Projected frontal area and its components during front crawl depend on lung volume.

We examined the influence of lung volume on the vertical body position, trunk inclination, and projected frontal area (PFA) during swimming and the inter-relationships among these factors. Twelve highly trained male swimmers performed a 15 m front crawl with sustained maximal inspiration (INSP), maximal expiration (EXP), and intermediate (MID) at a target velocity of 1.20 m·s-1 . Using our developed digital human model, which allows inverse kinematics calculations by fitting individual body shapes measured with a three-dimensional photonic image scanner to individually measured underwater motion capture data, vertical center of mass (CoM) position, trunk inclination, and PFA were calculated for each complete stroke cycle. In particular, the PFA was calculated by automatic processing of a series of parallel frontal images obtained from a reconstructed digital human model. The vertical CoM position was higher with a larger lung-volume level (p < 0.01). The trunk inclination was smaller in INSP and MID than in EXP (p < 0.01). PFA was smaller with a larger lung-volume level (p < 0.01). Additionally, there was a significant interaction of vertical CoM position and trunk inclination with PFA (p = 0.006). There was a negative association between PFA and vertical CoM position, and a positive association between PFA and trunk inclination less than the moderate vertical CoM position (each p < 0.05). These results obtained using our methodology indicate that PFA decreases with increasing lung volume due to an increase in vertical CoM position, and additionally due to a decrease in trunk inclination at low-to-moderate lung-volume levels.

Novel Method for Estimating Propulsive Force Generated by Swimmers' Hands Using Inertial Measurement Units and Pressure Sensors.

Propulsive force is a determinant of swimming performance. Several methods have been proposed to estimate the propulsive force in human swimming; however, their practical use in coaching is limited. Herein, we propose a novel method for estimating the propulsive force generated by swimmers' hands using an inertial measurement unit (IMU) and pressure sensors. In Experiment 1, we use a hand model to examine the effect of a hand-mounted IMU on pressure around the hand model at several flow velocities and water flow directions. In Experiment 2, we compare the propulsive force estimated using the IMU and pressure sensors (FIMU) via an underwater motion-capture system and pressure sensors (FMocap). Five swimmers had markers, pressure sensors, and IMUs attached to their hands and performed front crawl swimming for 25 m twice at each of nine different swimming speeds. The results show that the hand-mounted IMU affects the resultant force; however, the effect of the hand-mounted IMU varies with the flow direction. The mean values of FMocap and FIMU are similar (19.59 ± 7.66 N and 19.36 ± 7.86 N, respectively; intraclass correlation coefficient(2,1) = 0.966), and their waveforms are similar (coefficient of multiple correlation = 0.99). These results indicate that the IMU can estimate the same level of propulsive force as an underwater motion-capture system.

Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats.

Electrical stimulation (ES)-induced muscle contraction has multiple effects; however, mechano-responsiveness of bone tissue declines with age. Here, we investigated whether daily low-frequency ES-induced muscle contraction treatment reduces muscle and bone loss and ameliorates bone fragility in early-stage disuse musculoskeletal atrophy in aged rats. Twenty-seven-month-old male rats were assigned to age-matched groups comprising the control (CON), sciatic nerve denervation (DN), or DN with direct low-frequency ES (DN+ES) groups. The structural and mechanical properties of the trabecular and cortical bone of the tibiae, and the morphological and functional properties of the tibialis anterior (TA) muscles were assessed one week after DN. ES-induced muscle contraction force mitigated denervation-induced muscle and trabecular bone loss and deterioration of the mechanical properties of the tibia mid-diaphysis, such as the stiffness, but not the maximal load, in aged rats. The TA muscle in the DN+ES group showed significant improvement in the myofiber cross-sectional area and muscle force relative to the DN group. These results suggest that low-frequency ES-induced muscle contraction treatment retards trabecular bone and muscle loss in aged rats in early-stage disuse musculoskeletal atrophy, and has beneficial effects on the functional properties of denervated skeletal muscle.

Electrical Stimulation of Denervated Rat Skeletal Muscle Ameliorates Bone Fragility and Muscle Loss in Early-Stage Disuse Musculoskeletal Atrophy.

