RESUMO
Glycogen synthase kinase-3 (GSK-3) is a constitutively active kinase, involved in regulation of multiple physiological processes. In brain, changes in GSK-3 signaling are related to neurodegenerative issues, including Alzheimer's disease. Due to the wide range of GSK-3 cellular targets, a therapeutic use of the enzyme inhibitors entails significant risk of side effects. Thus, altering the ratio of specific pool of GSK-3 or specific substrates instead of changing the global activity of GSK-3 in brains might be a more appropriate strategy. This paper provides a comprehensive data on abundances of proteins involved in GSK-3 signaling in three regions of young and old mouse brains. It might help to identify novel protein targets with the highest therapeutic potential for treatment of age-related neurodegenerative diseases.
Assuntos
Envelhecimento/fisiologia , Encéfalo/metabolismo , Cerebelo/metabolismo , Quinase 3 da Glicogênio Sintase/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Hipocampo/metabolismo , Camundongos , Proteoma/metabolismo , Transdução de Sinais/efeitos dos fármacosRESUMO
Adenosine 5'-monophosphate (AMP) inhibits muscle fructose 1,6-bisphosphatase (FBPase) about 44 times stronger than the liver isozyme. The key role in strong AMP binding to muscle isozyme play K20, T177 and Q179. Muscle FBPase which has been mutated towards the liver enzyme (K20E/T177M/Q179C) is inhibited by AMP about 26 times weaker than the wild-type muscle enzyme, but it binds the fluorescent AMP analogue, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-monophosphate (TNP-AMP), similarly to the wild-type liver enzyme. The reverse mutation of liver FBPase towards the muscle isozyme significantly increases the affinity of the mutant to TNP-AMP. High affinity to the inhibitor but low sensitivity to AMP of the liver triple mutant suggest differences between the isozymes in the mechanism of allosteric signal transmission.
Assuntos
Monofosfato de Adenosina/farmacologia , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfatase/genética , Músculos/enzimologia , Monofosfato de Adenosina/análogos & derivados , Regulação Alostérica/genética , Sequência de Aminoácidos , Aminoácidos/genética , Humanos , Isoenzimas/antagonistas & inibidores , Isoenzimas/genética , Fígado/enzimologia , Dados de Sequência Molecular , MutaçãoRESUMO
Fructose 1,6-bisphosphatase (FBPase)--a key enzyme of gluconeogenesis--for a long time was regarded to be soluble, and freely diffused in the cytoplasm. Our recent investigation revealed however, that in skeletal muscles of mammals, FBPase is located on both sides of the Z-line and, in cardiomyocytes, it is also present inside the cells' nuclei. In the current paper we demonstrate that, in smooth muscle cells, FBPase is located in the cytoplasm and the nucleus, and that the presence of the enzyme in the nucleus is almost completely restricted to the heterochromatin area. In search for additional evidence for the nuclear localization of FBPase and for a possible explanation of its role in the nucleus, we have analyzed the primary structures of muscle FBPases, finding on their molecular surface a number of domains specific for proteins transported into the nucleus.
Assuntos
Núcleo Celular/enzimologia , Frutose-Bifosfatase/metabolismo , Miócitos de Músculo Liso/enzimologia , Animais , Sítios de Ligação , Núcleo Celular/ultraestrutura , Frutose-Bifosfatase/química , Miócitos de Músculo Liso/citologia , Miócitos de Músculo Liso/ultraestrutura , Estrutura Quaternária de Proteína , Transporte Proteico , Coelhos , Frações Subcelulares/enzimologia , SuínosRESUMO
Real-time interaction analysis, using the BIAcore biosensor, of rabbit muscle FBPase-aldolase complex revealed apparent binding constant [K(Aapp)] values of about 4.4x10(8) M(-1). The stability of the complex was down-regulated by the glycolytic intermediates dihydroxyacetone phosphate and fructose 6-phosphate, and by the regulator of glycolysis and glyconeogenesis--fructose 2,6-bisphosphate. FBPase in a complex with aldolase was entirely insensitive to inhibition by physiological concentrations of AMP (I(0.5) was 1.35 mM) and the cooperativity of the inhibition was not observed. The existence of an FBPase-aldolase complex that is insensitive to AMP inhibition explains the possibility of glycogen synthesis from carbohydrate precursors in vertebrates' myocytes.
