RESUMO
Polychlorinated biphenyl (PCB) congeners differentially reduce serum thyroxine (T(4)) in rats, but little is known about their ability to affect biliary excretion of T(4). Thus, male Sprague-Dawley rats were orally administered Aroclor-1254, Aroclor-1242 (32 mg/kg per day), PCB-95, PCB-99, PCB-118 (16 mg/kg per day), PCB-126 (40 µg/kg per day), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (3.9 µg/kg per day), or corn oil for 7 days. Twenty-four hours after the last dose, [(125)I]T(4) was administered intravenously, and blood, bile, and urine samples were collected for quantifying [(125)I]T(4) and in bile [(125)I]T(4) metabolites. Serum T(4) concentrations were reduced by all treatments, but dramatic reductions occurred in response to Aroclor-1254, PCB-99 [phenobarbital (PB)-type congener], and PCB-118 (mixed-type congener). None of the treatments increased urinary excretion of [(125)I]T(4). Aroclor-1254, PCB-118, TCDD, and PCB-126 (TCDD-type congener) increased biliary excretion of T(4)-glucuronide by 850, 756, 710, and 573%, respectively, corresponding to marked induction of hepatic UDP-glucuronosyltransferase (UGT) activity toward T(4). PCB-95 and PCB-99 did not induce UGT activity; therefore, the increased biliary excretion of T(4)-glucuronide was related to the affinity of congeners for the aryl hydrocarbon receptor. The disappearance of [(125)I]T(4) from serum was rapid (within 15-min) and was increased by Aroclor-1254, PCB-99 and PCB-118. Thus, reductions in serum T(4) in response to PCBs did not always correspond with UGT activity toward T(4) or with increased biliary excretion of T(4)-glucuronide. The rapid disappearance of [(125)I]T(4) from the serum of rats treated with PB-like PCBs suggests that increased tissue uptake of T(4) is an additional mechanism by which PCBs may reduce serum T(4).
Assuntos
Bile/efeitos dos fármacos , Bile/metabolismo , Bifenilos Policlorados/farmacologia , Tiroxina/sangue , Tiroxina/metabolismo , Animais , Peso Corporal/efeitos dos fármacos , Glucuronídeos/metabolismo , Glucuronosiltransferase/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , Dibenzodioxinas Policloradas/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores de Hidrocarboneto Arílico/metabolismoRESUMO
The neurotoxic heavy metal trimethyltin (TMT) primarily damages neurons of the hippocampus and limbic areas of the temporal lobe, and causes a dose-dependent decrease in the polysialated form of the neural cell adhesion molecule (PSA-NCAM) in the mouse hippocampus. In the current study, we attempted to associate deficits in spatial learning following TMT exposure at various stages in learning with changes in levels of NCAM-180 and PSA-NCAM in both the hippocampus and frontal cortex. Mice were treated with TMT either before or after training on a spatial learning paradigm and examined for changes in NCAM and PSA-NCAM 12h later. In the first set of experiments, male BALB/c mice were injected with TMT (2.25 mg/kg) or saline i.p. and tested 24-168 h later using hidden and visible versions of the water maze, as well as light avoidance and motor activity. Mice in both treated and control groups which demonstrated a significant improvement in water maze performance also showed an elevation in hippocampal PSA-NCAM at all time points examined. TMT exposure impaired spatial learning and blocked learning-induced elevations in PSA-NCAM expression 24-96 h post-treatment, but these deficits disappeared by 168 h post-treatment. Mice exposed to TMT during reconsolidation of spatial learning (after repeated water maze training) demonstrated a mild and transient difference in escape latency compared to saline exposed mice. TMT administration during this period did not result in the attenuation of PSA-NCAM expression observed when animals were exposed before training. These results confirm a specific role for PSA-NCAM in acquisition and consolidation of spatial memory.