We tested whether daily muscle electrical stimulation (ES) can ameliorate the decrease in cortical bone strength as well as muscle and bone geometric and material properties in the early stages of disuse musculoskeletal atrophy. 7-week-old male F344 rats were randomly divided into three groups: age-matched control group (Cont); a sciatic denervation group (DN); and a DN + direct electrical stimulation group (DN + ES). Denervated tibialis anterior (TA) muscle in the DN + ES group received ES with 16 mA at 10 Hz for 30 min/day, 6 days/week. Micro CT, the three-point bending test, and immunohistochemistry were used to characterize cortical bone mechanical, structural, and material properties of tibiae. TA muscle in the DN + ES group showed significant improvement in muscle mass and myofiber cross-sectional area relative to the DN group. Maximal load and stiffness of tibiae, bone mineral density estimated by micro CT, and immunoreactivity of DMP1 in the cortical bone tissue were also significantly greater in the DN + ES group than in the DN group. These results suggest that daily ES-induced muscle contraction treatment reduced the decrease in muscle mass and cortical bone strength in early-stage disuse musculoskeletal atrophy and is associated with a beneficial effect on material properties such as mineralization of cortical bone tissue.

Augmented Carbohydrate Oxidation under Moderate Hypobaric Hypoxia Equivalent to Simulated Altitude of 2500 m.

Hypoxia itself stimulates glucose uptake mediated by a mechanism independent of insulin. However, whether moderate hypoxia causes similar metabolic effect in humans remains unclear. The present study aimed to determine glycemic regulation following glucose load at a simulated moderate altitude of 2,500 m. Eight healthy young males (mean ± standard error: 24 ± 1 years; 171.3 ± 1.6 cm; 66.9 ± 3.7 kg; 22.8 ± 1.0 kg/m(2)) consumed 75 g of glucose solution under either hypobaric condition (560 mmHg) or normobaric condition (745 mmHg). In the hypobaric chamber, the oxygen partial pressure is proportionally reduced with a reduction of atmospheric pressure, consequently leading to the hypoxic condition. Plasma glucose and serum insulin concentrations increased significantly following glucose load in both conditions (P < 0.05). However, no significant interaction (condition × time) or main effect for condition was observed. There were no significant differences in serum glycerol, plasma epinephrine, or plasma norepinephrine concentrations between the two conditions. No significant differences between the conditions were observed in changes in VO2 or VCO2. However, the hypobaric condition showed significantly higher respiratory exchange ratio (VCO2/VO2) at 90 and 120 min following glucose load (P < 0.05 vs. normobaric condition), suggesting that carbohydrate oxidation following glucose load was enhanced in moderate hypobaric hypoxia. In conclusion, acute exposure to moderate hypobaric hypoxia significantly augmented carbohydrate oxidation following the glucose load, without affecting glucose or insulin responses. Thus, a short-time exposure to moderate hypobaric hypoxia may be beneficial for people with impaired glucose tolerance.

Nutritional Practice and Nitrogen Balance in Elite Japanese Swimmers during a Training Camp.

The protein requirement in athletes increases as a result of exercise-induced changes in protein metabolism. In addition, the frequency, quantity, and quality (i.e., leucine content) of the protein intake modulates the protein metabolism. Thus, this study aimed to investigate whether nutritional practice (particularly, protein and amino acid intake at each eating occasion) meets the protein needs required to achieve zero nitrogen balance in elite swimmers during a training camp. Eight elite swimmers (age 21.9 ± 2.3 years, body weight 64.2 ± 7.1 kg, sex M:2 F:6) participated in a four-day study. The nitrogen balance was calculated from the dietary nitrogen intake and urinary nitrogen excretion. The amino acid intake was divided over six eating occasions. The nitrogen balance was found to be positive (6.7 ± 3.1 g N/day, p < 0.05) with protein intake of 2.96 ± 0.74 g/kg/day. The frequency and quantity of leucine and the protein intake were met within the recommended range established by the International Society of Sports Nutrition. Thus, a protein intake of 2.96 g/kg/day with a well-designated pattern (i.e., frequency throughout the day, as well as quantity and quality) of protein and amino acid intake may satisfy the increased need for protein in an elite swimmer.

