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1.
J Appl Microbiol ; 131(1): 135-145, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33251637

RESUMO

AIMS: The study was aimed to evaluate the antibacterial activity and efficacy of chestnut and quebracho wood extracts against Salmonella by in vitro assays and in vivo trials. METHODS AND RESULTS: The extracts showed inhibitory activity against Salmonella determined by the minimum inhibitory concentration method as well as on the adhesion and invasion of S. Gallinarum (SG) and S. Enteritidis (SE) in Caco-2 cells. Also, transmission electron microscopy revealed that extract-treated Salmonella showed disruption of cell walls and membranes, damage of the cytoplasm and tannin-protein aggregations. In addition, efficacy of the extracts to control SG and SE was evaluated in experimental infection trials in laying hens and broilers respectively. SE excretion was significantly reduced on days 5 (P < 0·01) and 12 (P < 0·025) only in the quebracho group. In the fowl typhoid infection model, hens that received the chestnut extract showed a significantly reduced mortality (P < 0·05). CONCLUSIONS: Our results evidence that these alternative natural products may be a useful tool to control Salmonella in poultry. SIGNIFICANCE AND IMPACT OF THE STUDY: Salmonella is a zoonotic pathogen usually associated with poultry production. This study provides information about the mechanism of antibacterial effects of chestnut and quebracho wood extracts to control Salmonella in poultry.


Assuntos
Galinhas/microbiologia , Extratos Vegetais/farmacologia , Salmonelose Animal/prevenção & controle , Salmonella/efeitos dos fármacos , Taninos/farmacologia , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Membrana Externa Bacteriana/efeitos dos fármacos , Células CACO-2 , Parede Celular/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Doenças das Aves Domésticas/microbiologia , Madeira/química
2.
Epilepsy Behav ; 102: 106655, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31812902

RESUMO

OBJECTIVE: Perampanel (PER) has been shown to be effective as an adjunctive therapy for controlling refractory focal-onset seizures (FOS). However, the information as early add-on for the treatment of FOS in the clinical practice is still scarce and must be further assessed. METHODS: An observational prospective study was conducted to evaluate the effectiveness of early add-on PER, assessed as 50% responders (seizure frequency reduced by at least 50% during the last 3 months as compared with baseline) rate at 6 and 12 months, in patients with FOS in the routine clinical practice of Spain. RESULTS: One hundred and thirteen patients (mean age: 40.3 years, 51.3% male) with FOS received PER as early add-on (1st add-on: 37.2% and 2nd: 62.8%) for a mean exposure of 11 months (mean PER dose: 6.3 mg/day at month 12). At 6 months, 50.4% and 20.4% of the patients were responders and seizure-free (respectively) relative to baseline (3 months prior to PER initiation), and at 12 months, 68.1% and 26.5% of the patients were responders and seizure-free (respectively), relative to baseline (3 months prior to PER initiation). The retention rate at 6 and 12 months was 83.2% and 80.5%, respectively. The percentage of seizure-free patients at 12 months was significantly (p = 0.033) higher when PER was added as first vs. second add-on. The number of concomitant antiepileptic drugs (AEDs) was significantly reduced from baseline to 6 and 12 months (p = 0.001). Treatment was simplified in 23.9% of patients at the end of the observation period. Drug-related adverse events (AEs), most mild or moderate, were reported in 30.1% of patients, with irritability (8%) and dizziness (7.1%) as the most frequent ones. CONCLUSIONS: This is the first observational, prospective study to evaluate efficacy and safety of early adjunctive treatment with PER in patients with focal epilepsy at 12 months. Perampanel demonstrated a good efficacy and safety profile when used at a median dose of 6 mg/day, regardless of the combination with other AEDs. Adverse events were mild or moderate, with dizziness being the most frequent one.