Assuntos
Comportamento Animal/efeitos dos fármacos , Molécula L1 de Adesão de Célula Nervosa/biossíntese , Ácidos Siálicos/biossíntese , Compostos de Trimetilestanho/toxicidade , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Western Blotting , Química Encefálica/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Atividade Motora/efeitos dos fármacosRESUMO
Curcumin (diferuloylmethane), a yellow pigment that is obtained from the rhizomes of Curcuma longa Linn., is a major component of turmeric and is commonly used as a spice and food-coloring agent. The inhibitory effects of feeding commercial grade curcumin (77% curcumin, 17% demethoxycurcumin, and 3% bisdemethoxycurcumin) in AIN 76A diet on carcinogen-induced tumorigenesis in the forestomach, duodenum, and colon of mice were evaluated. Administration p.o. of commercial grade curcumin in the diet inhibited benzo(a)pyrene-induced forestomach tumorigenesis in A/J mice, N-ethyl-N'-nitro-N-nitrosoguanidine-induced duodenal tumorigenesis in C57BL/6 mice, and azoxymethane (AOM)-induced colon tumorigenesis in CF-1 mice. Dietary commercial grade curcumin was given to mice at: (a) 2 weeks before, during, and for 1 week after carcinogen administration (during the initiation period); (b) 1 week after carcinogen treatment until the end of the experiment (during the postinitiation period); or (c) during both the initiation and postinitiation periods. Feeding 0.5-2.0% commercial grade curcumin in the diet decreased the number of benzo(a)pyrene-induced forestomach tumors per mouse by 51-53% when administered during the initiation period and 47-67% when administered during the postinitiation period. Feeding 0.5-2.0% commercial grade curcumin in the diet decreased the number of N-ethyl-N'-nitro-N-nitrosoguanidine-induced duodenal tumors per mouse by 47-77% when administered during the postinitiation period. Administration of 0.5-4.0% commercial grade curcumin in the diet both during the initiation and postinitation periods decreased the number of AOM-induced colon tumors per mouse by 51-62%. Administration of 2% commercial grade curcumin in the diet inhibited the number of AOM-induced colon tumors per mouse by 66% when fed during the initiation period and 25% when fed during the postinitiation period. The ability of commercial grade curcumin to inhibit AOM-induced colon tumorigenesis is comparable to that of pure curcumin (purity greater than 98%). Administration of pure or commercial grade curcumin in the diet to AOM-treated mice resulted in development of colon tumors which were generally smaller in number and size as compared to the control group of AOM-treated mice. These results indicate that not only did curcumin inhibit the number of tumors per mouse and the percentage of mice with tumors but it also reduced tumor size. Histopathological examination of the tumors showed that dietary curcumin inhibited the number of papillomas and squamous cell carcinomas of the forestomach as well as the number of adenomas and adenocarcinomas of the duodenum and colon.
Assuntos
Neoplasias do Colo/prevenção & controle , Curcumina/farmacologia , Neoplasias Duodenais/prevenção & controle , Neoplasias Gástricas/prevenção & controle , Adenocarcinoma/induzido quimicamente , Adenocarcinoma/prevenção & controle , Adenoma/induzido quimicamente , Adenoma/prevenção & controle , Adenoma Viloso/induzido quimicamente , Adenoma Viloso/prevenção & controle , Animais , Azoximetano , Benzo(a)pireno , Carcinógenos , Neoplasias do Colo/induzido quimicamente , Curcumina/administração & dosagem , Neoplasias Duodenais/induzido quimicamente , Feminino , Masculino , Metilnitronitrosoguanidina/análogos & derivados , Camundongos , Neoplasias Gástricas/induzido quimicamenteRESUMO
In a previous study (Z. Y. Wang et al., Cancer Res., 52: 1162-1170, 1992), we found that administration of a water extract of green tea leaves as the sole source of drinking fluid inhibited ultraviolet B light (UVB)-induced carcinogenesis in SKH-1 mice previously initiated with 7,12-dimethylbenz[a]anthracene (DMBA). In the present study, we compared the effects of black tea, green tea, decaffeinated black tea, and decaffeinated green tea on UVB-induced skin carcinogenesis in DMBA-initiated SKH-1 mice. A 1.25% water extract of each kind of tea leaf (1.25 g tea leaf/100 ml water) was prepared by passing 4 liters of hot water through 50 g of tea leaves in a Bunn tea brewing machine. The mean concentrations of solids in multiple samples of 1.25% black tea, green tea, decaffeinated black tea, and decaffeinated green tea analyzed during the course of this study were 4.23, 3.94, 3.66, and 3.53 mg/ml, respectively. These concentrations of tea solids are similar to those present in tea brews ingested by humans. Female SKH-1 mice were treated topically with 200 nmol of DMBA, followed 3 weeks later by irradiation with 30 mJ/cm2 of UVB twice weekly for 31 weeks. UVB-induced formation of skin tumors was markedly inhibited by oral administration of 0.63 or 1.25% black tea, green tea, decaffeinated black tea, or decaffeinated green tea as the sole source of drinking fluid 2 weeks prior to and during 31 weeks of UVB treatment. Administration of each of the eight tea preparations not only inhibited the number of tumors, but tumor size was also markedly decreased. Histopathological examination of each tumor showed that oral administration of the eight tea preparations had a marked inhibitory effect on the formation of UVB-induced keratoacanthomas and carcinomas. Administration of 1.25% black tea, green tea, decaffeinated black tea, or decaffeinated green tea inhibited the number of keratoacanthomas per mouse by 79, 78, 73, or 70%, respectively, and the number of carcinomas per mouse was inhibited by 93, 88, 77, or 72%, respectively. In summary, administration of black tea was comparable to green tea as an inhibitor of UVB-induced skin carcinogenesis in DMBA-initiated SKH-1 mice. Oral administration of decaffeinated black tea or decaffeinated green tea also had a marked inhibitory effect on UVB-induced skin carcinogenesis in DMBA-initiated SKH-1 mice, but these tea preparations were slightly less effective than the regular teas at the high dose level.