Lower lung-volume level induces lower vertical center of mass position and alters swimming kinematics during front-crawl swimming.

We examined the impact of lung-volume levels on the vertical center of mass (CoM) position and kinematics during submaximal front-crawl swimming at constant velocity. Thirteen well-trained male swimmers (21.2 ± 2.0 years) swam the front-crawl for 15 m at a target velocity of 1.20 m s-1 while holding one of three lung-volume levels: maximal inspiration (MAX), maximal expiration (MIN), and intermediate between these (MID). The three-dimensional positions of 25 reflective markers attached to each participant's body were recorded using an underwater motion capture system and then used to estimate the body's CoM. The swimming velocity and the vertical CoM position relative to the water's surface were calculated and averaged for one stroke cycle. Stroke rate, stroke length, kick rate, kick amplitude, kick velocity, and trunk inclination were also calculated for one stroke cycle. Swimming velocity was statistically comparable among the three different lung-volume levels (ICC [2,3] = 0.875). The vertical CoM position was significantly decreased with the lower lung-volume level (MAX: -0.152 ± 0.009 m, MID: -0.163 ± 0.009 m, MIN: -0.199 ± 0.007 m, P < 0.001). Stroke rate, kick rate, kick amplitude, kick velocity, and trunk inclination were significantly higher in MIN than in MAX and MID, whereas the stroke length was significantly lower (all P < 0.05). These results indicate that a lower lung-volume level during submaximal front-crawl swimming induces a lower vertical CoM position that is accompanied by a modulation of the swimming kinematics to overcome the increased drag arising from a larger projected frontal area.

Does a jammer-type racing swimsuit improve sprint performance during maximal front-crawl swimming?

We investigated the effects of jammer-type racing swimsuits (RS) on swimming performance during arm-stroke-only (pull) and whole-body stroke (swim) in 25-m front-crawl with maximal effort. Twelve well-trained male collegiate swimmers wore RS and a conventional swimsuit (CS) and performed three tests: pull, swim, and pull using the system to measure active drag (MAD pull). Swimming velocity and intra-abdominal pressure (IAP) were determined in all tests. Stroke indices during pull and swim and drag-swimming velocity relationship and maximum propulsive power during MAD pull were also determined. Swimming velocities during pull and swim while wearing an RS (1.59 ± 0.13 and 1.77 ± 0.09 m·s-1, respectively) were significantly higher than those wearing a CS (1.57 ± 0.14 and 1.74 ± 0.08 m·s-1, respectively). Stroke length during pull and swim was significantly greater while wearing an RS (1.68 ± 0.12 and 1.83 ± 0.13 m, respectively) than wearing a CS (1.63 ± 0.10 and 1.81 ± 0.13 m, respectively). However, no significant differences were confirmed between the other variables in all tests. In conclusion, swimming performance is improved when wearing an RS compared with a CS.

Metabolic and cardiovascular responses to upright cycle exercise with leg blood flow reduction.

The purpose of this study was to examine the metabolic and cardiovascular response to exercise without (CON) or with (BFR) restricted blood flow to the muscles. Ten young men performed upright cycle exercise at 20, 40, and 60% of maximal oxygen uptake, VO2max in both conditions while metabolic and cardiovascular parameters were determined. Pre-exercise VO2 was not different between CON and BFR. Cardiac output (Q) was similar between the two conditions as a 25% reduction in stroke volume (SV) observed in BFR was associated with a 23% higher heart rate (HR) in BFR compared to CON. As a result rate-pressure product (RPP) was higher in the BFR but there was no difference in mean arterial pressure (MAP) or total peripheral resistance (TPR). During exercise, VO2 tended to increase with BFR (~10%) at each workload. Q increased in proportion to exercise intensity and there were no differences between conditions. The increase in SV with exercise was impaired during BFR; being ~20% lower in BFR at each workload. Both HR and RPP were significantly greater at each workload with BFR. MAP and TPR were greater with BFR at 40 and 60% VO2max. In conclusion, the BFR employed impairs exercise SV but central cardiovascular function is maintained by an increased HR. BFR appears to result in a greater energy demand during continuous exercise between 20 and 60% of control VO2max; probably indicated by a higher energy supply and RPP. When incorporating BFR, HR and RPP may not be valid or reliable indicators of exercise intensity. Key pointsBlood flow reduction (BFR) employed impairs stroke volume (SV) during exercise, but central cardiovascular function is maintained by an increased heart rate (HR).BFR appears to result in a greater energy demand during continuous exercise between 20 and 60% of control VO2max;Probably indicated by a higher energy supply (VO2) and rate-pressure product (HR x systolic blood pressure).

