Antihistamine and Wound Healing Potential of Gold Nanoparticles Synthesized Using Bulbine frutescens (L.) Willd.

Background: Atopic dermatitis (eczema) is an inflammatory skin condition with synthetic treatments that induce adverse effects and are ineffective. One of the proposed causes for the development of the condition is the outside-in hypothesis, which states that eczema is caused by a disruption in the skin barrier. These disruptions include developing dry cracked skin, which promotes the production of histamine. Bulbine frutescens (BF) is traditionally used to treat wounds and eczema; however, limited research has been conducted to scientifically validate this. Furthermore, gold nanoparticles (AuNPs) have been used to repair damaged skin; however, no research has been conducted on AuNPs synthesized using BF. Purpose: The study aimed to determine whether BF alleviated skin damage through wound healing, reducing the production of histamine and investigate whether AuNPs synthesized using BF would enhance biological activity. Methods: Four extracts and four synthesized AuNPs were prepared using BF and their antiproliferative and wound healing properties against human keratinocyte cells (HaCaT) were evaluated. Thereafter, the selected samples antiproliferative activity and antihistamine activity against phorbol 12-myristate 13-acetate (PMA) stimulated granulocytes were evaluated. Results: Of the eight samples, the freeze-dried leaf juice (BFE; p < 0.01) extract and its AuNPs (BFEAuNPs; p < 0.05) displayed significant wound closure at 100 µg/mL and were further evaluated. The selected samples displayed a fifty percent inhibitory concentration (IC50) of >200 µg/mL against PMA stimulated granulocytes. Compared to the untreated (media with PMA) control (0.30 ± 0.02 ng/mL), BFEAuNPs significantly inhibited histamine production at a concentration of 100 (p < 0.01) and 50 µg/mL (p < 0.001). Conclusion: BFE and BFEAuNPs stimulated wound closure, while BFEAuNPs significantly inhibited histamine production. Further investigation into BFEAuNPs in vivo wound healing activity and whether it can target histamine-associated receptors on mast cells as a potential mechanism of action should be considered.

Granular morphology, molecular structure and thermal stability of infrared heat-moisture treated maize starch with added lipids.

The thermal and structural changes of infrared heat-moisture treated (IR-HMT) maize starch with added stearic acid (SA) (1.5% w/w) were investigated. Scanning electron microscopy showed more protrusion and fewer pores on the starch granular surface for maize starch with IR-HMT plus SA. Transmission electron microscopy showed that IR-HMT promoted more interactions between starch blocklets and resulted in a more fused blocklet organisation. IR-HMT seemed to increase hydrogen bonding between starch polymeric chains. These interactions are shown by the increased relative crystallinity shown by crystalline peak area calculated from X-ray diffraction and the higher 1047/1022 cm-1 band ratio from FTIR at a molecular level. In addition, at a molecular level, dynamic mechanical thermal analysis and differential scanning calorimetry showed evidence of amylose-lipid complexes in IR-HMT starch with SA. These changes can be related to already reported functionality such as reduced starch digestibility of IR-HMT maize starch with stearic acid IR-HMT.

Structural and digestibility properties of infrared heat-moisture treated maize starch complexed with stearic acid.

This study investigated the effects of using infrared heat-moisture treatment (IRHMT) on the properties of maize starch paste complexed with stearic acid (SA). Scanning electron micrographs showed that starch granules ghosts from IR HMT starch with SA did not show significant granular disintegration in comparison to conventionally HMT starch paste. The resistant starch (RS) content increased with SA-IR HMT, while extended pasting increased slowly digestible starch (SDS) content in IR HMT starch alone. The V polymorphs observed in XRD and DSC, and increased crystallinity from FTIR supported the changes in the properties of IRHMT starches. To a greater extent, the SA-IRHMT exerted more changes on starch micro- and molecular structural properties, and digestibility properties compared to conventional heat-moisture treatment (CHMT).

Plastics in municipal drinking water and wastewater treatment plant effluents: challenges and opportunities for South Africa-a review.

Pervasive plastic wastes, pollution and detrimental environmental ethics are a serious threat in South Africa. Compared with global trends, most studies undertaken on plastic pollutions in water bodies across South Africa have generally been limited to marine and coastal waters. A literature review, for the last 40 years, demonstrated the scanty studies on the economic, social, health and cost implications of plastic entrainment into fresh water (sources of drinking water) and wastewater systems in South Africa. Hence, demonstrating a knowledge gap on this imperative issue, the inadequate and limited frameworks needed in assessing, evaluating and re-evaluating the menace of plastic pollution and entrainments into consumable water and wastewater treatment plants. This has hampered the local capacity, manpower, knowledge and understanding direly needed for mitigating these challenges. This work is necessitated because of the dire need in bridging the knowledge gap locally by adaptively reviewing possible challenges and opportunities for South Africa in meeting up the mandate of addressing this global threat. The emerging agreement amongst global policy-makers, educators and scientists is that environmental challenges, such as this, require, now more than ever, renewed ways of effective knowledge production and decision-making in tackling, holistically the menace of mismanaged plastic wastes and pollutions. These include but not limited to plastic education curriculum, synergised policies in fostering a circular plastic economy, overriding political will, innovative waste management systems, inclusive independent monitoring of plastic wastes, robust laws and effective enforcement strategies that are needed to promote better environmental ethics, mitigation and a sustainable environment.

