RESUMO
The distribution of LETS protein in human oral mucosa was studied by indirect immunofluorescence. Normal epithelium showed surface staining. Intracellular staining occurred in epithelial cell cytoplasm in lichen planus and pemphigoid. Surface staining was absent in discoid lupus erythematosus. In pemphigus, intercellular staining was seen near areas of acantholysis.
Assuntos
Glicoproteínas/metabolismo , Proteínas de Membrana/metabolismo , Doenças da Boca/metabolismo , Mucosa Bucal/metabolismo , Acantólise/patologia , Tecido Conjuntivo/metabolismo , Tecido Conjuntivo/patologia , Epitélio/metabolismo , Epitélio/patologia , Humanos , Líquen Plano/metabolismo , Líquen Plano/patologia , Lúpus Eritematoso Discoide/metabolismo , Lúpus Eritematoso Discoide/patologia , Doenças da Boca/patologia , Mucosa Bucal/anatomia & histologia , Pênfigo/metabolismo , Pênfigo/patologia , Dermatopatias Vesiculobolhosas/metabolismo , Dermatopatias Vesiculobolhosas/patologiaRESUMO
We retrospectively analyzed direct immunofluorescence (DIF) findings from 130 cases of oral mucosal disease. The diagnosis of each case was based on history, clinical features, histopathology, and clinical follow-up. To avoid circular reasoning, we did not use the DIF results in forming the diagnoses. Our results indicate that the presence of characteristic fluorescent patterns produced by several DIF reagents can establish the diagnosis of the oral lesions of pemphigus and pemphigoid and strongly indicate the diagnoses of lichen planus and lupus erythematosus. The absence of these fluorescent patterns can help to rule out these conditions, thereby strengthening the diagnoses of other oral mucosal diseases. The results of DIF are sufficiently distinguishing to be routinely helpful as diagnostic criteria for chronic ulcerative diseases of the oral mucosa.