Assuntos
Neoplasias Induzidas por Radiação/prevenção & controle , Extratos Vegetais/farmacologia , Neoplasias Cutâneas/prevenção & controle , Chá , 9,10-Dimetil-1,2-benzantraceno , Administração Oral , Animais , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Camundongos , Camundongos Pelados , Camundongos Endogâmicos , Extratos Vegetais/administração & dosagem , Neoplasias Cutâneas/induzido quimicamente , Raios UltravioletaRESUMO
The effect of p.o. administration of tea on nitrosamine-induced carcinogenesis was investigated. Female A/J mice were given N-nitrosodiethylamine (NDEA) (10 mg/kg) p.o. once a week for 8 weeks and were killed 16 weeks after the last dose. More than 90% of the mice had forestomach and lung tumors. The animals had an average of 8.3 forestomach and 2.5 lung tumors/mouse. With 0.63 or 1.25% green tea infusion (12.5 g green tea leaves brewed with 1 liter of boiling water) as the sole source of drinking water for the entire experimental period, the pulmonary tumor incidence was decreased by 18 or 44%, and the tumor multiplicity was reduced by 36 or 60%, respectively. The treatments also decreased the forestomach tumor incidence by 18 or 26% and tumor multiplicity by 59 or 63%, respectively. Administration of 0.63 or 1.25% green tea infusion, either during the NDEA treatment period only or starting 1 week after the completion of NDEA treatment, also decreased the pulmonary tumor incidence and multiplicity and the forestomach tumor multiplicity. The inhibitory effects of green tea infusion were also observed in a similar experiment using a higher dosage of NDEA (20 mg/kg). Treatment of female A/J mice with a single dose (103 mg/kg) of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) resulted in the formation of pulmonary adenomas in almost all of the animals with an average of 9.3 tumors/mouse after 16 weeks. When 0.6% decaffeinated green tea or black tea extract was given during the NNK-treatment period, tumor multiplicity was reduced by 67 or 65%, respectively. When the tea extract was given after the NNK-treatment period until the end of the experiment, 0.6% green tea extract decreased the tumor incidence and multiplicity by 30 and 85%, respectively. In this protocol, 0.6% black tea extract reduced tumor multiplicity by about 63% but did not significantly affect the tumor incidence. The results clearly demonstrated an inhibitory action of green tea and black tea on nitrosamine-induced tumorigenesis.
Assuntos
Carcinógenos/toxicidade , Dietilnitrosamina/toxicidade , Neoplasias Pulmonares/prevenção & controle , Nitrosaminas/toxicidade , Neoplasias Gástricas/prevenção & controle , Chá , Animais , Feminino , Neoplasias Pulmonares/induzido quimicamente , Neoplasias Pulmonares/patologia , Camundongos , Camundongos Endogâmicos A , Valores de Referência , Neoplasias Gástricas/induzido quimicamente , Neoplasias Gástricas/patologiaRESUMO
In 10 separate experiments, mice with established chemically induced or UV light-induced skin papillomas were treated continuously with green tea in the drinking water or with i.p. injections of a green tea polyphenol fraction or (-)-epigallocatechin gallate three times a week for 4-10 weeks. Partial tumor regression or > 90% inhibition of tumor growth, as measured by changes in tumor volume per mouse, was observed in 5 experiments, and marked inhibition of tumor growth (46-89%) was observed in 5 additional experiments. Treatment of the mice with green tea or green tea constituents had an inhibitory effect on body weight increases in several but not all of the studies. Examination of the data from all ten experiments revealed that complete tumor regression occurred in 14 of 346 papilloma-bearing mice (4%) that were treated with green tea in the drinking water or with i.p. injections of green tea constituents, whereas none of the 220 papilloma-bearing control mice treated with only vehicle exhibited complete tumor regression. These observations indicate that oral administration of green tea, i.p. administration of a green tea polyphenol fraction, or i.p. administration of (-)-epigallocatechin gallate inhibited the growth and/or caused the regression of established experimentally induced skin papillomas.