Assuntos
Anticonvulsivantes/administração & dosagem , Epilepsia/tratamento farmacológico , Epilepsia/epidemiologia , Piridonas/administração & dosagem , Convulsões/tratamento farmacológico , Convulsões/epidemiologia , Adulto , Anticonvulsivantes/efeitos adversos , Tontura/induzido quimicamente , Quimioterapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nitrilas , Estudos Prospectivos , Piridonas/efeitos adversos , Espanha/epidemiologia , Resultado do Tratamento , Adulto Jovem
3.
HIV Med ; 16(5): 273-9, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25523089

RESUMO

OBJECTIVES: The aim of the study was to determine the prevalence of potential clinically significant drug interactions (CSDIs) in HIV-positive individuals and to identify associated risk factors. METHODS: A cross-sectional study was conducted including all HIV-infected out-patients attending the Pharmacy Service of a regional reference hospital in Murcia, south-eastern Spain. The complete treatment was screened for possible CSDIs using the Spanish College of Pharmacists' online software resource, bot. Additionally, the severity level of the CSDIs involving antiretroviral (ARV) drugs was compared with that established in the specific antiretroviral database InteraccionesHIV.com. Multivariate logistic regression was used to identify associated risk factors. RESULTS: Two hundred and sixty-eight patients were included in the study. A total of 292 potential drug interactions were identified, of which 102 (34.9%) were CSDIs, of which 52.9% involved ARV drugs. Seven therapeutic drug classes were involved in 75% of CSDIs (protease inhibitors, benzodiazepines, nonsteroidal anti-inflammatory drugs, nonnucleoside reverse transcriptase inhibitors, corticosteroids, antithrombotics and proton pump inhibitors). Factors independently associated with CSDIs were treatment with more than five drugs [odds ratio (OR) 15.1; 95% confidence interval (CI) 6.3-36.2], and treatment with a protease inhibitor (OR 5.3; 95% CI 2.4-11.74). CONCLUSIONS: The findings of this study suggest that the prevalence of clinically relevant drug-drug interactions is high in HIV-infected patients, and could represent a major health problem. Awareness, recognition and management of drug interactions are important in optimizing the pharmaceutical care of HIV-infected patients and helping to prevent adverse events and/or loss of efficacy of the drugs administered.


Assuntos
Fármacos Anti-HIV/administração & dosagem , Interações Medicamentosas , Infecções por HIV/tratamento farmacológico , Hepatite B/tratamento farmacológico , Hepatite C/tratamento farmacológico , Adulto , Sistemas de Notificação de Reações Adversas a Medicamentos , Idoso , Idoso de 80 Anos ou mais , Fármacos Anti-HIV/efeitos adversos , Comorbidade , Estudos Transversais , Esquema de Medicação , Quimioterapia Combinada , Feminino , Inibidores da Fusão de HIV/administração & dosagem , Inibidores da Fusão de HIV/efeitos adversos , Inibidores de Integrase de HIV/administração & dosagem , Inibidores de Integrase de HIV/efeitos adversos , Inibidores da Protease de HIV/administração & dosagem , Inibidores da Protease de HIV/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Inibidores da Fosfodiesterase 5/administração & dosagem , Inibidores da Fosfodiesterase 5/efeitos adversos , Padrões de Prática Médica , Prevalência , Inibidores da Transcriptase Reversa/administração & dosagem , Inibidores da Transcriptase Reversa/efeitos adversos , Fatores de Risco , Espanha/epidemiologia
5.
Anaerobe ; 34: 139-45, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26037239

RESUMO

Tannins added in the diet are being used to improve nutrition and health in farm animals as an alternative to antibiotic growth promoters and to control enteric clostridial diseases. However, the capacity of Clostridium perfringens to develop resistance under the selective pressure of tannins is unknown. The purpose of this study was to determine if C. perfringens possess the ability to develop resistance against tannins in comparison with antimicrobial agents. Susceptibility for 7 AGPs (antimicrobial growth promoters), 9 therapeutic antimicrobials and 2 tannin based extracts was determined for 30 C. perfringens strains isolated from poultry and cattle. Two susceptible strains were selected and cultured in presence of sub-inhibitory concentrations of tannins and AGPs for resistant sub-populations selection. Tannin resistance of C. perfringens isolates from both animal species revealed no statistically significant differences in MICs (minimum inhibitory concentration). Poultry isolates showed higher MICs to several AGPs compared with cattle isolates. All isolates were susceptible to the therapeutic antimicrobials tested, but avian isolates showed a significantly lower susceptibility to these antimicrobials which was highly correlated with an increased resistance to bacitracin and others AGPs. In-vitro selection of resistant clones suggests that C. perfringens was unable to develop resistance against tannins at least compared to AGPs like bacitracin and avilamycin. Avian origin strains, which were previously exposed to antibiotics showed higher resistance, compared to cattle origin strains. These results suggest that the evolution of resistance against tannins in C. perfringens would be more difficult and slower than to the determined AGPs.


