RESUMO
Superficial fungal infections are common in dermatology and are often caused by opportunistic species in the Candida and Malassezia genera. The aim of this study was to analyze changes in the expression of genes coding for enzymes involved in the biosynthesis of glycosaminoglycans (GAGs) chains following the adherence of Candida and Malassezia yeasts to skin cell lines. Gene expression was analyzed using reverse transcriptase-quantitative polymerase chain reaction assays. Interactions between the yeasts and the skin cells induced the following changes in genes involved in the biosynthesis of heparan sulfate and chondroitin sulfate: downregulation of CHPF in keratinocytes and downregulation of EXT1, EXT2, CHSY3, and CHPF in fibroblasts. Adherence to fibroblasts had an even greater effect on GAG biosynthetic enzymes, inducing the downregulation of 13 genes and the upregulation of two (CHST15 and CHST7). Interactions between yeasts and skin cells might affect the binding affinity of GAG chains, possibly changing their ability to function as receptors for pathogens and interfering with a key stage at the start of infection.
Assuntos
Sulfatos de Condroitina , Malassezia , Candida albicans/genética , Candida albicans/metabolismo , Sulfatos de Condroitina/análise , Glicosaminoglicanos/análise , Glicosaminoglicanos/metabolismo , Heparitina Sulfato/análise , Heparitina Sulfato/metabolismo , Humanos , Malassezia/genética , Malassezia/metabolismo , Glicoproteínas de Membrana , SulfotransferasesRESUMO
Ionic channels play key roles in the sensory cells, such as transducing specific stimuli into electrical signals. The acid-sensing ion channel (ASIC) family is voltage-insensitive, amiloride-sensitive, proton-gated cation channels involved in several sensory functions. ASIC2, in particular, has a dual function as mechano- and chemo-sensor. In this study, we explored the possible role of zebrafish ASIC2 in olfaction. RT-PCR, Western blot, chromogenic in situ hybridization and immunohistochemistry, as well as ultrastructural analysis, were performed on the olfactory rosette of adult zebrafish. ASIC2 mRNA and protein were detected in homogenates of olfactory rosettes. Specific ASIC2 hybridization was observed in the luminal pole of the non-sensory epithelium, especially in the cilia basal bodies, and immunoreactivity for ASIC2 was restricted to the cilia of the non-sensory cells where it was co-localized with the cilia marker tubulin. ASIC2 expression was always absent in the olfactory cells. These findings demonstrate for the first time the expression of ASIC2 in the olfactory epithelium of adult zebrafish and suggest that it is not involved in olfaction. Since the cilium sense and transduce mechanical and chemical stimuli, ASIC2 expression in this location might be related to detection of aquatic environment pH variations or to detection of water movement through the nasal cavity.
Assuntos
Canais Iônicos Sensíveis a Ácido/metabolismo , Cílios/metabolismo , Epitélio/metabolismo , Mucosa Olfatória/citologia , Mucosa Olfatória/metabolismo , Canais Iônicos Sensíveis a Ácido/genética , Animais , Concentração de Íons de Hidrogênio , Peixe-ZebraRESUMO
Acid-sensing ion channels (ASICs) are H(+)-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs.
Assuntos
Canais Iônicos Sensíveis a Ácido/biossíntese , Proteínas do Olho/biossíntese , Regulação da Expressão Gênica/fisiologia , Retina/metabolismo , Proteínas de Peixe-Zebra/biossíntese , Peixe-Zebra/metabolismo , Animais , Retina/citologiaRESUMO
BACKGROUND AND OBJECTIVE: Superficial mycoses are some of the most common diseases worldwide. The usual culprits-yeasts belonging to the genera Malassezia and Candida-are commensal species in the skin that can cause opportunistic infections. We aimed to determine whether these yeasts use glycosaminoglycans (GAGs) as adhesion receptors to mediate binding to epithelial cells. MATERIAL AND METHODS: In keratinocyte and dermal fibroblast cultures, we used rhodamine B and genistein to inhibit GAG synthesis to study the role these molecules play in the adhesion of Candida albicans and Malassezia species to cells. We also analyzed GAG involvement by means of enzyme digestion, using specific lyases. RESULTS: Rhodamine B partially inhibited the adhesion of both fungi to keratinocytes but not to fibroblasts. Selective digestion of heparan sulfate enhanced the binding of Malassezia species to keratinocytes and of both fungi to fibroblasts. Chondroitin sulfate digestion decreased Calbicans adhesion to keratinocytes, but increased the adhesion of the filamentous forms of this species to fibroblasts. CONCLUSIONS: Cell surface GAGs appear to play a role in the adhesion of Calbicans and Malasezzia species to keratinocytes. In contrast, their adhesion to fibroblasts appears to be enhanced by GAG inhibition, suggesting that some other type of receptor is the mediator.
