RESUMO
We examined the ability of two filarial species, Onchocerca volvulus and Brugia malayi, to solubilize collagen molecules from native collagen fibrils. Collagenolytic activity was detected in extracts of adult worms, in living microfilariae of O. volvulus and in live infective larvae and adult female worms of B. malayi. Excretion-secretion factors produced in vitro by infective larvae of B. malayi also contained large amounts of collagenase. Studies with enzyme inhibitors suggest that the latter may be a metallo-protease. Antibodies to filarial collagenase were present in sera from patients with onchocerciasis and brugian filariasis and from mice immunized with B. malayi. These antibodies and a monoclonal antibody raised against O. volvulus antigens immunoprecipitate filarial collagenase but appear not to be directed against the active site of the enzyme.
Assuntos
Antígenos de Helmintos , Brugia/enzimologia , Colagenase Microbiana/metabolismo , Onchocerca/enzimologia , Animais , Anticorpos/análise , Anticorpos/imunologia , Antígenos de Helmintos/imunologia , Brugia/imunologia , Colágeno/metabolismo , Eletroforese em Gel de Poliacrilamida , Filariose Linfática/imunologia , Epitopos/imunologia , Feminino , Humanos , Camundongos , Colagenase Microbiana/antagonistas & inibidores , Colagenase Microbiana/imunologia , Microfilárias , Onchocerca/imunologia , Oncocercose/imunologia , Pele/parasitologiaRESUMO
We used a noncompetitive two-site ELISA with 5 monoclonal antibodies to determine whether parasite antigens are present in breast milk from women infected with Onchocerca volvulus. Seven out of 13 available milk samples contained significant amounts of filarial antigens. Antigen indices in milk correlated with levels of microfilarodermia (Rs = 0.74, P less than 0.005). Antigen-containing milk samples markedly inhibited mitogen-induced proliferation of human mononuclear cells and activated cells within this population that suppressed the proliferative response of autologous lymphocytes to mitogens and antigens. These findings indicate that parasite products are present in breast milk of O. volvulus-infected women and suggest that these may induce immune tolerance and/or suppression in infants born of infected mothers.
Assuntos
Antígenos de Helmintos/análise , Leite Humano/imunologia , Onchocerca/imunologia , Oncocercose/imunologia , Animais , Anticorpos Anti-Helmínticos/análise , Anticorpos Monoclonais , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Tolerância Imunológica , Ativação LinfocitáriaRESUMO
To determine whether chemotherapy effectively reduces Plasmodium falciparum malaria transmission in isolated human populations, we followed two abrupt sequential outbreaks of malaria infection among Yanomami Amerindians and modeled the effect of chemotherapy and the consequences if no drug was available. A Macdonald-type mathematical model demonstrated that both outbreaks comprised a single epidemic event linked by an invisible outbreak in vector mosquitoes. The basic reproductive number, R0, from fitted values based on the treated epidemic was 2 during the initial phase of the epidemic, and waned as vector density decreased with the onset of the dry season. In the observed epidemic, 60 (45%) of 132 village residents were affected, and the treated outbreak ended after two months. Although the initial chemotherapy regimen was only marginally effective, the duration of human infectivity was reduced from an expected nine months to two weeks. In the absence of this intervention, the initial R0 value would have been 40, more than 60% of the population would have been infected, and more than 30% would have remained parasitemic until the next rainy season (about six months later). Another outbreak would then have ensued, and malaria probably would have remained endemic in this village. Our simulated placebo treatment permits us to conclude that even partially effective chemotherapeutic interventions, such as those in our study, interrupt serial transmission of P. falciparum among isolated human populations that are exposed to infection seasonally.