Assuntos
Anti-Infecciosos/metabolismo , Clostridium perfringens/efeitos dos fármacos , Tolerância a Medicamentos , Proantocianidinas/metabolismo , Animais , Bovinos , Clostridium perfringens/isolamento & purificação , Hidrólise , Testes de Sensibilidade Microbiana , Mutação , Taxa de Mutação , Aves Domésticas , Seleção Genética , Inoculações Seriadas
6.
Anaerobe ; 20: 1-4, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23354004

RESUMO

Clostridium perfringens type E is considered a rare toxinotype and an infrequent cause of enterotoxemia of lambs, calves, and rabbits. Until now, only cases of young animal of C. perfringens type E bovine enterotoxemia, characterized by hemorrhagic enteritis and sudden death, have been reported. The present report details the genotypic characterization of C. perfringens type E isolates obtained from intestinal samples of adult cattle during an outbreak of enterotoxemia in Argentina. The sequences of several housekeeping genes of these isolates were analyzed and compared with those obtained from calves in North America showing a clonal unique lineage.


Assuntos
Doenças dos Bovinos/epidemiologia , Infecções por Clostridium/veterinária , Clostridium perfringens/genética , Clostridium perfringens/isolamento & purificação , Morte Súbita/veterinária , Surtos de Doenças/veterinária , Enterotoxemia/epidemiologia , Animais , Argentina/epidemiologia , Proteínas de Bactérias/genética , Bovinos , Doenças dos Bovinos/microbiologia , Infecções por Clostridium/epidemiologia , Infecções por Clostridium/microbiologia , Clostridium perfringens/classificação , Clostridium perfringens/patogenicidade , Morte Súbita/epidemiologia , Morte Súbita/etiologia , Enterotoxemia/microbiologia , Genótipo , Dados de Sequência Molecular , Tipagem de Sequências Multilocus/métodos , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Síndrome , Fatores de Virulência/genética
7.
J Exp Zool A Ecol Integr Physiol ; 339(9): 816-824, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-37434416

RESUMO

Different blood parasites can co-infect natural populations of lizards. However, our knowledge of the host's ability to recover from them (i.e., significantly reduce parasitemia levels) is scarce. This has interest from an ecological immunology perspective. Herein, we investigate the host recovery ability in males of the lizard Psammodromus algirus infected by parasite genera Schellackia and Karyolysus. The role of lizard hosts is dissimilar in the life cycle of these two parasites, and thus different immune control of the infections is expected by the vertebrate host. As Schellackia performs both sexual and asexual reproduction cycles in lizards, we expect a better immune control by its vertebrate hosts. On the contrary, Karyolysus performs sexual reproductive cycles in vectors, hence we expect lower immune control by the lizards. We carried out a reciprocal translocation experiment during the lizards' mating season to evaluate both parasitemia and leukocyte profiles in male lizards, being one of the sampling plots close to a road with moderate traffic. These circumstances provide a combination of extrinsic (environmental stress) and intrinsic factors (reproductive vs. immune trade-offs) that may influence host's recovery ability. We recaptured 33% of the lizards, with a similar proportion in control and translocated groups. Karyolysus infected 92.3% and Schellackia 38.5% of these lizards. Hosts demonstrated ability to significantly reduce parasitemia of Schellackia but not of Karyolysus. This suggests, in line with our predictions, a differential immune relationship of lizards with these parasites, at time that supports that parasites with different phylogenetic origins should be analyzed separately in investigations of their effects on hosts. Furthermore, lizards close to the road underwent a stronger upregulation of lymphocytes and monocytes when translocated far from the road, suggesting a putative greater exposure to pathogens in the latter area.