RESUMO
BACKGROUND AND OBJECTIVE: Superficial mycoses are some of the most common diseases worldwide. The usual culprits - yeasts belonging to the genera Malassezia and Candida - are commensal species in the skin that can cause opportunistic infections. We aimed to determine whether these yeasts use glycosaminoglycans (GAGs) as adhesion receptors to mediate binding to epithelial cells. MATERIAL AND METHODS: In keratinocyte and dermal fibroblast cultures, we used rhodamine B and genistein to inhibit GAG synthesis to study the role these molecules play in the adhesion of Candida albicans (C. albicans) and Malassezia species to cells. We also analyzed GAG involvement by means of enzyme digestion, using specific lyases. RESULTS: Rhodamine B partially inhibited the adhesion of both fungi to keratinocytes but not to fibroblasts. Selective digestion of heparan sulfate enhanced the binding of Malassezia species to keratinocytes and of both fungi to fibroblasts. Chondroitin sulfate digestion decreased C. albicans adhesion to keratinocytes, but increased the adhesion of the filamentous forms of this species to fibroblasts. CONCLUSIONS: Cell surface GAGs appear to play a role in the adhesion of C albicans and Malasezzia species to keratinocytes. In contrast, their adhesion to fibroblasts appears to be enhanced by GAG inhibition, suggesting that some other type of receptor is the mediator.
RESUMO
Specific adherence is the first requisite that a microorganism has to fulfil to become established onto a mucosal surface. It was previously shown that the OppA surface protein of Lactobacillus salivarius Lv72 bound HeLa cell cultures through interaction with glycosaminoglycans (GAGs). To determine whether this is a peculiarity of that strain or whether it can be extended to other lactobacilli, 12 strains, belonging to six species, were confronted with HeLa-cell cultures in the presence of soluble GAGs. Interference was observed to six of them, heparan sulphate and chondroitin sulphate C being more interfering than chondroitin sulphate A or chondroitin sulphate B. Furthermore, inhibition of the biosynthesis of GAGs or their elimination from the cell surface with specific enzymes also resulted in reduced adherence. Analysis of the surface proteome of Lactobacillus crispatus Lv25 and of Lactobacillus reuteri RC14 revealed single proteins that immunoreacted with antibodies raised against OppA, the main adhesin of L. salivarius Lv72. Upon MALDI-TOF-TOF analysis, they were identified as OppA-like proteins, thus indicating that these proteins participate as adhesins in attachment of diverse lactobacilli to the surface of human epithelial cells.
Assuntos
Adesinas Bacterianas/metabolismo , Células Epiteliais/metabolismo , Glicosaminoglicanos/metabolismo , Lactobacillus/metabolismo , Adesinas Bacterianas/química , Motivos de Aminoácidos , Aderência Bacteriana/efeitos dos fármacos , Glicosaminoglicanos/antagonistas & inibidores , Glicosaminoglicanos/farmacologia , Células HeLa , Humanos , Lactobacillus/genética , Proteoma/química , Proteoma/metabolismo , Rodaminas/farmacologiaRESUMO
Glycosaminoglycans are involved in the attachment of Lactobacillus salivarius Lv72, a strain of vaginal origin, to HeLa cell cultures, indicating that they play a fundamental role in the attachment of mutualistic bacteria to the epithelium lining cavities where the normal microbiota thrives. The bacterial OppA protein has been proposed as an adhesin involved in this adherence since, once purified, it significantly interferes with attachment of the lactobacilli to HeLa cell cultures. In this article, the role of OppA is confirmed through the determination of its location at the cell surface and its ability to promote Lactobacillus casei and Lactococcus lactis adherence to eukaryotic cell cultures upon cloning and expression of oppA in these bacteria. The OppA sequence showed five potential domains for glycosaminoglycan-binding, and structural modelling of the protein showed that two of them were located in the vicinity of an OppA superficial groove whose width approached the diameter of the helical form of heparin in solution. Their involvement in the binding was demonstrated through substitution of critical basic amino acids by acidic ones, which resulted in loss of affinity for heparan sulphate and chondroitin sulphate depending on the domain mutated, suggesting that there might be a certain degree of specialisation. In addition, circular dichroism analysis showed that the spectrum changes induced by OppA-heparan sulphate binding were attenuated by the variant proteins, indicating that these motifs are the OppA recognition domains for the eukaryotic cell surface.
