Immunostimulatory and antioxidant activities of the selenized polysaccharide from edible Grifola frondosa.

Grifola frondosa polysaccharide (GFP2) was extracted and purified by anion-exchange chromatography. A selenized G. frondosa polysaccharide, SeGFP2, was modified in selenylation by nitric acid-sodium selenite (HNO3-Na2SeO3) method. Structural features were investigated, and the lymphocyte proliferation and antioxidant activities were compared taking GFP2 as control. SeGFP2 with a molecular weight of 2.12 × 104 Da was composed of mannose, glucose, and galactose with a ratio of 3.5:11.8:1.0. A typical absorption of selenium ester was observed in SeGFP2 molecule. SeGFP2 was proposed as a branched polysaccharide, which consisted of 1,3-D-Glcp, 1,6-D-Glcp, 1,4,6-D-Galp, and 1,3,6-D-Manp. SeGFP2 showed a linear filamentous structure with some branches. SeGFP2 could significantly promote T- or B-lymphocyte proliferation and the enhancement was higher than GFP2. The in vitro antioxidant activities of SeGFP2 were more potent than GFP2. These present data suggested that selenylation could significantly improve the lymphocyte proliferation and in vitro antioxidant activities of GFP2.

Efficient and very selective dual mode chemosensor for Cd(II) ions in live cells.

A sensitive dual mode chemosensor NS-1 comprising Nitrobenzoxadiazole and salicylhydrazide conjugate has been synthesized via single step reaction. The probe NS-1 is characterized by analytical techniques such as multi nuclear NMR techniques and Mass spectrometry. The probe is showing a strong change in color from yellow to red on treatment of Cd(II) ions, interestingly its shows bright "Switch-ON" fluorescence state upon binding of Cd2+ ions in buffer solution whereas other cations did not showed any color change as well as fluorescent change. Interestingly the probe NS-1 did not results in the any color and fluorescence change with the adding together of Zn(II) ions, hence the probe is able to differentiate between Cd(II) ions from Zn(II). Further the color change and turn-on fluorescence can be rationalized by the interruption of internal charge transfer upon binding of Cd(II) ions with NS-1. The Internal charge transfer disturbance led to fluorescence change of the receptor NS-1 upon addition of Cd2+ has been further supported by TD-DFT calculations. The limit of detection was found to be 6.31 nano molar. The association constant was found to be 7.97*104 M-1 using Benesi-Hildebrand plot method. MTT assay suggesting that the probe NS-1 is least toxic to cells and it will be widely applicable to image Cd(II) ions in living cells via fluorescence imaging.

Gas Chromatography-Ion Mobility Spectrometry Detection of Odor Fingerprint as Markers of Rapeseed Oil Refined Grade.

In this work, gas chromatography-ion mobility spectrometry (GC-IMS) was used to analyze the volatile organic compound changes of rapeseed oil with different refined grades, the odor fingerprints of refined rapeseed oil were constructed, and a nonlinear model was built to realize rapid and accurate discrimination of rapeseed oil with different refined grades. 124 rapeseed oil samples with different refined grades were collected and analyzed by GC-IMS and chemometric tools, and 34 characteristic peaks were selected by the colorized difference method as variables to characterize the internal quality in rapeseed oil of different refined grades. The principal component analysis algorithm was used to further reduce dimensionality and extract the most relevant information. The k-nearest neighbor algorithm was applied to build a discriminant model. All the samples were recognized accurately without errors, and the results show the potential of this method to discriminate different refined grades of vegetable oil.