Effects of stearic acid and irradiation alone and in combination on properties of amylose-lipid nanomaterial from high amylose maize starch.

This study determines the effects of stearic acid and gamma irradiation, alone and in combination, on properties of amylose-lipid nanomaterials from pasted high amylose maize starch (HAMS) with and without alpha amylase hydrolysis. HAMS was incorporated with stearic acid (0, 1.5% and 5%, w/w), irradiated at 0, 30 and 60 kGy and pasted under pressure in a rheometer. Isolated materials after thermostable alpha amylase or hot water washing were freeze-dried and characterised using differential scanning calorimetry (DSC), X-ray diffraction (XRD), Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM). The isolated materials contain amylose-lipid complexes (ALCs) as determined by DSC and XRD. Pasting of gamma irradiated HAMS produced type I ALCs, whereas that for un-irradiated HAMS produced type II ALCs. The ALCs occurred at nanoscale with sizes ranging from 10 to 110 nm as observed with AFM and TEM. Tailor-made ALCs nanomaterials can be produced from HAMS (with and without added stearic acid).

Fabrication of Bimetal CuFe2O4 Oxide Redox-Active Nanocatalyst for Oxidation of Pinene to Renewable Aroma Oxygenates.

This study report on the synthesis of spinel CuFe2O4 nanostructures by surfactant-assisted method. The catalysts were characterized by X-ray diffraction (XRD), laser Raman, transition electron microscope (TEM), scanning electron microscope (SEM), energy dispersive X-ray (EDX), hydrogen temperature programmed reduction (H2-TPR), and Brunauer-Teller-Emmett-Teller (BET) surface area techniques. CuFe2O4 was active for pinene oxidation using tertiary butyl hydroperoxide (TBHP) to pinene oxide, verbenol, and verbenone aroma oxygenates. Under optimized reaction conditions, the spinel CuFe2O4 catalyst could afford 80% pinene conversion at a combined verbenol/verbenone selectivity of 76% within the reaction time of 20 h. The changes in catalyst synthesis solvent composition ratios induced significantly varying redox, phases, and textural structure features, which resulted in various catalytic enhancement effect. Characterization results showed the spinel CuFe2O4 catalyst possessing less than 5 wt% impurity phases, Cu(OH)2, and CuO to afford the best catalytic performance. The CuFe2O4 catalyst was recyclable to up to five reaction cycles without loss of its activity. The recyclability of the bimetal CuFe2O4 oxide catalyst was simply rendered by use of an external magnet to separate it from the liquid solution.

Isolation and characterisation of nanoparticles from tef and maize starch modified with stearic acid.

Nanoparticles were isolated from tef and maize starch modified with added stearic acid after pasting at 90°C for 130min. This was followed by thermo-stable alpha-amylase hydrolysis of the paste. The resultant residues were then characterized using X-ray diffraction (XRD), differential scanning calorimetry (DSC), dynamic laser scattering particle size distribution (DLPSD), atomic force microscopy (AFM) and high-resolution transmission electron microscopy (HRTEM). XRD and DSC showed that the isolated residues consisted of amylose-lipid complexes. These complexes were type II with melting temperature above 104°C. DLPSD, AFM and HRTEM showed that the isolated tef and maize starch residues consisted of nanoparticles which became more distinct with increased hydrolysis time. The isolated tef and maize nanoparticles had distinct particles of about 3-10nm and 2.4-6.7nm, respectively and the yield was about 24-30%. The results demonstrated that distinct (physically separate) nanoparticles of less than 10nm can be isolated after formation during pasting of tef and maize starch with stearic acid. The production and isolation of the nanoparticles uses green chemistry principles and these nanoparticles can be used in food and non-food systems as nanofillers.

Microwave-assisted synthesis, characterization and antibacterial activity of Ag/ZnO nanoparticles supported bentonite clay.

Composites of silver-zinc oxide nanoparticles supported on bentonite clay were synthesized by the microwave-assisted synthesis method for use as an antibacterial material. Silver nitrate was used as the precursor of silver nanoparticles while zinc oxide nanoparticles were commercially sourced. The composites were characterized by powder X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier transform infrared (FTIR) and BET surface area measurements. XRD spectra showed peaks of silver confirming the formation of the silver and not of the silver nitrate or any other impurity of the metal. Meanwhile TEM confirmed the formation of silver and zinc oxide nanoparticles on the clay layers, with particle sizes ranging from 9-30 nm and 15-70 nm, respectively. The antibacterial activities of the composites were evaluated against Gram negative Escherichia coli bacteria and Gram positive Enterococcus faecalis bacteria by the disc diffusion method. Whereas both composites of Ag-clay and ZnO-clay showed good antibacterial activity against bacteria, a better antibacterial activity was observed with Ag/ZnO-clay composite. The results therefore reveal that Ag/ZnO-clay composite is a promising bactericide that can be used for deactivating microbes in water.