Assuntos
Adesinas Bacterianas/química , Adesinas Bacterianas/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Ligilactobacillus salivarius/fisiologia , Lipoproteínas/química , Lipoproteínas/metabolismo , Adesinas Bacterianas/genética , Motivos de Aminoácidos , Aderência Bacteriana , Proteínas de Bactérias/genética , Proteínas de Transporte/genética , Glicosaminoglicanos/metabolismo , Células HeLa , Humanos , Lipoproteínas/genéticaRESUMO
Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule). The cells forming those compartments are continuous and share the properties of that covering the nerve fibers. Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly. Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles. The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican. Asporin, prolargin and keratocan were undetectable. These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
Assuntos
Corpúsculos de Pacini/química , Proteoglicanas/análise , Adolescente , Adulto , Animais , Antígenos CD34/análise , Biglicano/análise , Criança , Decorina/análise , Equidae , Proteínas da Matriz Extracelular/análise , Fibromodulina/análise , Dedos , Imunofluorescência , Cabras , Humanos , Imuno-Histoquímica , Camundongos , Pessoa de Meia-Idade , Proteoglicanas/classificação , Coelhos , Proteínas S100/análise , Pele/anatomia & histologia , Vimentina/análise , Adulto JovemRESUMO
BACKGROUND: Mithramycin is a member of the clinically important aureolic acid group of antitumor drugs that interact with GC-rich regions of DNA nonintercalatively. These drugs contain a chromophore aglycon that is derived from condensation of ten acetate units (catalyzed by a type II polyketide synthase). The aglycones are glycosylated at two positions with different chain length deoxyoligosaccharides, which are essential for the antitumor activity. During the early stages of mithramycin biosynthesis, tetracyclic intermediates of the tetracycline-type occur, which must be converted at later stages into the tricyclic glycosylated molecule, presumably through oxidative breakage of the fourth ring. RESULTS: Two intermediates in the mithramycin biosynthetic pathway, 4-demethyl-premithramycinone and premithramycin B, were identified in a mutant lacking the mithramycin glycosyltransferase and methyltransferase genes and in the same mutant complemented with the deleted genes, respectively. Premithramycin B contains five deoxysugars moieties (like mithramycin), but contains a tetracyclic aglycon moiety instead of a tricyclic aglycon. We hypothesized that transcription of mtmOIV (encoding an oxygenase) was impaired in this strain, preventing oxidative breakage of the fourth ring of premithramycin B. Inactivating mtmOIV generated a mithramycin nonproducing mutant that accumulated premithramycin B instead of mithramycin. In vitro assays demonstrated that MtmOIV converted premithramycin B into a tricyclic compound. CONCLUSIONS: In the late stages of mithramycin biosynthesis by Strepyomyces argillaceus, a fully glycosylated tetracyclic tetracycline-like intermediate (premithramycin B) is converted into a tricyclic compound by the oxygenase MtmOIV. This oxygenase inserts an oxygen (Baeyer-Villiger oxidation) and opens the resulting lactone. The following decarboxylation and ketoreduction steps lead to mithramycin. Opening of the fourth ring represents one of the last steps in mithramycin biosynthesis.
Assuntos
Antibióticos Antineoplásicos/biossíntese , Oxigenases/genética , Plicamicina/biossíntese , Streptomyces/metabolismo , Sequência de Aminoácidos , Antibióticos Antineoplásicos/metabolismo , Sequência de Carboidratos , Catálise , Glicosiltransferases/metabolismo , Espectroscopia de Ressonância Magnética , Metiltransferases/metabolismo , Dados de Sequência Molecular , Complexos Multienzimáticos/metabolismo , Mutagênese Insercional , Mutação , Oxirredução , Oxigenases/metabolismo , Plicamicina/metabolismo , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Homologia de Sequência de Aminoácidos , Streptomyces/genéticaRESUMO
Macrolides are a group of antibiotics structurally characterized by a macrocyclic lactone to which one or several deoxy-sugar moieties are attached. The sugar moieties are transferred to the different aglycones by glycosyltransferases (GTF). The OleI GTF of an oleandomycin producer, Streptomyces antibioticus, catalyzes the inactivation of this macrolide by glycosylation. The product of this reaction was isolated and its structure elucidated. The donor substrate of the reaction was UDP-alpha-D-glucose, but the reaction product showed a beta-glycosidic linkage. The inversion of the anomeric configuration of the transferred sugar and other data about the kinetics of the reaction and primary structure analysis of several GTFs are compatible with a reaction mechanism involving a single nucleophilic substitution at the sugar anomeric carbon in the catalytic center of the enzyme.