The effect of using paddles on hand propulsive forces and Froude efficiency in arm-stroke-only front-crawl swimming at various velocities.

Through pressure measurement and underwater motion capture analysis, this study aimed to elucidate the effects of hand paddles on hand propulsive forces, mechanical power, and Froude efficiency in arm-stroke-only front-crawl swimming at various velocities. Eight male swimmers swam under two conditions in randomized order, once using only their hands and once aided by hand paddles on both hands. Each participant swam 10 times a distance of 16 m in each condition, for a total of 20 trials. To elucidate the relationship between propulsive forces and swimming velocity, each participant was instructed to swim each of the two sets of 10 trials at an arbitrarily different swimming velocity. During the trials, pressure sensors and underwater motion capture cameras were used together to analyze the pressure forces acting on the hand and hand kinematics, respectively. Six pressure sensors were attached to the right hand, and pressure forces acting on the right hand were estimated by multiplying the areas with the pressure differences between the palm and dorsal side of the hand. Acting directions of pressure forces were analyzed using a normal vector perpendicular to the hand or hand paddle, calculated from coordinates obtained using underwater motion capture analysis. As a result, there were no differences in propulsive forces and mechanical power to overcome water resistance (PD) with or without hand paddles at the same swimming velocities. However, the use of hand paddles decreased stroke rate and hand velocities, so mechanical power to push the water at the hand (PK) decreased. Using hand paddles thus increased Froude efficiency (ηF). These results suggest that training load decreases when swimmers swim at the same velocities while using hand paddles. This insight could prove useful for coaches and swimmers when using hand paddles for training to help ensure that they are used in accordance with their intended training purpose. If swimmers use hand paddles increasing propulsive force or PK, they should swim at a higher swimming velocity with hand paddles than without.

Metabolic responses to exercise on land and in water following glucose ingestion.

Although aerobic exercise after a meal decreases postprandial blood glucose, the differences in glucose response between land and aquatic exercise are unclear. Thus, we examined the effect of different modes of exercise with same energy expenditure following glucose ingestion on carbohydrate metabolism. Ten healthy sedentary men (age, 22 ± 1 years) participated in this study. All subjects performed each of three exercise modes (cycling, walking and aquatic exercise) for 30 min after ingestion of a 75-g glucose solution with 1-2 weeks between trials. The exercise intensity was set at 40% of the maximum oxygen uptake that occurred during cycling. The velocity during walking and the target heart rate during aquatic exercise were predetermined in a pretest. The plasma glucose concentration at 30 min after exercise was significantly lower with aquatic exercise compared to that with cycling and walking (P<0·05). However, there were no significant differences among the three exercise modes in respiratory exchange ratio. On the other hand, serum free fatty acid concentration with aquatic exercise was significantly higher at 120 min after exercise compared with that after walking (P<0·05). These results suggest that aquatic exercise reduces postprandial blood glucose compared with both cycling and walking with the same energy expenditure. Aquatic exercise shows potential as an exercise prescription to prevent postprandial hyperglycaemia.

Within-Day Amino Acid Intakes and Nitrogen Balance in Male Collegiate Swimmers during the General Preparation Phase.

