RESUMO
High levels of background γ-radiation exist in the suburbs of Baku, Azerbaijan. We examined the impact of radiation on erythrocyte nuclear pathologies, levels of cytochrome P-450, and serotonin-modulating anticonsolidation protein (SMAP) in the tissues of the hens from three settlements with different levels of background radiation. Higher levels of radiation resulted in increased nuclear pathologies, upregulation of tissue SMAP levels, and downregulation of cytochrome P-450. We also carried out controlled dosage studies on Wistar male rats, which showed significant upregulation of heat shock proteins with molecular mass 70 kDa (HSP70) in the bone marrow 3 and 5 h after SMAP intraperitoneal administration. Administration of SMAP to rats 3 h prior to γ-radiation exposure (8 Gy) provided significant protection to somatic cell nuclei. We conclude that SMAP can provide protection from the genotoxic effects of γ-radiation through upregulation of HSP70 or the transformation of chromatin into a condensed, more protective conformational state.
Assuntos
Radiação de Fundo , Galinhas , Animais , Azerbaijão , Núcleo Celular/metabolismo , Galinhas/fisiologia , Sistema Enzimático do Citocromo P-450 , Eritrócitos , Feminino , Masculino , Ratos , Ratos Wistar , Serotonina/metabolismoRESUMO
The work present data on studies of a decreased activity of serotoninergic system on the level of mutagenic changes (the micronuclear test) in the goby Neogobius fluviatilis and the fry if sturgeon Acipenser güldenstädti persicus. It has been shown that the long exposure of the animals to conditions of industrial and oil pollution leads to a significant decrease in their liver of the level of serotonin-modulated anticonsolidation protein (SMAP) correlating directly with the serotonin level as well as to sharp increase of the level of micronuclei in erythrocytes. The intramuscular administration of anti-SMAP polyclonal antibodies to the fry of the sturgeon produces a significant increase of the amount of micronuclei as compared with that in the animals injected with non-immune gamma-globulin. The obtained results allow concluding that the decrease of activity of the serotoninergic system is the mechanism that is triggered with adverse environmental factors and realizes mutagenic damages in the modified genetic apparatus.
Assuntos
Anticorpos/farmacologia , Proteínas de Peixes/antagonistas & inibidores , Peixes/metabolismo , Mutagênese/efeitos dos fármacos , Mutação , Petróleo/efeitos adversos , Poluentes Químicos da Água/efeitos adversos , Animais , Eritrócitos/metabolismo , Proteínas de Peixes/metabolismo , Peixes/genética , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Músculo Esquelético/metabolismo , Serotonina/metabolismoAssuntos
Sistema Nervoso Central/fisiologia , Potenciais Evocados , Peixes/fisiologia , Nervo Olfatório/fisiologia , Condutos Olfatórios/fisiologia , Animais , Potenciais Evocados/efeitos dos fármacos , Bulbo Olfatório/fisiologia , Condutos Olfatórios/efeitos dos fármacos , Parassimpatolíticos/farmacologia , Parassimpatomiméticos/farmacologiaRESUMO
We studied changes in the content of serotonin-modulated anticonsolidation protein in the liver of goldfishes and gobies caused by oil and industrial pollution. The concentration of serotonin-modulated anticonsolidation protein in fish liver increased after short-term exposure to oil-contaminated water (100 mg/liter), but decreased under long-term effect of industrial wastes. We hypothesize that serotonin plays a role in antimutagenic protection of the organism and maintains the differentiated state of mature cells.