Assuntos
Antibacterianos/antagonistas & inibidores , Antibacterianos/química , Glucosiltransferases/metabolismo , Oleandomicina/antagonistas & inibidores , Oleandomicina/química , Sequência de Aminoácidos , Antibacterianos/metabolismo , Configuração de Carboidratos , Glucose/química , Glucosiltransferases/genética , Glicosilação , Espectroscopia de Ressonância Magnética , Modelos Químicos , Oleandomicina/metabolismo , Homologia de Sequência de Aminoácidos , Streptomyces antibioticus/enzimologia , Streptomyces antibioticus/genéticaRESUMO
A synthetic peptide (23 residues) that includes the antibacterial and lipopolysaccharide-binding regions of human lactoferricin, an antimicrobial sequence of lactoferrin, was used to study its action on cytoplasmic membrane of Escherichia coli 0111 and E. coli phospholipid vesicles. The peptide caused a depolarization of the bacterial cytoplasmic membrane, loss of the pH gradient, and a bactericidal effect on E. coli. Similarly, the binding of the peptide to liposomes dissipated previously created transmembrane electrical and pH gradients. The dramatic consequences of the transmembrane ion flux during the peptide exposure indicate that the adverse effect on bacterial cells occurs at the bacterial inner membrane.
Assuntos
Lactoferrina/análogos & derivados , Lactoferrina/farmacologia , Membranas Artificiais , Antibacterianos/farmacologia , Eletrofisiologia , Escherichia coli/química , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/metabolismo , Humanos , Concentração de Íons de Hidrogênio/efeitos dos fármacos , Lipossomos/efeitos dos fármacos , Lipossomos/metabolismo , Potenciais da Membrana/efeitos dos fármacos , Permeabilidade/efeitos dos fármacos , Ligação Proteica/efeitos dos fármacos , Espectrometria de Fluorescência , Fatores de Tempo , Triptofano/metabolismo , Valinomicina/farmacologiaRESUMO
Human lactoferrin was bactericidal in vitro for Micrococcus luteus but not for other Micrococcus species (M. radiophilus, M. roseus and M. varians). A correlation between the binding of lactoferrin to the bacterial surface and the antimicrobial action was observed. Viability assays showed that ferric, but not ferrous, salts prevented binding and consequently M. luteus was not killed. The unsaturated form of lactoferrin showed a greater affinity than that of the iron-saturated molecule for lipomannan, a lipoglycan present on the cell wall of M. luteus, supporting the role for lipomannan as one of the possible binding sites of lactoferrin on M. luteus.
Assuntos
Antibacterianos/farmacologia , Lactoferrina/farmacologia , Micrococcus luteus/efeitos dos fármacos , Antibacterianos/metabolismo , Apoproteínas/metabolismo , Compostos Férricos/farmacologia , Humanos , Lactoferrina/metabolismo , Lipopolissacarídeos/metabolismo , Micrococcus/efeitos dos fármacos , Micrococcus luteus/crescimento & desenvolvimentoRESUMO
A beta-lactamase in oral clinical isolates of Prevotella intermedia that hydrolyzed cefuroxime and cephalothin with rates of 600 and 53.3 respectively, relative to that for cephaloridine (100), was characterized as a 2e-cephalosporinase. Inhibition was observed by clavulanic acid (IC50 0.72 microM), tazobactam (IC50 0.21 microM) and sulbactam (IC50 0.07 microM) and was not inhibited by cloxacillin, EDTA, NaCl or p-CMB. The pI and pH optima were 4.7 and 5.4, respectively.
Assuntos
Infecções por Bacteroidaceae/microbiologia , Prevotella intermedia/enzimologia , beta-Lactamases/isolamento & purificação , Antibacterianos/farmacologia , Cefalosporinas/metabolismo , Humanos , Cinética , Periodontite/microbiologia , Inibidores de beta-Lactamases , beta-Lactamases/metabolismoRESUMO
The oleandomycin (OM) producer, Streptomyces antibioticus, possesses a mechanism involving two enzymes for the intracellular inactivation and extracellular reactivation of the antibiotic. Inactivation takes place by transfer of a glucose molecule from a donor (UDP-glucose) to OM, a process catalyzed by an intracellular glucosyltransferase. Glucosyltransferase activity is detectable in cell-free extracts concurrent with biosynthesis of OM. The enzyme has been purified 1,097-fold as a monomer, with a molecular mass of 57.1 kDa by a four-step procedure using three chromatographic columns. The reaction operates via a compulsory-order mechanism. This has been shown by steady-state kinetic studies using either OM or an alternative substrate (rosaramycin) and dead-end inhibitors, and isotopic exchange reactions at equilibrium. OM binds first to the enzyme, followed by UDP-glucose. A ternary complex is thus formed prior to transfer of glucose. UDP is then released, followed by the glycosylated oleandomycin (GS-OM).