Assuntos
Monofosfato de Adenosina/farmacologia , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfato Aldolase/antagonistas & inibidores , Músculo Esquelético/enzimologia , Animais , Fosfato de Di-Hidroxiacetona/farmacologia , Inibidores Enzimáticos/farmacologia , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Frutose-Bifosfato Aldolase/isolamento & purificação , Frutose-Bifosfato Aldolase/metabolismo , Gluconeogênese , Glicólise , Cinética , CoelhosRESUMO
The localization of fructose-1,6-bisphosphatase (FBPase) in human skeletal muscle was determined immunohistochemically using polyclonal antibodies. Light microscopy analysis, confirmed with the use of confocal microscopy, indicated that the enzyme is localized on both sides of the Z line of myocytes. The immunohistochemical investigation was confirmed by a co-sedimentation experiment which revealed that muscle FBPase binds strongly to alpha-actinin--a major structural protein of the Z line. This is the first report on localization of FBPase in skeletal muscle tissue.
Assuntos
Frutose-Bifosfatase/metabolismo , Músculo Esquelético/enzimologia , Actinina/metabolismo , Western Blotting , Humanos , Imuno-Histoquímica , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Células Musculares/enzimologia , Células Musculares/ultraestrutura , Músculo Esquelético/citologia , Ligação ProteicaRESUMO
The localization of fructose-1,6-bisphosphatase (Fru-1,6-Pase EC 3.1.3.11) in human alveolar epithelium was determined immunohistochemically using a polyclonal antibody raised against the enzyme purified from human liver. The immunohistochemical analysis revealed that the Fru-1,6-Pase was localized in pneumocytes II and was absent in pneumocytes I. Hypothetically Fru-1,6-Pase participating in glucose-6-phosphate synthesis from noncarbohydrate precursors increases NADPH level which is used for surfactant synthesis and for glutathione redox cycle.
Assuntos
Frutose-Bifosfatase/metabolismo , Pulmão/citologia , Pulmão/enzimologia , Animais , Frutose-Bifosfatase/imunologia , Humanos , Técnicas Imunoenzimáticas , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Inclusão em Parafina , Alvéolos Pulmonares/citologia , Alvéolos Pulmonares/enzimologia , Coelhos/imunologia , Fixação de TecidosRESUMO
F1,6BPases from porcine and bovine lung were isolated and their kinetic properties were determined. Ks, Kis and beta were determined assuming partial-noncompetitive inhibition (simple intersecting hyperbolic noncompetitive inhibition) of the enzyme by the substrate. Values for Ks were 4.1 and 4.4 microM for porcine and bovine F1,6BPase, respectively and values for 1 were close to 0.55 in both cases. Kis were 9 and 15 microM for porcine and bovine F1,6BPase, respectively. I0.5 for AMP were determined as 7 microM for pig enzyme and 14 microM for F1,6BPase from bovine lung. The enzymes were inhibited by F2,6BP with Ki's of 0.19 and 0.21 microM for porcine and bovine enzymes, respectively. In the presence of AMP concentration equal to I0.5, the Ki values for pig and bovine enzymes were 0.07 and 0.09 microM, respectively. The levels of F2,6BP, AMP and antioxidant enzymes activities in pig and bovine lung tissues were also determined. The cDNA coding sequence of pig lung F1,6BPase1 showed a high homology with pig liver enzyme, differing only in four positions (G/C-63, T/A-808, G/C-884 and T/A-1005) resulting in a single amino acid substitution (Gly-295 for Ala-295). It is hypothesized that the lung F1,6BPase participates in gluconeogenesis, surfactant synthesis and antioxidant reactions.