Assuntos
Lagartos , Parasitos , Masculino , Animais , Filogenia , Parasitemia/veterinária , Parasitemia/parasitologia , Lagartos/parasitologia , Análise de Sequência de DNA
9.
Rev Clin Esp (Barc) ; 218(3): 121-127, 2018 Apr.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-29329765

RESUMO

INTRODUCTION AND OBJECTIVES: The trabecular bone score (TBS) is an imaging technique that assesses the condition of the trabecular microarchitecture. Preliminary results suggest that TBS, along with the bone mineral density assessment, could improve the calculation of the osteoporotic fracture risk. The aim of this study was to analyse TBS values and their relationship with the clinical characteristics, bone mineral density and history of fractures of a cohort of posmenopausal women. MATERIAL AND METHODS: We analysed 2,257 posmenopausal women from the FRODOS cohort, which was created to determine the risk factors for osteoporotic fracture through a clinical survey and bone densitometry with vertebral morphometry. TBS was applied to the densitometry images. TBS values ≤1230 were considered indicative of degraded microarchitecture. We performed a simple and multiple linear regression to determine the factors associated with this index. RESULTS: The mean TBS value in L1-L4 was 1.203±0.121. Some 55.3% of the women showed values indicating degraded microarchitecture. In the multiple linear regression analysis, the factors associated with low TBS values were age, weight, height, spinal T-score, glucocorticoid treatment, presence of type 2 diabetes and a history of fractures due to frailty. CONCLUSIONS: TBS showed microarchitecture degradation values in the participants of the FRODOS cohort and was associated with anthropometric factors, low bone mineral density values, the presence of fractures, a history of type 2 diabetes mellitus and the use of glucocorticoids.

10.
Rev Sci Instrum ; 78(3): 034702, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17411205

RESUMO

This article discusses the operation of a modular generator topology, which has been developed for high-frequency (kHz), high-voltage (kV) pulsed applications. The proposed generator uses individual modules, each one consisting of a pulse circuit based on a modified forward converter, which takes advantage of the required low duty cycle to operate with a low voltage clamp reset circuit for the step-up transformer. This reduces the maximum voltage on the semiconductor devices of both primary and secondary transformer sides. The secondary winding of each step-up transformer is series connected, delivering a fraction of the total voltage. Each individual pulsed module is supplied via an isolation transformer. The assembled modular laboratorial prototype, with three 5 kV modules, 800 V semiconductor switches, and 1:10 step-up transformers, has 80% efficiency, and is capable of delivering, into resistive loads, -15 kV1 A pulses with 5 micros width, 10 kHz repetition rate, with less than 1 micros pulse rise time. Experimental results for resistive loads are presented and discussed.

11.
Int J Clin Pharmacol Ther ; 44(4): 154-62, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16625984

RESUMO

OBJECTIVE: To assess the effect of a 2-week treatment with dexibuprofen, in comparison with ibuprofen and diclofenac, on pepsinogen plasma concentrations and gastrointestinal mucosa, as well as the correlation of these changes with gastrointestinal mucosal injury. METHODS: 60 patients with rheumatologic disease in chronic therapy with NSAID, were included. After a 7-day run-in period patients were randomly assigned to receive a 14-day treatment with dexibuprofen (Group A; Day 1 - 3 = 400 mg t.i.d; Day 4 - 14 = 400 mg b.i.d.), ibuprofen (Group B; Day 1 - 3 = 800 mg t.i.d; Day 4 -14 = 800 mg b.i.d.) or diclofenac (Group C; Day 1 - 3 = 50 mg t.i.d; Day 4 - 14 = 50 mg b.i.d.). Upper gastrointestinal endoscopy (Day 15), capsule-endoscopy (Day 16, 7 patients of each group) and determination of pepsinogen plasma concentrations were performed (basal and Day 15). A semiquantitative scale was designed for the assessment of the gastrointestinal mucosa. RESULTS: No differences in plasma pepsinogen were found between treatment groups or gastrointestinal injury grades or between basal and post-therapy determinations. Dexibuprofen showed gastroduodenal mucosal injury in fewer patients (42.1%) than was the case with ibuprofen (5%; p = 0.003) and diclofenac (30%; p = N.S.). Dexibuprofen administration was also associated with more patients having no intestinal mucosal damage (42.86% vs. 28.7% in the diclofenac group and 14.29% in the ibuprofen group; p = 0.0175). The rate of clinical adverse events was similar in Groups A, B and C (28%, 38% and 34%). CONCLUSIONS: Dexibuprofen showed a lower rate of gastroduodenal and intestinal mucosal injury. This effect was not mediated by modifications of plasma pepsinogen levels.