Assuntos
Glucosiltransferases/isolamento & purificação , Glucosiltransferases/metabolismo , Oleandomicina/biossíntese , Streptomyces antibioticus/metabolismo , Eritromicina/farmacologia , Glicosilação , Cinética , Streptomyces antibioticus/enzimologia , Uridina Difosfato Glucose/farmacologiaRESUMO
The structure and functionality of the ribosomal subunits of the substrate and the aerial mycelium of Streptomyces antibioticus were compared. Using SDS-PAGE and HPLC, several differences between the ribosomal protein pattern from both stages of development were observed, including a clear decrease in the L7/L12 content of the aerial mycelium. The activity of the aerial mycelia ribosomes was also decreased when compared with that of the substrate mycelium. This effect was more pronounced in the 50S subunit. These results suggest that during cell differentiation in Streptomyces important changes occur at the ribosomal level, particularly in the transition from the substrate to the aerial mycelium.
Assuntos
Proteínas de Bactérias/metabolismo , Proteínas Ribossômicas/metabolismo , Streptomyces/metabolismo , Regulação Bacteriana da Expressão Gênica , Esporos Bacterianos , Streptomyces/crescimento & desenvolvimento , Streptomyces/fisiologiaRESUMO
A simple procedure for the isolation of membranes from Streptomyces spores is described which produces about 12 mg of membrane protein per g of dry weight. The membrane fractions were contaminated by low levels of DNA, RNA, and hexosamines. The functional integrity of the membrane is conserved through the isolation procedure, as evaluated by the presence of several activities of the membrane-bound electron transport chain. This isolation procedure allowed the determination of the biosynthesis of proteins and phospholipids of the membrane. Both biosynthetic processes started in the first 5 min of germination and increased progressively during spore germination. A stable mRNA fraction of the dormant spore encoded 44% of the membrane proteins synthesized early in germination, but most of the phospholipid biosynthesis was not dependent on this fraction.
Assuntos
Esporos Bacterianos/ultraestrutura , Streptomyces antibioticus/ultraestrutura , Streptomyces/ultraestrutura , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Fracionamento Celular , Proteínas de Membrana/biossíntese , Proteínas de Membrana/genética , Membranas/análise , Membranas/metabolismo , Fosfolipídeos/biossíntese , RNA Bacteriano/genética , RNA Mensageiro/genética , Esporos Bacterianos/metabolismo , Streptomyces antibioticus/genética , Streptomyces antibioticus/metabolismo , Streptomyces antibioticus/fisiologiaRESUMO
Cell-free extracts from the oleandomycin producer, Streptomyces antibioticus, possess an intracellular glycosyltransferase capable of inactivating oleandomycin by glycosylation of the 2'-hydroxyl group in the desosamine moiety of the molecule [Vilches, C., Hernández, C., Méndez, C. & Salas, J. A. (1992) J. Bacteriol. 174, 161-165]. Using a four-step purification procedure, we have purified an enzyme activity from the culture supernatants from this organism which is able to release glucose from the inactive glycosylated molecule thus reactivating the antibiotic activity. This enzyme activity appeared in the culture supernatants immediately before oleandomycin is detected. The enzyme (molecular mass 87 kDa) showed a high degree of substrate specificity, not acting on other glycosylated macrolides such as methymycin, lankamycin and rosaramicin which are substrates for the glycosyltransferase. A second activity was detected corresponding to a 34-kDa polypeptide which probably originates from proteolytic cleavage of the larger polypeptide. The 87-kDa polypeptide possibly catalyses the last biosynthetic step in oleandomycin biosynthesis by S. antibioticus.
