RESUMO
It is well known that Brownian motion can be described using Langevin equation. In this paper we extend the application of the Langevin equation to the Rayleigh-Bénard (RB) flow, assuming that each molecule in the system is a Brownian particle colliding with its surrounding molecules. The phenomenon of thermal instability, changing from a conductive to a convective state, is well reproduced by Langevin dynamics simulations. The roles of the drag force and the random force terms in the Langevin equation in triggering thermal instability are elucidated via numerical tests. Furthermore, we demonstrate that the strength of the fluctuation correlations increases as the Rayleigh number approaches the critical value, and the characteristics of the fluctuation correlations below the onset of instability foreshadow the form of the convective patterns emerging above the critical point. The Langevin equation, together with the form of the fluctuation correlations, sheds new light on the mechanism of the RB instability.
RESUMO
Many microbial pathogens invade and proliferate within host cells and the molecular mechanism underlying this behavior is currently being revealed for several bacterial species. Testing clinically relevant antibacterial compounds and elucidating their effects on gene expression requires adequate controls, especially when studying genetically intractable organisms such as Chlamydia spp., for which various gene fusions cannot be constructed. Until now, relative mRNA levels in Chlamydia have been measured using different internal gene expression controls, including 16S rRNA, mRNAs, and DNA. Here, we compared the advantages and disadvantages of various internal expression controls during the early phase of Chlamydia pneumoniae development. The relative abundance of target mRNAs varied using the different internal control RNAs. This was partly due to variation in the transcript stability of the RNA species. Also, seven out of nine of the analyzed RNAs increased fivefold or more between 2 and 14 h postinfection, while the amount of DNA and number of cells remained essentially unaltered. Our results suggest that RNA should not be used as a gene expression control during the early phase of Chlamydia development, and that intrinsic bacterial DNA is preferable for that purpose because it is stable, abundant, and its relative amount is generally correlated with bacterial numbers.