A higher protein intake is recommended for athletes compared to healthy non-exercising individuals. Additionally, the distribution and quality (i.e., leucine content) of the proteins consumed throughout the day should be optimized. This study aimed to determine the nitrogen balance and distribution of protein and amino acid intakes in competitive swimmers during the general preparation phase. Thirteen swimmers (age: 19.7 ± 1.0 years; VO2max: 63.9 ± 3.7 mL·kg-1·min-1, mean ± standard deviation) participated in a five-day experimental training period. Nutrient intakes were assessed using dietary records. Nitrogen balance was calculated from the daily protein intake and urinary nitrogen excretion. The intake amounts of amino acids and protein at seven eating occasions were determined. The average and population-safe intakes for zero nitrogen balance were estimated at 1.43 and 1.92 g·kg-1·day-1, respectively. The intake amounts of protein and leucine at breakfast, lunch, and dinner satisfied current guidelines for the maximization of muscle protein synthesis, but not in the other four occasions. The population-safe protein intake level in competitive swimmers was in the upper range (i.e., 1.2⁻2.0 g·kg-1·day-1) of the current recommendations for athletes. The protein intake distribution and quality throughout the day may be suboptimal for the maximization of the skeletal muscle adaptive response to training.

The effect of paddles on pressure and force generation at the hand during front crawl.

Through pressure measurement, this study aimed to clarify the effects of hand paddle use on pressure and force generation around the hand during the front crawl. Eight male swimmers performed two trials of front crawl swimming with maximal effort, once using only their hands and once aided by hand paddles. During trials, pressure sensors and underwater motion capture cameras were used together to analyze hand kinematics and pressure forces acting on the hand. Six pressure sensors were attached to the right hand, and pressure forces acting on the right hand were estimated by multiplying the areas and the pressure differences between the palm side and dorsal side of the hand. Acting directions of pressure forces were analyzed using a normal vector perpendicular to the hand, calculated from coordinates of the right hand. As a result, using hand paddles decreases pressure differences between the palm and dorsal sides of hand related to the magnitude of pressure force. However, no difference was found in the mean value of resultant pressure forces compared with using hands alone, because the large surface area of the hand paddle compensated the decreased pressure differences due to decreased hand velocity. In addition, when hand paddles were used, the component of the pressure force acting in propulsive direction was significantly higher. Thus, the ratio of forces acting in the propulsive direction was higher than without hand paddles. These results suggest that the training loads with hand paddles are not high even if the swimming velocities increase because the power generated by upper limb motion didn't increase.

Effects of Streptomycin Administration on Increases in Skeletal Muscle Fiber Permeability and Size Following Eccentric Muscle Contractions.

The purpose of this study was to investigate the preventive effect of streptomycin (Str) administration on changes in membrane permeability and the histomorphological characteristics of damaged muscle fibers following eccentric contraction (ECC ). Eighteen 7-week-old male Fischer 344 rats were randomly assigned to three groups: control (Cont), ECC, and ECC with Str (ECC + Str). The tibialis anterior (TA) muscles in both ECC groups were stimulated electrically and exhibited ECC. Evans blue dye (EBD), a marker of muscle fiber damage associated with increased membrane permeability, was injected 24 hr before TA muscle sampling. The number of EBD-positive fibers, muscle fiber cross-sectional area (CSA), and roundness were determined via histomorphological analysis. The ECC intervention resulted in an increased fraction of EBD-positive fibers, a larger CSA, and decreased roundness. The fraction of EBD-positive fibers was 79% lower in the ECC + Str group than in the ECC group. However, there was no difference in the CSA and roundness of the EBD-positive fibers between the two ECC groups. These results suggest that Str administration can reduce the number of myofibers that increase membrane permeability following ECC, but does not ameliorate the extent of fiber swelling in extant EBD-positive fibers. Anat Rec, 301:1096-1102, 2018. © 2018 Wiley Periodicals, Inc.

Electrical Stimulation of Denervated Rat Skeletal Muscle Retards Capillary and Muscle Loss in Early Stages of Disuse Atrophy.

