RESUMO
The cytosolic and two recombinant precursors, containing 10 and 30 amino acid spacers between the transit peptide and the mature region of the chloroplast flavoprotein ferredoxin-NADP+ reductase (FNR), were expressed in Escherichia coli cells. These proteins were purified rendering fully active precursors that contained bound FAD. Neither the transit peptide nor the spacers affected the formation of the tightly folded enzyme structure. Protease treatment of the folded precursors resulted in a rapid removal of the transit sequence, rendering an enzymatically active resistant core, even at high protease concentration. All three preproteins could be efficiently imported by isolated pea chloroplasts. Addition of the enzyme substrate NADP+ to the import medium slightly decreased the polypeptide translocation. The precursor bound to isolated chloroplasts in the presence or absence of leaf extracts was as resistant to proteolysis as the folded precursor in solution. In contrast, the FNR precursor unfolded by urea was rapidly digested even at the lowest protease concentration. Together, our results indicate that precursor unfolding may take place during translocation but not during binding to chloroplast envelopes or by interaction with leaf extract soluble factors, and that this process is independent of the distance between the transit peptide and the folded mature region of the protein.
Assuntos
Cloroplastos/enzimologia , Precursores Enzimáticos/química , Precursores Enzimáticos/metabolismo , Ferredoxina-NADP Redutase/química , Ferredoxina-NADP Redutase/metabolismo , Flavina-Adenina Dinucleotídeo/química , Sequência de Aminoácidos , Sequência de Bases , Transporte Biológico Ativo , Primers do DNA/genética , Precursores Enzimáticos/genética , Escherichia coli/genética , Ferredoxina-NADP Redutase/genética , Expressão Gênica , Membranas Intracelulares/enzimologia , Pisum sativum/enzimologia , Pisum sativum/genética , Reação em Cadeia da Polimerase , Dobramento de Proteína , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
In ferredoxin-NADP(+) reductase (FNR), FAD is bound outside of an anti-parallel beta-barrel with the isoalloxazine lying in a two-tyrosine pocket. To elucidate the function of the flavin si-face tyrosine (Tyr-89 in pea FNR) on the enzyme structure and catalysis, we performed ab initio molecular orbital calculations and site-directed mutagenesis. Our results indicate that the position of Tyr-89 in pea FNR is mainly governed by the energetic minimum of the pairwise interaction between the phenol ring and the flavin. Moreover, most of FNR-like proteins displayed geometries for the si-face tyrosine phenol and the flavin, which correspond to the more negative free energy theoretical value. FNR mutants were obtained replacing Tyr-89 by Phe, Trp, Ser, or Gly. Structural and functional features of purified FNR mutants indicate that aromaticity on residue 89 is essential for FAD binding and proper folding of the protein. Moreover, hydrogen bonding through the Tyr-89 hydroxyl group may be responsible of the correct positioning of FAD and the substrate NADP(+)
Assuntos
Ferredoxina-NADP Redutase/química , Ferredoxina-NADP Redutase/metabolismo , Pisum sativum/enzimologia , Tirosina/química , Tirosina/metabolismo , Animais , Sítios de Ligação , DNA Complementar/metabolismo , Escherichia coli/metabolismo , Humanos , Ligação de Hidrogênio , Cinética , Modelos Moleculares , Mutagênese Sítio-Dirigida , Mutação , NADP/química , Oxigênio/metabolismo , Fenol/química , Ligação Proteica , Estrutura Secundária de Proteína , Espectrofotometria , Especificidade por Substrato , Termodinâmica , Fatores de TempoRESUMO
The flavoprotein ferredoxin-NADP+ reductase catalyzes the final step of the photosynthetic electron transport i.e., the reduction of NADP+ by ferredoxin. Expression and secretion of this enzyme was examined in Saccharomyces cerevisiae using a cDNA cloned from a pea library [Newman, B. J. & Gray, J. C. (1988) Plant Mol. Biol. 10, 511-520]. Two pea library cDNA sequences were employed, one corresponding to the mature enzyme and the other containing, in addition, the sequence of the transit peptide that directs ferredoxin-NADP+ reductase to the chloroplast. These sequences were introduced into a yeast shuttle vector in frame with the mating factor alpha 1 secretion-signal coding region under the control of its natural mating factor alpha 1 promoter. Saccharomyces cerevisiae cells transformed with the recombinant plasmids were able to synthesize and secrete fully active pea ferredoxin-NADP+ reductase. In both cases, a 35-kDa polypeptide was the major product. N-terminal sequencing of the secreted proteins indicates processing at position -1 with respect to the N-terminus of the pea mature enzyme. Yeast cells transformed with plasmid encoding the ferredoxin-NADP+ reductase precursor secrete four-times more ferredoxin-NADP+ reductase to the medium than cells transformed with the plasmid encoding the mature form of the enzyme. Ferredoxin-NADP+ reductases purified from culture medium showed structural and enzymatic properties that were identical, within the experimental error, to those of native plant ferredoxin-NADP+ reductase. The overall results indicate that pea ferredoxin-NADP+ reductase can be properly folded and its prosthetic group assembled in the yeast endoplasmic reticulum, and that its natural transit peptide favors its secretion.
