RESUMO
Metalloenzymes often utilize radicals in order to facilitate chemical reactions. Recently, DeGrado and co-workers have discovered that model proteins can efficiently stabilize semiquinone radical anion produced by oxidation of 3,5-di-tert-butylcatechol (DTBC) in the presence of two zinc ions. Here, we show that the number and the nature of metal ions have relatively minor effect on semiquinone stabilization in model proteins, with a single metal ion being sufficient for radical stabilization. The radical is stabilized by both metal ion, hydrophobic sequestration, and interactions with the hydrophilic residues in the protein interior resulting in a remarkable, nearly 500â mV change in the redox potential of the SQ. - /catechol couple compared to bulk aqueous solution. Moreover, we have created 4G-UFsc, a single metal ion-binding protein with pm affinity for zinc that is higher than any other reported model systems and is on par with many natural zinc-containing proteins. We expect that the robust and easy-to-modify DFsc/UFsc family of proteins will become a versatile tool for mechanistic model studies of metalloenzymes.
Assuntos
Benzoquinonas/química , Metaloproteínas/química , Metais/química , Sequência de Aminoácidos , Sítios de Ligação , Catecóis/química , Interações Hidrofóbicas e Hidrofílicas , Íons/química , Cinética , Ligantes , Modelos Moleculares , Oxirredução , Ligação Proteica , Conformação Proteica , Estabilidade Proteica/efeitos dos fármacos , TermodinâmicaRESUMO
A computationally designed, allosterically regulated catalyst (CaM M144H) produced by substituting a single residue in calmodulin, a non-enzymatic protein, is capable of efficient and site selective post-translational acylation of lysines in peptides with highly diverse sequences. Calmodulin's binding partners are involved in regulating a large number of cellular processes; this new chemical-biology tool will help to identify them and provide structural insight into their interactions with calmodulin.
Assuntos
Substituição de Aminoácidos , Calmodulina/genética , Calmodulina/metabolismo , Lisina/metabolismo , Peptídeos/metabolismo , Processamento de Proteína Pós-Traducional , Acilação , Regulação Alostérica , Sequência de Aminoácidos , Animais , Sítios de Ligação , Calmodulina/química , Humanos , Lisina/análise , Modelos Moleculares , Peptídeos/química , Ligação Proteica , Engenharia de ProteínasRESUMO
Minimalist enzymes designed to catalyze model reactions provide useful starting points for creating catalysts for practically important chemical transformations. We have shown that Kemp eliminases of the AlleyCat family facilitate conversion of leflunomide (an immunosupressor pro-drug) to its active form teriflunomide with outstanding rate enhancement (nearly four orders of magnitude) and catalytic proficiency (more than seven orders of magnitude) without any additional optimization. This remarkable activity is achieved by properly positioning the substrate in close proximity to the catalytic glutamate with very high pKa.