The purpose of the present study is to investigate the effects of low-frequency electrical muscle stimulation (ES) on the decrease in muscle mass, fiber size, capillary supply, and matrix metalloproteinase (MMP) immunoreactivity in the early stages of denervation-induced limb disuse. Direct ES was performed on the tibialis anterior muscle following denervation in seven-week-old male rats. The rats were divided into the following groups: control (CON), denervation (DN), and denervation with direct ES (DN + ES). Direct ES was performed at an intensity of 16 mA and a frequency of 10 Hz for 30 min per day, six days a week, for one week. We performed immunohistochemical staining to determine the expression of dystrophin, CD34, and MMP-2 in transverse sections of TA muscles. The weight, myofiber cross-sectional area (FCSA), and capillary-to-fiber (C/F) ratio of the tibialis anterior (TA) muscle were significantly reduced in the DN group compared to the control and DN + ES groups. The MMP-2 positive area was significantly greater in DN and DN + ES groups compared to the control group. These findings suggest beneficial effects of direct ES in reducing muscle atrophy and capillary regression without increasing MMP-2 immunoreactivity in the early stages of DN-induced muscle disuse in rat hind limbs.

The effects of exercise training under mild hypoxic conditions on body composition and circulating adiponectin in postmenopausal women.

PURPOSE: This study aimed to examine the effects of exercise training under mild hypoxic conditions on body composition and circulating adiponectin levels in postmenopausal women. METHODS: Fourteen postmenopausal women (56 ± 1 years) were assigned to a normoxic (N group) or hypoxic (H group) exercise group. Aquatic exercise training was performed at an intensity of 50% peak oxygen uptake level for 30 min per training session, 4 days per week, for 8 weeks. The H group performed the exercise under hypobaric hypoxic conditions, which corresponds to 2000 m above sea level, and each participant was exposed to these conditions for 2 h per session. RESULTS: After the training, no significant changes were observed in any of the measured values for the N group. Conversely, body mass (57·3 ± 2·5 to 54·5 ± 2·3 kg), body mass index (24·6 ± 0·8 to 23·4 ± 0·7 kg m-2 ), body fat (30·7 ± 1·9 to 28·1 ± 1·6%) and preperitoneal fat thickness as an index of visceral fat accumulation (10·3 ± 1·7 to 6·4 ± 1·0 mm) significantly reduced only in the H group. Circulating adiponectin levels significantly increased (9·5 ± 1·8 to 11·4 ± 2·0 µg ml-1 ), and the changes in adiponectin were significantly correlated with those in body mass (r = -0·81) and body mass index (r = -0·85). CONCLUSIONS: These results suggest that exercise training under mild hypoxic conditions could more effectively reduce body fat and increase adiponectin levels in postmenopausal women in a shorter period, than exercise training in normoxia.

Effects of resistance training under hypoxic conditions on muscle hypertrophy and strength.

It has been reported that exercise under hypoxic conditions elevates acute growth hormone secretion after exercise compared with that under normoxic conditions. This study examined the influence of resistance training under moderate hypoxic conditions on muscle thickness, strength and hormonal responses. Thirteen healthy men were assigned into two groups matched for physical fitness level and then randomized into two groups that performed exercise under normoxic (FiO2  = 20·9%) or hypoxic (FiO2  = 12·7%) conditions. Three sets of elbow extensions with unilateral arm were performed to exhaustion at a workload of a 10 repetition maximum with 1-minute intervals for 3 days per week for 8 weeks. The thickness of the biceps and triceps brachii was determined using B-mode ultrasound before and after training. Blood sampling was carried out before and after exercise, as well as during the first and last training sessions. Increase in the thickness of the triceps brachii in trained arm was significantly greater in the hypoxic group than in the normoxic group. The 10 repetition maximum was significantly increased not only in the trained arm but also in the untrained arm in both groups. Serum growth hormone concentrations after exercise were significantly higher in the hypoxic group than in the normoxic group on both the first and last training sessions. These findings suggest that hypoxic resistance training elicits more muscle hypertrophy associated with a higher growth hormone secretion, but that the greater muscle hypertrophy did not necessarily contribute a greater gain of muscle strength.

Reduced neural drive in bilateral exertions: a performance-limiting factor?