Assuntos
Catequina/análogos & derivados , Papiloma/tratamento farmacológico , Neoplasias Cutâneas/tratamento farmacológico , Chá , 9,10-Dimetil-1,2-benzantraceno , Animais , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Flavonoides/uso terapêutico , Injeções Intraperitoneais , Camundongos , Papiloma/induzido quimicamente , Papiloma/patologia , Fenóis/uso terapêutico , Extratos Vegetais/uso terapêutico , Polímeros/uso terapêutico , Neoplasias Cutâneas/induzido quimicamente , Neoplasias Cutâneas/patologia , Chá/química , Acetato de TetradecanoilforbolRESUMO
Developmental exposure to methylmercury (MeHg) induces a spectrum of neurological impairment characterized by cognitive disturbance, sensory/motor deficit, and diffuse structural abnormalities of the brain. These alterations may arise from neural path-finding errors during brain development, resulting from disturbances in the function of morphoregulatory guidance molecules. The Eph family of tyrosine kinase receptors and their ligands, the ephrins, guide neuronal migration and neurite pathfinding mainly via repulsive intercellular interactions. The present study examined the effects of MeHg on mRNA and protein expression profiles of Ephs and ephrins in the P19 embryonal carcinoma (EC) cell line and its neuronal derivatives. Undifferentiated control P19 cells displayed low- to undetectable levels of mRNA for ephrins or Ephs, with the sole exception of EphA2 which was highly expressed. Upon differentiation into neurons, the ephrin expression increased progressively through day 10. Similarly, expression of the Ephs, including EphsA3, -A4, -A8, -B2, -B3, -B4, and -B6, increased significantly. In contrast, EphA2 expression decreased in day 2, 6 and 10 control neurons. Treatment with MeHg did not affect the expression of mRNA for ephrins or Ephs in undifferentiated P19 cells. However, treatment of differentiating neurons with MeHg for 24 h caused consistent increases in ligand mRNA expression, particularly ephrin-A5, -A6, -B1, and -B2. Similarly, MeHg induced variable increases in mRNA expression of receptors EphA2, -A3, -B3, and -B6. A trend toward a concentration-response relationship was observed for the alterations in Eph receptor mRNA expression although increases at the low and mid concentrations did not reach statistical significance. Immunoblots for ligand and receptor proteins mirrored the increases in the mRNA levels at the 0.5 and 1.5 microM MeHg concentrations but showed decreased protein levels compared to controls at the 3.0 microM concentration. Alterations in the Eph/ephrin family of repulsion molecules may represent an important mechanism in developmental MeHg neurotoxicity.
Assuntos
Efrinas/biossíntese , Compostos de Metilmercúrio/farmacologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Neurônios/fisiologia , Receptores da Família Eph/biossíntese , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Células-Tronco de Carcinoma Embrionário , Efrinas/genética , Humanos , Immunoblotting , Hibridização In Situ , Ligantes , Neurônios/efeitos dos fármacos , Ensaios de Proteção de Nucleases , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , RNA Neoplásico/análise , RNA Neoplásico/biossíntese , Receptores da Família Eph/genéticaRESUMO
Treatment of P19 embryonal carcinoma cells with retinoic acid induces their differentiation into a population of cells consisting of neurons and other cell types normally derived from neuroectoderm. We used immunohistological and histochemical techniques to identify some of the neurotransmitters in the P19-derived neurons. The majority of neurons contained GABA, glutamic acid decarboxylase, and GABA-transaminase. Neuropeptide Y and somatostatin were less frequently found and both were partially co-expressed with GABA and with one another. Smaller numbers of cells were positive for tyrosine hydroxylase, DOPA decarboxylase, serotonin, calcitonin gene-related peptide, galanin and substance P. The variety and proportions of cells with different transmitter types were reproducible from one experiment to the next and varied very little over 40 days in culture except for cells containing enkephalin, which were abundant only in mature cultures of 32 days or more. Synapses formed between neurons and some contained both small clear and large dense-core vesicles within the presynaptic bouton. Because GABA, neuropeptide Y and somatostatin are abundant in P19-derived neurons as well as in embryonic neurons in rostral regions of the mammalian CNS, we suggest that the developmental events occurring in P19 cell cultures closely resemble those of the embryonic neuroectoderm.
Assuntos
Neurônios/fisiologia , Neurônios/ultraestrutura , Neurotransmissores/fisiologia , Animais , Carcinoma Embrionário/ultraestrutura , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Imunofluorescência , Imuno-Histoquímica , Masculino , Camundongos , Microscopia Eletrônica , Neurônios/efeitos dos fármacos , Neuropeptídeos/fisiologia , Sinapses/efeitos dos fármacos , Sinapses/ultraestrutura , Tretinoína/farmacologia , Células Tumorais CultivadasRESUMO
Several investigators have suggested that certain hydroxylated metabolites of 17beta-estradiol (E2) are the proximate carcinogens that induce mammary carcinomas in estrogen-sensitive rodent models. The studies reported here were designed to examine the carcinogenic potential of different levels of E2 and the effects of genotoxic metabolites of E2 in an in vivo model sensitive to E2-induced mammary cancer. The potential induction of mammary tumors was determined in female ACI rats subcutaneously implanted with cholesterol pellets containing E2 (1, 2, or 3 mg), or 2-hydroxyestradiol (2-OH E2), 4-hydroxyestradiol (4-OH E2), 16alpha-hydroxyestradiol (16alpha-OH E2), or 4-hydoxyestrone (4-OH E1) (equimolar to 2 mg E2). Treatment with 1, 2, or 3 mg E2 resulted in the first appearance of a mammary tumor between 12 and 17 weeks, and a 50% incidence of mammary tumors was observed at 36, 19, and 18 weeks respectively. The final cumulative mammary tumor incidence in rats treated with 1, 2, or 3 mg E2 for 36 weeks was 50%, 73%, and 100% respectively. Treatment of rats with pellets containing 2-OH E2, 4-OH E2, 16alpha-OH E2, or 4-OH E1 did not induce any detectable mammary tumors. The serum levels of E2 in rats treated with a 1 or 3 mg E2 pellet for 12 weeks was increased 2- to 6-fold above control values (approximately 30 pg/ml). Treatment of rats with E2 enhanced the hepatic microsomal metabolism of E2 to E1, but did not influence the 2- or 4-hydroxylation of E2). In summary, we observed a dose-dependent induction of mammary tumors in female ACI rats treated continuously with E2; however, under these conditions 2-OH E2, 4-OH E2, 16alpha-OH E2, and 4-OH E1 were inactive in inducing mammary tumors.
