Effect of system of lamb rearing and season on early post-partum fertility of ewes and growth performance of lambs in Katahdin sheep.

The effect of season (S), lamb rearing system (RT) and grain supplementation (GS) on post-partum fertility in Katahdin ewes and growth in Katahdin lambs was evaluated. Katahdin ewes were bred to lamb in fall (n = 36) or spring (n = 56) and at approximately 2.5 months post-partum were randomly assigned to be permanently separated or to continue to suckle their lambs for an additional 3 months. All ewes were joined with rams following treatment to synchronize oestrus. Weaned (W, n = 84) and continuously suckled lambs (CSK, n = 88) were fed forage only (n = 84; hay and pasture for fall- and spring-born lambs respectively) or were supplemented (n = 88; 18% crude protein ration ad libitum) and all weighed biweekly. Ewes rebred in the fall had a shorter ram introduction to lambing interval (p < 0.05), higher prolificacy (p = 0.001) and higher lambing rates (p = 0.02); however, the proportion of ewes lambing was not affected by season. The first service lambing rate was lower in ewes continuously suckling lambs in the spring, but not in the fall breeding season (S × RT, p = 0.03). Lambs that continuously suckled their dams and were supplemented grew quicker and gained more (p < 0.001) than their weaned and unsupplemented counterparts. The effect of CSK on growth rate of lambs was greater in the spring (RT × S; p = 0.05), and the effect of supplementation on growth rate of lambs was greater in the fall (GS × S; p < 0.0001). In conclusion, Katahdin lambs achieved higher weight gains from continuous suckling and grain supplementation. Moreover, Katahdin ewes are capable of early rebreeding post-partum while suckling their lambs, which makes them suited for use in accelerated lambing programmes.

The relationship between circulating concentration of AMH and LH content in the follicle stimulating hormone (FSH) preparations on follicular growth and ovulatory response to superovulation in water buffaloes.

The relationship between circulating concentration of anti-mullerian hormone (AMH) and the LH content of follicle stimulating hormone (FSH) preparation on follicular growth and ovulatory response in water buffaloes was evaluated. A single blood sample was taken from cows (N=31; age: 9.06±0.98years) to determine systemic AMH. Animals with concentrations higher or lower than 194±30pg/ml were placed into LOW and HIGH AMH groups and were assigned randomly to be superovulated FSH containing either a high (FSHp, HLH) or low (FolltropinV, LLH) LH content. Follicular growth and ovulation were monitored using transrectal ultrasonography. In animals with HIGH systemic AMH, treatment with FSH with a high LH content was associated with more small follicles (AMH X FSH; P=0.02). AMH had no effect on small follicles in animals treated with LLH. Females with a HIGH AMH had greater numbers of small follicles (P=0.01) and total follicles (P=0.005) than LOW AMH cows. Animals treated with HLH had more small follicles (P=0.001) but fewer large (P<0.001) and total follicles (P=0.0005) than those treated with LLH. Among animals with HIGH AMH, those treated with LLH, ovulated more follicles than those treated with HLH. (AMH X FSH; P=0.03). In conclusion, selecting animals with high AMH concentration and the use of FSH preparations with a lower LH content may improve the superovulatory response in water buffaloes.

Use of jigs to standardise orthodontic bond testing.

OBJECTIVES: The aim of this study was to test whether the use of jigs for in vitro orthodontic bond strength testing helped to standardise the technique. METHODS: Two jigs were developed, one for mounting teeth with bonded brackets all in the same plane, and the second for debonding the brackets at 90 degrees to this plane. These jigs ensured the debonding force was applied in a controlled direction. Teeth mounted and debonded in this manner were compared with teeth tested with an existing protocol where the direction of the debonding force is not standardised. RESULTS: Although the same material was tested in both groups the use of jigs produced much higher mean bond strengths, and a reduced spread in the distribution of the results. CONCLUSIONS: To allow more legitimate comparisons between products and approaches, orthodontic bond strength testing must be universally standardised. The use of jigs to accurately direct the plane of the debonding force reduces the wide variations in results often seen in laboratory bond testing, improving standardisation.

Quantifying the kinematics of natural and prosthetic knee flexion.

A new method has been developed for quantifying knee kinematics during flexion. This method was used to measure knee kinematics from lateral radiographs taken at different angles of flexion with the two femoral condyles superimposed in each image, thus standardizing the plane of flexion-extension. When applied to the radiogaphs of five healthy male volunteers (age range 21-26 years), it showed that flexion was accompanied by translation between the articular surfaces. Knee kinematics were also measured in five patients after total knee replacement (TKR) surgery with a Kinemax Modular Total Knee prosthesis (Howmedica, Warsaw, Indiana). In the TKR patients, a translation was detected in three out of the five patients. This indicates that the prosthesis is capable of restoring normal kinematics, but does not always do so.

An automated method for assessing routine radiographs of patients with total hip replacements.

This paper describes a new, fully automated method of locating objects on radiographs of patients with total joint replacements (TJRs). A statistical computer model, known as an active shape model, was trained to identify the position of the femur, pelvis, stem and cup marker wire on radiographs of patients with Charnley total hip prostheses. Once trained, the model was able to locate these objects through a process of automatic image searching, despite their appearance depending on the orientation and anatomy of the patient. Experiments were carried out to test the accuracy with which the model was able to fit to previously unseen data and with which reference points could be calculated from the model points. The model was able to locate the femur and stem with a mean error of approximately 0.8 mm and a 95 per cent confidence limit of 1.7 mm. Once the model had successfully located these objects, the midpoint of the stem head could be calculated with a mean error of approximately 0.2 mm. Although the model has been trained on Charnley total hip replacements, the method is generic and so can be applied to radiographs of patients with any TJR. This paper shows that computer models can form the basis of a quick, automatic method of taking measurements from standard clinical radiographs.

Gonadotropin stimulation using P.G. 600® on reproductive success of non-lactating anestrous ewes.

The effect of stimulation with a gonadotropin preparation with combined follicle stimulating and luteininzing hormone like activity on reproductive success in anestrous ewes was evaluated. In Experiment 1, ewes of mixed breeding were treated with CIDR inserts (0.3g progesterone) for 5 days and were assigned randomly to receive either gonadotropin stimulation (3mL i.m. injection of P.G. 600®, 240IU eCG and 120IU hCG) at CIDR removal or no further treatment. Intact raddled rams were joined at insert removal for 30-35 days, and ewes were observed for indications of estrus after 4 days of ram exposure. Pregnancy diagnosis was conducted via transrectal ultrasonography at the time of ram removal and again 20-25 days. The second experiment was similar to Experiment 1, except treated ewes received the gonadotropin 1 day prior to insert removal. In Experiment 1, incidence of estrus was greater for treated ewes (P=0.01), and prolificacy tended to be greater in treated ewes (P=0.06). In Experiment 2, treated ewes had greater conception rates (P=0.01), pregnancy rates to first service (P=0.0007), and tended to have greater overall pregnancy rates than control ewes (P=0.07). A greater percentage of ewes lambed in the gonadotropin treated ewes than in ewes in the control group (P<0.0001), and overall lambing rates in treated ewes were greater than non-treated controls (P<0.0001). In conclusion, gonadotropin treatment 1 day prior to CIDR removal increased reproductive success in progesterone-treated anestrous ewes.