Assuntos
Enzimas/isolamento & purificação , Oleandomicina/metabolismo , Streptomyces antibioticus/enzimologia , Biotransformação , Cromatografia por Troca Iônica , Eletroforese em Papel , Eletroforese em Gel de Poliacrilamida , Ativação Enzimática , Enzimas/metabolismo , Glicosilação , Cinética , Oleandomicina/biossíntese , Especificidade por SubstratoRESUMO
Clinical isolates of Staphylococcus and Arthrobacter spp. were screened for lincosamide resistance. Six different patterns of resistance were found. Strains designated SF27 and SF28 showed low-level resistance to lincosamides: one was susceptible to erythromycin (SF27) and the other was resistant (SF28). Analysis of ribosomes from the resistant strains in an in vitro poly(U)-dependent protein-synthesizing system showed that ribosomes of both strains were sensitive to lincomycin and clindamycin. Four patterns of high-level resistance to lincosamides were observed (strains SF4, SF19, SF30, and SF31). All of these except SF30 had ribosomes which were highly resistant in vitro to the antibiotics and showed a close correlation with results of the in vivo experiments. In vivo protein synthesis by strain SF30 was resistant to lincomycin and sensitive to clindamycin, whereas the ribosomes were sensitive when assayed in vitro. Lincosamide-inactivating enzymes were not detected in cell extracts of the six resistant strains. Strains SF19 and SF31 demonstrated two ribosome-mediated lincosamides resistance mechanisms that were not previously reported. Both strains were highly resistant to lincosamides and susceptible to erythromycin, but SF19 was also highly resistant to oleandomycin and partially resistant to various macrolides.
Assuntos
Antibacterianos/farmacologia , Arthrobacter/efeitos dos fármacos , Staphylococcus/efeitos dos fármacos , Aminoglicosídeos , Proteínas de Bactérias/biossíntese , Clindamicina/farmacologia , Resistência Microbiana a Medicamentos , Eritromicina/farmacologia , Lincomicina/farmacologia , Testes de Sensibilidade Microbiana , Ribossomos/efeitos dos fármacosRESUMO
The oleD gene has been identified in the oleandomycin producer Streptomyces antibioticus and it codes a macrolide glycosyltransferase that is able to transfer a glucose moiety from UDP-glucose (UDP-Glc) to many macrolides. The glycosyltransferase coded by the oleD gene has been purified 371-fold from a Streptomyces lividans clone expressing this protein. The reaction product was isolated, and its structure determined by NMR spectroscopy. The kinetic mechanism of the reaction was analyzed using the macrolide antibiotic lankamycin (LK) as substrate. The reaction operates via a compulsory order mechanism. This has been shown by steady-state kinetic studies and by isotopic exchange reactions at equilibrium. LK binds first to the enzyme, followed by UDP-glucose. A ternary complex is thus formed prior to transfer of glucose. UDP is then released, followed by the glycosylated lankamycin (GS-LK). A pH study of the reaction was performed to determine values for the molecular pK values, suggesting possible amino acid residues involved in the catalytic process.
Assuntos
Antibacterianos/metabolismo , Glucosiltransferases/isolamento & purificação , Glucosiltransferases/metabolismo , Streptomyces antibioticus/enzimologia , Sequência de Aminoácidos , Catálise , Eritromicina/análogos & derivados , Eritromicina/metabolismo , Glicosilação , Concentração de Íons de Hidrogênio , Cinética , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Oleandomicina/metabolismo , Dobramento de Proteína , Difosfato de Uridina/metabolismo , Uridina Difosfato Glucose/metabolismoRESUMO
A 5.2 kb region from the oleandomycin gene cluster in Streptomyces antibioticus located between the oleandomycin polyketide synthase gene and sugar biosynthetic genes was cloned. Sequence analysis revealed the presence of three open reading frames (designated oleI, oleN2 and oleR). The oleI gene product resembled glycosyltransferases involved in macrolide inactivation including the oleD product, a previously described glycosyltransferase from S. antibioticus. The oleN2 gene product showed similarities with different aminotransferases involved in the biosynthesis of 6-deoxyhexoses. The oleR gene product was similar to several glucosidases from different origins. The oleI, oleR and oleD genes were expressed in Streptomyces lividans. OleI and OleD intracellular proteins were partially purified by affinity chromatography in an UDP-glucuronic acid agarose column and OleR was detected as a major band from the culture supernatant. OleI and OleD showed oleandomycin glycosylating activity but they differ in the pattern of substrate specificity: OleI being much more specific for oleandomycin. OleR showed glycosidase activity converting glycosylated oleandomycin into active oleandomycin. A model is proposed integrating these and previously reported results for intracellular inactivation, secretion and extracellular reactivation of oleandomycin.