Assuntos
Carcinoma in Situ/induzido quimicamente , Carcinoma Ductal de Mama/induzido quimicamente , Estradiol/análogos & derivados , Estrogênios/toxicidade , Neoplasias Mamárias Experimentais/induzido quimicamente , Animais , Relação Dose-Resposta a Droga , Implantes de Medicamento , Estradiol/toxicidade , Estriol/toxicidade , Estrogênios de Catecol , Feminino , Hidroxiestronas/toxicidade , Ratos , Ratos Endogâmicos ACIRESUMO
Brain dysmorphogenesis and persistent psychomotor disturbances are hallmarks of developmental methylmercury (MeHg) exposure, but the molecular mechanisms underlying these effects are poorly understood. Targets of developmental MeHg exposure include neural cell adhesion molecules (NCAMs), sialoglycoconjugate molecules whose proper temporal and spatial expression is important at all stages of neurodevelopment and especially during synaptic structuring. To investigate the effects of MeHg on the temporal expression of NCAM during development, rat pups were dosed with 7.0 mg/kg MeHgCl (s.c.) on alternate days from postnatal days (PNDs) 3-13 and killed on PNDs 15, 30 and 60. Brain MeHg concentrations were determined in a subset of litters injected with CH(3)203Hg. Expression of NCAM180 protein and of NCAM180 polysialylation was examined in whole cerebellum homogenates, cerebellar synaptosomes and isolated cerebellar growth cones by Western blotting and immunocytochemical staining. NCAM sialyltransferase activity was assayed in preparations of purified Golgi apparatus from the cerebelli of rats treated in vivo, or following in vitro incubation with 0, 1, 2.5, or 7.5 microM MeHg for 2 h. At PND15, no change in NCAM180 protein expression was observed in any cerebellar preparations, but decreased polysialylation of NCAM180 was observed in cerebellar whole homogenates, synaptosomes and isolated growth cones. At PND30, both NCAM180 protein expression and NCAM180 polysialylation were elevated in whole homogenate preparations but not in synaptosomes. NCAM180 expression in MeHg-treated rats was similar to controls at PND60, 47 days after the last methylmercury administration. In vivo studies of cerebellar Golgi sialyltransferase activity revealed significant reductions in PND15 MeHg-treated rats as compared to controls, but no changes in sialyltransferase activity in PND30 and PND60 animals. In vitro experiments revealed decreasing sensitivity of cerebellar sialyltransferases to MeHg as the developmental age of the rat increased. Toxic perturbation of the developmentally-regulated expression of polysialylated NCAM during brain formation may disturb the stereotypic formation of neuronal contacts and could contribute to the behavioral and morphological disturbances observed following MeHg poisoning.
Assuntos
Cerebelo/efeitos dos fármacos , Complexo de Golgi/enzimologia , Compostos de Metilmercúrio/toxicidade , Moléculas de Adesão de Célula Nervosa/biossíntese , Ácidos Siálicos/biossíntese , Sialiltransferases/metabolismo , Animais , Cerebelo/enzimologia , Cerebelo/crescimento & desenvolvimento , Ativação Enzimática/efeitos dos fármacos , Feminino , Complexo de Golgi/efeitos dos fármacos , Compostos de Metilmercúrio/farmacocinética , Moléculas de Adesão de Célula Nervosa/análise , Neurotoxinas/toxicidade , Ratos , Ratos Sprague-Dawley , Ácidos Siálicos/análise , Ácidos Siálicos/metabolismo , Sinaptossomos/química , Sinaptossomos/efeitos dos fármacosRESUMO
Pluripotent embryonal carcinoma (P19) cells differentiate into a neural phenotype in response to retinoic acid (RA). Expression of the low and medium molecular weight neurofilament subunits, but not the high molecular weight subunit (NFH), has been reported following RA treatment. In this study NFH expression was detected by Western blotting and immunofluorescence microscopy, but lagged behind the expression of the other subunits in a manner similar to that reported during in vivo neuronal development.
