integrity including 309 candidate metrics was developed. After a three-step screening process, eight core metrics were obtained to assess the ecological heterogeneity, quantitatively. The Mt-IBI value, which ranged from 2.08 to 7.17, was calculated as the sum of each single core metric value. The Mt-IBI revealed that the ecological heterogeneity of concave banks was higher than other sites. According to the result of the SEM, D90 was the controlling factor (r = -0.779) of the ecological heterogeneity under the influence of the river bends. The bend-induced redistribution of sediment particle further influenced the concentrations of carbon, nitrogen, and sulphur. The nitrogen group (r = 0.668) also played an essential role in determining the ecological heterogeneity, follow by carbon group (r = 0.455). Furthermore, the alteration of niches would make a difference on the ecological heterogeneity. This multi-species interactions-based prediction modeling framework proposed a novel method to quantify ecological heterogeneity and provided insight into the enhancement of ecological heterogeneity in river bends.

Depth induced assembly discrepancy of multitrophic microbial communities affect microbial nitrogen transformation processes in river cross-sections.

Understanding how the structures and functions of bacterial and microeukaryotic communities vary within cross-sections will improve managements aimed at restoring river ecological functions. However, no comprehensive investigation has examined how microbial community characteristics vary within cross-sections, which makes the accurate calculation and prediction of microbial metabolic processing of substances in rivers difficult. Here, the distributions, co-occurrence networks, and assemblies of bacterial and microeukaryotic communities and their feedback to nitrogen transformation in cross-sections of the Yangtze River were studied by coupling ecological theory, biogeochemistry, and DNA meta-barcoding methods. The study found that depth in cross-sections was the primary driving factor regulating the composition of sediment bacterial and microeukaryotic communities. Co-occurrence network analysis indicated that the effect of bacteria on the co-occurrence network decreased and the network become more simplified and instability with depth in river cross-sections. Quantified using the ß-nearest taxon index, the H2 layer sediment (depth 10-20 m) displayed the largest variation in selection processes for microbial assemblies, while homogeneous selection and homogenizing dispersal contributed most to the bacterial and microeukaryotic assemblies in the H3 layer (depth >20 m). Cross-sectional depth and denitrification genes had a significant quadratic correlation, with the highest microbial nitrogen-removal potential occurring in the H2 layer sediment. Structural equation models showed that the sediment nitrogen distributions were regulated by distinct environmental pathways at different depths, and that the H2 layer sediment was primary driven by bacterial community. In this layer, river cross-sectional depth influenced nitrogen transformation by regulating the distribution of sediment particle sizes, which then influenced the assembly of the multitrophic microbial communities. This study will improve river management by clarifying the importance of cross-sectional depth to the ecological function of rivers.

Effect of water chemistry on nitrogen transformation, dissolved organic matter composition and microbial community structure in hyporheic zone sediment columns.

Controlled surface water systems, including those with dams lead to dynamic stage changes that alter the fluctuation directions of flow exchange in the hyporheic zones (HZ). However, the nitrogen transformation, dissolved organic matter (DOM) composition, and microbial community responding to variable scenarios of water source and hyporheic exchange are poorly studied. The present work investigated nitrogen transformation in HZ sediments, focusing on how microbial community structure and biological functions related to nitrogen transformation and sediment-attached DOM compositions. Upwelling of synthesized groundwater, downwelling of synthesized river water and exchangeable elution of both feed water created distinct microbial zonation and N-transformation processes. Mixing of river water and groundwater enhanced microbial diversity, microbial co-occurrence network complexity and N-transformation functions. In terms of the sediment-attached DOM properties after hyporheic exchanges, humic fractions occupied the predominant chromophoric DOM. Correlation analysis implied that there were more DOM properties, e.g., tryptophan-like proteins, humic-like fractions, and the source of humic fractions, involved in affecting the microbial community under downwelling flow. Co-occurrence network analysis verified that fluorescent components, protein-like and lignin-like fractions in sediment-detached DOM were clustered with microbial communities in one module in downwelling column, implying closer interactions among microbial communities and DOM fractions. The strains of Nitrospinae, Dinghuibacter, and Lentimicrobium etc. were key species collaborating to metabolize both nitrogen and DOM in HZ sediments. The work provides insights into how the nitrogen transformation, DOM compositional changes, as well as the linkages between community structure and DOM factions, response to the changes in water chemistry, leading to valuable insights into hyporheic zone functions.

Pollution gradients shape the co-occurrence networks and interactions of sedimentary bacterial communities in Taihu Lake, a shallow eutrophic lake.

