RESUMO
Haemagglutinating, vibriocidal and mouse protective antibody responses in cholera patients were found to be maximum on the 7th day of admission. The mouse protective antibody on the first day at the hospital was lower than those of human volunteers. The circulating antibodies in the patients declined to normal levels or lower than normal before 3 months after the acute onset.
Assuntos
Anticorpos Antibacterianos/análise , Cólera/imunologia , Animais , Testes de Hemaglutinação , Humanos , Imunoglobulinas/análise , Fatores de TempoRESUMO
The heat-killed, phenolized parenteral typhoid vaccine was tested in informed volunteers. Assessment for its immunogenicity was performed using Widal test and enzyme-linked immunosorbent assay (ELISA). The anti-H antibody, which is a marker of the vaccine antigenicity peaked at one month after the vaccination and appeared throughout the one year course of the study. The anti-O antibody peaked at 7th day after vaccination and lasted only for 6 months. Classes of specific antibodies were determined by ELISA using single extracted lipopolysaccharide from Salmonella typhi 0901 as antigen. The possible protective role of serum derived intestinal IgG and IgA were discussed. Based on the agglutinating antibodies, the results indicate that the heat-killed, phenolized typhoid vaccine conferred at least 6 months protective period.
Assuntos
Anticorpos Antibacterianos/análise , Vacinas Bacterianas/imunologia , Salmonella typhi/imunologia , Formação de Anticorpos , Ensaio de Imunoadsorção Enzimática , Temperatura Alta , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Lipopolissacarídeos/imunologia , Fenol , Fenóis/farmacologiaRESUMO
Cross-reactive antibody responses were assessed in volunteers vaccinated with classical Inaba and Ogawa cholera vaccines. The El Tor, Ogawa vibrios, the most often biotype, and serotype found to be the causative agent of cholera in Thailand, or their product were used throughout the in vitro and in vivo tests. The test involved were the passive hemagglutination test, vibriocidal tests and the mouse protection test. Classes of specific immunoglobulins produced in the volunteers were determined using anti-immunoglobulin enhancement of hemagglutination. It was found that the levels of hemagglutinating and vibriocidal antibodies reached their peaks on day 7 after the vaccination and were statistically constant for 3 months. Significant decrease was observed thereafter. The mouse protective antibody titer was highest at 1 month after the vaccination then declined significantly at the 6th month. Classes of specific immunoglobulins were found to be either IgM or IgG alone or mixture of both.
Assuntos
Anticorpos Antibacterianos/biossíntese , Vacinas contra Cólera/imunologia , Cólera/prevenção & controle , Vibrio cholerae/imunologia , Animais , Cólera/imunologia , Testes de Hemaglutinação , Imunoglobulina G/biossíntese , Imunoglobulina M/biossíntese , Masculino , Camundongos , Prisioneiros , Tailândia , Vacinas Atenuadas/imunologiaRESUMO
This study was conducted on 506 firemen in Singapore. Interviews, pulmonary function tests and audiometry were conducted. With regard to pulmonary function, the results showed that forced vital capacity (FVC) increased up to the age of 25-30 years for both Chinese and Malays. Both FVC and forced expiratory volume in one second (FEV1.0) increased with standing height over the whole age range studied. The mean values of FVC and FEV1.0 were higher in Chinese. It was also found that the FEV1 of the subjects in the study showed a greater decline in rate with age than other workers studied by the authors previously. The hearing threshold of 83 fire fighters showed a prominent upward shift of 6-8 KHz at ages 20-30. This upward shift was more pronounced in the right ear. The implications of the findings are discussed and a comparison with results of other similar studies in other countries is made.