RESUMO
Closure of the ductus arteriosus requires prenatal formation of intimal cushions, which occlude the vessel lumen at birth. Survival of newborns with severe congenital heart defects, however, depends on ductal patency. We used a gene transfer approach to create a patent ductus arteriosus by targeting the fibronectin-dependent smooth muscle cell migration required for intimal cushion formation. Fetal lamb ductus arteriosus was transfected in utero with hemagglutinating virus of Japan liposomes containing plasmid encoding 'decoy' RNA to sequester the fibronectin mRNA binding protein. Fibronectin translation was inhibited and intimal cushion formation was prevented. We thus established the essential role of fibronectin-dependent smooth muscle cell migration in intimal cushion formation in the intact animal and the feasibility of incorporating biological engineering in the management of congenital heart disease.
Assuntos
Permeabilidade do Canal Arterial/genética , Fibronectinas/genética , Fibronectinas/fisiologia , Terapia Genética/métodos , Transfecção/métodos , Animais , Movimento Celular/genética , Modelos Animais de Doenças , Permeabilidade do Canal Arterial/embriologia , Permeabilidade do Canal Arterial/cirurgia , Feminino , Vetores Genéticos , Cardiopatias Congênitas/mortalidade , Cardiopatias Congênitas/patologia , Cardiopatias Congênitas/terapia , Lipossomos , Músculo Liso Vascular/citologia , Plasmídeos , Gravidez , Biossíntese de Proteínas , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Respirovirus , OvinosRESUMO
BACKGROUND: Leukocyte infiltration and serine elastase activity lead to smooth muscle cell proliferation in association with posttransplant coronary arteriopathy and may also be involved in vein graft neointimal formation. A number of therapies have targeted cellular proliferation, but the inhibition of serine elastase-mediated extracellular matrix remodeling has not been investigated as a potential strategy to prevent neointimal formation and subsequent atherosclerotic degeneration in vein grafts. METHODS AND RESULTS: We studied jugular vein grafts 48 hours after interposition into the carotid arteries of rabbits and demonstrated inflammatory cell infiltration and elevated serine elastase activity, a stimulus for matrix remodeling and deposition of elastin. Therefore, elastolytic activity in vein grafts was targeted through transient expression of the selective serine elastase inhibitor elafin with hemagglutinating virus of Japan liposome-mediated gene transfer. Elafin transfection reduced inflammation by 60% at 48 hours and neointimal formation by approximately 50% at 4 weeks after implantation. At 3 months, a 74% decrease in neointimal elastin deposition correlated with protection against cholesterol-induced macrophage infiltration and lipid accumulation, which were both reduced by approximately 50% in elafin-transfected grafts relative to controls. CONCLUSIONS: Gene transfer of the selective serine elastase inhibitor elafin in vein grafts is effective in reducing the early inflammatory response. Although transient expression of elafin delays neointimal formation, it is also sufficient to cause an alteration in elastin content of the extracellular matrix, making it relatively resistant to atherosclerotic degeneration.
Assuntos
Oclusão de Enxerto Vascular/prevenção & controle , Veias Jugulares/transplante , Proteínas/administração & dosagem , Proteínas/genética , Inibidores de Serina Proteinase/administração & dosagem , Inibidores de Serina Proteinase/genética , Animais , Arteriosclerose/enzimologia , Arteriosclerose/patologia , Arteriosclerose/prevenção & controle , Artérias Carótidas/cirurgia , Modelos Animais de Doenças , Elastina/metabolismo , Matriz Extracelular/enzimologia , Técnicas de Transferência de Genes , Oclusão de Enxerto Vascular/enzimologia , Oclusão de Enxerto Vascular/patologia , Imuno-Histoquímica , Veias Jugulares/efeitos dos fármacos , Veias Jugulares/enzimologia , Lipossomos , Proteínas Secretadas Inibidoras de Proteinases , Proteínas/metabolismo , Coelhos , Respirovirus/genética , Inibidores de Serina Proteinase/metabolismo , Transfecção , Túnica Íntima/efeitos dos fármacos , Túnica Íntima/metabolismo , Túnica Íntima/patologiaRESUMO