Assuntos
Antimaláricos/uso terapêutico , Indígenas Sul-Americanos , Malária Falciparum/tratamento farmacológico , Malária Falciparum/transmissão , Animais , Surtos de Doenças , Humanos , Malária Falciparum/epidemiologia , Modelos Biológicos , Parasitemia/parasitologia , Placebos , Plasmodium falciparum/isolamento & purificação , Chuva , População Rural , Estações do AnoRESUMO
To determine whether malaria perpetuates within isolated Amerindian villages in the Venezuelan Amazon, we surveyed malaria infection and disease among 1,311 Yanomami in three communities during a 16-month period. Plasmodium vivax was generally present in each of these small, isolated villages; asymptomatic infection was frequent, and clinical disease was most evident among children less than five years of age (odds ratio [OR] = 6.3, 95% confidence interval [CI] = 1.4-29.2) and among persons experiencing parasitemias > or = 1,000 parasites/mm3 of blood (OR = 45.0, 95% CI = 5.5-370.7). Plasmodium falciparum, in contrast, was less prevalent, except during an abrupt outbreak in which 72 infections resulted in symptoms in all age groups and at all levels of parasitemia, and occasionally were life-threatening. The observed endemic pattern of P. vivax infection may derive from the capacity of this pathogen to relapse, while the epidemic pattern of P. falciparum infection may reflect occasional introductions of strains carried by immigrants or residents of distant villages and the subsequent disappearance of this non-relapsing pathogen.
Assuntos
Indígenas Sul-Americanos , Malária Falciparum/epidemiologia , Malária Vivax/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Animais , Anopheles/parasitologia , Criança , Pré-Escolar , Estudos Transversais , Feminino , Humanos , Lactente , Recém-Nascido , Malária Falciparum/parasitologia , Malária Vivax/parasitologia , Masculino , Parasitemia/parasitologia , Plasmodium falciparum/isolamento & purificação , Plasmodium vivax/isolamento & purificação , Estudos Prospectivos , Chuva , População Rural , Estações do Ano , Venezuela/epidemiologiaRESUMO
We have examined the reproducibility, sensitivity, and specificity of detecting Plasmodium falciparum using the polymerase chain reaction (PCR) and the species-specific probe pPF14 under field conditions in the Venezuelan Amazon. Up to eight samples were field collected from each of 48 consenting Amerindians presenting with symptoms of malaria. Sample processing and analysis was performed at the Centro Amazonico para la Investigacion y Control de Enfermedades Tropicales Simon Bolivar. A total of 229 samples from 48 patients were analyzed by PCR methods using four different P. falciparum-specific probes. One P. vivax-specific probe and by conventional microscopy. Samples in which results from PCR and microscopy differed were reanalyzed at a higher sensitivity by microscopy. Results suggest that microscopy-negative, PCR-positive samples are true positives, and that microscopy-positive and PCR-negative samples are true negatives. The sensitivity of the DNA probe/PCR method was 78% and its specificity was 97%. The positive predictive value of the PCR method was 88%, and the negative predictive value was 95%. Through the analysis of multiple blood samples from each individual, the DNA probe/PCR methodology was found to have an inherent reproducibility that was highly statistically significant.