Assuntos
Inibidores de Ciclo-Oxigenase/efeitos adversos , Diclofenaco/efeitos adversos , Ibuprofeno/efeitos adversos , Indoprofen/efeitos adversos , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Pepsinogênio A/sangue , Administração Oral , Adulto , Inibidores de Ciclo-Oxigenase/farmacologia , Diclofenaco/farmacologia , Endoscopia Gastrointestinal , Feminino , Humanos , Ibuprofeno/farmacologia , Indoprofen/farmacologia , Masculino , Pessoa de Meia-Idade , Pepsinogênio A/efeitos dos fármacos , Doenças Reumáticas/tratamento farmacológico , Doenças Reumáticas/patologia
12.
Biomed Res Int ; 2016: 3278359, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27747227

RESUMO

Necrotic enteritis (NE) is an important concern in poultry industry since it causes economic losses, increased mortality, reduction of bird welfare, and contamination of chicken products for human consumption. For decades, the use of in-feed antimicrobial growth promoters (AGPs) has been the main strategy to control intestinal pathogens including Clostridium perfringens (CP), the causative agent of NE. However, the use of AGPs in animal diet has been linked to the emergence and transmission of antimicrobial resistance through food-borne microorganisms, which has led to the ban of AGPs in many countries. This scenario has challenged the poultry industry to search for safer alternative products in order to prevent NE. In this context, the utilization of natural plant extracts with antimicrobial properties appears as a promising and feasible tool to control NE in chicken. In this paper, we review the scientific studies analyzing the potential of plant extracts as alternative feed additives to reduce NE in poultry, with focus on two types of plant products that arise as promising candidates: tannins and essential oils. Some of these products showed antimicrobial activity against CP and coccidia in vitro and in vivo and are able to increase productive performance, emulating the bioactive properties of AGPs.


Assuntos
Anti-Infecciosos/uso terapêutico , Infecções por Clostridium/veterinária , Enterite/tratamento farmacológico , Necrose/tratamento farmacológico , Óleos Voláteis/química , Extratos Vegetais/química , Doenças das Aves Domésticas/tratamento farmacológico , Taninos/química , Animais , Antibacterianos/uso terapêutico , Anti-Infecciosos/química , Infecções por Clostridium/tratamento farmacológico , Clostridium perfringens/efeitos dos fármacos , Farmacorresistência Bacteriana , Tolerância a Medicamentos , Microbiologia de Alimentos , Testes de Sensibilidade Microbiana , Aves Domésticas
15.
Bioelectrochemistry ; 103: 60-4, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25220563

RESUMO

This work describes the utilization of Pulsed Electric Fields to control the protozoan contamination of a microalgae culture, in an industrial 2.7 m(3) microalgae photobioreactor. The contaminated culture was treated with Pulsed Electric Fields, PEF, for 6h with an average of 900 V/cm, 65 µs pulses of 50 Hz. Working with recirculation, all the culture was uniformly exposed to the PEF throughout the assay. The development of the microalgae and protozoan populations was followed and the results showed that PEF is effective on the selective elimination of protozoa from microalgae cultures, inflicting on the protozoa growth halt, death or cell rupture, without affecting microalgae productivity. Specifically, the results show a reduction of the active protozoan population of 87% after 6h treatment and 100% after few days of normal cultivation regime. At the same time, microalgae growth rate remained unaffected.