Assuntos
Carcinoma Embrionário , Proteínas de Neurofilamentos/biossíntese , Neurônios/metabolismo , Neurônios/ultraestrutura , Animais , Anticorpos , Diferenciação Celular , Movimento Celular , Técnica Indireta de Fluorescência para Anticorpo , Neuritos/metabolismo , Proteínas de Neurofilamentos/análise , Proteínas de Neurofilamentos/imunologia , Teratoma , Células Tumorais CultivadasRESUMO
Trimethyltin (TMT) is a potent neuronotoxiciant but there is little data regarding its systemic effects. In this study, female BALB/c mice were administered either 0.9% saline or 2.75 mg TMT/kg intraperitoneally (i.p.). The animals were then housed in room air or in glass chambers flushed with either 10%, 40%, or 100% oxygen. Mice were sacrificed at 4, 8, 24, and 48 h after treatment and adrenals analyzed for various neurotransmitters by ion-pairing HPLC with electrochemical detection. In addition, adrenal S-adenosylmethionine (SAM) and blood ketone bodies were determined Sections of adrenals were evaluated by electron microscopy for histopathological changes. In vivo treatment with the toxicant resulted in a significant decrease in adrenal epinephrine and norepinephrine levels as early as 8 h following treatment. This effect preceded the appearance of both clinical signs and histopathological changes in the hippocampus by 12-24 h. With exposure to TMT in room air, mouse adrenal content of epinephrine fell from 1861.3 +/- 97.3 ng/4 mg to 1493.3 +/- 137.0 ng/4 mg while norepinephrine levels fell from 779.6 +/- 32.3 ng/4 mg to 503.4 +/- 44.3 ng/4 after 8 h. Supplementation with 40% oxygen did not attenuate this effect but in the case of mice treated with TMT and housed in 100% oxygen for 48 h, actually exacerbated the adrenal epinephrine depletion. Housing in approximately half normal atmospheric oxygen (10%) neither prevented nor enhanced the effects of TMT. The epinephrine/norepinephrine ratios were: control, 2.44; TMT (room air), 1.56; TMT (10% O2), 1.72; TMT (40% O2), 1.44; TMT (100% O2), 1.07. None of the conditions used in this study caused a decrease in adrenal dopamine, 5-hydroxyindole acetic acid (5-HIAA), 5-hydroxytryptamine (5-HT) or in the level of SAM. TMT treatment significantly increased blood ketone bodies indicating additional metabolic dysfunction. The significance of these findings in relation to TMT neuronotoxicity and fatty liver syndrome are discussed.
Assuntos
Glândulas Suprarrenais/análise , Catecolaminas/análise , Compostos de Trialquitina/toxicidade , Compostos de Trimetilestanho/toxicidade , Ácido 3-Hidroxibutírico , Glândulas Suprarrenais/efeitos dos fármacos , Animais , Cromatografia Líquida de Alta Pressão , Feminino , Hidroxibutiratos/sangue , Injeções Intraperitoneais , Camundongos , Camundongos Endogâmicos BALB C , Oxigênio/uso terapêuticoRESUMO
Neurobehavioral testing of herring gull chicks (Larus argentatus) in both laboratory and field studies indicates that lead exposure during critical periods of development causes neurological deficits that may compromise survival in the wild. Accumulating evidence suggests that lead impairs neurodevelopment, in part, by altering the expression of cell adhesion molecules (CAMs) responsible for the proper formation and maintenance of neural structure and synaptic function. We examined the adhesion molecules NCAM, L1, and N-cadherin in gull brains to determine whether these CAMs are altered by lead exposure and might serve as markers of developmental neurotoxicity. One-day-old chicks were collected from nesting colonies and were laboratory housed. On post-hatching day (PHD) 2, chicks were given 100 mg/kg lead acetate or saline (intraperitoneally). Birds were killed on PHD 34, 44, or 55 (blood-lead levels averaged 27.4, 20.8, and 19.5 microg/dl, respectively). Brains were removed and stored at -70 degrees C until analysis. Expression of CAMs was determined in synaptosomal preparations by Western blotting and the activity of NCAM-associated sialyltransferase (ST) was determined in purified whole brain golgi apparatus. Elevation in synaptosomal polysialylated NCAM expression and a significant increase in golgi ST activity was observed in lead-treated animals at PHD 34. Reductions in synaptosomal N-cadherin were observed at PHD 34 and 44, while L1 expression appeared unaffected by lead at any time-point. By 55 days post-hatching, no differences in N-cadherin expression, polysialylated NCAM expression or NCAM-associated ST activity were seen in lead-treated animals as compared with age-matched control animals. Lead-induced disruption of CAM expression during early neurodevelopment may contribute to behavioral deficits observed in herring gulls in both the laboratory and the field, and may serve as a marker for heavy metal exposure during postnatal development.
Assuntos
Aves/metabolismo , Química Encefálica/efeitos dos fármacos , Intoxicação do Sistema Nervoso por Chumbo/metabolismo , Moléculas de Adesão de Célula Nervosa/biossíntese , Sinapses/metabolismo , Animais , Biomarcadores , Western Blotting , Caderinas/metabolismo , Densitometria , Chumbo/sangue , Sialiltransferases/metabolismo , Frações Subcelulares/química , Frações Subcelulares/metabolismo , Sinapses/efeitos dos fármacos , Sinaptossomos/efeitos dos fármacos , Sinaptossomos/metabolismoRESUMO
A prolonged period of latency between toxic exposure and disease is a recognized phenomenon in oncology, but is a relatively new concept in neurotoxicology. Suggestions that exposure to neurotoxic compounds may be followed years or decades later by clinically evident neurological disease have received considerable anecdotal support but little empirical evidence. In recent years, evidence has emerged linking environmental factors to several neurodegenerative diseases, including amyotrophic lateral sclerosis and the Parkinsonism-dementia complex. While these diseases have not been definitively demonstrated to arise from exposure to toxins or toxicants, nevertheless the possibility exists that xenobiotic exposure could lead to neurological conditions possessing a period of clinical "silence" prior to expression. Detection of such damage using standard toxicology testing protocols poses a major challenge. Lessons learned from neurodegenerative diseases can be profitably applied to the study of the long-term effects of xenobiotics on the brain and elucidation of the role of aging on the expression of neurotoxicity.