Assuntos
DNA de Protozoário/sangue , Malária Falciparum/diagnóstico , Plasmodium falciparum/isolamento & purificação , Reação em Cadeia da Polimerase , Animais , Sequência de Bases , Primers do DNA/química , Sondas de DNA , DNA de Protozoário/química , Humanos , Indígenas Sul-Americanos , Malária Falciparum/sangue , Malária Vivax/sangue , Malária Vivax/diagnóstico , Dados de Sequência Molecular , Plasmodium falciparum/genética , Plasmodium vivax/genética , Plasmodium vivax/isolamento & purificação , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Especificidade da Espécie , VenezuelaRESUMO
Leishmaniasis is on the increase in Venezuela (ca 30,000 new cases per year) due to deterioration in health management, increased risk groups among inmunosuppressed individuals and increased human penetration into the ecological habitats of sandfly vectors. An STD2-funded project (1989-1992) focused on the Andean state of Táchira, which showed the highest annual index of new cases (ca 200-250). The project aimed at contributing to vector/parasite identification through a combination of molecular and well established field techniques: Newly developed molecular methods distinguished among Lu. spinicrassa, Lu. youngi and Lu. townsendi. These three species of the Verrucarum group are sympatric in the Northeast of the state and could be successfully identified by CHA, DNA probes and RAPD. A Le. braziliensis specific KDNA probe used with squash blots indicated that Lu. spinicrassa is the main vector and that Le. braziliensis is the main parasite species in Táchira state, Venezuela. PCR and the Le. brasiliensis specific DNA probe, schizodemes, isoenzymes and polyclonal antibodies agreed as taxonomic criteria for classification of Leishmania isolated from parasitologically confirmed cases in Tachira. Considerable degree of antigen heterogeneity in Venezuelan Le. braziliensis complex and Le. mexicana complex isolates from Tachira suggests multiple candidate antigens for improving the specificity of immunological diagnosis. The methods developed and tested in Táchira state should be valuable in order to help solving other outstanding epidemiological problems such as following of the epidemiological impact of intervention and vector control measures in highly endemic areas. Future work (STD3 funded, 1993-1996) aims to apply these molecular techniques to a vector control pilot study in Lara state, an area showing the highest incidence of new cases in the country.
Assuntos
Insetos Vetores , Leishmania/genética , Leishmaniose Cutânea/epidemiologia , Leishmaniose Cutânea/genética , Epidemiologia Molecular/métodos , Vigilância da População , Psychodidae , Animais , Impressões Digitais de DNA/métodos , Sondas de DNA , Estudos de Avaliação como Assunto , Heterogeneidade Genética , Humanos , Immunoblotting/métodos , Incidência , Controle de Insetos , Leishmania/classificação , Leishmania/imunologia , Leishmaniose Cutânea/diagnóstico , Leishmaniose Cutânea/prevenção & controle , Sondas Moleculares , Reação em Cadeia da Polimerase/métodos , Fatores de Risco , Sensibilidade e Especificidade , Venezuela/epidemiologiaRESUMO
We compared the chemical and immunological properties of cuticular collagens from four species of filarial nematodes, Onchocerca volvulus, O. gutturosa, Brugia malayi, and Dirofilaria immitis. The electrophoretic mobility of the major polypeptides extracted from adult worms is characteristic for each species studied. Cuticular collagens from adult worms and infective larvae differ in their susceptibility to proteases that cleave vertebrate collagens and to collagenases prepared from different developmental stages of filarial parasites. The overall amino acid composition of filarial collagens resembles that of vertebrate interstitial collagens and differs from that reported for collagens from free-living or intestinal nematodes. However, cuticular proteins of the four filarial species studied significantly differed in amino acid composition and in their reactivity with antisera to interstitial and basement membrane collagens of vertebrates.
Assuntos
Brugia/análise , Dirofilaria immitis/análise , Proteínas de Helminto/análise , Onchocerca/análise , Aminoácidos/análise , Animais , Antígenos de Helmintos/análise , Brugia/imunologia , Dirofilaria immitis/imunologia , Cães , Eletroforese em Gel de Poliacrilamida , Proteínas de Helminto/imunologia , Proteínas de Helminto/isolamento & purificação , Humanos , Imuno-Histoquímica , Onchocerca/imunologiaRESUMO
We examined the immunogenicity of various connective tissue proteins in patients with chronic onchocercal dermatitis and the effect of filarial proteases on this host-parasite interaction. Sera from patients with onchocerciasis reacted strongly with cuticular collagens from filarial parasites and with mammalian laminin. Some sera also contained antibodies to elastin and collagen type IV, but none reacted with collagen types I-III or fibronectin. This pattern of reactivity was characteristic for onchocerciasis: sera from patients with mansonellosis reacted strongly with collagen type IV but only weakly with laminin. Reactivity with mammalian laminin or collagen could not be absorbed with cuticular proteins from filarial worms and vice versa. Digestion fragments of laminin treated with filarial proteases retain antigenic determinants recognized by sera from patients with onchocerciasis. In contrast, proteases from Onchocerca volvulus adults and microfilariae drastically decreased the reactivity of the same sera with collagen type IV. These results indicate that filarial proteases may contribute to the pathogenesis of chronic onchocercal dermatitis, directly, by enzymatically destroying connective tissue of the skin, and indirectly, by triggering autoimmune responses to self-determinants on connective tissue proteins that are normally hidden within the supramolecular structure of the extracellular matrix complex.