PURPOSE: Activity of the motor cortex in one hemisphere reduces the maximum motor outflow of homologous parts of the opposite hemisphere, causing a reduction in the maximum force a muscle can exert when the contralateral homologous muscle is activated concurrently. The purpose of this study was to establish whether this bilateral deficit is large enough to explain limitations in performance in bilateral exertions. METHODS: Voluntary force production and neural drive during unilateral and bilateral exertions were compared in three experiments, consisting of unilateral maximum contractions, synchronous bilateral contractions, and asynchronous bilateral contractions of finger flexors and knee extensors. RESULTS: Maximum voluntary force was overall about 7% lower in bilateral knee extension as compared with unilateral knee extension (P < 0.001). In finger flexion, a bilateral voluntary force deficit of as much as 20% was found ( P= 0.001). Corresponding deficits in agonist EMG activity were also significant and on average found to be of similar size, though the magnitude of the bilateral deficit in EMG was not consistently related to the magnitude of the bilateral force deficit. In knee extension, a deficit in voluntary activation of 4% (P = 0.003) was demonstrated by means of superimposed tetanic stimulation. The magnitude of this deficit was correlated to the magnitude of the voluntary force deficit (r = 0.80, P= 0.002). The maximum rate of force development in bilateral knee extensions was 13% lower than in a unilateral knee extension (P = 0.002). CONCLUSION: These results suggest that deficits in bilateral force production are large enough to constitute an important performance-limiting factor. Furthermore, the data suggest that a reduced neural drive underlies this bilateral deficit.

Effects of gravitational loading levels on protein expression related to metabolic and/or morphologic properties of mouse neck muscles.

The effects of 3 months of spaceflight (SF), hindlimb suspension, or exposure to 2G on the characteristics of neck muscle in mice were studied. Three 8-week-old male C57BL/10J wild-type mice were exposed to microgravity on the International Space Station in mouse drawer system (MDS) project, although only one mouse returned to the Earth alive. Housing of mice in a small MDS cage (11.6 × 9.8-cm and 8.4-cm height) and/or in a regular vivarium cage was also performed as the ground controls. Furthermore, ground-based hindlimb suspension and 2G exposure by using animal centrifuge (n = 5 each group) were performed. SF-related shift of fiber phenotype from type I to II and atrophy of type I fibers were noted. Shift of fiber phenotype was related to downregulation of mitochondrial proteins and upregulation of glycolytic proteins, suggesting a shift from oxidative to glycolytic metabolism. The responses of proteins related to calcium handling, myofibrillar structure, and heat stress were also closely related to the shift of muscular properties toward fast-twitch type. Surprisingly, responses of proteins to 2G exposure and hindlimb suspension were similar to SF, although the shift of fiber types and atrophy were not statistically significant. These phenomena may be related to the behavior of mice that the relaxed posture without lifting their head up was maintained after about 2 weeks. It was suggested that inhibition of normal muscular activities associated with gravitational unloading causes significant changes in the protein expression related to metabolic and/or morphological properties in mouse neck muscle.

Vascular adaptations to hypobaric hypoxic training in postmenopausal women.

The objective of this study was to examine the effects of exercise training in hypoxia on arterial stiffness and flow-mediated vasodilation (FMD) in postmenopausal women. Sixteen postmenopausal women (56±1 years) were assigned to a normoxic exercise group (Normoxic group, n=8) or a hypoxic exercise group (Hypoxic group, n=8). The Hypoxic group performed exercise under hypobaric hypoxic conditions corresponding to 2000 m above sea level, and was exposed to these conditions for 2 h per session. Aquatic exercise was performed at an intensity of around 50% peak oxygen uptake for 30min, 4days per week, for 8 weeks. Arterial stiffness was assessed by brachial-ankle pulse wave velocity (baPWV), and FMD was evaluated by peak diameter of the popliteal artery during reactive hyperemia. After the 8 weeks of training, the Normoxic group showed no significant changes. In contrast, baPWV (P < 0.05) was significantly reduced and peak diameter (P<0.05) and %FMD (P<0.01) were significantly increased in the Hypoxic group after training. These results suggest that exercise training under mild intermittent hypoxic conditions could more effectively reduce arterial stiffness in postmenopausal women, compared with exercise training performed at the same relative intensity under normoxic conditions. Our data also indicate that hypoxic exercise training may induce vascular functional adaptation, for example an increase in FMD response. These findings therefore could have important implications for the development of a new effective exercise prescription program.