Effect of sewage sludge derived compost or biochar amendment on the phytoaccumulation of potentially toxic elements and radionuclides by Chinese cabbage.

This study set out to evaluate the effect of using sewage sludge-derived compost (SSC) or biochar (SSB) as a soil amendment on the phytoaccumulation of potentially toxic elements, PTE (Cd, Cr, Cu, Ni, Pb, Zn) and natural radionuclides (238U and 232Th) by Chinese cabbage (Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) in terra rossa and rendzina soils, which are the two common soil types in Croatia. The experiment consisted of a greenhouse pot trial using a three-factor design where soil type, sludge post-stabilisation procedure and amendment rate (12 and 120 mgP/L) were the main factors. At harvest, the concentrations of analytes in the substrate, leaves and roots were measured, from which the edible tissue uptake (ETU) and concentration ratios (CR) were determined. Also, the average daily dose (ADD) and hazard quotient (HQ) were determined to assess the health risk, as well as soil contamination factor (CF). The results showed that neither adding SSC nor SSB affected the soil loading at the rates applied, suggesting a low risk of soil contamination (CF ≤ 1). The ETU of Cd, Cu, and Zn were 0.0061, 1.23, and 0.91 mg/plant from compost-amended soil and 0.0046, 0.78 and 0.65 mg/plant for biochar-amended soil, respectively. This difference suggests that their ETU was higher in compost-amended soils than in soils treated with biochar. The CR data indicate that the bioavailability of Cu (CR of 5.30) is highest at an amendment rate of 12 mgP/L, while for Zn (CR of 0.69), the highest bioaccumulation was observed with an amendment rate of 120 mgP/L. Translocation of Cr, Ni, Pb and 238U to the leaves was limited. Overall, the HQ (<1) for Cd, Cu and Zn in the edible parts confirmed that consuming Chinese cabbage does not threaten human health. Similarly, the daily intake of 232Th remained below the limit (3 µg) set by ICRP, suggesting no radiological risk. Finally, although the amendment rate, which was 10-times the amount stipulated in Croatian regulation and the CR ranged from 0.007 to 5.30, the precautionary principle is advised, and the long-term impact of sewage sludge derived compost or biochar on different plant groups (incl. root vegetables) at the field-scale is recommended.

Effect of olive-processing technology on the utilization of olive mill pomace as a soil amendment.

In the Mediterranean basin, the treatment and disposal of olive mill pomace (OMP) remain a salient environmental issue for the olive oil-producing industry. This study assesses the effects of olive-processing technology (three-phase and two-phase systems) on the potential use of OMP as a soil amendment. Samples from 12 Croatian olive mills were analyzed for their total phenolic content (TPC), residual oil fraction, and elemental concentration. The samples were profiled using Fourier transform infrared spectroscopy (FT-IR) and structurally characterized using scanning electron microscopy-energy-dispersive X-ray spectroscopy (EDS). Compared to three-phase samples, two-phase OMP was more acidic (pH 4.5 vs. 5.0), with a higher TPC (3835 vs. 1576 mg/kg fresh weight), oil content (11.7% vs. 7.5% d.w., where d.w. is dry weight), electrical conductivity (EC, 5.1 vs. 3.0 mS/cm), and levels of calcium (Ca, 1.34 vs. 1.20 g/kg d.w.) and copper (Cu, 10.4 vs. 7.0 mg/kg d.w.). Similar values of carbon/nitrogen (C/N; 61 vs. 72), N (10 vs. 8.1 g/kg d.w.), phosphorus (1040 vs. 691 mg/kg d.w.), and potassium (K, 13.7 vs. 8.1 g/kg d.w.) were observed. The amounts of chromium, copper, nickel, and zinc were below EC limits in both cases. The EDS mapping revealed that Ca was concentrated at sharp-edged OMP particles while K was evenly distributed, suggesting that pelletized OMP compost is preferable for amending soil to obtain a homogeneous distribution of nutrients. It was also possible to distinguish between OMPs based on oil and lignin absorption bands in their FT-IR spectra. According to the obtained results, composting is recommended for both types of OMP to produce a safe product for amendment purposes.

The Phytochemical and Nutritional Composition of Shallot Species (Allium × cornutum, Allium × proliferum and A. cepa Aggregatum) Is Genetically and Environmentally Dependent.