Assuntos
Biotecnologia/métodos , Técnicas Eletroquímicas/métodos , Microalgas/crescimento & desenvolvimento , Microalgas/parasitologia , Biotecnologia/instrumentação , Membrana Celular , Desenho de Equipamento , Fotobiorreatores
16.
Br J Pharmacol ; 172(12): 3159-76, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25712370

RESUMO

BACKGROUND AND PURPOSE: Toll-like receptor 4 (TLR4) signalling contributes to inflammatory cardiovascular diseases, but its role in hypertension and the associated vascular damage is not known. We investigated whether TLR4 activation contributed to angiotensin II (AngII)-induced hypertension and the associated vascular structural, mechanical and functional alterations. EXPERIMENTAL APPROACH: AngII was infused (1.44 mg · kg(-1) · day(-1), s.c.) for 2 weeks in C57BL6 mice, treated with a neutralizing anti-TLR4 antibody or IgG (1 µg · day(-1); systolic BP (SBP) and aortic cytokine levels were measured. Structural, mechanical and contractile properties of aortic and mesenteric arterial segments were measured with myography and histology. RT-PCR and Western blotting were used to analyse these tissues and cultured vascular smooth muscle cells (VSMC) from hypertensive rats (SHR). KEY RESULTS: Aortic TLR4 mRNA levels were raised by AngII infusion. Anti-TLR4 antibody treatment of AngII-treated mice normalised: (i) increased SBP and TNF-α, IL-6 and CCL2 levels; (ii) vascular structural and mechanical changes; (iii) altered aortic phenylephrine- and ACh-induced responses; (iv) increased NOX-1 mRNA levels, superoxide anion production and NAD(P)H oxidase activity and effects of catalase, apocynin, ML-171 and Mito-TEMPO on vascular responses; and (v) reduced NO release and effects of L-NAME on phenylephrine-induced contraction. In VSMC, the MyD88 inhibitor ST-2825 reduced AngII-induced NAD(P)H oxidase activity. The TLR4 inhibitor CLI-095 reduced AngII-induced increased phospho-JNK1/2 and p65 NF-κB subunit nuclear protein expression. CONCLUSIONS AND IMPLICATIONS: TLR4 up-regulation by AngII contributed to the inflammation, endothelial dysfunction, vascular remodelling and stiffness associated with hypertension by mechanisms involving oxidative stress. MyD88-dependent activation and JNK/NF-κB signalling pathways participated in these alterations.


Assuntos
Angiotensina II/administração & dosagem , Hipertensão/fisiopatologia , Receptor 4 Toll-Like/metabolismo , Remodelação Vascular/fisiologia , Animais , Aorta/metabolismo , Pressão Sanguínea , Endotélio Vascular/patologia , Hipertensão/genética , Inflamação/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo/fisiologia , Ratos , Ratos Endogâmicos SHR , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 4 Toll-Like/genética , Regulação para Cima
17.
Clin Nutr ; 22(5): 463-71, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14512034

RESUMO

BACKGROUND & AIMS: Dietary fiber has been proven to be beneficial in maintaining remission in human ulcerative colitis, an effect related with an increased luminal production of short-chain fatty acids (SCFA). The aim of the present study was to further investigate the mechanisms involved in the intestinal anti-inflammatory effects of dietary fiber in an experimental model of rat colitis. METHODS: HLA-B27 transgenic rats (8-10 weeks old) were fed a fiber-supplemented diet (5% Plantago ovata seeds) for 13 weeks before evaluation of the colonic inflammatory status, both histologically and biochemically. The luminal colonic production of SCFA was quantified. In vitro studies were also performed to test the interaction between two SCFA (butyrate and propionate) as inhibitors of cytokine production in THP-1 cells. RESULTS: Dietary fiber supplementation ameliorated the development of colonic inflammation in transgenic rats as evidenced by an improvement of intestinal cytoarchitecture. This effect was associated with a decrease in some of the pro-inflammatory mediators involved in the inflammatory process: nitric oxide, leukotriene B(4), tumor necrosis factor alpha (TNFalpha). The intestinal contents from fiber-treated colitic rats showed a significant higher production of SCFA, butyrate and propionate, than non-treated colitic animals. In vitro studies revealed a synergistic inhibitory effect of butyrate and propionate on TNFalpha production. CONCLUSIONS: Dietary fiber supplementation ameliorated colonic damage in HLA-B27 transgenic rats. This effects was associated with an increased production of SCFA, which can act synergistically in inhibiting the production of pro-inflammatory mediators.