Assuntos
Frutose-Bifosfatase/química , Isoenzimas , Pulmão/enzimologia , Monofosfato de Adenosina/metabolismo , Animais , Catalase/metabolismo , Bovinos , DNA Complementar/metabolismo , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Frutose-Bifosfatase/fisiologia , Glucose-6-Fosfatase/metabolismo , Glucosefosfato Desidrogenase/metabolismo , Glutationa Redutase/metabolismo , Concentração Inibidora 50 , Cinética , Fígado/enzimologia , Magnésio/farmacologia , Modelos Biológicos , Modelos Químicos , Dados de Sequência Molecular , Oxirredutases/metabolismo , Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo , Fosfogluconato Desidrogenase/metabolismo , RNA Mensageiro/metabolismo , Especificidade por Substrato , Superóxido Dismutase/metabolismo , SuínosRESUMO
Muscle aldolase bound to muscle FBPase (K(d) = 8.7 microM) decreases the latter's sensitivity towards AMP inhibition. I(0.5) of muscle FBPase was increased from 0.06 microM to 0.65 microM when determined in the presence of 10 microM of muscle aldolase. In the presence of 10 microM of liver aldolase I(0.5) of liver FBPase was increased only twofold, from 11.0 microM to 21.7 microM. The effect of muscle aldolase on liver FBPase and liver aldolase on muscle FBPase is rather negligible. Aldolase slightly affected interaction of FBPase with magnesium ions decreasing K(a) and Hill constant (n). No effect of aldolase on FBPase pH optimum was observed.
Assuntos
Monofosfato de Adenosina/farmacologia , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfato Aldolase/metabolismo , Músculo Esquelético/enzimologia , Animais , Frutose-Bifosfatase/metabolismo , Cinética , Fígado/enzimologia , CoelhosRESUMO
Previously we have reported that in vitro muscle aldolase binds to muscle FBPase [Biochem. Biophys. Res. Commun. 275 (2000) 611-616] which results in the changes of regulatory properties of the latter enzyme. In the present paper, the evidence that aldolase binds to FBPase in living cell is presented. The colocalization experiment, in which aldolase was diffused into skinned fibres that had been pre-incubated with FBPase, has shown that aldolase in the presence of FBPase binds predominantly to the Z-line. The existence of a triple aldolase-FBPase-alpha-actinin complex was confirmed through a real-time interaction analysis using the BIAcore biosensor. The colocalization of FBPase and aldolase on alpha-actinin of the Z-line indicates the existence of glyconeogenic metabolon in vertebrates' myocytes.
Assuntos
Actinina/metabolismo , Frutose-Bifosfatase/metabolismo , Frutose-Bifosfato Aldolase/metabolismo , Fibras Musculares Esqueléticas/enzimologia , Músculos Psoas/enzimologia , Actinina/química , Actinina/ultraestrutura , Animais , Coenzimas/metabolismo , Coenzimas/ultraestrutura , Frutose-Bifosfatase/química , Frutose-Bifosfatase/ultraestrutura , Frutose-Bifosfato Aldolase/química , Frutose-Bifosfato Aldolase/ultraestrutura , Substâncias Macromoleculares , Fibras Musculares Esqueléticas/química , Fibras Musculares Esqueléticas/citologia , Ligação Proteica , Músculos Psoas/citologia , Coelhos , Distribuição TecidualRESUMO
A cDNA encoding fructose(1,6)bisphosphatase was isolated from total human lung RNA. The cDNA contained an open reading frame encoding 337 amino acids. The determined nucleotide sequence of the lung cDNA was significantly different from muscle cDNA and slightly differed from human liver cDNA in a single mutation (Gly-336 for Ala-336) and a T for C substitution in position 648. The human lung fructose(1, 6)bisphosphatase [Fru(1,6)Pase] was isolated and its kinetic parameters were compared with liver and muscle isoenzymes. Values of kcat for the lung Fru(1,6)Pase were lower than for the liver and muscle enzyme. Like the liver isoenzyme, lung Fru(1,6)Pase is significantly less inhibited by AMP than the muscle enzyme. The values of I0.5 were 9.5, 9.8, and 0.3 microM for the liver, lung, and muscle enzyme, respectively. The lung enzyme was slightly more sensitive to fructose(2,6)bisphosphate [Fru(2,6)P2] inhibition than the liver enzyme. Ki was 75 microM for the lung and 96 microM for the liver enzyme. The synergistic effect of AMP and Fru(2,6)P2 on the lung and liver Fru(1,6)Pase was also observed. In the presence of AMP the corresponding values of Ki for Fru(2,6)P2 were 16 microM for the lung and 10 microM for the liver enzyme.