OBJECTIVE: Treatment options for patients with advanced pulmonary vascular disease caused by a congenital heart defect are still mainly limited to heart-lung transplantation or lung transplantation with repair of the cardiac lesion. Because we have previously shown that the structural changes associated with pulmonary hypertension can be reversed by stress unloading in an organ culture model, we now investigate whether hemodynamic unloading will lead to regression of pulmonary vascular disease in the intact animal. METHODS: Right middle and lower lobectomy and monocrotaline injection were performed in Lewis rats (n = 22) to cause pulmonary vascular disease from a combined hemodynamic and toxic injury. Twenty-eight days later the left lungs were examined (n = 10) or exposed to normal pulmonary artery pressure for an additional 14 (n = 5) or 28 (n = 7) days by transplantation into healthy recipients. Pulmonary artery pressure, ventricular weight, and pulmonary artery morphology were evaluated in each group. RESULTS: Pulmonary hypertension (50 vs 16 mm Hg; P <.001) and right ventricular hypertrophy (right ventricular/left ventricular weight 0.69 vs 0.32; P <.001) associated with pulmonary artery medial hypertrophy (28.2% vs 7.2% wall thickness; P <.001) and muscularization of small pulmonary arteries (92.3% vs 19.4%; P <.001) developed by day 28 (compared with untreated controls). However, transplantation into healthy recipients effectively unloaded the lungs (mean pulmonary artery pressure 17 and 24 mm Hg at 14 and 28 days after transplantation) and resulted in progressive normalization of medial hypertrophy (15.6% and 12.1% at 14 and 28 days) and muscularization (65.1% and 42.2% at 14 and 28 days) relative to nontransplanted controls (P <.005 in each case). CONCLUSIONS: Hemodynamic unloading of lungs with pulmonary vascular disease results in progressive normalization of pulmonary artery structure. These results are the first to provide a rationale for attempting to induce regression of pulmonary vascular disease by pressure unloading of the pulmonary circulation. Methods to mechanically unload the pulmonary circulation should be critically evaluated as a strategy for staged surgical repair of congenital heart defects despite presumed irreversible pulmonary hypertension.
Assuntos
Hipertensão Pulmonar/cirurgia , Hipertrofia Ventricular Direita/cirurgia , Pneumopatias Obstrutivas/cirurgia , Transplante de Pulmão , Artéria Pulmonar/cirurgia , Animais , Divisão Celular , Hipertensão Pulmonar/patologia , Hipertensão Pulmonar/fisiopatologia , Hipertrofia/patologia , Hipertrofia/fisiopatologia , Hipertrofia/cirurgia , Hipertrofia Ventricular Direita/patologia , Hipertrofia Ventricular Direita/fisiopatologia , Pneumopatias Obstrutivas/patologia , Pneumopatias Obstrutivas/fisiopatologia , Artéria Pulmonar/patologia , Artéria Pulmonar/fisiopatologia , Ratos , Resistência VascularRESUMO
We report a case of anomalous origin of left anterior descending coronary artery in a patient with tetralogy of Fallot, absent pulmonary valve cusps, and origin of left pulmonary artery from the ascending aorta. Pulmonary outflow tract obstruction was successfully treated by direct anastomosis of the main pulmonary artery to the right ventricle. This approach prevented injury to the anomalous coronary artery and avoided the use of an extracardiac conduit.
Assuntos
Anomalias dos Vasos Coronários/complicações , Tetralogia de Fallot/cirurgia , Anastomose Cirúrgica , Procedimentos Cirúrgicos Cardíacos/métodos , Pré-Escolar , Ventrículos do Coração/cirurgia , Humanos , Masculino , Artéria Pulmonar/cirurgia , Tetralogia de Fallot/complicaçõesRESUMO
BACKGROUND: The outcome of surgical correction of complete atrioventricular septal defect with tetralogy of Fallot has improved in recent years. Controversy exists about the optimal approach to this complex lesion. Our experience over the past 8 years with a single technique is reviewed. The important anatomic features of this lesion are discussed in relation to our method of repair. METHODS: Between 1988 and 1996, 11 consecutive patients underwent correction of complete atrioventricular septal defect with tetralogy of Fallot. Nine patients had undergone prior palliative shunts. The two-patch technique for atrioventricular septal defect was used. The ventricular septal defect was closed through a right ventriculotomy in each case. The commissure between the superior and inferior bridging leaflets of the left portion of the common atrioventricular valve was closed in each patient. Management of the right ventricular outflow tract was individualized. RESULTS: There was one mortality in the early postoperative period. One patient required reoperation for closure of a dehiscent left atrioventricular valve cleft. All survivors are currently in New York Heart Association functional class I or II at follow-up ranging from 2 to 101 months. CONCLUSIONS: Atrioventricular septal defect with tetralogy of Fallot can be corrected with low mortality using the two-patch technique and closure of the ventricular septal defect through a combined approach using a right ventriculotomy and right atriotomy. Routine closure of the commissure of the left portion of the atrioventricular valve results in a low incidence of regurgitation. A good functional result can be achieved in most patients at intermediate-term follow-up.