Assuntos
Endopeptidases/metabolismo , Proteínas da Matriz Extracelular/imunologia , Onchocerca volvulus/enzimologia , Oncocercose/etiologia , Dermatopatias Parasitárias/etiologia , Animais , Anticorpos Anti-Helmínticos/sangue , Autoanticorpos/sangue , Colágeno/imunologia , Tecido Conjuntivo/imunologia , Tecido Conjuntivo/patologia , Epitopos/imunologia , Proteínas de Helminto/imunologia , Humanos , Soros Imunes/imunologia , Laminina/imunologia , Proteínas de Membrana/imunologia , Oncocercose/enzimologia , Oncocercose/imunologia , Dermatopatias Parasitárias/enzimologia , Dermatopatias Parasitárias/imunologiaRESUMO
The patterns of acid phosphatase in strains of Onchocerca volvulus s.l. which parasitize an Amerindian population (Yanomami) in Venezuela's Upper Orinoco Basin were examined by using the naphthol AS-TR phosphate method. The study sample consisted of 40 Yanomami inhabiting a savannah area at 950 m above sea level and 21 Yanomami residents of a tropical rainforest area at an altitude of 250 m. Stained intrauterine microfilariae, still within the egg case, exhibited a diffuse distribution of the enzyme in the early stages of embryonic development and a negative reaction at a more developed stage. Four of the five enzyme staining patterns described by Omar (1978) were found in the 3157 microfilariae examined from skin snips. Their distribution was: Type I--17.2%, Type III--0.5%, Type IV--75.6% and Type V--6.6%. No examples of Type II were observed. The results indicate that acid phosphatase patterns of the Upper Orinoco Onchocerca strain most resemble those of strains from Guatemala and Yemen, and are different from the African strains found in Upper Volta and Liberia. The relative frequency of acid phosphatase patterns was modified by cryopreservation of microfilariae.
Assuntos
Fosfatase Ácida/análise , Onchocerca/enzimologia , Oncocercose/parasitologia , Adolescente , Adulto , Animais , Criança , Feminino , Congelamento , Humanos , Indígenas Sul-Americanos , Microfilárias/enzimologia , Onchocerca/fisiologia , Óvulo/enzimologia , Pele/parasitologia , VenezuelaRESUMO
A sample of 121 Piaroa Indians from the Federal Amazonia Territory (Venezuela) was studied for the following serum protein polymorphisms: haptoglobin (HP), group-specific component subtypes (GC), orosomucoid (ORM), third component of complement (C3), transferrin C subtypes (TF) and alpha 1-antitrypsin subtypes (PI). The gene frequencies in the whole sample were: HP1 = 0.821; GC1S = 0.698; GC1F = 0.058; GC2 = 0.244; ORMS = 0.434; C3S = 0.699; C3F = 0.289; C3var = 0.012; TFC1 = 0.955; PIM1 = 0.467; PIM2 = 0.004; PIM3 = 0.529. The studied Piaroa sample came from three different communities: Gavilan, Paria and Alto Carinagua. The distribution of GC, C3 and HP polymorphisms was heterogeneous within the three groups. All the examined serum protein markers were polymorphic, in contrast to some enzymatic markers (ADA, DIA, 6PGD, AK) previously studied, which were shown to be monomorphic in the Piaroa. The results were compared with data from other populations living in the same territory.