The co-occurrence networks and interactions of bacterial communities in sediments are highly variable with environmental factors, which are vital to the nutrient biogeochemical cycle, pollutants biodegradation, and microbial community stability in lake ecosystems. Although pollution gradients reflect environmental variation comprehensively, few studies have characterized the changes in co-occurrence networks and interactions of bacterial communities along sediment pollution gradients. In order to investigate the impact of pollution gradients on compositions, co-occurrence networks, and interactions of sedimentary microbial communities, we studied the bacterial communities in the sediments of a typical shallow eutrophic lake, Taihu Lake, along pollution gradients using 16S rRNA gene high-throughput sequencing technology. All the sediment sampling sites were classified into mild, moderate, and severe pollution groups according to the sediments' physicochemical properties. Our results showed that the taxon richness was lowest in the severe pollution group, and the diversity of species decreased with the level of pollution. The complexity of the co-occurrence network decreased as the level of pollution increased, and the severe pollution group was characterized by a small-world network. The relative abundance of Proteobacteria, Bacteroidetes, and Chlorobi increased significantly as the level of pollution increased (P < 0.05). Strong inter-phyla co-occurrence or co-exclusion patterns demonstrated that the strength of interactions was enhanced in the severe pollution group, indicating stronger cooperative or competitive relationships. Chloroflexales and Chlorobiales were unique keystone taxa in the severe pollution group. The results of this study indicate that severe pollution reduces microbial diversity and network complexity, which may lead to community instability. The competition for nutrients of some copiotrophic bacteria may be enhanced as the level of pollution increased. The unique keystone taxa may contribute to photosynthesis and pollutant degradation in the severe pollution group. These findings expand our understanding of variation in bacterial co-occurrence networks and interactions along sediment pollution gradients.

Understanding the ecological processes governing hydrophyte-associated bacterial communities involved in hydrophyte growth and development.

Maintaining hydrophyte growth has been a major focus of aquatic ecological research. The hydrophyte microbiome plays a key role in the growth and health of hydrophytes, but the ecological processes regulating the assembly and function of hydrophyte microbial communities remain unclear. This knowledge gap limits the efficacy of managing microbiomes to enhance the capacity of hydrophytes to restore the aquatic environment. Here, we sampled three typical hydrophytes (Ceratophyllum demersum, Nymphoides peltatum, and Potamogeton crispus) to study the ecological process governing hydrophyte-associated bacterial communities. The results demonstrated that hydrophyte-associated bacterial communities were affected more by the hydrophyte host species (HEEI = 2.40) than by the environment (HEEI = 1.00). The hydrophyte host species not only affected bacterial community assembly, but reduced the diversity and network complexity of the bacterial community relative to that of the environment. Furthermore, the core taxa of two hydrophytes were identified. Chryseobacterium was the core taxon of N. peltatum, and Burkholderia-Caballeronia-Paraburkholderia, Pseudolabrys, and Pajaroellobacter were the core taxa of P. crispus. The core taxa of P. crispus were closely related to potential denitrification-related functions of bacteria and revealed that P. crispus played a role in denitrification during aquatic ecological restoration. Overall, the results of this study highlight the need to develop approaches employing hydrophyte-associated bacteria to promote the development of hydrophytes, which will be essential for increasing the utility of hydrophyte microbiomes in the future and enhancing aquatic ecological restoration.

Predicting bend-induced heterogeneity in sediment microbial communities by integrating bacteria-based index of biotic integrity and supervised learning algorithms.

Prioritizing the relationship between heterogeneity of sediment habitats and river bends is critical when planning and reconstructing urban rivers. However, the exact relationship between ecological heterogeneity and river bends remains ambiguous. Therefore, this research proposed a new approach to quantify and predict bend-induced ecological heterogeneity, incorporating the bacteria-based index of biotic integrity (Ba-IBI), path model, and random forest regression model. The developed Ba-IBI quantified heterogeneity in sediment microbial communities, ranging from low (1.40) to high (3.97). A path model was developed and validated in order to further investigate the relative contributions of environmental factors to the Ba-IBI. The established path model, which was considered acceptable with a CMIN/df = 1.949 < 4, suggested that primary environmental factors affecting the sediment bacterial communities were flow velocity and ammonium concentration in sediment. To further characterize the relationship between environmental factors and the Ba-IBI, a function was constructed using the random forest regression model that predicts the responses of sediment bacterial communities to environmental factors with R2 = 0.6126. The proposed approach and prediction tools will provide knowledge to improve natural channel design and post-project evaluations in river restoration projects.

Coupling Genomics and Hydraulic Information to Predict the Nitrogen Dynamics in a Channel Confluence.

The simulation of nitrogen dynamics in urban channel confluences is essential for the evaluation and improvement of water quality. The omics-based modeling approaches that have been rapidly developed have been increasingly applied to characterize metabolisms of the microbial community and transformation of the associated materials. However, the transport of microorganisms and chemicals within and among different phases, which could be the rate-limiting step for the nitrogen dynamics, are always neglected or oversimplified in omics-based models. Therefore, this study proposes a novel simulation system coupling genomic and hydraulic information to simulate transport and transformation processes and provide predictions of nitrogen dynamics in a confluence. The proposed model was able to capture multiphase mass transport, microbial population dynamics, and nitrogen transformation and accurately predict gene abundances and nitrogen concentrations in both water and sediment; the mean relative errors were all lower than 40%. The model emphasized the importance of transport processes, which contributed more than 90% to gene abundances and chemical concentrations. Moreover, the simulation of reaction rates exhibited the specific nitrogen transformation processes in the confluence. The sulfide oxidation and the nitrate reduction and anaerobic ammonium oxidation, with the participation of the genes nap and hzo, respectively, were promoted as the main processes of nitrate and nitrite reduction.