Assuntos
Doenças do Sistema Nervoso/induzido quimicamente , Envelhecimento/efeitos dos fármacos , Animais , Humanos , Fatores de TempoRESUMO
The literature concerning the developmental neuropathology of organotins is reviewed. To date, neuropathological effects have been convincingly demonstrated for trimethyltin (TMT) and triethyltin (TET). Both compounds may damage the developing CNS; however they have different cellular targets. TMT is a neuronotoxin which damages areas of the limbic system, cerebral cortex, and brainstem. TET is a myelinotoxin which causes massive myelinic edema by yet undetermined mechanisms. Neuronal death is also seen following TET intoxication during the neonatal period, possibly as a result of elevated intracranial pressure. The neuropathological features of TMT and TET intoxication during early life are presented. Neurochemical and behavioral alterations resulting from congenital and/or neonatal exposure to organotins are also discussed.
Assuntos
Doenças do Sistema Nervoso/induzido quimicamente , Compostos Orgânicos de Estanho/toxicidade , Animais , Animais Recém-Nascidos , Animais Lactentes , Edema Encefálico/induzido quimicamente , Feminino , Morte Fetal/induzido quimicamente , Reabsorção do Feto/induzido quimicamente , Hipocampo/patologia , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Doenças do Sistema Nervoso/patologia , Gravidez , Complicações na Gravidez , Ratos , Compostos de Trietilestanho/toxicidade , Compostos de Trimetilestanho/intoxicação , Compostos de Trimetilestanho/toxicidadeRESUMO
Electrophysiological function and morphology of dorsal root ganglion (DRG) neurons were investigated in female Wistar rats following administration of methylmercury (5 mg/kg/day for 10 days). Neurons were classified according to the conduction velocities of their peripheral axons and perikaryal action potential characteristics as types A alpha, A beta A delta and C. In methylmercury-treated rats, the majority of DRG neuron action potential characteristics were not significantly altered. However, axonal conduction velocities were slowed in all type A ganglion cells, leading to significant delays in action potential onset in types A beta and A delta neurons. An initial complex-soma inflection was observed on the rising phase of the action potential of some cells. These inflection potentials had longer conduction times and lower amplitudes in treated animals. Repetitive discharge, characterized by 3-5 action potentials upon single peripheral nerve stimulation, was observed in 8 of 38 cells recorded in treated animals, predominantly in type A beta (4/8) and A beta (3/12) neurons; direct current injection into repetitively discharging cells via the recording electrode elicited only single action potentials. Extracellular recordings from dorsal root filaments confirmed that the repetitive action potentials arising in the DRG were conducted to the spinal cord. Morphological examination of DRG revealed damage in up to 10% of neurons, consisting of loss of Nissl staining, cytoplasmic vacuolization, and proliferation of satellite cells. Large, pale neurons appeared preferentially involved. Rarely, neuronophagia was observed. Further microscopic examination of proximal and distal sections of sciatic and tibial nerves revealed only occasional degeneration of myelinated axons. While morphological alterations did not appear to offer a structural basis for the multiple firing, it is speculated that repetitive action potential discharge may contribute to the paresthesias associated with MeHg intoxication.
Assuntos
Gânglios Espinais/efeitos dos fármacos , Compostos de Metilmercúrio/intoxicação , Neurônios/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Animais , Feminino , Gânglios Espinais/patologia , Gânglios Espinais/fisiologia , Neurônios/patologia , Neurônios/fisiologia , Ratos , Ratos WistarRESUMO
The brain relies upon numerous morphoregulatory molecules to control cell-cell interactions, cell migration and neurite extension. N-cadherin, a calcium-dependent cell adhesion molecule, is essential for normal CNS development. Homophilic binding of N-cadherin depends upon a specific conformation assumed by the molecule when it binds calcium. N-cadherin is a substrate for a specific zinc-dependent protease that may be involved in the regulation of N-cadherin at the cell surface. The reliance of N-cadherin on two cations for proper function makes it a potential target for toxicants which act by replacing or modifying calcium or zinc at ion-binding sites. Exposure of the developing brain to lead, an ubiquitous toxicant known to interact with calcium, disturbs neural tube closure and subsequent maturation of the nervous system. Preliminary data indicates that lead may induce these effects by direct interaction with N-cadherin. Numerous common toxicants, including metals and solvents, also perturb cadherins and cause defective CNS development. These data indicate that changes in the spatio-temporal expression of cadherin can result in profound alterations in neural structure and function, and may underlie CNS malformations caused by numerous toxic agents.
