RESUMO
Cells of Escherichia coli contain two nucleoside-transport systems. Energy-starved cells of a strain containing only one of these systems and, in addition, carrying a mutation in the Ca2+- and Mg2+-dependent ATPase (ATP phosphohydrolase 3.6.1.3) are still able to transport nucleosides. The rate is only slightly lower than the rate measured in unstarved cells. Freshly harvested uncA cells transport purine nucleosides at a higher rate than cells from the isogenic strain containing a functional ATPase. If cells from the latter strain are treated with arsenate, transport rates increase to the same levels as found in uncA cells. The presence of an uncA mutation has no effect on the transport rates for cytidine, deoxycytidine, and uridine, nor has arsenate treatment. These findings indicate that ATP is not required as energy donor for nucleoside transport. The enhanced transport rate for purine nucleosides after treatment with arsenate seems to suggest a regulatory relationship between the transport of these nucleosides and the cellular levels of ATP or a closely related metabolite.
Assuntos
Trifosfato de Adenosina/metabolismo , Escherichia coli/metabolismo , Nucleosídeos de Purina/metabolismo , Trifosfato de Adenosina/deficiência , Arseniatos/farmacologia , Transporte Biológico Ativo/efeitos dos fármacos , ATPases Transportadoras de Cálcio/metabolismo , Proteínas de Transporte/metabolismo , Escherichia coli/genética , Guanosina/metabolismo , Mutação , Especificidade por SubstratoRESUMO
Osmotic shock treatment of cells of Escherichia coli K12 caused a reduction in the transport of nucleosides into the cells. The strains used carried mutations in the nucleoside catabolizing enzymes. This indicated that the decrease in transport capacity was not due to loss of these enzymes during the shock treatment. Membrane vesicles, prepared from the same strains, showed a limited transport of cytidine, deoxycytidine, and uridine. Transport of purine nucleosides and of thymidine was very low in vesicles lacking the appropriate nucleoside phosphorylases and no significant stimulation was observed if the nucleoside phosphorylases were present in the membrane vesicles. These results all indicate that components outside the cytoplasmic membrane are important for nucleoside transport. Selection for resistance to fluorodeoxycytidine yielded mutants which were unable to transport any nucleoside, even when the nucleoside phosphorylases were present in high amounts. This finding is consistent with a requirement for a specific transport process prior to the initial enzymatic attack on the incoming nucleoside.