Assuntos
Colite Ulcerativa/dietoterapia , Fibras na Dieta/uso terapêutico , Ácidos Graxos Voláteis/biossíntese , Mediadores da Inflamação , Animais , Células Cultivadas , Colite Ulcerativa/metabolismo , Colo/citologia , Colo/patologia , Modelos Animais de Doenças , Ácidos Graxos Voláteis/farmacologia , Feminino , Antígeno HLA-B27/genética , Organismos Geneticamente Modificados , Plantago , Psyllium , Distribuição Aleatória , Ratos , Ratos Endogâmicos F344 , Sementes , Resultado do Tratamento , Fator de Necrose Tumoral alfa/antagonistas & inibidores
18.
Int J Oral Maxillofac Surg ; 27(2): 92-3, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9565262

RESUMO

A case of isolated unilateral temporalis muscle hypertrophy is presented. A review of the literature revealed only one report of temporalis hypertrophy, probably caused by psychogenic muscle hyperactivity. The temporalis muscle hypertrophy in the patient described was idiopathic and its management was surgical.


Assuntos
Músculo Temporal/patologia , Adolescente , Feminino , Humanos , Hipertrofia/cirurgia , Músculo Temporal/cirurgia
19.
Int J Oral Maxillofac Surg ; 24(6): 445-8, 1995 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8636642

RESUMO

In an experimental model in the rat, a nonhealing bone defect was created in the left ascending mandibular ramus to test the effect of particulate porous hydroxyapatite (HA) on osteoinduction of demineralized bone autografts. The bone fragment removed was demineralized in HCl and used as an autograft for mandibular reconstruction. Granules of HA were added to the lingual and vestibular surfaces of the graft. The effect of this material was evaluated by determining the number of mesenchymal cells induced in the biomaterial and the central and peripheral zones of the bone graft, at 2-and 6-week intervals. The results show that the sites containing HA showed inhibition of osteoinduction by the bone matrix. In all groups, a proliferative gradient from the peripheral zone toward the center of the bone was observed. Similarly, the HA experienced a greater cellular increase in the regions in contact with the demineralized bone matrix.


Assuntos
Materiais Biocompatíveis/química , Substitutos Ósseos/química , Transplante Ósseo/fisiologia , Durapatita/química , Mandíbula/cirurgia , Osteogênese , Animais , Matriz Óssea/patologia , Matriz Óssea/fisiopatologia , Transplante Ósseo/patologia , Divisão Celular , Técnica de Descalcificação , Modelos Animais de Doenças , Mandíbula/patologia , Mandíbula/fisiopatologia , Doenças Mandibulares/cirurgia , Mesoderma/patologia , Osseointegração , Porosidade , Ratos , Ratos Wistar , Transplante Autólogo
20.
J Reprod Med ; 40(8): 579-84, 1995 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7473455

RESUMO

OBJECTIVE: To evaluate the histologic effects and permeability rate of different suture materials in microsurgical anastomosis of the left uterine horn in Wistar rats. STUDY DESIGN: In this histologic study, 81 female Wistar rats were used. A transverse section and anastomosis were performed using three surgical techniques. The rats were divided into 15 experimental groups based on evaluation of three variations in microsurgical technique: (1) one-layer transmucosal anastomosis, (2) one-layer extramucosal anastomosis, and (3) two-layer transmucosal anastomosis. With each technique, five suture materials were used: 7/0 polyglycolic acid, 6/0 polyglactin, 7/0 polydioxanone, 7/0 silk and 7/0 polypropylene. RESULTS: The permeability rate was 86%. Polydioxanone persisted in 41% of cases at 90 days. Abnormal proliferation of mucosal epithelium was verified in 10% of cases. Polypropylene was responsible for the majority of foreign-body granulomas. Polydioxanone produced the smallest inflammatory reaction. CONCLUSION: The type of suture material did not influence the permeability rate. Use of nonabsorbable suture material altered the histologic structure the most and produced the greatest tissue reaction. The best results were obtained with polydioxanone. Monofilial sutures gave rise to fewer structural alterations than did multifilament sutures.


Assuntos
Suturas , Útero/patologia , Absorção , Anastomose Cirúrgica/métodos , Animais , Epitélio/patologia , Feminino , Granuloma de Corpo Estranho/patologia , Inflamação/patologia , Microcirurgia , Mucosa/patologia , Neovascularização Patológica , Ratos , Ratos Wistar , Útero/irrigação sanguínea , Útero/cirurgia
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