Shallots are a perennial plant from the Alliaceae family, classified with the common onion under the name of the Allium cepa Aggregatum group. The term shallot is also used for diploid and triploid viviparous onions, known as Allium × proliferum (Moench) Schrad and Allium × cornutum Clementi ex Vis., respectively. In this study, we compared the dry matter, pyruvic acid content, sugar content, flavonoid content, antioxidant capacity and mineral composition of 34 shallot accessions falling into three shallot species (Allium × cornutum, Allium × proliferum and A. cepa Aggregatum). Shallot accessions belonging to the A.× cornutum and A. × proliferum groups are characterized by high dry matter content (around 25%), of which a little less than 50% is formed of inulin-type sugars, polysaccharides, considered an excellent prebiotic with beneficial effects on human health. On the other hand, accessions belonging to the A. cepa Aggregatum group have lower dry matter content and, as a result, lower pungency (measured as pyruvic acid content), making them more suitable for fresh consumption by a broader range of consumers, but, at the same time, abundant in phenolic compounds, especially quercetin and isorhamnetin glycosides. We also observed a greater biodiversity among accessions within the A. cepa Aggregatum group in all the analyzed physico-chemical parameters compared to the other shallot groups. The investigated shallot accessions have an excellent in vitro antioxidant capacity, as well as excellent nutritional properties.

Shallot Species and Subtypes Discrimination Based on Morphology Descriptors.

Shallots are an edible Alliaceous crop representing a group of genetically and morphologically different species. Shallot species determination is rather complex due to the high variability in phenotypes within a single species. Flower morphology has been successfully employed in shallot species determination; however, shallot florogenesis depends upon many genetic and environmental factors. There is a need for more accessible morphological descriptors used in shallot species determination, since flowering in shallot may not be consistent. In this study, we investigated the discriminating power of shallot vegetative and bulb morphology descriptors. European Cooperative Programme for Plant Genetic Resources morphology descriptors were used for describing 35 Croatian shallot accessions. The proposed methodology based on vegetative and bulb morphological descriptors could be used for shallot species discrimination. Additionally, two subtypes of A. cepa Aggregatum group were identified in this study: the first being the shallot type (1) and a potato onion type (2), which differed based on bulb morphology descriptors (bulb shape, bulb skin color, and a number of bulblets).

The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile.

The extraction of glucosinolates in boiling aqueous methanol from freeze dried leaf tissues is the most common method for myrosinase inactivation but can be hazardous because of methanol toxicity. Although freeze drying is the best dehydration method in terms of nutritional quality preservation, the main drawbacks are a limited sample quantity that can be processed simultaneously, a long processing time, and high energy consumption. Therefore, the aim of this study is to evaluate the effects of applying high temperature for myrosinase inactivation via hot air drying prior to the extraction step, as well as the effects of cold aqueous methanol extraction on total antioxidant activity, total glucosinolates, total phenolic content, and sugar profile in 36 landraces of kale. The results from our study indicate that cold aqueous methanol can be used instead of boiling aqueous methanol with no adverse effects on total glucosinolate content. Our results also show that hot air drying, compared to freeze drying, followed by cold extraction has an adverse effect on antioxidant activity measured by DPPH radical scavenging, total glucosinolate content, as well as on the content of all investigated sugars.

Morphological and Biochemical Diversity of Shallot Landraces Preserved Along the Croatian Coast.

Shallots are a valuable minor Allium crop, and are propagated vegetatively and maintained in home gardens across generations along the Croatian coast and island areas. Shallot landraces growing along the Croatian coast fall into three genotypes: Allium cepa Aggregatum group (2n = 2x = 16), A. × proliferum (Moench) Schard. (2n = 2x = 16), and A. × cornutum Clementi ex Vis. (2n = 3x = 24), among which A. × cornutum is the most widespread. The aim of this study was to differentiate shallot accessions collected from local farmers using morphological markers. Also, the chemical composition including phenolic content, phenolic profile, total antioxidant capacity, and mineral composition, of shallot accessions was compared with that of the local landraces of common onion, and with market available shallot and common onion cultivars. Based on morphological observations and using multivariate classification, shallot landraces were classified into three distinct groups. Properties, based on which A. × cornutum can be differentiated from A. cepa Aggregatum and A. × proliferum, are stamen morphology, stamen length, leaf and scape vegetative properties, number of bulbs in cluster, cluster mass, and bulb diameter. Flower diameter and flower pedicel length differentiate A. × cornutum and A. × proliferum from A. cepa Aggregatum. Significant variability was observed in the biochemical profiles across tested accessions. Compared with the commercial common onion cultivars, local shallot accessions have higher bulb N, P, and K content. The major phenolic compounds identified in shallots were quercetin-4'-glucoside and quercetin-3,4'-diglucoside. Additionally, several other minor phenolic compounds were also identified. Morphological and biochemical profiles were evaluated using Partial Least Square (PLS) analysis. Specific morphological traits and biochemical markers for possible species identification are proposed.