Assuntos
Ferredoxina-NADP Redutase/genética , Ferredoxina-NADP Redutase/metabolismo , Regulação Enzimológica da Expressão Gênica , Plantas/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , Eletroforese em Gel de Poliacrilamida , Precursores Enzimáticos/genética , Precursores Enzimáticos/metabolismo , Ferredoxina-NADP Redutase/biossíntese , Ferredoxina-NADP Redutase/química , Dados de Sequência Molecular , Peso Molecular , Plasmídeos , Dobramento de Proteína , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/genéticaRESUMO
The contribution made by tyrosine 308 to the stability of pea ferredoxin-NADP+ reductase was investigated using site-directed mutagenesis. The phenol side chain of the invariant carboxyl terminal tyrosine is stacked coplanar to the isoalloxazine moiety of the FAD cofactor. Fluorescence measurements indicate that this interaction plays a significant role in FAD fluorescent quenching by the reductase apoprotein. Replacement of the tyrosine by tryptophan or phenylalanine caused only a minor increase in the quantum yields of bound FAD, whereas nonaromatic substitutions to serine and glycine resulted in a large fluorescent rise. Results from NADP+ titration experiments support a recent hypothesis [Karplus et al. (1991) Science 251, 60-66], suggesting that the phenol ring of Tyr 308 may fill the nicotinamide binding pocket in the absence of the nucleotide. The stability of the site-directed mutants, judged by thermal- and urea-induced denaturation studies, was lowered with respect to the wild-type enzyme. FNR variants harboring nonaromatic substitutions displayed more extensive destabilization. The decrease in thermodynamic stability correlated with the impairment of catalytic activities [Orellano et al. (1993) J. Biol. Chem 268, 19267-19273]. The results indicate that the presence of the electron-rich aromatic side chain adjacent to the isoalloxazine ring is essential for maximum stabilization of the FNR holoenzyme, resulting in a flavin conformation which optimizes electron flow between the prosthetic group and its redox partners.
Assuntos
Ferredoxina-NADP Redutase/metabolismo , Flavina-Adenina Dinucleotídeo/metabolismo , Pisum sativum/enzimologia , Tirosina/metabolismo , Sítios de Ligação , Estabilidade Enzimática , Ferredoxina-NADP Redutase/química , Ferredoxina-NADP Redutase/genética , Temperatura Alta , Mutação , Desnaturação Proteica , Espectrometria de Fluorescência , Tirosina/químicaRESUMO
The precursor of the chloroplast flavoprotein ferredoxin-NADP+ reductase from pea was expressed in Escherichia coli as a carboxyl-terminal fusion to glutathione S-transferase. The fused protein was soluble, and the precursor could be purified in a few steps involving affinity chromatography on glutathione-agarose, cleavage of the transferase portion by protease Xa, and ion exchange chromatography on DEAE-cellulose. The purified prereductase contained bound FAD but displayed marginally low levels of activity. Removal of the transit peptide by limited proteolysis rendered a functional protease-resistant core exhibiting enzymatic activity. The FAD-containing precursor expressed in E. coli was readily transported into isolated pea chloroplasts and was processed to the mature size, both inside the plastid and by incubation with stromal extracts in a plastid-free reaction. Import was dependent on the presence of ATP and was stimulated severalfold by the addition of plant leaf extracts.
Assuntos
Precursores Enzimáticos/metabolismo , Ferredoxina-NADP Redutase/metabolismo , Pisum sativum/enzimologia , Sequência de Bases , Transporte Biológico Ativo , Cloroplastos/enzimologia , DNA Complementar/genética , DNA de Plantas/genética , Precursores Enzimáticos/química , Precursores Enzimáticos/genética , Escherichia coli/genética , Ferredoxina-NADP Redutase/química , Ferredoxina-NADP Redutase/genética , Flavina-Adenina Dinucleotídeo/química , Vetores Genéticos , Dados de Sequência Molecular , Pisum sativum/genética , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production of NADPH during photosynthesis. Whereas the structures of FNRs from spinach leaf and a cyanobacterium as well as many of their homologs have been solved, none of these studies has yielded a productive geometry of the flavin-nicotinamide interaction. Here, we show that this failure occurs because nicotinamide binding to wild type FNR involves the energetically unfavorable displacement of the C-terminal Tyr side chain. We used mutants of this residue (Tyr 308) of pea FNR to obtain the structures of productive NADP+ and NADPH complexes. These structures reveal a unique NADP+ binding mode in which the nicotinamide ring is not parallel to the flavin isoalloxazine ring, but lies against it at an angle of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.