Assuntos
Proteínas Sanguíneas/genética , Indígenas Sul-Americanos/genética , Análise de Variância , Feminino , Frequência do Gene , Marcadores Genéticos , Humanos , Masculino , Fenótipo , VenezuelaRESUMO
Phosphoglucomutase (PGM) and trehalase (Tre) isoenzymes of five species of Simulium blackflies (Diptera: Simuliidae), vectors of onchocerciasis in Venezuela, were investigated by means of a portable electrophoresis field kit. Tre differed between S. incrustatum and S. oyapockense s.l. Electrophoretic variation of Tre in other members of the S. amazonicum and S. incrustatum groups merit further investigation. PGM appears to be more useful for separating populations within species complexes. Multiple populations and/or seasonal changes in population structure of S. guianense s.l., S. exiguum s.l. and S. metallicum s.l. were inferred from elecrophoretic variation of PGM.
Assuntos
Isoenzimas/metabolismo , Onchocerca volvulus , Fosfoglucomutase/metabolismo , Simuliidae/parasitologia , Trealase/metabolismo , Animais , Eletroforese em Gel de Poliacrilamida/veterinária , Feminino , Simuliidae/classificação , Especificidade da Espécie , VenezuelaRESUMO
Malaria parasites are genetically diverse at all levels of endemicity. In contrast, the merozoite surface protein (MSP) alleles in samples from 2 isolated populations of Yanomami Amerindians during an epidemic of Plasmodium falciparum were identical. The nonvariable restriction fragment length polymorphism patterns further suggested that the sequential outbreak comprised only a single P. falciparum genotype. By examination of serial samples from single human infections, the MSP characteristics were found to remain constant throughout the course of infection. An apparent clonal population structure of parasites seemed to cause outbreaks in small isolated villages. The use of standard molecular epidemiologic methods to measure genetic diversity in malaria revealed the occurrence of a genetically monomorphic population of P. falciparum within a human community.
Assuntos
Surtos de Doenças , Indígenas Sul-Americanos , Malária Falciparum/etnologia , Malária Falciparum/parasitologia , Plasmodium falciparum/genética , Animais , Antígenos de Protozoários/genética , Southern Blotting , DNA de Protozoário/análise , Eletroforese em Gel de Ágar , Genes de Protozoários , Humanos , Proteína 1 de Superfície de Merozoito/genética , Epidemiologia Molecular , Plasmodium falciparum/crescimento & desenvolvimento , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Proteínas de Protozoários/genética , População Rural , Venezuela/epidemiologiaRESUMO
Cutaneous and mucocutaneous leishmaniases are widely spread in the mountainous Andean regions of South America. In Venezuela, these regions consist of the coffee-growing states of Trujillo, Merida and Tachira. Entomological and parasitological investigations in three geographically different climatic zones (Lomas Bajas, Delicias and La Grita) in Tachira state have shown a predominance of the sandfly species Lutzomyia spinicrassa (verrucarum group) and two Leishmania species, Leishmania mexicana and Leishmania braziliensis. Two transmission cycles appear to occur: a peridomestic cycle in Lomas Bajas and a sylvatic one in Delicias and La Grita.
Assuntos
Leishmaniose Cutânea/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Leishmania braziliensis/isolamento & purificação , Leishmania mexicana/isolamento & purificação , Leishmaniose Cutânea/parasitologia , Leishmaniose Mucocutânea/epidemiologia , Leishmaniose Mucocutânea/parasitologia , Pessoa de Meia-Idade , Prevalência , Chuva , Venezuela/epidemiologiaRESUMO
En el presente trabajo se analizaron extractos de antígenos empleando anti-cuerpos monoclonales, producidos por O. volvulus. La búsqueda de antígeno se realizó en muestras de suero y leche a través de técnicas de ELISA. Esto reveló que la enzima es inmunogénica en hospederos naturales (hombre) y experimentales (ratón), estimulando la aparición de anticuerpos contra la enzima no dirigidos a su centro activo