Assuntos
Química Encefálica/fisiologia , Encefalopatias/metabolismo , Encéfalo/crescimento & desenvolvimento , Caderinas/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Química Encefálica/efeitos dos fármacos , Caderinas/isolamento & purificação , Cálcio/farmacologia , Adesão Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Eletroforese em Gel de Poliacrilamida , Chumbo/toxicidade , Retina/citologiaRESUMO
Normal development of the nervous system depends upon complex physical interactions between cells and their local environment. These interactions are mediated by several families of cell adhesion molecules (CAMs). Differential expression and function of CAMs are operative in neural tube formation, neuron migration, in post-migratory differentiation, and maintenance of mature neural structure. CAMs also facilitate contact-dependent cell processes, such as formation of cell junctions. Temporal regulation of these molecules during development may provide "windows of vulnerability" to toxicants. In addition to their extracellular binding activities, some CAMs have membrane-spanning domains by which they communicate directly with the cytoskeleton, permitting extracellular signals to be rapidly translated into cell responses via modifications in cytoskeletal organization. These cytologic changes are particularly critical during migration, neurite formation and synaptogenesis. Toxic perturbation of adhesion molecules can have catastrophic effects on morphogenetic processes both directly and via events which depend upon cytoskeletal rearrangement. Toxicants can also act directly upon the cytoskeleton, resulting secondarily in changes of the membrane distribution and function of CAMs. Toxicant-induced changes in CAMs and cytoskeleton may occur contemporaneously. Interference of cell adhesion-cytoskeleton interactions may be a pivotal molecular event dictating developmental consequences of neurotoxicant exposure.
Assuntos
Moléculas de Adesão Celular Neuronais/efeitos dos fármacos , Citoesqueleto/efeitos dos fármacos , Doenças do Sistema Nervoso/induzido quimicamente , Animais , Humanos , Sistema Nervoso/crescimento & desenvolvimento , Doenças do Sistema Nervoso/fisiopatologiaRESUMO
Microtubule (Mt) populations show large differences in dynamic properties (i.e., turnover rates) among cell types, and even within the same cell type at different stages of the cell cycle or stages of differentiation. These differences in dynamic properties are correlated with altered sensitivity to Mt-disassembling drugs (e.g. colchicine) which bind specifically to the Mt protein tubulin and to certain toxic metals which also interact with tubulin (e.g. methylmercury) and result in Mt disassembly. Mts in neurons become progressively more stable and more resistant to such compounds during differentiation. We are using the P19 embryonal carcinoma cell line, which can be induced to differentiate along the neural pathway by retinoic acid, as a model system in which to analyze the development of stable Mts. Our results show that during differentiation there is an evolution in the sorting of tubulin isotypes into the stable Mts. This appears related both to the expression of specific Mt-associated proteins and to concomitant posttranslational modifications of tubulin.
Assuntos
Microtúbulos/fisiologia , Neurônios/fisiologia , Animais , Humanos , Camundongos , Microtúbulos/efeitos dos fármacos , Microtúbulos/metabolismo , Neurônios/metabolismo , Neurônios/ultraestrutura , Tubulina (Proteína)/metabolismo , Células Tumorais CultivadasRESUMO
Cellular heterogeneity and structural complexity of the nervous system, coupled with regional and cellular differences in the metabolic capabilities of neurons, glia and other non-neuronal elements, may underlie the selective cellular involvement following exposure to neurotoxicants. Determination of the role of biotransformation of xenobiotics in neural pathoclisis requires an understanding of the cellular distribution of both phase I and phase II enzyme systems in the brain. While ependyma, choroid plexus and endothelial linings of blood vessels throughout the nervous system appear to contain appreciable amounts of several isoforms of cytochrome P450 (CYP450), glia and neurons tend to be highly specific in which forms of CYP450 they express. Regional and cellular heterogeneity similarly characterize the distribution of glutathione (GSH) and the conjugating enzyme glutathione S-transferase (GST) in the brain. While all cells of the embryonic nervous system express high levels of GSH and pi-GST (with lesser amounts of alpha- and mu-class), by adulthood neurons and non-neuronal cells differ in the distribution of GSH and isoforms of GST. Neurons (except the dorsal root ganglia and the cerebellar granule cells) become GSH-negative but contain alpha-, mu-, and pi-GST. Glia, ependyma, choroid plexus and neurovascular cells are rich in GSH and variously express GSTs. The differences found in the cellular distribution of GSH and GSTs may contribute to changes in the vulnerability of the nervous system to neurotoxicants at different ages. A comprehensive understanding of the neurotoxicological and pharmacological consequences of the cellular heterogeneity in the localization the CYP450s and glutathione S-transferases pre- and postnatally will require systematic study of the distribution, substrate specificity, and inducibility of the various isoforms of these enzyme systems.